1,753 research outputs found

    Bone marrow-derived mesenchymal stem cells characterization and transplantation in an animal model of congenital hydrocephalus

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    Congenital hydrocephalus is a disorder presenting a degeneration of the periventricular cerebral parenchyma and the white matter, which causes significant mortality and life-long neurological complications. There are currently no effective therapies for congenital hydrocephalus. Bone marrow-derived mesenchymal stem cells (BM-MSC) are considered as a potential therapeutic tool in neurodegenerative diseases, due to their ability to migrate to degenerated tissues and the production of growth factors. In the present study, using an animal model of congenital hydrocephalus, the hyh mouse, it has been studied the capacity of the BM-MSC to reach the degenerated regions exhibiting glial reactions and their probable neuroprotector effects. The BM-MSC were isolated from two different sources: a) transgenic mice expressing the monomeric red fluorescent protein (mRFP1); b) wild type mice. In the second case, the BM-MSC were labelled in vitro using bromodeoxyuridine, a fluorescent cell tracker and the lipophilic DiR. Before application, the cells were analysed using flow cytometry and immunofluorescence. The BM-MSC were injected into the retro-orbital sinus or into the lateral ventricle of hyh mice. After 24/96 hours of administration, the BM-MSC were detected under light, confocal and electron microscopes. The injected BM-MSC reached the degenerated periventricular regions and the disrupted neurogenic niches. They were detected in the periventricular parenchyma, around periventricular blood vessels and in the ventral meninges. Most of the applied BM-MSC expressed the glial cell-derived neurotrophic factor (GDNF), in the same way as the periventricular reactive astrocytes, suggesting a possible neuroprotector effect.FIS (Instituto de Salud Carlos III)-FEDER a AJJ. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

    Characterization and administration of bone marrow-derived mesenchymal stem cells in an animal model of congenital hydrocephalus

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    Bone marrow-derived mesenchymal stem cells (BM-MSC) are considered as a potential therapeutic tool in neurodegenerative diseases, due to their ability to migrate to degenerated tissues and the production of growth factors. Congenital hydrocephalus is a disorder characterized by a degeneration of the periventricular cerebral parenchyma and the white matter. In the present study, using an animal model of congenital hydrocephalus, the hyh mouse, it has been studied the capacity of the BM-MSC to reach the degenerated regions exhibiting glial reactions and their probable neuroprotector effects. The BM-MSC were isolated from two sources: a) transgenic mice expressing the monomeric red fluorescent protein (mRFP1); b) wild type mice. In the second case, the BM-MSC were labelled in vitro using bromodeoxyuridine, a fluorescent cell tracker and the lipophilic DiR. Before application, the cells were analysed using flow cytometry and immunofluorescence. The BM-MSC were injected into the retro-orbital sinus or into the lateral ventricle of hyh mice. After 24/96 hours of administration, they were detected under light, confocal and electron microscopes. The injected BM-MSC reached the degenerated periventricular regions and the disrupted neurogenic niches. They were detected in the periventricular parenchyma, around periventricular blood vessels and in the ventral meninges. Most of the applied BM-MSC expressed the glial cell-derived neurotrophic factor (GDNF), in the same way as the periventricular reactive astrocytes, suggesting a possible neuroprotector effect.Universidad de Málaga, Campus de Excelencia Internacional Andalucía Tech. Instituto de Salud Carlos III, PI12/0631 con cofinanciación FEDER

    Efficacy comparative of a validated power play training protocol in water polo U12 players

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    El presente estudio pretende comprobar la eficacia de un protocolo para la enseñanza de las situaciones de juego en desigualdad numérica temporal simple en waterpolo con jugadores alevines. Se diseñaron y aplicaron 20 sesiones teóricas y prácticas sobre las acciones correctas a realizar. Se utilizó la metodología observacional mediante un diseño descriptivo y correlacional de carácter longitudinal. Se analizaron las diferencias entre un grupo experimental y un grupo control en 160 microsituaciones de juego en relación a la consecución de gol, fallo, interceptación y duración de la jugada. Hallando diferencias significativas entre los grupos (÷²(2)=16.99; p<.001; TE=.23), más goles y menos interceptaciones en el grupo experimental. Se concluye que el protocolo utilizado con el grupo experimental es válido, fiable y útil para la enseñanza de las jugadas en desigualdad numérica temporal simple con y sin posesión del balón en waterpoloThe present study aims to test the efficacy of a protocol for teaching situations of play in simple temporary numerical inequality in water polo U12 player. 20 theoretical and practical sessions were designed and applied on the correct actions to be taken. Observational methodology was used through a descriptive and correlational longitudinal design. The differences between an experimental group and a control group were analyzed in 160 playing microsituations in relation to the achievement of goal, failure, interception and duration of the play. There were significant differences between groups (÷²(2)=16.99, p<.001; TE=.23), finding more goals and fewer interceptions in the experimental group. It is concluded that the protocol used with the experimental group is valid, reliable and useful for the teaching of plays in simple temporary numerical inequality with and without ball possession in water pol

