Poor sleep quality and progression of gait impairment in an incident Parkinson’s disease cohort

Abnormal sleep may associate with cognitive decline in Parkinson's disease (PD). Furthermore, sleep dysfunction may associate with worse motor outcome. We hypothesised that PD patients with poor quality sleep would have greater progression in gait dysfunction, due to structural and functional overlap in networks subserving sleep and gait regulation. 12 PD patients and 12 age-matched controls completed longitudinal follow-up over 36 months. Poor sleep efficiency and greater sleep fragmentation correlated significantly with progression of step-width variability, a gait characteristic mediated by postural control, providing evidence that poor sleep in PD is associated with a more rapid deterioration in gait

Amnion formation in the mouse embryo: the single amniochorionic fold model

<p>Abstract</p> <p>Background</p> <p>Despite the detailed knowledge obtained over the last decade on the molecular regulation of gastrulation in amniotes, the process of amnion development has been poorly described and illustrated in mice, and conflicting descriptions exist. Understanding the morphogenesis and development not only of the early mouse embryo, but also of its extraembryonic tissues, is crucial for correctly interpreting fate-mapping data and mouse mutants with gastrulation defects. Moreover, the recent isolation from amnion of cells with stem cell features further argues for a better understanding of the process of amnion formation. Here, we revisit the highly dynamic process of amnion formation in the mouse. Amnion development starts early during gastrulation and is intimately related to the formation of the exocoelom and the expansion of the amniotic fold. The authoritative description involves the fusion of two amniotic folds, a big posterior and a smaller anterior fold. We challenged this 'two amniotic folds' model by performing detailed histomorphological analyses of dissected, staged embryos and 3D reconstructions using historical sections.</p> <p>Results</p> <p>A posterior fold of extraembryonic ectoderm and associated epiblast is formed early during gastrulation by accumulation of extraembryonic mesoderm posterior to the primitive streak. Previously called the "posterior amniotic fold", we rename it the "amniochorionic fold" (ACF) because it forms both amnion and chorion. Exocoelom formation within the ACF seems not to involve apoptosis within the mesoderm. The ACF and exocoelom expand without disrupting the anterior junction of epiblast, extraembryonic ectoderm and visceral endoderm. No separate anterior fold is formed; its absence was confirmed in 3D reconstructions. Amnion and chorion closure is eccentric, close to the anterior margin of the egg cylinder: we name it the "anterior separation point".</p> <p>Conclusions</p> <p>Here, we reconcile previous descriptions of amnion formation and provide new nomenclature, as well as an animation, that clarify and emphasize the arrangement of the tissues that contribute to amnion development and the dynamics of the process. According to our data, the amnion and the chorion are formed by a single amniochorionic fold initiated posteriorly. Finally, we give an overview on mutant mouse models with impaired amnion development.</p

The development of the amnion in mice and other amniotes

The amnion is an extraembryonic tissue that evolutionarily allowed embryos of all amniotes to develop in a transient and local aquatic environment. Despite the importance of this tissue, very little is known about its formation and its molecular characteristics. In this review, we have compared the basic organization of the extraembryonic membranes in amniotes and describe the two types of amniogenesis, folding and cavitation. We then zoom in on the atypical development of the amnion in mice that occurs via the formation of a single posterior amniochorionic fold. Moreover, we consolidate lineage tracing data to better understand the spatial and temporal origin of the progenitors of amniotic ectoderm, and visualize the behaviour of their descendants in the extraembryonic-embryonic junctional region. This analysis provides new insight on amnion development and expansion. Finally, using an online-available dataset of single-cell transcriptomics during the gastrulation period in mice, we provide bioinformatic analysis of the molecular signature of amniotic ectoderm and amniotic mesoderm. The amnion is a tissue with unique biomechanical properties that deserves to be better understood. This article is part of the theme issue 'Extraembryonic tissues: exploring concepts, definitions and functions across the animal kingdom'

