Localization of axonally transported 125I-wheat germ agglutinin beneath the plasma membrane of chick retinal ganglion cells.

The distribution of 125I-wheat germ agglutinin (WGA) transported by axons of chick retinal ganglion cells to layer d of the optic tectum was studied by electron microscopic autoradiography. We found that 52% of the radioactivity was located in axons and axon terminals in the contralateral optic tectum 22 h after intravitreal injection of affinity-purified 125I-WGA. Axons comprised 43% of the volume of layer d. Dendrites, glial cells, and neuron cell bodies contained 20%, 17%, and 3% of the label, whereas these structures comprised 24%, 21%, and 2% of the tissue volume, respectively. We also measured the distances between the autoradiographic silver grains and the plasma membranes of these profiles, and compared observed distributions of grains to theoretical distributions computed for band-shaped sources at various distances from the plasma membranes. This analysis revealed that the radioactive source within axons was distributed in a band of cytoplasm extending in from the plasma membrane a distance of 63 nm. Because WGA is known to bind to specific membrane glycoconjugates, we infer that at least some glycoconjugates may be concentrated within an annular region of cytoplasm just beneath the axonal plasma membrane after axoplasmic transport from the neuron cell body

KINETICS OF ROD OUTER SEGMENT RENEWAL IN THE DEVELOPING MOUSE RETINA

The kinetics of rod outer segment renewal in the developing retina have been investigated in C57BL/6J mice. Litters of mice were injected with [3H]amino acids at various ages and killed at progressively later time intervals. Plastic 1.5 µm sections of retina were studied by light microscope autoradiography. The rate of outer segment disk synthesis, as judged by labeled disk displacement away from the site of synthesis, is slightly greater than the adult level at 11–13 days of age; it rises to more than 1.6 times the adult rate between days 13 and 17, after which it falls to the adult level at 21–25 days. The rate of disk disposal, as measured by labeled disk movement toward the site of disposal, is less than 15% of the adult level at 11–13 days of age; it rises sharply to almost 70% of the adult level by days 13–15 and then more gradually approaches the adult rate. The net difference in rates of synthesis and disposal accounts for the rapid elongation of rod outer segments in the mouse between days 11 and 17 and the subsequent, more gradual elongation to the adult equilibrium length reached between days 19 and 25. The changing rate of outer segment disk synthesis characterizes the late stages of cytodifferentiation of the rod photoreceptor cells

Characterising the surface magnetic fields of T Tauri stars with high-resolution near-infrared spectroscopy

We aim to characterise the surface magnetic fields of a sample of 8 T Tauri stars from high-resolution near-IR spectroscopy. Some stars in our sample are known to be magnetic from previous spectroscopic or spectropolarimetric studies. Our goals are 1) to apply Zeeman broadening modelling to T Tauri stars with high-resolution data, 2) to expand the sample of stars with measured surface magnetic field strengths, 3) to investigate possible rotational or long-term magnetic variability by comparing spectral time series of given targets, and 4) to compare the magnetic field modulus tracing small-scale magnetic fields to those of large-scale magnetic fields derived by Stokes V Zeeman Doppler Imaging. We modelled the Zeeman broadening of magnetically sensitive spectral lines in the near-IR K-band from high-resolution spectra by using magnetic spectrum synthesis based on realistic model atmospheres and by using different descriptions of the surface magnetic field. We developped a Bayesian framework that selects the complexity of the magnetic field prescription based on the information contained in the data. We obtain individual magnetic field measurements for each star in our sample using four different models. We find that the Bayesian Model 4 performs best in the range of magnetic fields measured on the sample (from 1.5 kG to 4.4 kG). We do not detect a strong rotational variation of with a mean peak-to-peak variation of 0.3 kG. Our confidence intervals are of the same order of magnitude, which suggests that the Zeeman broadening is produced by a small-scale magnetic field homogeneously distributed over stellar surfaces. A comparison of our results with mean large-scale magnetic field measurements from Stokes V ZDI show different fractions of mean field strength being recovered, from 25-42% for relatively simple poloidal axisymmetric field topologies to 2-11% for more complex fields.Comment: 14 pages, 9 figures, accepted for publication in Astronomy and Astrophysic

Magnetic fields of intermediate mass T Tauri stars

Aims. In this paper, we aim to measure the strength of the surface magnetic fields for a sample of five intermediate mass T Tauri stars and one low mass T Tauri star from late-F to mid-K spectral types. While magnetic fields of T Tauri stars at the low mass range have been extensively characterized, our work complements previous studies towards the intermediate mass range; this complementary study is key to evaluate how magnetic fields evolve during the transition from a convective to a radiative core. Methods. We studied the Zeeman broadening of magnetically sensitive spectral lines in the H-band spectra obtained with the CRIRES high-resolution near-infrared spectrometer. These data are modelled using magnetic spectral synthesis and model atmospheres. Additional constraints on non-magnetic line broadening mechanisms are obtained from modelling molecular lines in the K band or atomic lines in the optical wavelength region. Results. We detect and measure mean surface magnetic fields for five of the six stars in our sample: CHXR 28, COUP 107, V2062 Oph, V1149 Sco, and Par 2441. Magnetic field strengths inferred from the most magnetically sensitive diagnostic line range from 0.8 to 1.8 kG. We also estimate a magnetic field strength of 1.9 kG for COUP 107 from an alternative diagnostic. The magnetic field on YLW 19 is the weakest in our sample and is marginally detected, with a strength of 0.8 kG. Conclusions. We populate an uncharted area of the pre-main-sequence HR diagram with mean magnetic field measurements from high-resolution near-infrared spectra. Our sample of intermediate mass T Tauri stars in general exhibits weaker magnetic fields than their lower mass counterparts. Our measurements will be used in combination with other spectropolarimetric studies of intermediate mass and lower mass T Tauri stars to provide input into pre-main-sequence stellar evolutionary models.Comment: 8 pages, 8 figures, accepted for publication in Astronomy and Astrophysic

From the creation of the Women’s Section of Falange (1934) to the electoral campaign in 1936: modalities of falangist women’s intervention in the public sphere

La creación de la Sección Femenina, aunque significa de facto que los falangistas aceptan cierta presencia de “sus” mujeres en la esfera pública, remite a la defensa de un modelo femenino anclado en el espacio privado. Sus actividades, concebidas como una extensión del papel doméstico de la mujer al ámbito público, son pues esencialmente sociales. En 1936, con la campaña electoral, parecen ampliarse a lo político.Este trabajo se interroga sobre el sentido y la realidad de esta aparente evolución así como sobre las contradicciones de la Sección Femenina, su dificultad en posicionarse entre la esfera pública y la privada. Estas contradicciones se hacen patentes en la figura de Rosario Pereda, jefe de la Sección Femenina de Valladolid y oradora reconocida y apreciada por sus camaradas masculinos.The founding of the Women’s Section of the Falange, despite signifying that the Falangists accept a certain presence of “their” women in the public sphere, defends a feminine model anchored in the private area. Its activities are essentially social, conceived as an extension of the domestic role of women into the public sphere. In 1936, however, with the electoral campaign, these roles seem to be extended to the political domain.The present study aims to analyse the meaning and the reality of this apparent evolution of the Women’s Section with its contradictions and its difficulty in positioning itself between the public and the private spheres. These contradictions become clear in the figure of Rosario Pereda, chief of the Women’s Section of Valladolid, who was a recognized orator esteemed by her masculine comrades