    Throwing velocity in water polo elite competition: Analysis of associated variables

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    Throwing velocity is a relevant variable in water polo performance. Few studies have investigated the throw’s speed during an official competition or real game situation in high level competition. All throws performed in 27th European Championship in 2006 (Belgrade, Serbia) and 12th World Championships in 2007 (Melbourne, Australia) were analyzed in the present study. The total sample was composed of 5,691 throws (2,474 in female category). The study was developed with an observational design. A digital video camera, a radar and Polo Análisis Directo v1.0 software were used to record data. The reliability between the observers was verified using the kappa agreement index, ensuring that in all cases this value was greater than .85. The average maximum speed of throws in female water polo was 13.88 m/s (±2.44 m/s) and 16.94 m/s (±3.38 m/s) in male. The variables distance and game situation showed a combined ability to predict 19% of speed differences in female water polo throws and 33% in male

    Effect of rules changes on water polo shooting performance according to the final classification: high, medium, and worst level

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    Introduction: The aim of this study compare the influence of rules changes on shots performance considering three different levels, in the final classification, between two male European championships with different rules. Material and Methods: All shots made by the first, second, sixth, seventh, eleventh and twelfth classified in 27th European Championship in 2006 (Belgrade, Serbia) and all shots made by the first, second, eighth, ninth, fifteenth and sixteenth classified in 34th European Championship in 2020 (Budapest, Hungary) were analysed. Results: The total sample was composed of 3,467 shots (1,813 in Belgrade Championship and 1,654 in Budapest). The study was developed with an observational design. Three observers with more than 300h of experience in observational studies of water polo consensually quantified all the actions. Discussion: The effectiveness of the shots, considering only those that have taken place in equality and counterattack, shows greater scoring efficiency by the HL teams against the WL; and specifically among the shots made with a balanced period score (+-1) (F = 3.637; p = .032; S.E. = .107) and unbalanced (+-2) (F = 3.835; p = .027; S.E. = .106) stand out. In inequality situations its noted the existence of lower efficiency for WL teams when shot from the center stands out against the HL. Conclusions: It is concluded that the regulatory changes have reduced the importance, in the performance of the teams, of effectiveness in situations of inequality, giving rise to a more dynamic game, balanced according to the skill of the players and not their body size, but above all less stati

    Origen, evolución e influencia de las reglas de waterpolo sobre la dinámica del juego

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    The aim of this study was to describe the origin and evolution of the different regulations in water polo that have existed throughout its almost one hundred and fifty years of history and their possible influence on the game dynamics. A content analysis of the texts published on paper by the Fédération Internationale de Natation was carried out, focusing on semantics, based on some key categories of this sport. The main conclusion is that many regulatory changes have been made over the years. However, there is no scientific evidence of their effects on the game dynamicsEl objetivo de este estudio fue describir el origen y evolución de sus diferentes reglamentos a lo largo de sus casi ciento cincuenta años de historia y su influencia en la dinámica del juego. Se llevó a cabo un análisis de contenido de los textos publicados en papel por la Federación Internacional de Natación Amateur, centrándose en la semántica, a partir de algunas categorías clave de este deporte. Se concluye que han existido numerosos cambios normativos a lo largo de los años. Sin embargo, sigue sin existir evidencia científica de sus efectos en la dinámica del juegoS

    Shooting performance in high level women's water polo according to the final ranking

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    Introduction: The aim of this study was to analyze the situational framework (numerical equality, counterattack and numerical inequality) associated with shooting performance in women’s Water polo considering three different levels in the final ranking (high -1st-4th-, medium -5th-8th- and worst -9th-12th-). Material and Methods: All shots (2698) made in 34th European Championship in 2020 were analyzed in the present study. The study was developed with an observational design. The reliability between three observers was verified using the Kappa concordance index. The shots were registered using Polo Direct Analysis v1.0 software. Results: There are differences between the high level teams (ranked at 1st-4th) and the rest of the teams, appreciating a greater scoring efficiency in equality lob shots (.5; 1.0; 1.2) and reverse shot [.7; .5; .2]) inequality short post (3.0; 1.8; 1.7) and counterattack in the situations shots from left side (1.6; .7; .5), shots from center with feint (2.9; 1.5; .7), drive (2.0; .8; .7) and rebound shot (.9; .7; .3). Discussion: The existence of differences between the top-ranked teams, those in the intermediate zones and the lowest-ranked teams are found. There is a greater scoring efficiency in the top-ranked teams. Conclusions: It is concluded that in order to achieve a higher performance in women's water polo, athletes must have the ability to withstand great efforts and perform fast swims to convert counterattack situations. Likewise, and in static situations, it is necessary to have fast ball circulation, lining up players with a great versatility of shooting in the different game situations, which therefore allows them to have more resources and take advantage of the opportunities generated by the opponentS

