135 research outputs found
Rational Design and Synthesis of Large Stokes Shift 2,6-Sulphur-Disubstituted BODIPYs for Cell Imaging
Five new disubstituted 2,6-thioaryl-BODIPY dyes were synthesized via selective aromatic electrophilic substitution from commercially available thiophenols. The analysis of the photophysical properties via absorption and emission spectroscopy showed unusually large Stokes shifts for BODIPY fluorophores (70–100 nm), which makes them suitable probes for bioimaging. Selected compounds were evaluated for labelling primary immune cells as well as different cancer cell lines using confocal fluorescence microscopy
Understanding global changes of the liver proteome during murine schistosomiasis using a label-free shotgun approach.
Schistosomiasis is an endemic disease affecting over 207million people worldwide caused by helminth parasites
of the genus Schistosoma. In Brazil the disease is responsible for the loss of up to 800 lives annually, resulting from
the desabilitating effects of this chronic condition. In this study, we infected Balb/c mice with Schistosoma
mansoni and analysed global changes in the proteomic profile of soluble liver proteins. Our shotgun analyses revealed
predominance of up-regulation of proteins at 5weeks of infection, coincidingwith the onset of egg laying,
and a remarkable down-regulation of liver constituents at 7 weeks, when severe tissue damage is installed. Representatives
of glycolytic enzymes and stress response (in particular at the endoplasmic reticulum)were among
the most differentially expressed molecules found in the infected liver. Collectively, our data contribute over 70
molecules not previously reported to be found at altered levels in murine schistosomiasis to further exploration
of their potential as biomarkers of the disease.Moreover, understanding their intricate interaction using bioinformatics
approach can potentially bring clarity to unknownmechanisms linked to the establishment of this condition
in the vertebrate host.
Significance: To our knowledge, this study refers to the first shotgun proteomic analysis to provide an inventory of
the global changes in the liver soluble proteome caused by Schistosomamansoni in the Balb/cmodel. It also innovates
by yielding data on quantification of the identified molecules as a manner to clarify and give insights into
the underlying mechanisms for establishment of Schistosomiasis, a neglected tropical disease with historical
prevalence in Brazil
Análise da estrutura fatorial dos Testes de Torrance em estudantes portugueses
In order to verify the factorial structures of the Torrance verbal and figural tests, two activities of each instrument were applied with 193 students from the 10th and 12th years of education in Portugal. We tried to demonstrate that the collinearity of the fluency and flexibility variables could create methodological artifacts that hinder the understanding of the internal structure underlying the test. The principal component analysis without control of collinearity indicated a solution composed of four basic factors that separeted activities. Controlling for collinearity, we found a new solution, which also contained four factors that, unlike the previous result, grouped variables with similar processes but of different activities. The verbal and figural content is also an important element in the factor structure. This new arrangement makes more sense with the theory that underlies the instruments separating the different processes and content which are being measured by the activities.Com a finalidade de verificar a estrutura fatorial dos testes de Torrance, duas atividades verbais e duas figurais foram aplicadas em 193
estudantes do 10º e 12º ano do ensino secundário de Portugal. Tentou-se demonstrar que a colinearidade das variáveis fluência e flexibilidade
podem criar artefatos metodológicos que dificultam o entendimento da estrutura interna subjacente ao teste. A análise fatorial dos
componentes principais, sem controle da colinearidade, indicou uma solução composta por quatro fatores que separam basicamente as
atividades. Controlando-se a colinearidade, encontrou-se uma nova solução, também composta por quatro fatores, que, diferentemente
da anterior, organizou variáveis com processos semelhantes, mas de diferentes atividades. O tipo de conteúdo, verbal e figural, mostrou-se
ainda um importante elemento na organização dos fatores. Esse novo arranjo fez mais sentido diante da teoria que embasa os instrumentos,
ao separar os diferentes processos e conteúdos por eles avaliados
Factors associated with the development of early childhood caries among Brazilian preschoolers
Isotopic abundance of 13 C and contribution of eucalyptus biomass to soil organic matter conversion
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii
Background: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The three types have different phenotypes and affect different citrus species. The causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C. Results: We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. In addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein. Conclusion: We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. The gained knowledge will be instrumental for improving citrus canker control.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento CientIfico e Tecnologico (CNPq)Coordenacao para Aperfeicoamento de Pessoal de Ensino Superior (CAPES)Fundo de Defesa da Citricultura (FUNDECITRUS
Produtividade e qualidade de abacaxizeiro cv. Smooth Cayenne, cultivado comaplicação de doses e parcelamentos do nitrogênio, em Guaraçaí-SP
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