Color afterimages in autistic adults

It has been suggested that attenuated adaptation to visual stimuli in autism is the result of atypical perceptual priors (e.g., Pellicano and Burr in Trends Cogn Sci 16(10):504–510, 2012. doi:10.​1016/​j.​tics.​2012.​08.​009). This study investigated adaptation to color in autistic adults, measuring both strength of afterimage and the influence of top-down knowledge. We found no difference in color afterimage strength between autistic and typical adults. Effects of top-down knowledge on afterimage intensity shown by Lupyan (Acta Psychol 161:117–130, 2015. doi:10.​1016/​j.​actpsy.​2015.​08.​006) were not replicated for either group. This study finds intact color adaptation in autistic adults. This is in contrast to findings of attenuated adaptation to faces and numerosity in autistic children. Future research should investigate the possibility of developmental differences in adaptation and further examine top-down effects on adaptation

BHPR research: qualitative1. Complex reasoning determines patients' perception of outcome following foot surgery in rheumatoid arhtritis

Background: Foot surgery is common in patients with RA but research into surgical outcomes is limited and conceptually flawed as current outcome measures lack face validity: to date no one has asked patients what is important to them. This study aimed to determine which factors are important to patients when evaluating the success of foot surgery in RA Methods: Semi structured interviews of RA patients who had undergone foot surgery were conducted and transcribed verbatim. Thematic analysis of interviews was conducted to explore issues that were important to patients. Results: 11 RA patients (9 ♂, mean age 59, dis dur = 22yrs, mean of 3 yrs post op) with mixed experiences of foot surgery were interviewed. Patients interpreted outcome in respect to a multitude of factors, frequently positive change in one aspect contrasted with negative opinions about another. Overall, four major themes emerged. Function: Functional ability & participation in valued activities were very important to patients. Walking ability was a key concern but patients interpreted levels of activity in light of other aspects of their disease, reflecting on change in functional ability more than overall level. Positive feelings of improved mobility were often moderated by negative self perception ("I mean, I still walk like a waddling duck”). Appearance: Appearance was important to almost all patients but perhaps the most complex theme of all. Physical appearance, foot shape, and footwear were closely interlinked, yet patients saw these as distinct separate concepts. Patients need to legitimize these feelings was clear and they frequently entered into a defensive repertoire ("it's not cosmetic surgery; it's something that's more important than that, you know?”). Clinician opinion: Surgeons' post operative evaluation of the procedure was very influential. The impact of this appraisal continued to affect patients' lasting impression irrespective of how the outcome compared to their initial goals ("when he'd done it ... he said that hasn't worked as good as he'd wanted to ... but the pain has gone”). Pain: Whilst pain was important to almost all patients, it appeared to be less important than the other themes. Pain was predominately raised when it influenced other themes, such as function; many still felt the need to legitimize their foot pain in order for health professionals to take it seriously ("in the end I went to my GP because it had happened a few times and I went to an orthopaedic surgeon who was quite dismissive of it, it was like what are you complaining about”). Conclusions: Patients interpret the outcome of foot surgery using a multitude of interrelated factors, particularly functional ability, appearance and surgeons' appraisal of the procedure. While pain was often noted, this appeared less important than other factors in the overall outcome of the surgery. Future research into foot surgery should incorporate the complexity of how patients determine their outcome Disclosure statement: All authors have declared no conflicts of interes

Hydrological conditions explain variation in wood density in riparian plants of south-eastern Australia

1: Wood density is a key plant functional trait which integrates the trade-offs characteristic to riparian plant ecological strategies. Although high-density wood is costly to construct, it confers mechanical stiffness to stems, increasing a plant's capacity to withstand flooding, and also enables increased tolerance to water stress. For riparian plants, fluctuations in soil moisture driven by surface hydrology should therefore be an important driver of variation in wood density. 2: We asked the following questions in the study: (1) Does wood density increase with increasing frequency and magnitude of flood disturbance? (2) Does wood density increase with increasing unpredictability of water availability in the riparian zone? (3) Does dispersion of wood density peak at intermediate levels of hydrological disturbance? 3: We surveyed wood density of dominant species at 15 riparian sites along flow-gauged rivers across south-eastern Australia. Due to the broad range of hydrological variability associated with Australian river systems, this set of sites functions as a useful model for assessing the response of riparian plants to changing hydrological conditions. 4: We found wood density varied strongly along a single axis of hydrological variability. This axis integrates flood intensity and frequency with metrics of hydrological unpredictability and can be conceptualized as a gradient of environmental harshness, with higher wood density associated with harsher conditions. 5: Synthesis. Our study highlights the importance of hydrological conditions, particularly disturbance and environmental unpredictability, as determinants of ecological strategy in riparian plants. Large, rare flood events in particular appear to favour higher wood density strategies. This is likely to have significant ecological consequences for riparian plant communities in a south-east Australian context, as well as in other regions where increasing climatic variability and frequency of extreme events are hallmarks of climate change predictions.12 page(s