    Self-efficacy perception in elite water polo goalkeepers

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    The sports in which there is the figure of goalkeeper, becomes relevant in order to obtain high performance, the perception of success that this participant is formed and that is configured as an expectation. Therefore, this study aims to analyse the association between perception of self-efficacy of two elite water polo goalkeepers and their coach and the observable performance in an international tournament. In order to measure the perception of behavioural success and their coach, it was used the Scale of achievement self-efficacy for water polo goalkeepers (Argudo, De la Vega, Tejero, & Ruiz, 2013). To measure the performance, were analysed all throwing’s received by the two goalkeepers in the XV Water polo World Championship. The results indicate that there are differences between the perception of the player, the coach and the observed behaviour, appreciating that the goalkeeper’s perception of success is superior to what actually happens. While in the female case, player and coach perception is less than the observed behaviour

    Effect of training in SSG on the ability to repeat sprints in young football players

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    Introduction: Football is one of the most popular team sports worldwide. During a match, players perform varying acyclic activities at differing intensities in order to face the complex and dynamic competition requirements. For this reason, the aim of this study was demonstrate that training in small sided is an effective method for developing the ability to repeat sprints in young football players. Material and Method: Fifty-four male soccer players (U13, U15 and U17, n=18 in which group) at the youth level participated in this study. A program based on Small Sided Games (3vs3) during 6 weeks in a space of 30x25m was applied. A contrast of means, pre and post intervention, was carried out between control and the experimental group and segregating the sample by age group. Results: When analyzing the degree of relationship between RSA fatigue index and jump loss, no significant differences were found. We also studied the relationship between the jump power (CMJ) and the power of the best sprint performed at RSA. Discusion: These results are consistent with those obtained in previous studies (Clemente et al., 2014; Dellal et al., 2012; Owen et al., 2012) on elite players. Conclusion: It can be concluded that this program improves the capacity of recovery before high intensity efforts and allows, at the same time, to work on technical and tactical elements and to develop the physical profile of the players. This type of games provokes improvements in the vertical jump, generate more power in the (CMJ) and the capacity to repeat explosive efforts

    Análisis de los valores de eficacia de los porteros de waterpolo

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    El presente estudio pretende analizar los valores de eficacia de los porteros del Campeonato del Mundo de Waterpolo celebrado en Melbourne en 2007. Con la intención de determinar si existen diferencias entre la condición de ganador y perdedor, entre ganadores, entre perdedores y entre sexos, se filmaron y analizaron los 96 partidos celebrados, utilizando el Software Polo Análisis Directo v1.0.Se midieron diversos coeficientes de eficacia en situaciones de igualdad, desigualdad, transición y penaltis. Se procedió con comparación de medias U de Mann-Whitney y se encontraron diferencias entre los coeficientes de resolución e imprecisión en situación de igualdad en función del sexo (U = .02; p < .05, U = .03; p < .05) y resultado (U = .0; p < .05, U = .0; p < .05), aunque no se apreciaron diferencias entre ganadores ni entre perdedores.En conclusión, se considera que los coeficientes de im-precisión en transición y penalti ofrecen una idea de la situación deportiva a analizar y entrenar para aumentar el rendimiento deportivo, aunque aún es necesaria mayor investigaciónThe present study aims to analyze the efficacy values of goalkeepers in the Water polo World Championships held in Melbourne in 2007. In order to determine any differences between winner and loser, winners, losers and between genres, were filmed and analyzed, using the Software Pole Direct Analysis v1.0, all the 96 match celebrated.Various coefficients of effectiveness were measured in situations of equality, inequality, transition and penalties. A comparison of U Mann-Whitney was performed, and we found differences between the coefficients of resolution and imprecision in a situation of equality according to sex (U = .02; p < .05, U = .03; p < .05) and results (U = .0; p < .05, U = .0; p < .05), although there were no differences between winners, or among losers.In conclusion, it is considered that the coefficients of imprecision in transition and penalty give an idea of the sport situation to analyze and train to increase the sport performance, although still more research is necessary.Actividad Física y Deport
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