Temas de vida de estudantes universitários : um estudo exploratório da entrevista de construção de carreira

Tese de mestrado, Psicologia (Secção de Psicologia da Educação e da Orientação), Universidade de Lisboa, Faculdade de Psicologia, 2015A Entrevista de Construção de Carreira (ECC) tem sido uma das técnicas mais utilizadas em aconselhamento vocacional nos últimos anos. Porém, são ainda escassas as investigações que exploram a relação dos temas de vida com diferentes percursos de carreira. O presente trabalho procura analisar os temas de vida e projetos vocacionais de estudantes de diferentes cursos universitários, a partir do questionário desenvolvido e adaptado da ECC. Esta investigação envolveu 148 participantes de faculdades Portuguesas nas duas etapas do estudo. Numa primeira etapa, realizaram-se entrevistas a seis participantes com idades compreendidas entre os 19 e os 22 anos, dos cursos de Psicologia, Gestão, Economia, Engenharia Civil e Ciências da Arte e do Património. O objetivo desta etapa foi analisar as potencialidades das diferentes questões da ECC e selecioná-las para a elaboração do Questionário de Construção de Projetos de Carreira (QCPC). Na segunda etapa do estudo, responderam ao QCPC 142 estudantes universitários, entre os 18 e os 50 anos, de três cursos superiores (Psicologia, Economia e Artes). Os instrumentos utilizados foram a Entrevista de Estilo de Vida para Estudantes Universitários e o QCPC, que envolvem as questões da ECC, o questionário de satisfação com o curso e a descrição dos projetos vocacionais. Utilizaram-se as dimensões RIASEC e as virtudes e forças de caráter na categorização das respostas. Apesar de na análise por grupos os participantes apresentarem temas de vida heterogéneos, existem algumas dimensões em comum: partilham como objetivo a Realização nos projetos de carreira e as forças de caráter bondade, amor e bravura. Os grupos Psicologia, Economia e Artes, respetivamente, caracterizam-se pelos tipos de personalidade Social, Empreendedor e Realista, e Artístico. Os resultados da presente investigação apoiam a utilidade da ECC. No final, discutem-se as implicações e aplicações práticas do estudo.In the last years, Career Construction Interview (CCI) has been one of the most used techniques in vocational counseling. However, there are few investigations exploring the relation between life themes and different career paths. This research aims to analyze life themes and vocational projects of students from different college majors with developed questionnaire adapted from CCI. This investigation involved 148 participants from Portuguese colleges in two phases of the study. On the first phase, interviews were conducted to six participants between 19 and 22 years old, from Psychology, Management, Economics, Civil Engineering and Art and Heritage Sciences. The purpose of this phase was to analyze the potentialities of the CCI questions and select them to buid the Career Projects Construction Questionnaire (CPCQ). In the second phase of the study, 142 college students between 18 and 50 years old from three college majors (Psychology, Economics and Arts) answered to the CPCQ. The applied instruments were Life Style Interview for College Students and CPCQ that includes questions from CCI, major satisfaction questionnaire and a description of vocational projects. RIASEC dimensions and Character Strengths and Virtues were used to code the answers. Despite the analysis by groups showed heterogeneous life themes, there are some dimensions in common: participants share the goal of Achievement in career projects and as Character Strengths, they value kindness, love and bravery. Psychology, Economics and Arts groups are respectively characterized by Social, Enterprising and Realistic, and Artistic personality types. The findings of this research support the usefulness of CCI. At the end, implications and practical applications of the study are discussed

