A Review of Vrushya Dravyas of Guduchyadi Varga of Bhavaprakasha Nighantu

Bhavaprakasha Nighantu, an important lexicon of Ayurveda consists of 23 Vargas where Guduchyadi Varga is one among them which consists of 190 drugs. Among them 28 drugs are attributed to Vrushya property. As Infertility has emerged as one of the most common health issues that many young couples are facing, there is a great demand for the treatment of infertility. While, the herbal therapies are better positioned to offer more holistic approaches to improve reproductive health. So here an attempt is made to screen the Vrushya Dravyas mentioned in Guduchyadi Varga of  Bhavaprakasha Nighantu with their Rasa, Guna, Veerya, Vipaka

In Vitro Safety Assessment of the Effect of Five Medicinal Plants on Human Peripheral Lymphocytes

Purpose: To evaluate, using ethnomedical data approach, five Indian plants used in traditional medicine for cancer and other diseases for their safety and cytotoxic activity on human lymphocytes.Methods: The antiproliferative effect of the 50, 100 and 200 ìg/ml aqueous extracts of five plants (leaves of Phyllanthus niruri, Coleus aromaticus, Azadirachta indica and Camellia sinensis, and driedfruit rind of Garcinia indica) were evaluated in vitro using trypan blue dye exclusion and clonogenic assay methods on two cell lines - Balb/c 3T3 mouse fibroblasts and human peripheral lymphocytes.Results: Among the five plants used traditionally to treat cancer and other infections, two of them (A. indica and C. aromaticus) exhibited cytotoxic effects on lymphocytes. Two other plants (G. indica and P.niruri) exhibited pronounced cytotoxic effects. Another plant (Camellia sinensis) exhibitedcytostimulatory effect (> 50 % cell proliferation). For the plants that are traditionally used in anticancer therapy, there was a correlation between the reported use of these plants and their cytotoxic activity onlymphocytes.Conclusion: The plant extracts of the leaves of P. niruri, C, aromaticus and A. indica, and the dried fruit rind of G. indica are cytotoxic to lymphocytes and this lends some credence to their traditional use for cancer treatment. However, green tea (C. sinensis) is cytostimulatory and safe for consumption

Nitrated fatty acids reverse cigarette smoke-induced alveolar macrophage activation and inhibit protease activity via electrophilic S-alkylation

Nitrated fatty acids (NFAs), endogenous products of nonenzymatic reactions of NO-derived reactive nitrogen species with unsaturated fatty acids, exhibit substantial anti-inflammatory activities. They are both reversible electrophiles and peroxisome proliferator-activated receptor γ (PPARγ) agonists, but the physiological implications of their electrophilic activity are poorly understood. We tested their effects on inflammatory and emphysema-related biomarkers in alveolar macrophages (AMs) of smoke-exposed mice. NFA (10-nitro-oleic acid or 12-nitrolinoleic acid) treatment downregulated expression and activity of the inflammatory transcription factor NF-κB while upregulating those of PPARγ. It also downregulated production of inflammatory cytokines and chemokines and of the protease cathepsin S (Cat S), a key mediator of emphysematous septal destruction. Cat S downregulation was accompanied by decreased AM elastolytic activity, a major mechanism of septal destruction. NFAs downregulated both Cat S expression and activity in AMs of wild-type mice, but only inhibited its activity in AMs of PPARγ knockout mice, pointing to a PPARγ-independent mechanism of enzyme inhibition. We hypothesized that this mechanism was electrophilic S-alkylation of target Cat S cysteines, and found that NFAs bind directly to Cat S following treatment of intact AMs and, as suggested by in silico modeling and calculation of relevant parameters, elicit S-alkylation of Cys25 when incubated with purified Cat S. These results demonstrate that NFAs' electrophilic activity, in addition to their role as PPARγ agonists, underlies their protective effects in chronic obstructive pulmonary disease (COPD) and support their therapeutic potential in this disease

DETERMINATION OF DIAGNOSTIC SIGNIFICANCE OF WRIST CIRCUMETRIC SIGN IN SIDDHA SYSTEM OF MEDICINE

Aim: Determination of diagnostic significance of wrist circumetric sign in siddha system of medicine.Materials and methods: Patients are randomly selected between the age 13years to 70 years with selected diagnoses like Madhumegam, Athikuruthiazhutham (Hypertension), Thamaraganoi, Putrunoi, Kalanjagapadai (Psoriasis), Moolam, Powthiram (Perianal fistula), Vali azhal keel vayu, Pakkavatham, Karupaisathaikatti (Uterine fibroids).Results and observation: Most of the patients MKN values fell within the range of 9-9¾ fb. This range of 9-9¾ fb MKN, was observed in Athikuruthiazhutham, Putrunoi, Moolam, Powthiram, Thamaraganoi, Madhumegam, Pakkavatham, healthy volunteers. 8-8¾fb MKN range was observed in 41.5% of Kalanjagapadai and vali azhal keel vayu cases. 10 - 10¾fb was observed in 43.1 % of patients with Karuppaisathaikattigal (Uterine fibroids).Conclusion: Since all other diseases had non-specific Manikkadai nool range except the condition Karupaisathaikattigal (uterine fibroid), Manikkadai nool can be used to assess screening test in detecting the presence of uterine fibroids and cardiac diseases according to this study.

