Péptidos catiónicos anfipáticos y su aplicación en vectores de transferencia génica

Referencia OEPM: P200001806.-- Fecha de solicitud: 20/07/2000.-- Titulares: Consejo Superior de Investigaciones Científicas (CSIC), Medplant Genetics, S.L. y Jesús Fominaya Gutiérrez.Péptidos catiónicos anfipáticos y su aplicación en vectores de transferencia génica. Estos péptidos comprenden unos restos de aminoácidos básicos, alifáticos y aromáticos, unos restos de aminoácidos básicos en los dos extremos del péptido y, preferentemente, un resto de un aminoácido aromático en la posición 3 de la secuencia del péptido. Estos péptidos tienen simultáneamente la capacidad de unir un polinucleótido y alterar membranas biológicas y son útiles para construir vectores no virales de transferencia génica, con una capacidad de condensación de material genético mejorada, y/o con una citotoxicidad reducida y/o con una eficiencia de transferencia de material genético mejorada. De aplicación en la transferencia génica con fines experimentales y terapéuticos.Peer reviewe

Proteómica, poderosa pero poco explorada ómica en la búsqueda y desarrollo de nuevos fármacos

Proteómica, poderosa pero poco explorada ómica en la búsqueda y desarrollo de nuevos fármacos

Analysis of the legalization of microenterprise in Popayán

Con este trabajo se pretende desarrollar el análisis de la legalización de la microempresa en Popayán, identificando y evaluando los puntos más críticos del problema con el fin de hacer sugerencias sobre aquellos aspectos que son prioritarios para un posible régimen de excepción. Así mismo, presentar algunas alternativas consagradas en la legislación vigente que favorezcan o en su defecto requieran modificaciones relativas a los límites de excepción, exoneración, o cualquier otra de las condiciones que contempla la Ley. Además proveer al microempresario de nuestra región de una guía que lo ilustre, paso a paso, y le indique los costos de los trámites de legalización exigidos en nuestro medio, ante la imposibilidad de eliminar en el corto plazo las condiciones que impiden que lo normativo encuentre aplicabilidad real para una transición paulatina de la microempresa hacia lo formal.Instituto Tecnológico de Estudios Superiores de Monterrey ITESMMaestríaThis work aims to develop the analysis of the legalization of microenterprises in Popayán, identifying and evaluating the most critical points of the problem in order to make suggestions on those aspects that are priority for a possible exception regime. Likewise, present some alternatives enshrined in the current legislation that favor or, failing that, require modifications regarding the limits of exception, exemption, or any other of the conditions contemplated by the Law. In addition, provide the micro-entrepreneur in our region with a guide that I illustrate it, step by step, and indicate the costs of the legalization procedures required in our environment, given the impossibility of eliminating in the short term the conditions that prevent the regulations from finding real applicability for a gradual transition of the microenterprise towards the formal.Modalidad Presencia

Preclinical Efficacy of Endoglin-Targeting Antibody–Drug Conjugates for the Treatment of Ewing Sarcoma

[EN] Endoglin (ENG; CD105) is a coreceptor of the TGFb family that is highly expressed in proliferating endothelial cells. Often coopted by cancer cells, ENG can lead to neo-angiogenesis and vasculogenic mimicry in aggressive malignancies. It exists both as a transmembrane cell surface protein, where it primarily interacts with TGFb, and as a soluble matricellular protein (sENG) when cleaved by matrix metal-loproteinase 14 (MMP14). High ENG expression has been associated with poor prognosis in Ewing sarcoma, an aggressive bone cancer that primarily occurs in adolescents and young adults. However, the therapeutic value of ENG targeting has not been fully explored in this disease. Experimental Design: We characterized the expression pattern of transmembrane ENG, sENG, and MMP14 in preclinical and clinical samples. Subsequently, the antineoplastic potential of two novel ENG-targeting monoclonal antibody–drug conjugates (ADC), OMTX503 and OMTX703, which differed only by their drug payload (nigrin-b A chain and cytolysin, respectively), was assessed in cell lines and preclinical animal models of Ewing sarcoma. Results: Both ADCs suppressed cell proliferation in proportion to the endogenous levels of ENG observed in vitro. Moreover, the ADCs significantly delayed tumor growth in Ewing sarcoma cell line–derived xenografts and patient-derived xenografts in a dose-dependent manner. Conclusions: Taken together, these studies demonstrate potent preclinical activity of first-in-class anti-ENG ADCs as a nascent strategy to eradicate Ewing sarcoma

Caracterización de la región 5' no codificante del virus del la sharka

Tesis doctoral inédita leida en la Universidad Autónoma de Madrid. Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 13-12-199

Preservation of 5'-end integrity of a potyvirus genomic RNA is not dependent on template specificity

Full-length in vitro transcripts of plum pox potyvirus (PPV) genomic RNA with mutations altering the number of 5'-terminal adenosine residues were able to infect Nicotiana clevelandii plants, whereas a mutant with a substitution of adenosine in position 2 by guanosine failed to infect. The genomic 5' end was template-independently repaired during in vivo RNA synthesis producing wild-type viral progeny. Putative models of replication initiation are discussed. (C) 2000 Academic Press.This work was supported by Grants BIO98-0769 from CICYT, 06G/ 021/96 from Comunidad de Madrid, and BIO4-CT96-0304 and BIO4- CT97-2300 from the Biotechnology Program of the European Union.Peer Reviewe

Expression of Epithelial and Mesenchymal Markers in Plasmatic Extracellular Vesicles as a Diagnostic Tool for Neoplastic Processes

Tumor-derived extracellular vesicles (TD-EVs) have active roles as cancer hallmark enablers. EVs RNA of epithelial and stromal cells carry information that facilitates the communication processes that contribute to oncological progression, so the objective of this work was to validate by RT-PCR the presence of epithelial (KRT19; CEA) and stromal (COL1A2; COL11A1) markers in RNA of plasmatic EVs in healthy and diverse-malignancy patients for the development of a non-invasive cancer diagnosis system using liquid biopsy. Ten asymptomatic controls and 20 cancer patients were included in the study, and results showed that the isolated plasmatic EVs by scanning transmission electron microscopy (STEM) andBiomedical Research Institute A Coruña nanoparticle tracking analysis (NTA) contained most exosome structures with also a considerable percentage of microvesicles. No differences were found in concentration and size distribution between the two cohorts of patients, but significant gene expression in epithelial and mesenchymal markers between healthy donors and patients with active oncological disease was shown. Results of quantitative RT-PCR are solid and reliable for KRT19, COL1A2, and COL11A1, so the analysis of RNA extracted from TD-EVs could be a correct approach to develop a diagnostic tool in oncological processes