The quest for Magrathea planets I: formation of second generation exoplanets around double white dwarfs

The evolution of binaries that become double white dwarf (DWD) can cause the ejection of high amounts of dust and gas. Such material can give rise to circumbinary discs and become the cradle of new planets, yet no studies so far have focused on the formation of circumbinary planets around DWDs. These binaries will be the main sources of gravitational waves (GWs) detectable by the ESA Laser Interferometer Space Antenna (LISA) mission, opening the possibility to detect circumbinary planets around short-period DWDs everywhere in the Milky Way. We investigate the formation of Magrathea planets by simulating multiple planet formation tracks to explore how seeds growing first by pebble accretion, and then by gas accretion, are affected by the disc environments surrounding DWDs. We present both planetary formation tracks taking place in steady-state discs, and formation tracks taking place in discs evolving with time. The time-dependent tracks account for both the disc accretion rate onto the central binary and the disc photoevaporation rate caused by stellar irradiation. Our results show that planetary formation in circumbinary discs around DWDs can be possible. In particular, the extreme planetary formation environment implies three main significant results: (i) the accretion rate and the metallicity of the disc should be high in order to form sub-stellar objects with masses up to 31 M$_J$, this is achieved only if planet formation starts soon after the onset of the disc and if first generation seeds are present in the disc; (ii) seeds formed within 0.1 Myr, or within 1 Myr, from the onset of the disc can only produce sub-Neptune and Neptunian planets, unless the disc accommodates first generation seeds with mass 10 M$_{\oplus}$; (iii) most of the planets are finally located within 1 au from the disc centre, while they are still undergoing the gas accretion phase.Comment: Accepted for publication in A&A on 05/04/2023, abstract shortened, 28 pages, 11 figures, 14 table

Evaluation of the energy utilization index in sheep milk cooling systems

The energy consumption of sheep milk cooling systems (MCSs) was quantified in this study to provide original information filling a literature gap on the impact of sheep milk cooling on the energy and economic balance in dairy farms. Performance and energy monitoring tests were conducted simultaneously on 22 MCSs in Sardinia (Italy). The results determined the cooling time as a function of the performance class and number of milkings. The Energy Utilization Index (EUI) was applied to measure the energy required to cool down the milk and estimate the incidence on its price. The average EUI was 1.76 kWh 100 L−1 for two-milkings and 2.43 kWh 100 L−1 for four-milkings MCSs, whereas the CO2 emissions ranged from 998 to 1378 g CO2 100 L−1 for two- and four-milkings MCSs, respectively. The estimated energy consumption for the storage of refrigerated sheep milk was 0.12 kWh 100 L−1. The malfunctioning MCSs averagely consumed 31% more energy than regular systems. The energy cost for cooling accounted for 0.61% on the current sheep milk price in Italy. Based on the analysis, the reported EUI values can be used as a preliminary indicator of the regular operation of MCSs

The Conserved nhaAR Operon Is Drastically Divergent between B2 and Non-B2 Escherichia coli and Is Involved in Extra-Intestinal Virulence

The Escherichia coli species is divided in phylogenetic groups that differ in their virulence and commensal distribution. Strains belonging to the B2 group are involved in extra-intestinal pathologies but also appear to be more prevalent as commensals among human occidental populations. To investigate the genetic specificities of B2 sub-group, we used 128 sequenced genomes and identified genes of the core genome that showed marked difference between B2 and non-B2 genomes. We focused on the gene and its surrounding region with the strongest divergence between B2 and non-B2, the antiporter gene nhaA. This gene is part of the nhaAR operon, which is in the core genome but flanked by mobile regions, and is involved in growth at high pH and high sodium concentrations. Consistently, we found that a panel of non-B2 strains grew faster than B2 at high pH and high sodium concentrations. However, we could not identify differences in expression of the nhaAR operon using fluorescence reporter plasmids. Furthermore, the operon deletion had no differential impact between B2 and non-B2 strains, and did not result in a fitness modification in a murine model of gut colonization. Nevertheless, sequence analysis and experiments in a murine model of septicemia revealed that recombination in nhaA among B2 strains was observed in strains with low virulence. Finally, nhaA and nhaAR operon deletions drastically decreased virulence in one B2 strain. This effect of nhaAR deletion appeared to be stronger than deletion of all pathogenicity islands. Thus, a population genetic approach allowed us to identify an operon in the core genome without strong effect in commensalism but with an important role in extra-intestinal virulence, a landmark of the B2 strains

