Degradation rates and products of pure magnesium exposed to different aqueous media under physiological conditions

As magnesium and many of its alloys are a promising class of degradable implant materials, a thorough understanding of their degradation under physiological conditions is a key challenge in the field of biomaterial science. In order to increase the predictive power of in vitro studies, it is necessary to imitate the in vivo conditions, track the decomposition process and identify the products that form during the degradation pathway. In this in vitro study, slices of pure magnesium were exposed to Hank's Balanced Salt Solution (HBSS), Dulbecco's Modified Eagle Medium (DMEM) and simulated body fluid (SBF), respectively, under cell culture conditions, which included CO2 gassing. The series were repeated with supplements of fetal bovine serum (FBS), added to the respective media. Degradation rates, osmolality and pH were found to vary with the choice of medium and supplementation with proteins. In order to identify the crystalline degradation products, the crusts formed on the specimens were investigated via X-ray diffraction (XRD) measurements. As expected, brucite, Mg(OH)2, was found among the degradation products; interestingly, nesquehonite, Mg(HCO3)(OH)·2H2O, was found to be the dominant degradation product in this study. The experimental data are well in accordance with solubility calculations

Degradation rates and products of pure magnesium exposed to different aqueous media under physiological conditions

As magnesium and many of its alloys are a promising class of degradable implant materials, a thorough understanding of their degradation under physiological conditions is a key challenge in the field of biomaterial science. In order to increase the predictive power of in vitro studies, it is necessary to imitate the in vivo conditions, track the decomposition process and identify the products that form during the degradation pathway. In this in vitro study, slices of pure magnesium were exposed to Hank's Balanced Salt Solution (HBSS), Dulbecco's Modified Eagle Medium (DMEM) and simulated body fluid (SBF), respectively, under cell culture conditions, which included CO2 gassing. The series were repeated with supplements of fetal bovine serum (FBS), added to the respective media. Degradation rates, osmolality and pH were found to vary with the choice of medium and supplementation with proteins. In order to identify the crystalline degradation products, the crusts formed on the specimens were investigated via X-ray diffraction (XRD) measurements. As expected, brucite, Mg(OH)2, was found among the degradation products; interestingly, nesquehonite, Mg(HCO3)(OH)·2H2O, was found to be the dominant degradation product in this study. The experimental data are well in accordance with solubility calculations

Liquid Mirror Telescopes: A progress report

We review the present status of liquid mirror telescopes. Interferometric tests of liquid mirrors (the largest one having a diameter of 2.5 meters ) show excellent optical qualities. The basic technology is now sufficiently reliable that it can be put to work. Indeed, a handful of liquid mirrors have now been built that are used for scientific work. A 3.7-m diameter LMT is presently being built in the new Laval upgraded testing facilities. Construction of the mirror can be followed on the Web site: http://astrosun.phy.ulaval.ca/lmt/lmt- home.html. Finally we address the issue of the field accessible to LMTs equipped with novel optical correctors. Optical design work, and some exploratory laboratory work, indicate that a single LMT should be able to access, with excellent images, small regions anywhere inside fields as large as 45 degrees.Comment: To appear in the SPIE conference proceedings 'Optical Telescopes of Today and Tomorrow', May 29 - June1, Landskrona/Hven, Sweden, Additional info on LMs at: http://astrosun.phy.ulaval.ca/lmt/lmt-home.htm

Degradation rates and products of pure magnesium exposed to different aqueous media under physiological conditions

As magnesium and many of its alloys are a promising class of degradable implant materials, a thorough understanding of their degradation under physiological conditions is a key challenge in the field of biomaterial science. In order to increase the predictive power of in vitro studies, it is necessary to imitate the in vivo conditions, track the decomposition process and identify the products that form during the degradation pathway. In this in vitro study, slices of pure magnesium were exposed to Hank's Balanced Salt Solution (HBSS), Dulbecco's Modified Eagle Medium (DMEM) and simulated body fluid (SBF), respectively, under cell culture conditions, which included CO2 gassing. The series were repeated with supplements of fetal bovine serum (FBS), added to the respective media. Degradation rates, osmolality and pH were found to vary with the choice of medium and supplementation with proteins. In order to identify the crystalline degradation products, the crusts formed on the specimens were investigated via X-ray diffraction (XRD) measurements. As expected, brucite, Mg(OH)2, was found among the degradation products; interestingly, nesquehonite, Mg(HCO3)(OH)·2H2O, was found to be the dominant degradation product in this study. The experimental data are well in accordance with solubility calculations

Contribution à la caractérisation de sites sableux : signature spectro-directionnelle, distribution en taille et minéralogie extraites d'échantillons de sables

International audienceThe characterization of sands detailed in this paper has been performed in order to support the in-flight radiometric performance assessment of space-borne optical sensors over so-called Pseudo-Invariant Calibration Sites (PICS). Although the physical properties of PICS surface are fairly stable in time, the signal measured from space varies with the illumination and the viewing geometries. Thus there is a need to characterize the spectro-directional properties of PICS. This can be done, at a broad scale, thanks to multi-spectral multi-directional space-borne sensors such as the POLDER instrument (with old data). However, interpolating or extrapolating the spectro-directional reflectances measured from space to spectral bands of another sensor is not straightforward. The hyperspectral characterization of sand samples collected within or nearby PICS can contribute to a solution. In this context, a set of 31 sand samples was compiled. The BiConical Reflectance Factor (BCRF) was measured between 0.4 and 2.5 µm, over a quarter hemisphere when the amount of sand in the sample was large enough and for only a single fixed angular configuration for small samples. These optical measurements were complemented by grain size distribution measurements and mineralogical analysis and compiled together with previously published measurements in the so-called PICSAND database, freely available on line.La caractérisation des sables détaillée dans cet article a été faite en soutien à l'estimation en vol des performances radiométriques des capteurs optiques spatiaux à partir des sites appelés PICS pour Pseudo-Invariant Calibration Sites. Bien que les propriétés physiques des PICS soient relativement stables dans le temps, le signal mesuré depuis l'espace varie en fonction des géométries d'illumination et d'observation. De ce fait, il est nécessaire de caractériser les propriétés spectro-directionnelles des PICS. Ceci peut être fait, à une grande échelle, à partir de capteurs spatiaux multi-spectraux et multi-directionnels tels que le capteur POLDER (avec des données anciennes). Cependant, l'interpolation ou l'extrapolation des réflectances spectro-directionnelles obtenues depuis l'espace aux bandes spectrales d'un autre capteur est délicate. La caractérisation hyperspectrale d'échantillons de sable issus de PICS ou de leur voisinage peut participer à une solution. Dans ce contexte, 31 échantillons de sable ont été collectés. Le Facteur de Reflectance BiConique (BCRF) a été mesuré entre 0,4 et 2,5 µm, pour une demi-hémisphère lorsque la quantité de sable était suffisante, et pour une seule géométrie pour les échantillons plus petits. Ces mesures optiques ont été complétées par des mesures de distribution en taille et par une analyse minéralogique, et mises dans une base de données appelée PICSAND avec d'autres mesures publiées dans la littérature. Cette base de donnée est en libre accès en ligne

Differentiation of human induced pluripotent stem cells towards notochordal-like cells: the role of tissue source

INTRODUCTION: Notochordal cells (NCs) are linked to a healthy intervertebral disc (IVD), and they are considered an exciting target for cell-based therapy. However, NCs are scarcely available as they are lost early in life, and attempts at in vivoexpansion have failed because NCs lose their specific phenotype. The production of Notochordal-like cells (NLCs) from human induced pluripotent stem cells (iPSCs) is a viable alternative. However, current attempts have been challenged by the low differentiation efficiency into the NC lineage. Therefore, the aim of this study was to build on the tissue-specific epigenetic memory of hiPSCs derived from IVD progenitor cells (TIE2+-cells) to improve hiPSC differentiation towards mature, matrix-producing NLCs. METHODS: hiPSCs were generated from TIE2⁺ cells of three adult donors. As a comparison, donormatched minimally invasive peripheral blood mononuclear (PBM) cell-derived iPSCs were used. Firstly, the iPSCs were differentiated into mesendodermal progenitors by Wnt pathway activation (N2B27 medium + 3µM CHIR99021)¹. Thereafter, the cells were further driven towards the NClineage by transfection with synthetic NOTO mRNA¹ and further matured using a 3D pellet culture in discogenic medium containing 10ng/mL TGF-β1. Read-out parameters included cell morphology, gene and protein expression and matrix deposition. RESULTS: Both TIE2⁺ and PBM cell-derived hiPSC showed successful differentiation towards mesendodermal progenitor cells following Wnt activation on day 2, indicated by the cells moving out of the colonies after CHIR stimulation. Accordingly, a decreased gene expression of pluripotency markers (OCT4, SOX2, NANOG), and upregulation of Wnt-target genes (LEF1, NODAL) and mesendodermal markers (TBXT, FOXA2, TBX6) was observed compared to mTESR1 controls. This was confirmed by immuno-stains for FOXA2 and TBXT. At day 3, we confirmed a 9-fold increase in NOTO mRNA levels after transfection in all donor lines. At day 28, the appearance of vacuolated NLCs was observed in both TIE2⁺ and PBM cell-derived pellet cultures confirming successful commitment towards the NC-lineage. Interestingly, while DMMB-assay detected GAG deposition in both lines, a significant increase in GAG content was seen in the TIE2⁺ cell-derived pellets. DISCUSSION & CONCLUSIONS: Tissue-specific TIE2⁺ cell-derived iPSCs may allow for an improved iPSNLC differentiation efficiency, indicated by the increased potency for deposition of GAG-rich matrix. Detailed analysis of the phenotypic markers and matrix deposited at the end of the 28 day maturation is ongoing to further document the phenotype of these iPS-NLCs. Delineating which epigenetic features are retained after reprogramming of these two cell lines, could shed light on the differences in their differentiation capacity. REFERENCES: ¹Colombier et al., 202