An IMS-Learning Design Editor for a Higher Education Blended Learning Scenario

The IMS-Learning Design has been developed to support the creation of reusable and pedagogically neutral learning scenarios and content. Although it is especially suitable for eLearning, there is a lot of interest on using it in higher education blended learning scenarios. However there are some related key issues which must be managed such as cultural bias and the need for expensive human resources to design and develop specification compliant units of learning. They can be addressed by the design of ad-hoc editors supporting concrete learning design units of learning. We suggest some solutions to overcome these limitations, based on our experience designing the user interface of an IMS-LD compliant editor, GDUS+. We also explain our user centering approach, and give some conclusions about the benefits of using IMS-LD

Las parteras de Guadalajara (México) en el siglo XIX : el despojo de su arte

En este escrito examinamos la depreciación social de los saberes y prácticas que utilizan las parteras en su actividad laboral. El estudio se circunscribe a sucesos históricos que acontecen a lo largo del siglo XIX en la ciudad de Guadalajara, capital del estado de Jalisco, México. Aquí se muestra como el desplazamiento y subordinación de estas mujeres están asociados al mayor posicionamiento social de los médicos, quienes apoyados en el avance de la ciencia médica y en la autoridad que se otorga a los conocimientos provenientes de instituciones formales de enseñanza, empiezan a socavar el valor de los saberes empíricos y a sobreponer los conocimientos provenientes de los avances de la ciencia médica. A través de dos ejes se expone la manera como los médicos despojan y subordinan a las parteras al campo científico-laboral de la medicina: mediante un discurso degradante de los saberes empíricos y por medio de la institucionalización de cursos de capacitación que tienden a desconocer el saber hacer de estas mujeres y a sustituir sus habilidades con conocimientos derivados de la ciencia médica. El estudio se basa en información proveniente de archivos y de revistas de la época: del Archivo General de Guadalajara (AGG), del Archivo Histórico de la Universidad de Guadalajara (AHUG) y de la Hemeroteca de la Biblioteca Pública del estado de Jalisco (BPEJ)

Synthesis and characterization of o-fluorosulfinylaniline: A comparative vibrational study of fluorinated sulfinylaniline series

The synthesis of o-fluorosulfinylaniline is reported with the aim to complete the fluorinated sulfinylaniline series. The FT-IR and Raman spectra of o-fluorosulfinylaniline are recorded and the fundamental modes of its vibrational frequencies are assigned together with a tentative assignment of the NMR and mass spectra. Quantum chemical calculations on the optimized geometry predict in the liquid phase a planar structure with syn orientation of the –N[double bond, length as m-dash]S[double bond, length as m-dash]O moiety (syn of the S[double bond, length as m-dash]O double bond relative to the C–N single bond), which belongs to the CS symmetry group. The experimentally observed spectral data (FT-IR, Raman, 1H and 13C and GC-mass spectrometry) of the title compound are compared with those obtained theoretically from DFT calculations and the gauge including atomic orbital (GIAO) method. Vibrational frequencies were assigned with the aid of anharmonic variational methods (VSCF) obtaining a significantly better agreement with experiment than with simple harmonic oscillator methods. Moreover, stability of the molecule arising from hyperconjugative interactions and charge delocalization has been analyzed using natural bond orbital (NBO) analysis.Fil: Paez Jerez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Alonso de Armiño, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Robles, Norma Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentin

Citrus psorosis virus movement protein contains an aspartic protease required for autocleavage and the formation of tubule-like structures at plasmodesmata