Ultrasound IMT measurement on a multi-ethnic and multi-institutional database: Our review and experience using four fully automated and one semi-automated methods

Automated and high performance carotid intima-media thickness (IMT) measurement is gaining increasing importance in clinical practice to assess the cardiovascular risk of patients. In this paper, we compare four fully automated IMT measurement techniques (CALEX, CAMES, CARES and CAUDLES) and one semi-automated technique (FOAM). We present our experience using these algorithms, whose lumen-intima and media-adventitia border estimation use different methods that can be: (a) edge-based; (b) training-based; (c) feature-based; or (d) directional Edge-Flow based. Our database (DB) consisted of 665 images that represented a multi-ethnic group and was acquired using four OEM scanners. The performance evaluation protocol adopted error measures, reproducibility measures, and Figure of Merit (FoM). FOAM showed the best performance, with an IMT bias equal to 0.025 ± 0.225 mm, and a FoM equal to 96.6%. Among the four automated methods, CARES showed the best results with a bias of 0.032 ± 0.279 mm, and a FoM to 95.6%, which was statistically comparable to that of FOAM performance in terms of accuracy and reproducibility. This is the first time that completely automated and user-driven techniques have been compared on a multi-ethnic dataset, acquired using multiple original equipment manufacturer (OEM) machines with different gain settings, representing normal and pathologic case

Agricultural sustainability estimation of the European photovoltaic greenhouses

The integration of the photovoltaic (PV) energy in the greenhouse farm has raised concerns on the agricultural sustainability of this specific agrosystem in terms of crop planning and management, due to the shading cast by the PV panels on the canopy. The PV greenhouse (PVG) can be classified on the basis of the PV cover ratio (PVR), that is the ratio of the projected area of PV panels to the ground and the total greenhouse area. In this paper, we estimated the yield of 14 greenhouse horticultural and floricultural crops inside four commercial PVG types spread in southern Europe, with PVR ranging from 25 to 100%. The aim of the work is to identify the PVG types suitable for the cultivation of the considered species, based on the best trade-off between PV shading and crop production. The daily light integral (DLI) was used to compare the light scenarios inside the PVGs to the crop light requirements, and estimate the potential yield. The structures with a PVR of 25% were compatible with the cultivation of all considered species, including the high light demanding ones (tomato, cucumber, sweet pepper), with an estimated negligible or limited yield reduction (below 25%). The medium light species (such as asparagus) with an optimal DLI lower than 17 mol m−2 d−1 and low light crops can be cultivated inside PVGs with a PVR up to 60%. Only low light demanding floricultural species with an optimal DLI lower than 10 mol m−2 d−1, such as poinsettia, kalanchoe and dracaena, were compatible inside PVGs with a PVR up to 100%. Innovative cropping systems should be considered to overcome the penalizing light scenarios of the PVGs with high PVR, also implementing LED supplementary lighting. This paper contributes to identify the sustainable PVG types for the chosen species and the alternative crop managements in terms of transplantation period and precision agriculture techniques, aimed at increasing the crop productivity and adaptability inside the PVG agrosystems

Linomide blocks angiogenesis by breast carcinoma vascular endothelial growth factor transfectants.

The blocking of angiogenesis provides a novel therapeutic target to inhibit tumour spreading. In this study, we investigated the effect of linomide on angiogenesis induced in vivo by highly angiogenic breast carcinoma cells. The rabbit cornea was used to assess neovascular growth in the absence of a tumour mass. MCF-7 cells stably transfected with the cDNA encoding for vascular endothelial growth factor 121 (VEGF121) (V12 clone) were used to elicit a potent VEGF-dependent corneal angiogenesis. After tumour cell implant, albino rabbits received 100 mg kg(-1) day(-1) linomide for 5 consecutive days. Daily observation of neovascular progression indicated that linomide blocked angiogenesis. The antiangiogenic effect of linomide was apparent within 48 h from the beginning of the treatment and was both angiosuppressive and angiostatic. The block of neovascular growth lasted over 10 days from treatment suspension, and preformed vessels, which had regressed, remained dormant, suggesting the persistence of unfavourable conditions for capillary progression. Linomide (50-200 microg ml[-1]) was not cytotoxic in vitro on resting capillary endothelial cells but blocked endothelial cell replication induced by VEGF. Our data indicate that linomide can efficiently and persistently block VEGF-dependent angiogenesis in vivo in the absence of a growing tumour mass. These data suggest that linomide could be a chemopreventive drug in breast cancer patients and a valuable tool in clinical settings in which metastatic spreading occurs in the absence of a detectable tumour mass

