Uptake and utilization of 'colloidal DOM' by heterotrophic flagellates in seawater

A variety of macromolecules labeled with FITC (fluorescein isothiocyanate), including carbohydrates (dextran) and proteins (ferritin, casein, albumin, concavalin A), spanning a range of molecular weights from 55 to 2000 kD, were actively ingested by marine heterotrophic flagellates at substrate concentrations of 1 to 10 mg/L. The uptake capacity for the macromolecules was positively correlated to clearance rate for bacterial-sized prey Ingested FITC appeared In the food vacuoles of the cells The biomass of a cultured bacterivorous flagellate species Increased in cultures amended with 10 mg of 2000 kD dextran/L to an extent that could not be explained by bacterivory However. 40 kD devtran did not stimulate flagellate growth On the basis of these results, we suggest that protists have the capacity to ingest colloids of widely different sizes, spanning the size range of bacteria and larger organisms to macromolecules possibly including the recently discovered abundant non-living submicrometer colloids in sea water

Absence of a priming effect on dissolved organic carbon degradation in lake water

The idea that small amounts of labile organic carbon might trigger the degradation of previously unreactive organic matter has attracted increasing scientific interest across multiple disciplines. Although this phenomenon, referred to as priming, has been widely reported in soils, evidence in freshwater systems is scarce and inconclusive. Here, we use a multifactorial microcosm experiment to test the conditions under which priming may be observed in freshwater ecosystems. We assessed the effect of pulse additions of three labile carbon sources (acetate, glucose, and cellobiose) on dissolved organic carbon (DOC) consumption using water from lakes with different trophic states (eutrophic to oligotrophic and clear to brownwater lakes). We further analyzed the effect of nutrient availability and the role of attachment of cells to surfaces. Despite the range of conditions tested, we found no clear evidence of a priming effect on DOC degradation, indicating that priming in freshwater systems may be of limited importance

Матеріали міжнародної науково-практичної конференції „Маркетинговий інструментарій управління попитом на товари та послуги“

Матеріали міжнародної науково-практичної конференції є результатом наукових досліджень авторів з проблем розроблення концептуальних засад маркетингового інструментарію управління попитом на товари і послуги України

Comprehensive analysis of chemical and biological problems associated with browning agents used in aquatic studies

Inland waters receive and process large amounts of colored organic matter from the terrestrial surroundings. These inputs dramatically affect the chemical, physical, and biological properties of water bodies, as well as their roles as global carbon sinks and sources. However, manipulative studies, especially at ecosystem scale, require large amounts of dissolved organic matter with optical and chemical properties resembling indigenous organic matter. Here, we compared the impacts of two leonardite products (HuminFeed and SuperHume) and a freshly derived reverse osmosis concentrate of organic matter in a set of comprehensive mesocosm- and laboratory-scale experiments and analyses. The chemical properties of the reverse osmosis concentrate and the leonardite products were very different, with leonardite products being low and the reverse osmosis concentrate being high in carboxylic functional groups. Light had a strong impact on the properties of leonardite products, including loss of color and increased particle formation. HuminFeed presented a substantial impact on microbial communities under light conditions, where bacterial production was stimulated and community composition modified, while in dark potential inhibition of bacterial processes was detected. While none of the browning agents inhibited the growth of the tested phytoplankton Gonyostomum semen, HuminFeed had detrimental effects on zooplankton abundance and Daphnia reproduction. We conclude that the effects of browning agents extracted from leonardite, particularly HuminFeed, are in sharp contrast to those originating from terrestrially derived dissolved organic matter. Hence, they should be used with great caution in experimental studies on the consequences of terrestrial carbon for aquatic systems

Global abundance and size distribution of streams and rivers

To better integrate lotic ecosystems into global cycles and budgets, we provide approximations of the size-distribution and areal extent of streams and rivers. One approach we used was to employ stream network theory combined with data on stream width. We also used detailed stream networks on 2 continents to estimate the fraction of continental area occupied by streams worldwide and corrected remote sensing stream inventories for unresolved small streams. Our estimates of global fluvial area are 485 000 to 662 000 km2 and are +30–300% of published appraisals. Moderately sized rivers (orders 5–9) seem to comprise the greatest global area, with less area covered by low and high order streams, while global stream length, and therefore the riparian interface, is dominated by 1st order streams. Rivers and streams are likely to cover 0.30–0.56% of the land surface and make contributions to global processes and greenhouse gas emissions that may be +20–200% greater than those implied by previous estimates