Plant virus cell-to-cell movement is an essential step in viral infections. This process is facilitated by specific virus-encoded movement proteins (MPs), which manipulate the cell wall channels between neighboring cells known as plasmodesmata (PD). Citrus psorosis virus (CPsV) infection in sweet orange involves the formation of tubule-like structures within PD, suggesting that CPsV belongs to "tubuleforming" viruses that encode MPs able to assemble a hollow tubule extending between cells to allow virus movement. Consistent with this hypothesis, we show that the MP of CPsV (MPCPsV) indeed forms tubule-like structures at PD upon transient expression in Nicotiana benthamiana leaves. Tubule formation by MPCPsV depends on its cleavage capacity, mediated by a specific aspartic protease motif present in its primary sequence. A single amino acid mutation in this motif abolishes MPCPsV cleavage, alters the subcellular localization of the protein, and negatively affects its activity in facilitating virus movement. The amino-terminal 34-kDa cleavage product (34KCPsV), but not the 20-kDa fragment (20KCPsV), supports virus movement. Moreover, similar to tubule-forming MPs of other viruses, MPCPsV (and also the 34KCPsV cleavage product) can homooligomerize, interact with PD-located protein 1 (PDLP1), and assemble tubule-like structures at PD by a mechanism dependent on the secretory pathway. 20KCPsV retains the protease activity and is able to cleave a cleavage-deficient MPCPsV in trans. Altogether, these results demonstrate that CPsV movement depends on the autolytic cleavage of MPCPsV by an aspartic protease activity, which removes the 20KCPsV protease and thereby releases the 34KCPsV protein for PDLP1-dependent tubule formation at PD. IMPORTANCE Infection by citrus psorosis virus (CPsV) involves a self-cleaving aspartic protease activity within the viral movement protein (MP), which results in the production of two peptides, termed 34KCPsV and 20KCPsV, that carry the MP and viral protease activities, respectively. The underlying protease motif within the MP is also found in the MPs of other members of the Aspiviridae family, suggesting that protease-mediated protein processing represents a conserved mechanism of protein expression in this virus family. The results also demonstrate that CPsV and potentially other ophioviruses move by a tubule-guided mechanism. Although several viruses from different genera were shown to use this mechanism for cell-to-cell movement, our results also demonstrate that this mechanism is controlled by posttranslational protein cleavage. Moreover, given that tubule formation and virus movement could be inhibited by a mutation in the protease motif, targeting the protease activity for inactivation could represent an important approach for ophiovirus control.Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Peña, Eduardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Heinlein, Manfred. Université de Strasbourg; Francia. Centre National de la Recherche Scientifique; FranciaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin

Comparison Between Supervised and Unsupervised Classifications of Neuronal Cell Types: A Case Study

In the study of neural circuits, it becomes essential to discern the different neuronal cell types that build the circuit. Traditionally, neuronal cell types have been classified using qualitative descriptors. More recently, several attempts have been made to classify neurons quantitatively, using unsupervised clustering methods. While useful, these algorithms do not take advantage of previous information known to the investigator, which could improve the classification task. For neocortical GABAergic interneurons, the problem to discern among different cell types is particularly difficult and better methods are needed to perform objective classifications. Here we explore the use of supervised classification algorithms to classify neurons based on their morphological features, using a database of 128 pyramidal cells and 199 interneurons from mouse neocortex. To evaluate the performance of different algorithms we used, as a “benchmark,” the test to automatically distinguish between pyramidal cells and interneurons, defining “ground truth” by the presence or absence of an apical dendrite. We compared hierarchical clustering with a battery of different supervised classification algorithms, finding that supervised classifications outperformed hierarchical clustering. In addition, the selection of subsets of distinguishing features enhanced the classification accuracy for both sets of algorithms. The analysis of selected variables indicates that dendritic features were most useful to distinguish pyramidal cells from interneurons when compared with somatic and axonal morphological variables. We conclude that supervised classification algorithms are better matched to the general problem of distinguishing neuronal cell types when some information on these cell groups, in our case being pyramidal or interneuron, is known a priori. As a spin-off of this methodological study, we provide several methods to automatically distinguish neocortical pyramidal cells from interneurons, based on their morphologies

The nurse’s humanistic functioning in relation to the pain of the child patient with burns

El artículo tiene como objetivo reflejar sobre la actuación humanística de la enfermera en la intervención del dolor nociceptivo somático de los niños. Fue realizado un estudio cualitativo descriptivo direccionado y discutido de acuerdo con la fenomenología de Husserl, Se consideraron los aspectos ético-legales, fueron entrevistadas diez enfermeras expertas en área de quemados; los datos relevantes fueron seleccionados por la técnica colorimétrica, para después efectuar un análisis de contenido. Los resultados revelaron que la enfermera es una profesional que actúa afectivamente en situaciones de angustia, tristeza, protegiendo de manera solidaria al paciente infantil, también que las terapias no farmacológicas como la ludoterapia, risoterapia, musicoterapia, ejercicios de relajación, respiración y apoyo psicológico coadyuvan en el combate del dolor. Al concluir se reflejó que la intervención de la enfermera es altamente humanística, y se revela que el dolor requiere ser tratado por profesionales con amplia sensibilidad para brindar cuidado altamente humano.This article aims to reflect on the humanistic functioning of the nurse in the interventions with nociceptive, somatic pain in children. To this end a qualitative descriptive study was undertaken, directed and discussed in accordance with Husserlian phenomenology. Ethical-legal aspects were taken into consideration, interviews being held with 10 nurses who are specialists in the area of burns. The relevant data were selected through the colorimetric technique, so as to later undertake content analysis. The results revealed the nurse to be a professional who acts affectively in situations of distress and sadness, protecting the child patient empathetically. In addition, it is possible to perceive that non-pharmacological therapies, such as play therapy, laughter therapy, music therapy, relaxation exercises, breathing exercises, and psychological support are coadjuvant in combating pain. At the end of the study, it is reflected that the nurse’s intervention is highly humanistic, and it is shown that pain must be treated by professionals with great sensitivity, so as to promote highly humane car

