112 research outputs found
Independent Interactions of Phosphorylated β-Catenin with E-Cadherin at Cell-Cell Contacts and APC at Cell Protrusions
BACKGROUND: The APC tumour suppressor functions in several cellular processes including the regulation of β-catenin in Wnt signalling and in cell adhesion and migration. FINDINGS: In this study, we establish that in epithelial cells N-terminally phosphorylated β-catenin specifically localises to several subcellular sites including cell-cell contacts and the ends of cell protrusions. N-terminally phosphorylated β-catenin associates with E-cadherin at adherens junctions and with APC in cell protrusions. We isolated APC-rich protrusions from stimulated cells and detected β-catenin, GSK3β and CK1α, but not axin. The APC/phospho-β-catenin complex in cell protrusions appears to be distinct from the APC/axin/β-catenin destruction complex. GSK3β phosphorylates the APC-associated population of β-catenin, but not the cell junction population. β-catenin associated with APC is rapidly phosphorylated and dephosphorylated. HGF and wound-induced cell migration promote the localised accumulation of APC and phosphorylated β-catenin at the leading edge of migrating cells. APC siRNA and analysis of colon cancer cell lines show that functional APC is required for localised phospho-β-catenin accumulation in cell protrusions. CONCLUSIONS: We conclude that N-terminal phosphorylation of β-catenin does not necessarily lead to its degradation but instead marks distinct functions, such as cell migration and/or adhesion processes. Localised regulation of APC-phospho-β-catenin complexes may contribute to the tumour suppressor activity of APC
Cross sections and double-helicity asymmetries of midrapidity inclusive charged hadrons in p+p collisions at sqrt(s)=62.4 GeV
Unpolarized cross sections and double-helicity asymmetries of
single-inclusive positive and negative charged hadrons at midrapidity from p+p
collisions at sqrt(s)=62.4 GeV are presented. The PHENIX measurements for 1.0 <
p_T < 4.5 GeV/c are consistent with perturbative QCD calculations at
next-to-leading order in the strong coupling constant, alpha_s. Resummed pQCD
calculations including terms with next-to-leading-log accuracy, yielding
reduced theoretical uncertainties, also agree with the data. The
double-helicity asymmetry, sensitive at leading order to the gluon polarization
in a momentum-fraction range of 0.05 ~< x_gluon ~< 0.2, is consistent with
recent global parameterizations disfavoring large gluon polarization.Comment: PHENIX Collaboration. 447 authors, 12 pages, 5 figures, 5 tables.
Submitted to Physical Review
Inclusive cross section and single-transverse-spin asymmetry for very forward neutron production in polarized p+p collisions at sqrt(s)=200 GeV
The energy dependence of the single-transverse-spin asymmetry, A_N, and the
cross section for neutron production at very forward angles were measured in
the PHENIX experiment at RHIC for polarized p+p collisions at sqrt(s)=200 GeV.
The neutrons were observed in forward detectors covering an angular range of up
to 2.2 mrad. We report results for neutrons with momentum fraction of x_F=0.45
to 1.0. The energy dependence of the measured cross sections were consistent
with x_F scaling, compared to measurements by an ISR experiment which measured
neutron production in unpolarized p+p collisions at sqrt(s)=30.6--62.7 GeV. The
cross sections for large x_F neutron production for p+p collisions, as well as
those in e+p collisions measured at HERA, are described by a pion exchange
mechanism. The observed forward neutron asymmetries were large, reaching
A_N=-0.08+/-0.02 for x_F=0.8; the measured backward asymmetries, for negative
x_F, were consistent with zero. The observed asymmetry for forward neutron
production is discussed within the pion exchange framework, with interference
between the spin-flip amplitude due to the pion exchange and nonflip amplitudes
from all Reggeon exchanges. Within the pion exchange description, the measured
neutron asymmetry is sensitive to the contribution of other Reggeon exchanges
even for small amplitudes.Comment: 383 authors, 16 pages, 18 figures, 6 tables. Submitted to Phys. Rev.
