The Splicing Factor Proline-Glutamine Rich (SFPQ/PSF) Is Involved in Influenza Virus Transcription

The influenza A virus RNA polymerase is a heterotrimeric complex responsible for viral genome transcription and replication in the nucleus of infected cells. We recently carried out a proteomic analysis of purified polymerase expressed in human cells and identified a number of polymerase-associated cellular proteins. Here we characterise the role of one such host factors, SFPQ/PSF, during virus infection. Down-regulation of SFPQ/PSF by silencing with two independent siRNAs reduced the virus yield by 2–5 log in low-multiplicity infections, while the replication of unrelated viruses as VSV or Adenovirus was almost unaffected. As the SFPQ/PSF protein is frequently associated to NonO/p54, we tested the potential implication of the latter in influenza virus replication. However, down-regulation of NonO/p54 by silencing with two independent siRNAs did not affect virus yields. Down-regulation of SFPQ/PSF by siRNA silencing led to a reduction and delay of influenza virus gene expression. Immunofluorescence analyses showed a good correlation between SFPQ/PSF and NP levels in infected cells. Analysis of virus RNA accumulation in silenced cells showed that production of mRNA, cRNA and vRNA is reduced by more than 5-fold but splicing is not affected. Likewise, the accumulation of viral mRNA in cicloheximide-treated cells was reduced by 3-fold. In contrast, down-regulation of SFPQ/PSF in a recombinant virus replicon system indicated that, while the accumulation of viral mRNA is reduced by 5-fold, vRNA levels are slightly increased. In vitro transcription of recombinant RNPs generated in SFPQ/PSF-silenced cells indicated a 4–5-fold reduction in polyadenylation but no alteration in cap snatching. These results indicate that SFPQ/PSF is a host factor essential for influenza virus transcription that increases the efficiency of viral mRNA polyadenylation and open the possibility to develop new antivirals targeting the accumulation of primary transcripts, a very early step during infection

Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker

Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one of the most important threats of Pinus spp. globally. This pathogen is known in many pine-growing regions, including natural and planted forests, and can affect all life stages of trees, from emerging seedlings to mature trees. Despite the importance of PPC, the global distribution of F. circinatum is poorly documented, and this problem is also true of the hosts within countries that are affected. The aim of this study was to review the global distribution of F. circinatum, with a particular focus on Europe. We considered (1) the current and historical pathogen records, both positive and negative, based on confirmed reports from Europe and globally; (2) the genetic diversity and population structure of the pathogen; (3) the current distribution of PPC in Europe, comparing published models of predicted disease distribution; and (4) host susceptibility by reviewing literature and generating a comprehensive list of known hosts for the fungus. These data were collated from 41 countries and used to compile a specially constructed geo-database. A review of 6297 observation records showed that F. circinatum and the symptoms it causes on conifers occurred in 14 countries, including four in Europe, and is absent in 28 countries. Field observations and experimental data from 138 host species revealed 106 susceptible host species including 85 Pinus species, 6 non-pine tree species and 15 grass and herb species. Our data confirm that susceptibility to F. circinatum varies between different host species, tree ages and environmental characteristics. Knowledge on the geographic distribution, host range and the relative susceptibility of different hosts is essential for disease management, mitigation and containment strategies. The findings reported in this review will support countries that are currently free of F. circinatum in implementing effective procedures and restrictions and prevent further spread of the pathogen

Daily reference evapotranspiration modeling by using genetic programming approach in the Basque Country (Northern Spain)

Evapotranspiration, as a major component of the hydrological cycle, is of importance for water resources management and development, as well as for estimating the water budget of irrigation schemes. This study presents a Gene Expression Programming (GEP) approach, for estimating daily reference evapotranspiration (ET0) in four weather stations in Basque Country (Northern Spain), for a 5-year period (1999-2003). The data set comprising air temperature, relative humidity, wind speed and solar radiation was employed for modeling ET0 using FAO-56 Penman Monteith equation as the reference. The GEP results were compared with the Adaptive Neuro-Fuzzy Inference System (ANFIS), Priestley-Taylor and Hargreaves-Samani models. Based on the comparisons, the GEP was found to perform better than the ANFIS, Priestley-Taylor and Hargreaves-Samani models. The ANFIS model is ranked as the second best model

Fibrosing Mediastinitis Caused by Histoplasmosis in an Adolescent

Fibrosing mediastinitis (FM) is a rare, potentially progressive disease resulting from an idiosyncratic immune response to a variety of stimuli that lead to fibrous infiltration of the mediastinum and possible narrowing of the bronchovascular structures. We report an unusual case of FM in a pediatric patient presenting as myopericarditis and progressing to pericardial thickening and encasement of the mediastinal vascular structures needing surgical intervention. Imaging, including transthoracic echocardiography, cardiac computed tomography, and cardiac magnetic resonance played a crucial role in the diagnosis, assessment, and follow-up. Contrast-enhanced computed tomography can be especially helpful to demonstrate potential findings associated with FM

A new approach for developing comprehensive agricultural drought index using satellite-derived biophysical parameters and factor analysis method

The accurate assessment of drought and its monitoring is highly depending on the selection of appropriate indices. Despite the availability of countless drought indices, due to variability in environmental properties, a single universally drought index has not been presented yet. In this study, a new approach for developing comprehensive agricultural drought index from satellite-derived biophysical parameters is presented. Therefore, the potential of satellite-derived biophysical parameters for improved understanding of the water status of pistachio (Pistachio vera L.) crop grown in a semiarid area is evaluated. Exploratory factor analysis with principal component extraction method is performed to select the most in?uential parameters from seven biophysical parameters including surface temperature (Ts), surface albedo (a), leaf area index (LAI), soil heat ?ux (Go), soil-adjusted vegetation index (SAVI), normalized difference vegetation index (NDVI), and net radiation (Rn). Ts and Gowere found as the most effective parameters by this method. However, Ts, LAI, a, and SAVI that accounts for 99.6 % of the total variance of seven inputs were selected to model a new biophysical water stress index (BPWSI). The values of BPWSI were stretched independently and compared with the range of actual evapotranspiration estimated through well-known METRIC (mapping evapotranspiration at high resolution with internal calibration) energy balance model. The results showed that BPWSI can be ef?ciently used for the prediction of the pistachio water status (RMSE of 0.52, 0.31, and 0.48 mm/day on three image dates of April 28, July 17, and August 2, 2010). The study con?rmed that crop water status is accounted by several satellite-based biophysical parameters rather than single parameter