VOCES DE LAS ENFERMERAS AL PERCIBIR EL DOLOR DEL PACIENTE INFANTIL QUEMADO

El objetivo del estudio fue revelar la percepción de la enfermera frente al dolor nociceptivo del paciente infantil que sufre quemaduras. Desarrollado en un hospital pediátrico del Distrito Federal, México. Investigación cualitativa, de tipo descriptivo. Para la recolección se aplicó una guía de entrevista semiestructurada a diez enfermeras del área de quemados, siendo los datos analizados mediante análisis de contenido. Como resultados se obtuvieron tres categorías. Se utilizó como referencial teórico la fenomenología. Los resultados muestran las vivencias de las enfermeras, la expresión, del mecanismos de conducta auto-defensiva, en la percepción de sus intervenciones, se destaca la carga humanística que tienen las emociones, pues el hecho de manifestarlas implica un gasto extra de fuerza y energía, resultando que a mayor intensidad de la emoción a la que se enfrentan, más es el trabajo emocional que realizan. En conclusión el sujeto aprende las vivencias del yo por medio de la experiencia

Characterizing Mineral Crystallographic Preferred Orientations (cpos) Along The Eastern North American Margin In The Southern Appalachians: Implications For Middle And Lower Crustal Seismic Anisotropy

The Southern Appalachians Mountains have a complex and distinctive tectonic history, characterized by structural deformation extending from late Precambrian throughout the Paleozoic. The Southern Appalachians, known as the Blue Ridge of western North Carolina and eastern Tennessee, is mainly composed of schists and gneisses, granitic and ultramafic intrusives, as well as a sedimentary portion. The main tool for understanding the composition and structure of the deeper parts of the crust that cannot be directly sampled by seismology. Seismic anisotropy is the directional dependence of seismic velocity, and it is an invaluable tool for interpreting the development of sub-surficial dynamics. Seismic anisotropy can have a number of controlling parameters including shear strain, mineral grain aspect ratios, and aligned cracks, and crystallographic preferred orientations. If these parameters are known, we can use anisotropy to constrain lower and middle crustal composition. In an effort to characterize the southern Appalachian region’s compositional and structural variability, we will present results from calculations of elastic tensors derived from electron backscatter diffraction (EBSD) data from a variety of metamorphic rocks with different mineral compositions. Here we will report analyses from 14 samples that are distributed into two transects across the Blue Ridge. Our goal is to address how deformational fabric controls the strength of mineral CPOs and thus seismic anisotropy

Detección de compuestos orgánicos mediante LIBS en rocas de interés en exploración planetaria. Aplicaciones en Astrobiología

La integración de instrumentos LIBS en misiones de exploración planetaria es una realidad desde hace ya unos años, siendo una tecnología conducente a la obtención de información multielemental en las distintas rocas y minerales existentes en la superficie de Marte. Su gran eficacia ha sido demostrada en muchos de los trabajos publicados hasta la fecha por los equipos de investigación participantes en la misión Mars Science Laboratory (MSL). Uno de los objetivos primordiales de dichas investigaciones radica en la detección de posibles bioindicadores [1], así como en la identificación y discriminación mediante LIBS de compuestos orgánicos, tarea que puede llegar a ser compleja ya que, entre otros aspectos, se trata de una técnica muy sensible a las condiciones ambientales (composición de la atmósfera y presión existente) [2] [3]. El presente trabajo ha buscado por un lado, evaluar el efecto de la atmósfera existente en el planeta rojo (rica en CO2 con 7mb de presión media) en la formación de plasmas inducidos por láser a partir de la ablación de muestras formadas por matrices inorgánicas dopadas con compuestos orgánicos seleccionados. Por otro lado, a partir de las diferentes huellas espectrales obtenidas en dichas condiciones y mediante la aplicación de técnicas quimiométricas adaptadas, se estudian las posibilidades de identificación de dichos referentes orgánicos.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech