Report on estimating the size of dolphin schools, based on data obtained during a charter cruise of the M/V Gina Anne, October 11 -November 25, 1979

Estimates of dolphin school sizes made by observers and crew members aboard tuna seiners or by observers on ship or aerial surveys are important components of population estimates of dolphins which are involved in the yellowfin tuna fishery in the eastern Pacific. Differences in past estimates made from tuna seiners and research ships and aircraft have been noted by Brazier (1978). To compare various methods of estimating dolphin school sizes a research cruise was undertaken with the following major objectives: 1) compare estimates made by observers aboard a tuna seiner and in the ship's helicopter, from aerial photographs, and from counts made at the backdown channel, 2) compare estimates of observers who are told the count of the school size after making their estimate to the observer who is not aware of the count to determine if observers can learn to estimate more accurately, and 3) obtain movie and still photographs of dolphin schools of known size at various stages of chase, capture and release to be used for observer training. The secondary objectives of the cruise were to: 1) obtain life history specimens and data from any dolphins that were killed incidental to purse seining. These specimens and data were to be analyzed by the U.S. National Marine Fisheries Service ( NMFS ) , 2) record evasion tactics of dolphin schools by observing them from the helicopter while the seiner approached the school, 3) examine alternative methods for estimating the distance and bearing of schools where they were first sighted, 4) collect the Commission's standard cetacean sighting, set log and daily activity data and expendable bathythermograph data. (PDF contains 31 pages.

Preference ranking of selected carbohydrate energy staples by very active manual workers in Nairobi, Kenya

In Kenya, “Ugali”, made from milled whole sifted dry maize (corn) flour, is the popular staple food consumed by nearly 80% of the population. The  overreliance on “Ugali” has posed a major problem socially, economically and politically. Ugali is generally perceived to have higher energy and  satiety compared to other carbohydrate staple foods. A descriptive cross-sectional study was conducted using 322 purposefully selected  respondents. The objective was to determine the preference ranking of selected commonly consumed carbohydrate energy staple foods by Very  Active Manual Workers (VAMW). The VAMW require high energy in order to sustain their very active lifestyle. Three group categories were targeted:  VAMW, moderately active Civil servants (CS) and University students (US). Structured questionnaires were administered to respondents to indicate  their top ranking and preferred staple foods among six commonly consumed carbohydrate energy foods - Ugali, rice, chapati, Irish potatoes,  bananas (Matoke), and “others” category (foods such as Githeri (mixed maize and beans), cassava, spaghetti, noodles, Sweet potatoes, and bread  among many alternatives). The foods were ranked according to the most preferred to the least preferred on a scale of 1 to 6. Results indicated a  higher preference for Ugali by all respondent groups. The mean rank for Ugali by VAMW was 1.134±0.492, followed by chapati 2.588±0.976, third  was rice 3.27±1.242, fourth was Irish Potatoes 4.299±0.970, fifth bananas (matoke) 4.588±1.068, and sixth was “others” 5.165±1.296. US preference  ranking for Ugali was 1.628±1.067, rice 2.5±1.085, chapati 2.564±1.083, Irish potatoes 4.053±0.943, bananas 4.394±0.918, and others 5.872±0.421;  CS ranking for Ugali was 1.471±0.930, rice 2.608±1.101, chapati 3.09±1.264, bananas 4.029±1.173, Irish potatoes 4.51±0.972, and others  5.284±1.396. Combined groups mean was 1.410±0.064 and food preference ranking was very significantly different, P < 0.0001. Pairwise comparison  of mean ranks indicated a significant difference between rank means of all groups, but there was no significant difference between the  ranking for rice versus chapati, and Irish potatoes versus bananas. The VAMW had a higher preference for Ugali compared to the moderately  active groups.&nbsp