Os desenhos que nascem das palavras

Relatório da Prática de Ensino Supervisionada, Mestrado em Ensino de Artes Visuais, Universidade de Lisboa, Instituto de Educação, 2019O presente relatório, realizado no âmbito da Prática de Ensino Supervisionada, apresenta e descreve o processo de implementação de uma unidade de trabalho, intitulada Os Desenhos que Nascem das Palavras, desenvolvida para uma turma do 11º ano do Ensino Secundário, na disciplina de Desenho A, da Escola Secundária Rainha Dona Leonor, em Alvalade. A atividade decorreu durante o 2º período do ano letivo 2018/2019, e, analisado o programa de Desenho A, foi desenvolvido um trabalho com a duração de 6 aulas, cumprindo 13 horas e 30 minutos totais de lecionação. Partindo do objetivo geral, este projeto de investigação tem como objetivo principal responder a uma das necessidades básicas que os alunos demonstram – Desenvolvimento pessoal e a autonomia – aspeto referido nas Aprendizagens Essenciais em Articulação com o Perfil do Aluno (2018). Outro objetivo, secundário, mas crucial, foi a motivação dos alunos para esta disciplina. Para suportar o relatório escrito, é abordado: a educação para a cidadania e a tecnologia; algumas teorias de aprendizagem; uma breve história sobre a ilustração; a relação entre o texto e a imagem e a importância do desenho no ensino. A unidade de didática teve como ponto de partida a experimentação em sala de aula, e alguns exercícios relacionados com a atenção/observação; a ida à Fundação Calouste Gulbenkian; o uso do smartphone, e dos livros durante a pesquisa; o trabalho de ilustração com aguarela e um material que nunca tivessem utilizado; e finalmente o feedback construtivo para a melhoria dos colegas. No final, discutem-se as implicações e aplicações práticas do estudo.This report was carried out under the scope of the Supervised Practicum in Teaching, presents and describes the process of implementation of a work unit, entitled Drawings coming out from Words, developed for a class of the 11th grade of Secondary Education, in the subject of Drawing A, from Rainha Dona Leonor Secondary School, in Alvalade. The activity took place during the 2nd period of the academic year 2018/2019, and after analysing the Drawing A program, it was developed a work with the duration of 6 classes, completing 13 hours and 30 minutes total of teaching. Starting from the general objective, this research project has as its main objective to answer one of the basic needs that students demonstrate – Personal development and autonomy – aspect mentioned in the Essential Learning in Articulation with the Student Profile (2018). Another secondary but crucial goal was student motivation for this subject. To support the written report, the following are addressed: education in citizenship and technology; some learning theories; a brief history of illustration is covered; the relationship between text and image; the importance of drawing in teaching and the process and development in learning. The didactic unit had as its starting point the experimentation in the classroom, and some exercises related to attention / observation; the trip to the Calouste Gulbenkian Foundation; the use of the smartphone and books during the research; illustration work with watercolor and a material the students had never used and finally constructive feedback for improvement of colleagues. Finally, the implications and practical applications of the study are discussed

Probing the Effects of the Well-mixed Assumption on Viral Infection Dynamics

Viral kinetics have been extensively studied in the past through the use of spatially well-mixed ordinary differential equations describing the time evolution of the diseased state. However, emerging spatial structures such as localized populations of dead cells might adversely affect the spread of infection, similar to the manner in which a counter-fire can stop a forest fire from spreading. In a previous publication (Beauchemin et al., 2005), a simple 2-D cellular automaton model was introduced and shown to be accurate enough to model an uncomplicated infection with influenza A. Here, this model is used to investigate the effects of relaxing the well-mixed assumption. Particularly, the effects of the initial distribution of infected cells, the regeneration rule for dead epithelial cells, and the proliferation rule for immune cells are explored and shown to have an important impact on the development and outcome of the viral infection in our model.Comment: LaTeX, 12 pages, 22 EPS figures, uses document class REVTeX 4, and packages float, graphics, amsmath, and SIunit

Processo para ativação de CO2 na etapa de regeneração de catalisadores de FCC desativados

DepositadaA presente invenção se refere a um processo que emprega um aditivo mais eficiente do que os encontrados no estado da técnica, para ativação de CO2 na etapa de regeneração de catalisadores de FFC gastos, sendo dito aditivo obtido a partir de um catalisador, que compreende um suporte de alumina, caulim, sílica ligante e aluminossilicato, por meio de modificação com metais de modificação com metais dos grupos I e II da tabela periódica dos elementos químicos como Li, Na, K, Cs, Mg e Ca

Green genes: bioinformatics and systems-biology innovations drive algal biotechnology.

Many species of microalgae produce hydrocarbons, polysaccharides, and other valuable products in significant amounts. However, large-scale production of algal products is not yet competitive against non-renewable alternatives from fossil fuel. Metabolic engineering approaches will help to improve productivity, but the exact metabolic pathways and the identities of the majority of the genes involved remain unknown. Recent advances in bioinformatics and systems-biology modeling coupled with increasing numbers of algal genome-sequencing projects are providing the means to address this. A multidisciplinary integration of methods will provide synergy for a systems-level understanding of microalgae, and thereby accelerate the improvement of industrially valuable strains. In this review we highlight recent advances and challenges to microalgal research and discuss future potential.We acknowledge support from the EU FP7 project SPLASH (Sustainable PoLymers from Algae Sugars and Hydrocarbons), grant agreement number 311956.This is the accepted manuscript. The final version is available from Cell/Elsevier at http://www.sciencedirect.com/science/article/pii/S016777991400196

The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: Highlights from a multi-level omics approach