D. Plain text data tables for the points plotted in figures for this and
previous PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Inclusive cross section and double helicity asymmetry for pi^0 production in p+p collisions at sqrt(s) = 62.4 GeV
The PHENIX experiment presents results from the RHIC 2006 run with polarized
proton collisions at sqrt(s) = 62.4 GeV for inclusive pi^0 production at
mid-rapidity. Unpolarized cross section results are measured for transverse
momenta p_T = 0.5 to 7 GeV/c. Next-to-leading order perturbative quantum
chromodynamics calculations are compared with the data, and while the
calculations are consistent with the measurements, next-to-leading logarithmic
corrections improve the agreement. Double helicity asymmetries A_LL are
presented for p_T = 1 to 4 GeV/c and probe the higher range of Bjorken_x of the
gluon (x_g) with better statistical precision than our previous measurements at
sqrt(s)=200 GeV. These measurements are sensitive to the gluon polarization in
the proton for 0.06 < x_g < 0.4.Comment: 387 authors from 63 institutions, 10 pages, 6 figures, 1 table.
Submitted to Physical Review D. Plain text data tables for the points plotted
in figures for this and previous PHENIX publications are (or will be)
publicly available at http://www.phenix.bnl.gov/papers.htm
Cold Nuclear Matter Effects on J/psi Yields as a Function of Rapidity and Nuclear Geometry in Deuteron-Gold Collisions at sqrt(s_NN) = 200 GeV
We present measurements of J/psi yields in d+Au collisions at sqrt(s_NN) =
200 GeV recorded by the PHENIX experiment and compare with yields in p+p
collisions at the same energy per nucleon-nucleon collision. The measurements
cover a large kinematic range in J/psi rapidity (-2.2 < y < 2.4) with high
statistical precision and are compared with two theoretical models: one with
nuclear shadowing combined with final state breakup and one with coherent gluon
saturation effects. To remove model dependent systematic uncertainties we also
compare the data to a simple geometric model. We find that calculations where
the nuclear modification is linear or exponential in the density weighted
longitudinal thickness are difficult to reconcile with the forward rapidity
data.Comment: 449 authors from 66 institutions, 6 pages, 3 figures. Submitted to
Physical Review Letters. Plain text data tables for the points plotted in
figures for this and previous PHENIX publications are (or will be) publicly
available at http://www.phenix.bnl.gov/papers.htm
Upsilon (1S+2S+3S) production in d+Au and p+p collisions at sqrt(s_NN)=200 GeV and cold-nuclear matter effects
The three Upsilon states, Upsilon(1S+2S+3S), are measured in d+Au and p+p
collisions at sqrt(s_NN)=200 GeV and rapidities 1.2<|y|<2.2 by the PHENIX
experiment at the Relativistic Heavy-Ion Collider. Cross sections for the
inclusive Upsilon(1S+2S+3S) production are obtained. The inclusive yields per
binary collision for d+Au collisions relative to those in p+p collisions
(R_dAu) are found to be 0.62 +/- 0.26 (stat) +/- 0.13 (syst) in the gold-going
direction and 0.91 +/- 0.33 (stat) +/- 0.16 (syst) in the deuteron-going
direction. The measured results are compared to a nuclear-shadowing model,
EPS09 [JHEP 04, 065 (2009)], combined with a final-state breakup cross section,
sigma_br, and compared to lower energy p+A results. We also compare the results
to the PHENIX J/psi results [Phys. Rev. Lett. 107, 142301 (2011)]. The rapidity
dependence of the observed Upsilon suppression is consistent with lower energy
p+A measurements.Comment: 495 authors, 11 pages, 9 figures, 5 tables. Submitted to Phys. Rev.