The rapid evolution of the born-again giant Sakurai’s object

The extraordinarily rapid evolution of the born-again giant Sakurai’s object following discovery in 1996 has been investigated. The evolution can be traced both in a continued cooling of the stellar surface and dramatic changes in chemical composition on a timescale of a mere few months. The abundance alterations are the results of the mixing and nuclear reactions which have ensued due to the final He-shell flash which occurred during the descent along the white dwarf cooling track. The observed changes in the H and Li abundances can be explained by ingestion and burning of the H-rich envelope and Li-production through the Cameron-Fowler mechanism. The rapidly increasing abundances of the light s-elements (including Sc) are consistent with current s-processing by neutrons released from the concomitantly produced 13C. However, the possibility that the s-elements have previously been synthesized during the AGB-phase and only mixed to the surface in connection with the final He-shell flash in the pre-white dwarf cannot be convincingly ruled out either. Since Sakurai’s object shows substantial abundance similarities with the R CrB stars and has recently undergone R CrB-like visual fading events, the “birth” of an R CrB star may have been witnessed for the first time ever. Sakurai’s object thus lends strong support for the suggestion that at least some of the R CrB stars have been formed through a final He-shell flash in a post-AGB star

Current induced switching of magnetic domains to a perpendicular configuration

In a ferromagnet--normal-metal--ferromagnet trilayer, a current flowing perpendicularly to the layers creates a torque on the magnetic moments of the ferromagnets. When one of the contacts is superconducting, the torque not only favors parallel or antiparallel alignment of the magnetic moments, as is the case for two normal contacts, but can also favor a configuration where the two moments are perpendicular. In addition, whereas the conductance for parallel and antiparallel magnetic moments is the same, signalling the absence of giant magnetoresistance in the usual sense, the conductance is greater in the perpendicular configuration. Thus, a negative magnetoconductance is predicted, in contrast with the usual giant magnetoresistance.Comment: 4 pages, 3 figures, major rewriting of the technical par

Distributed expertise: Qualitative study of a British network of multidisciplinary teams supporting parents of children with chronic kidney disease

© 2014 The Authors. Background: Long-term childhood conditions are often managed by hospital-based multidisciplinary teams (MDTs) of professionals with discipline specific expertise of a condition, in partnership with parents. However, little evidence exists on professional-parent interactions in this context. An exploration of professionals' accounts of the way they individually and collectively teach parents to manage their child's clinical care at home is, therefore, important for meeting parents' needs, informing policy and educating novice professionals. Using chronic kidney disease as an exemplar this paper reports on one aspect of a study of interactions between professionals and parents in a network of 12 children's kidney units in Britain. Methods: We conducted semi-structured, qualitative interviews with a convenience sample of 112 professionals (clinical-psychologists, dietitians, doctors, nurses, pharmacists, play-workers, therapists and social workers), exploring accounts of their parent-educative activity. We analysed data using framework and the concept of distributed expertise. Results: Four themes emerged that related to the way expertise was distributed within and across teams: (i) recognizing each other's' expertise, (ii) sharing expertise within the MDT, (iii) language interpretation, and (iv) acting as brokers. Two different professional identifications were also seen to co-exist within MDTs, with participants using the term 'we' both as the intra-professional 'we' (relating to the professional identity) when describing expertise within a disciplinary group (for example: 'As dietitians we aim to give tailored advice to optimize children's growth'), and the inter-professional 'we' (a 'team-identification'), when discussing expertise within the team (for example: 'We work as a team and make sure we're all happy with every aspect of their training before they go home'). Conclusions: This study highlights the dual identifications implicit in 'being professional' in this context (to the team and to one's profession) as well as the unique role that each member of a team contributes to children's care. Our methodology and results have the potential to be transferred to teams managing other conditions

Impact of zumor mutation burden on nivolumab efficacy in second-line urothelial carcinoma patients: Exploratory analysis of the phase ii checkmate 275 study

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False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011

The gonococcal porA pseudogene is a popular target for in-house Neisseria gonorrhoeae PCR methods. With this study we present two novel findings: the first case of an N. gonorrhoeae porA pseudogene PCR false-negative result caused by sequence variation, and in the same organism, the first description of a clinical N. gonorrhoeae strain harbouring an N. meningitidis porA sequence

Chemostratigraphy of Neoproterozoic carbonates: implications for 'blind dating'

The delta C-13(carb) and Sr-87/Sr-86 secular variations in Neoproteozoic seawater have been used for the purpose of 'isotope stratigraphy' but there are a number of problems that can preclude its routine use. In particular, it cannot be used with confidence for 'blind dating'. The compilation of isotopic data on carbonate rocks reveals a high level of inconsistency between various carbon isotope age curves constructed for Neoproteozoic seawater, caused by a relatively high frequency of both global and local delta C-13(carb) fluctuations combined with few reliable age determinations. Further complication is caused by the unresolved problem as to whether two or four glaciations, and associated negative delta C-13(carb) excursions, can be reliably documented. Carbon isotope stratigraphy cannot be used alone for geological correlation and 'blind dating'. Strontium isotope stratigraphy is a more reliable and precise tool for stratigraphic correlations and indirect age determinations. Combining strontium and carbon isotope stratigraphy, several discrete ages within the 590-544 Myr interval, and two age-groups at 660-610 and 740-690 Myr can be resolved

The function of CozE proteins is linked to lipoteichoic acid biosynthesis in Staphylococcus aureus.