Chikungunya Disease: Infection-Associated Markers from the Acute to the Chronic Phase of Arbovirus-Induced Arthralgia

At the end of 2005, an outbreak of fever associated with joint pain occurred in La Réunion. The causal agent, chikungunya virus (CHIKV), has been known for 50 years and could thus be readily identified. This arbovirus is present worldwide, particularly in India, but also in Europe, with new variants returning to Africa. In humans, it causes a disease characterized by a typical acute infection, sometimes followed by persistent arthralgia and myalgia lasting months or years. Investigations in the La Réunion cohort and studies in a macaque model of chikungunya implicated monocytes-macrophages in viral persistence. In this Review, we consider the relationship between CHIKV and the immune response and discuss predictive factors for chronic arthralgia and myalgia by providing an overview of current knowledge on chikungunya pathogenesis. Comparisons of data from animal models of the acute and chronic phases of infection, and data from clinical series, provide information about the mechanisms of CHIKV infection–associated inflammation, viral persistence in monocytes-macrophages, and their link to chronic signs

Air pollution from household solid fuel combustion in India: an overview of exposure and health related information to inform health research priorities

Environmental and occupational risk factors contribute to nearly 40% of the national burden of disease in India, with air pollution in the indoor and outdoor environment ranking amongst leading risk factors. It is now recognized that the health burden from air pollution exposures that primarily occur in the rural indoors, from pollutants released during the incomplete combustion of solid fuels in households, may rival or even exceed the burden attributable to urban outdoor exposures. Few environmental epidemiological efforts have been devoted to this setting, however. We provide an overview of important available information on exposures and health effects related to household solid fuel use in India, with a view to inform health research priorities for household air pollution and facilitate being able to address air pollution within an integrated rural–urban framework in the future

Modeling and Analysis of the Molecular Basis of Pain in Sensory Neurons

Intracellular calcium dynamics are critical to cellular functions like pain transmission. Extracellular ATP plays an important role in modulating intracellular calcium levels by interacting with the P2 family of surface receptors. In this study, we developed a mechanistic mathematical model of ATP-induced P2 mediated calcium signaling in archetype sensory neurons. The model architecture, which described 90 species connected by 162 interactions, was formulated by aggregating disparate molecular modules from literature. Unlike previous models, only mass action kinetics were used to describe the rate of molecular interactions. Thus, the majority of the 252 unknown model parameters were either association, dissociation or catalytic rate constants. Model parameters were estimated from nine independent data sets taken from multiple laboratories. The training data consisted of both dynamic and steady-state measurements. However, because of the complexity of the calcium network, we were unable to estimate unique model parameters. Instead, we estimated a family or ensemble of probable parameter sets using a multi-objective thermal ensemble method. Each member of the ensemble met an error criterion and was located along or near the optimal trade-off surface between the individual training data sets. The model quantitatively reproduced experimental measurements from dorsal root ganglion neurons as a function of extracellular ATP forcing. Hypothesized architecture linking phosphoinositide regulation with P2X receptor activity explained the inhibition of P2X-mediated current flow by activated metabotropic P2Y receptors. Sensitivity analysis using individual and the whole system outputs suggested which molecular subsystems were most important following P2 activation. Taken together, modeling and analysis of ATP-induced P2 mediated calcium signaling generated qualitative insight into the critical interactions controlling ATP induced calcium dynamics. Understanding these critical interactions may prove useful for the design of the next generation of molecular pain management strategies

Danger- and pathogen-associated molecular patterns recognition by pattern-recognition receptors and ion channels of the transient receptor potential family triggers the inflammasome activation in immune cells and sensory neurons.

An increasing number of studies show that the activation of the innate immune system and inflammatory mechanisms play an important role in the pathogenesis of numerous diseases. The innate immune system is present in almost all multicellular organisms and its activation occurs in response to pathogens or tissue injury via pattern-recognition receptors (PRRs) that recognize pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs). Intracellular pathways, linking immune and inflammatory response to ion channel expression and function, have been recently identified. Among ion channels, the transient receptor potential (TRP) channels are a major family of non-selective cation-permeable channels that function as polymodal cellular sensors involved in many physiological and pathological processes.In this review, we summarize current knowledge of interactions between immune cells and PRRs and ion channels of TRP families with PAMPs and DAMPs to provide new insights into the pathogenesis of inflammatory diseases. TRP channels have been found to interfere with innate immunity via both nuclear factor-kB and procaspase-1 activation to generate the mature caspase-1 that cleaves pro-interleukin-1ß cytokine into the mature interleukin-1ß.Sensory neurons are also adapted to recognize dangers by virtue of their sensitivity to intense mechanical, thermal and irritant chemical stimuli. As immune cells, they possess many of the same molecular recognition pathways for danger. Thus, they express PRRs including Toll-like receptors 3, 4, 7, and 9, and stimulation by Toll-like receptor ligands leads to induction of inward currents and sensitization in TRPs. In addition, the expression of inflammasomes in neurons and the involvement of TRPs in central nervous system diseases strongly support a role of TRPs in inflammasome-mediated neurodegenerative pathologies. This field is still at its beginning and further studies may be required.Overall, these studies highlight the therapeutic potential of targeting the inflammasomes in proinflammatory, autoinflammatory and metabolic disorders associated with undesirable activation of the inflammasome by using specific TRP antagonists, anti-human TRP monoclonal antibody or different molecules able to abrogate the TRP channel-mediated inflammatory signals