<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas putida </it>KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by <it>P. putida </it>strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.</p> <p>Results</p> <p>We found that 26, 62, and 81% of the cell dry weight consist of PHA under conditions of carbon, dual, and nitrogen limitation, respectively. Under nitrogen limitation a specific PHA production rate of 0.43 (g·(g·h)^-1) was obtained. The residual biomass was not constant for dual- and strict nitrogen-limiting growth, showing a different feature in comparison to other <it>P. putida </it>strains. Dual limitation resulted in patterns of gene expression, protein level, and metabolite concentrations that substantially differ from those observed under exclusive carbon or nitrogen limitation. The most pronounced differences were found in the energy metabolism, fatty acid metabolism, as well as stress proteins and enzymes belonging to the transport system.</p> <p>Conclusion</p> <p>This is the first study where the interrelationship between nutrient limitations and PHA synthesis has been investigated under well-controlled conditions using a system level approach. The knowledge generated will be of great assistance for the development of bioprocesses and further metabolic engineering work in this versatile organism to both enhance and diversify the industrial production of PHAs.</p

Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction

<p>Abstract</p> <p>Background</p> <p><it>Burkholderia cenocepacia </it>is a threatening nosocomial epidemic pathogen in patients with cystic fibrosis (CF) or a compromised immune system. Its high level of antibiotic resistance is an increasing concern in treatments against its infection. Strain <it>B. cenocepacia </it>J2315 is the most infectious isolate from CF patients. There is a strong demand to reconstruct a genome-scale metabolic network of <it>B. cenocepacia </it>J2315 to systematically analyze its metabolic capabilities and its virulence traits, and to search for potential clinical therapy targets.</p> <p>Results</p> <p>We reconstructed the genome-scale metabolic network of <it>B. cenocepacia </it>J2315. An iterative reconstruction process led to the establishment of a robust model, <it>i</it>KF1028, which accounts for 1,028 genes, 859 internal reactions, and 834 metabolites. The model <it>i</it>KF1028 captures important metabolic capabilities of <it>B. cenocepacia </it>J2315 with a particular focus on the biosyntheses of key metabolic virulence factors to assist in understanding the mechanism of disease infection and identifying potential drug targets. The model was tested through BIOLOG assays. Based on the model, the genome annotation of <it>B. cenocepacia </it>J2315 was refined and 24 genes were properly re-annotated. Gene and enzyme essentiality were analyzed to provide further insights into the genome function and architecture. A total of 45 essential enzymes were identified as potential therapeutic targets.</p> <p>Conclusions</p> <p>As the first genome-scale metabolic network of <it>B. cenocepacia </it>J2315, <it>i</it>KF1028 allows a systematic study of the metabolic properties of <it>B. cenocepacia </it>and its key metabolic virulence factors affecting the CF community. The model can be used as a discovery tool to design novel drugs against diseases caused by this notorious pathogen.</p

Genetic mechanisms underlying spermatic and testicular traits within and among cattle breeds: systematic review and prioritization of GWAS results1

[EN] Reduced bull fertility imposes economic losses in bovine herds. Specifically, testicular and spermatic traits are important indicators of reproductive efficiency. Several genome-wide association studies (GWAS) have identified genomic regions associated with these fertility traits. The aims of this study were as follows: 1) to perform a systematic review of GWAS results for spermatic and testicular traits in cattle and 2) to identify key functional candidate genes for these traits. The identification of functional candidate genes was performed using a systems biology approach, where genes shared between traits and studies were evaluated by a guilt by association gene prioritization (GUILDify and ToppGene software) in order to identify the best functional candidates. These candidate genes were integrated and analyzed breeds. Results showed that GWAS for testicular-related traits have been developed for beef breeds only, whereas the majority of GWAS for spermatic-related traits were conducted using dairy breeds. comparing traits measured within the same study, the highest number of genes shared between different traits was observed, indicating a high impact of the population genetic structure and environmental effects. Several chromosomal regions were enriched for functional candidate genes associated with fertility traits. Moreover, multiple functional candidate genes were enriched for markers in a species-specific basis, taurine (Bos taurus) or indicine (Bos indicus). For the different candidate regions identified in the GWAS in the literature, functional candidate genes were detected as follows: B. Taurus chromosome X (BTX) (TEX11, IRAK, CDK16, ATP7A, ATRX, HDAC6, FMR1, L1CAM, MECP2, etc.), BTA17 (TRPV4 and DYNLL1), and BTA14 (MOS, FABP5, ZFPM2). These genes are responsible for regulating metabolic pathways or biological processes associated with fertility, such as progression of spermatogenesis, control of ciliary activity, development of Sertoli cells, DNA integrity in spermatozoa, and homeostasis of testicular cells. This study represents the first systematic review on male fertility traits in cattle using a system biology approach to identify key candidate genes for these traits.S