Data from: Hydrological conditions explain wood density in riparian plants of south-eastern Australia

1. Wood density is a key plant functional trait which integrates the trade-offs characteristic to riparian plant ecological strategies. Although high-density wood is costly to construct, it confers mechanical stiffness to stems, increasing a plant's capacity to withstand flooding, and also enables increased tolerance to water stress. For riparian plants, fluctuations in soil moisture driven by surface hydrology should therefore be an important driver of variation in wood density. 2. We asked the following questions in the study: (1) Does wood density increase with increasing frequency and magnitude of flood disturbance? (2) Does wood density increase with increasing unpredictability of water availability in the riparian zone? (3) Does dispersion of wood density peak at intermediate levels of hydrological disturbance? 3. We surveyed wood density of dominant species at 15 riparian sites along flow-gauged rivers across south-eastern Australia. Due to the broad range of hydrological variability associated with Australian river systems, this set of sites functions as a useful model for assessing the response of riparian plants to changing hydrological conditions. 4. We found wood density varied strongly along a single axis of hydrological variability. This axis integrates flood intensity and frequency with metrics of hydrological unpredictability and can be conceptualized as a gradient of environmental harshness, with higher wood density associated with harsher conditions. 5. Synthesis. Our study highlights the importance of hydrological conditions, particularly disturbance and environmental unpredictability, as determinants of ecological strategy in riparian plants. Large, rare flood events in particular appear to favour higher wood density strategies. This is likely to have significant ecological consequences for riparian plant communities in a south-east Australian context, as well as in other regions where increasing climatic variability and frequency of extreme events are hallmarks of climate change predictions

Effects of biosolids amendments on American Kestrel (Falco sparverius) nest-site selection and diet

Fertilization is a common practice, as it improves plant yields. In many areas, biosolids (remains from treated wastewater) are used instead of chemical fertilizers, due to multi-year nutrient release and positive changes in soils and water retention. Little is known about whether biosolids affect animal consumers. Here, we examine diets and nesting sites of American Kestrels (Falco sparverius L. 1758) in British Columbia, Canada. Our major study area was a large cattle ranch that has been grazed for over a century, and where biosolids were applied in the previous 1-3 years. Kestrels selected nests in areas with higher Northern Flicker (Colaptes auratus L. 1758) nest density and with a higher proportion of pastures with biosolids applications, but with less forest. Kestrels were observed more often on biosolids-amended pastures than in untreated areas. Kestrels primarily ate voles (Microtus spp.), but also consumed grasshoppers (Orthoptera) late in the breeding season when grasshoppers were abundant. Kestrel diets were slightly more varied on a nearby control area than on the ranch. Our results suggest that biosolids applications improve prey availability for kestrels, thus potentially acting as a restoration tool in areas with degraded habitats or where kestrels have declined.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Heterogeneous flows foster heterogeneous assemblages : relationships between functional diversity and hydrological heterogeneity in riparian plant communities

Riparian ecosystems are biophysically complex and highly diverse taxonomically, structurally and functionally. While many environmental factors determine the structure and function of riparian vegetation communities, hydrology is thought to be the 'master variable'. Flooding and variability in water availability are known to be key drivers of taxonomic diversity, but their influence on the functional trait diversity of riparian vegetation communities remains largely unexplored. We collected data on species abundance, quantitative plant functional traits and hydrology from 15 sites distributed across south-eastern Australia to address the following questions: (a) Is functional trait diversity related to the frequency and magnitude of flooding disturbance? (b) Is functional trait diversity related to variability in seasonal water availability within the riparian zone? We confirm that metrics describing both flooding disturbance and patterns of water availability exhibit strong relationships with functional trait diversity in riparian vegetation communities of south-eastern Australia. Our key finding is that functional trait diversity in these systems tends to be positively associated with variability in hydrological conditions and the intensity of rare, high-magnitude flooding events, rather than average patterns of flow. Our study highlights the importance of extreme flooding events and temporal patterns of water availability as determinants of diversity in riparian vegetation communities. These relationships may have significant consequences for plant communities experiencing alterations to hydrology caused by anthropogenic flow modification and the changing climate.18 page(s