C. Plain text data tables for the points plotted in figures for this and
previous PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Medium modification of jet fragmentation in Au+Au collisions at sqrt(s_NN)=200 GeV measured in direct photon-hadron correlations
The jet fragmentation function is measured with direct photon-hadron
correlations in p+p and Au+Au collisions at sqrt(s_NN)=200 GeV. The p_T of the
photon is an excellent approximation to the initial p_T of the jet and the
ratio z_T=p_T^h/p_T^\gamma is used as a proxy for the jet fragmentation
function. A statistical subtraction is used to extract the direct photon-hadron
yields in Au+Au collisions while a photon isolation cut is applied in p+p. I_
AA, the ratio of jet fragment yield in Au+Au to that in p+p, indicates
modification of the jet fragmentation function. Suppression, most likely due to
energy loss in the medium, is seen at high z_T. The fragment yield at low z_T
is enhanced at large angles. Such a trend is expected from redistribution of
the lost energy into increased production of low-momentum particles.Comment: 562 authors, 70 insitutions, 8 pages, and 3 figures. Submitted to
Phys. Rev. Lett. v2 has minor changes to improve clarity. Plain text data
tables for the points plotted in figures for this and previous PHENIX
publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Measurements of elliptic and triangular flow in high-multiplicity HeAu collisions at GeV
We present the first measurement of elliptic () and triangular ()
flow in high-multiplicity HeAu collisions at
GeV. Two-particle correlations, where the particles have a large separation in
pseudorapidity, are compared in HeAu and in collisions and
indicate that collective effects dominate the second and third Fourier
components for the correlations observed in the HeAu system. The
collective behavior is quantified in terms of elliptic and triangular
anisotropy coefficients measured with respect to their corresponding
event planes. The values are comparable to those previously measured in
Au collisions at the same nucleon-nucleon center-of-mass energy.
Comparison with various theoretical predictions are made, including to models
where the hot spots created by the impact of the three He nucleons on the
Au nucleus expand hydrodynamically to generate the triangular flow. The
agreement of these models with data may indicate the formation of low-viscosity
quark-gluon plasma even in these small collision systems.Comment: 630 authors, 9 pages, 4 figures, 2 tables. v2 is the version accepted
for publication by Physical Review Letters. Plain text data tables for the
points plotted in figures for this and previous PHENIX publications are (or
will be) publicly available at http://www.phenix.bnl.gov/papers.htm
Identified charged hadron production in p+p collisions at sqrt(s)=200 and 62.4 GeV
Transverse momentum distributions and yields for , ,
and in collisions at =200 and 62.4 GeV at midrapidity
are measured by the PHENIX experiment at the Relativistic Heavy Ion Collider
(RHIC). These data provide important baseline spectra for comparisons with
identified particle spectra in heavy ion collisions at RHIC. We present the
inverse slope parameter , mean transverse momentum and
yield per unit rapidity at each energy, and compare them to other
measurements at different in and collisions. We
also present the scaling properties such as scaling, scaling on the
spectra between different energies. To discuss the mechanism of the
particle production in collisions, the measured spectra are compared to
next-to-leading-order or next-to-leading-logarithmic perturbative quantum
chromodynamics calculations.Comment: 431 authors from 62 institutions, 32 pages, 23 figures, and 18
tables. Submitted to Physical Review C. Plain text data tables for the points
plotted in figures for this and previous PHENIX publications are (or will be)
publicly available at http://www.phenix.bnl.gov/papers.htm
Direct photon production in d+Au collisions at sqrt(s_NN)=200 GeV
Direct photons have been measured in sqrt(s_NN)=200 GeV d+Au collisions at
midrapidity. A wide p_T range is covered by measurements of nearly-real virtual
photons (1<p_T<6 GeV/c) and real photons (5<p_T<16 GeV/c). The invariant yield
of the direct photons in d+Au collisions over the scaled p+p cross section is
consistent with unity. Theoretical calculations assuming standard cold nuclear
matter effects describe the data well for the entire p_T range. This indicates
that the large enhancement of direct photons observed in Au+Au collisions for
1.0<p_T<2.5 GeV/c is due to a source other than the initial-state nuclear
effects.Comment: 547 authors, 7 pages, 4 figures. Submitted to Phys. Rev. Lett.. Plain
text data tables for the points plotted in figures for this and previous
PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
- …