Coordinated membrane and cell wall synthesis is vital for maintaining cell integrity and facilitating cell division in bacteria. However, the molecular mechanisms that underpin such coordination are poorly understood. Here we uncover the pivotal roles of the staphylococcal proteins CozEa and CozEb, members of a conserved family of membrane proteins previously implicated in bacterial cell division, in the biosynthesis of lipoteichoic acids (LTA) and maintenance of membrane homeostasis in Staphylococcus aureus. We establish that there is a synthetic lethal relationship between CozE and UgtP, the enzyme synthesizing the LTA glycolipid anchor Glc <sub>2</sub> DAG. By contrast, in cells lacking LtaA, the flippase of Glc <sub>2</sub> DAG, the essentiality of CozE proteins was alleviated, suggesting that the function of CozE proteins is linked to the synthesis and flipping of the glycolipid anchor. CozE proteins were indeed found to modulate the flipping activity of LtaA in vitro. Furthermore, CozEb was shown to control LTA polymer length and stability. Together, these findings establish CozE proteins as novel players in membrane homeostasis and LTA biosynthesis in S. aureus.IMPORTANCELipoteichoic acids are major constituents of the cell wall of Gram-positive bacteria. These anionic polymers are important virulence factors and modulators of antibiotic susceptibility in the important pathogen Staphylococcus aureus. They are also critical for maintaining cell integrity and facilitating proper cell division. In this work, we discover that a family of membrane proteins named CozE is involved in the biosynthesis of lipoteichoic acids (LTAs) in S. aureus. CozE proteins have previously been shown to affect bacterial cell division, but we here show that these proteins affect LTA length and stability, as well as the flipping of glycolipids between membrane leaflets. This new mechanism of LTA control may thus have implications for the virulence and antibiotic susceptibility of S. aureus

National predictors of influenza vaccine uptake in pregnancy: the FluMum prospective cohort study, Australia, 2012–2015

Objective: Ascertain predictors of inactivated influenza vaccine (IIV) uptake in pregnancy in mother–infant pairs from six Australian sites over four consecutive influenza seasons (2012–2015). Methods: Prospective observational cohort study calculating proportions of unvaccinated and vaccinated pregnancies. Multivariable logistic regression calculating adjusted odds ratios (aOR) and 95% confidence intervals (95%CI) to determine demographic, pregnancy and birth characteristics as predictors of IIV uptake in pregnancy. Results: Uptake of IIV was 36% (n=3,651/9,878) with only 3–4% during the first trimester. Validation of IIV receipt was obtained for 77% of vaccinated participants. Predictors of IIV uptake in pregnancy were: healthcare provider recommendation to have IIV during pregnancy (aOR 7.04 [95%CI 5.83-8.50]): GP (aOR 4.12 [95%CI 3.43-4.98]), obstetrician (aOR 4.41 [95%CI 3.45-5.64]), midwife (aOR 1.88 [95%CI 1.51-2.36]); previous IIV within 12 months of their current pregnancy (aOR 2.87 [95%CI 2.36-3.50]); and pertussis vaccination during the current pregnancy (aOR 4.88 [95%CI 4.08-5.83]). Conclusions and implications for public health: Healthcare provider discussions with pregnant women about the risks associated with influenza infection during pregnancy and early infancy and evidence about the safety and effectiveness of IIV are required. Recommending and offering IIV in pregnancy needs to be included in these discussions to improve uptake.Lisa McHugh, Kerry-Ann F. O’Grady, Terry Nolan, Peter C. Richmond, Nicholas Wood, Helen S. Marshall, Stephen B. Lambert, Mark D. Chatfield, Kirsten P. Perrett, Paula Binks, Michael J. Binks, Ross M. Andrew