Permeability sets the linear path instability of buoyancy-driven disks

The prediction of trajectories of buoyancy-driven objects immersed in a viscous fluid is a key problem in fluid dynamics. Simple-shaped objects, such as disks, present a great variety of trajectories, ranging from zig-zag to tumbling and chaotic motions. Yet, similar studies are lacking when the object is permeable. We perform a linear stability analysis of the steady vertical path of a thin permeable disk, whose flow through the microstructure is modelled via a stress-jump model based on homogenization theory. The relative velocity of the flow associated with the vertical steady path presents a recirculation region detached from the body, which shrinks and eventually disappears as the disk becomes more permeable. In analogy with the solid disk, one non-oscillatory and several oscillatory modes are identified and found to destabilize the fluid-solid coupled system away from its straight trajectory. Permeability progressively filters out the wake dynamics in the instability of the steady vertical path. Modes dominated by wake oscillations are first stabilized, followed by those characterized by weaker, or absent, wake oscillations, in which the wake is typically a tilting induced by the disk inclined trajectory. For sufficiently large permeabilities, the disk first undergoes a non-oscillatory divergence instability, which is expected to lead to a steady oblique path with a constant disk inclination, in the nonlinear regime. A further permeability increase reduces the unstable range of all modes until quenching of all linear instabilities

Cytokeratin-19 positivity is acquired along cancer progression and does not predict cell origin in rat hepatocarcinogenesis

Although the expression of the stem/progenitor cell marker cytokeratin-19 (CK-19) has been associated with the worst clinical prognosis among all HCC subclasses, it is yet unknown whether its presence in HCC is the result of clonal expansion of hepatic progenitor cells (HPCs) or of de-differentiation of mature hepatocytes towards a progenitor-like cell phenotype. We addressed this question by using two rat models of hepatocarcinogenesis: the Resistant-Hepatocyte (R-H) and the Choline-methionine deficient (CMD) models. Our data indicate that the expression of CK-19 is not the result of a clonal expansion of HPCs (oval cells in rodents), but rather of a further step of preneoplastic hepatocytes towards a less differentiated phenotype and a more aggressive behavior. Indeed, although HCCs were positive for CK-19, very early preneoplastic foci (EPFs) were completely negative for this marker. While a few weeks later the vast majority of preneoplastic nodules remained CK-19 negative, a minority became positive, suggesting that CK-19 expression is the result of de-differentiation of a subset of EPFs, rather than a marker of stem/progenitor cells. Moreover, the gene expression profile of CK-19-negative EPFs clustered together with CK-19-positive nodules, but was clearly distinct from CK-19 negative nodules and oval cells. Conclusion: i) CK-19-positive cells are not involved in the early clonal expansion observed in rat hepatocarcinogenesis; ii) CK-19 expression arises in preneoplastic hepatocyte lesions undergoing malignant transformation; iii) CK-19 positivity in HCCs does not necessarily reflect the cell of origin of the tumor, but rather the plasticity of preneoplastic cells during the tumorigenic proces

Mechanisms That Modulate and Diversify BDNF Functions: Implications for Hippocampal Synaptic Plasticity

Brain-derived neurotrophic factor (BDNF) is a neurotrophin that has pleiotropic effects on neuronal morphology and synaptic plasticity that underlie hippocampal circuit development and cognition. Recent advances established that BDNF function is controlled and diversified by molecular and cellular mechanisms including trafficking and subcellular compartmentalization of different Bdnf mRNA species, pre- vs. postsynaptic release of BDNF, control of BDNF signaling by tropomyosin receptor kinase B (TrkB) receptor interactors and conversion of pro-BDNF to mature BDNF and BDNF-propeptide. Defects in these regulatory mechanisms affect dendritic spine formation and morphology of pyramidal neurons as well as synaptic integration of newborn granule cells (GCs) into preexisting circuits of mature hippocampus, compromising the cognitive function. Here, we review recent findings describing novel dynamic mechanisms that diversify and locally control the function of BDNF in hippocampal neurons