Trace element accumulation in seagrasses from the Croatian part of the Adriatic Sea

U radu je istražena raspodjela elemenata u tragovima (Al, As, Ba, Bi, Cd, Co, Cr, Cs, Cu, Fe, Li, Ni, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Ti, Tl, U, V i Zn), glavnih elemenata (K i Mg), i elemenata rijetkih zemalja (La, Ce, Pr, Nc, Sm, Eu, Gd, Tb, Dy, Ho, ER, Tm, Yb, Lu i Y) u morskim cvjetnicama (Posidonia oceanica (Linnaeus) Delile, Cymodocea nodosa (Ucria) Ascherson i Zostera noltei Hornemann) u hrvatskom dijelu Jadrana. Istovremeno, određen je sadržaj navedenih elemenata u sedimentu, kao i koncentracije Cu, Zn, Cd, Pb, Co i Ni u pridnenom vodenom sloju istraženih područja. Metodom sekvencijalne ekstrakcije procijenjen je udio elemenata u sedimentu raspoloživ za unos u cvjetnice. Utvrđeno je da je sadržaj elemenata Cd, Co, Cu, Ni, Pb i Zn u cvjetnicama nekoliko puta viši od sadržaja navedenih elemenata u vodenom stupcu, što upućuje na dobra bioakumulacijska svojstva cvjetnica. Povišene vrijednosti masenih udjela As, Cd, Co, Cu, K, Mo, Ni, Sb, Rb, U i Zn u korijenju vrste P. oceanica, u odnosu na njihove masene udjele u sedimentu, potvrđuju dobar kapacitet bioakumulacije navedenih elemenata u tkivu cvjetnice. Korelacije između sadržaja Al, Ba, Bi, Co, Cr, Cs, Cu, Fe, Li, Mn, Tl, Zn i ERZ u korijenju i sedimentu, te korelacije između sadržaja Al, Bi, Cr, Cs, Fe, Ni i Ti u rizomima cvjetnice i njihovog sadržaja u sedimentu, upućuju na mogućnost primjene vrste P. oceanica kao biološkog pokazatelja stanja sedimenta u odnosu na sadržaj navedenih elemenata. Ustanovljene razlike u raspodjeli akumuliranih elemenata u tragovima u pojedinim organima cvjetnica upućuju na različite mehanizme unosa elemenata u organizam biljke. Ovo istraživanje pruža prve opsežne podatke o sadržaju i raspodjeli elemenata u cvjetnicama u obalnom području istočnog Jadrana. Dobivena saznanja o mogućnosti primjene morskih cvjetnica kao bioindikatora onečišćenja elementima u tragovima od posebne su važnosti za priobalna područja u kojima drugi, sesilni, bioindikatorski organizmi, npr. dagnje, nisu dostupni.Distribution of trace elements (Al, As, Ba, Bi, Cd, Co, Cr, Cs, Cu, Fe, Li, Ni, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Ti, Tl, U, V, and Zn), major elements (K and Mg), and rare earth elements (La, Ce, Pr, Nc, Sm, Eu, Gd, Tb, Dy, Ho, ER, Tm, Yb, Lu, and Y) was investigated in three different seagrass species (Posidonia oceanica (Linnaeus) Delile, Cymodocea nodosa (Ucria) Ascherson, and Zostera noltei Hornemann) in the area of the Croatian Adriatic coast. In parallel with measurements of trace element content in seagrasses, analyses of trace element levels in sediment and near–bottom layer of seawater were carried out. Sequential extraction method was used to evaluate the bioavailable fraction of elements in sediments. The levels of Cd, Co, Cu, Ni, Pb and Zn in seagrasses were found to be several orders of magnitude higher compared to the levels in the surrounding water column which points to a high metal bioaccumulative capacity of seagrasses. Significant positive correlations between the levels of Al, Ba, Bi, Co, Cr, Cs, Cu, Fe, Li, Mn, Tl, Zn and ERZ in seagrass roots and sediment, as well as positive correlations between the levels of Al, Bi, Cr, Cs, Fe, Ni and Ti in rhizomes and sediment, indicate that P. oceanica may be used as a bioindicator of trace elements in sediment. The obtained results provide novel information on the level and distribution of a broad set of elements in the marine seagrasses growing in the coastal area of the eastern Adriatic. Moreover, the study will provide valuable insight on bioindicator properties of seagrasses. The obtained information on the potential use of marine seagrasses as bioindicators of pollution is important for coastal areas where other, sessile bioindicator species, e.g. mussels, are not available

Trace element accumulation in seagrasses from the Croatian part of the Adriatic Sea

U radu je istražena raspodjela elemenata u tragovima (Al, As, Ba, Bi, Cd, Co, Cr, Cs, Cu, Fe, Li, Ni, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Ti, Tl, U, V i Zn), glavnih elemenata (K i Mg), i elemenata rijetkih zemalja (La, Ce, Pr, Nc, Sm, Eu, Gd, Tb, Dy, Ho, ER, Tm, Yb, Lu i Y) u morskim cvjetnicama (Posidonia oceanica (Linnaeus) Delile, Cymodocea nodosa (Ucria) Ascherson i Zostera noltei Hornemann) u hrvatskom dijelu Jadrana. Istovremeno, određen je sadržaj navedenih elemenata u sedimentu, kao i koncentracije Cu, Zn, Cd, Pb, Co i Ni u pridnenom vodenom sloju istraženih područja. Metodom sekvencijalne ekstrakcije procijenjen je udio elemenata u sedimentu raspoloživ za unos u cvjetnice. Utvrđeno je da je sadržaj elemenata Cd, Co, Cu, Ni, Pb i Zn u cvjetnicama nekoliko puta viši od sadržaja navedenih elemenata u vodenom stupcu, što upućuje na dobra bioakumulacijska svojstva cvjetnica. Povišene vrijednosti masenih udjela As, Cd, Co, Cu, K, Mo, Ni, Sb, Rb, U i Zn u korijenju vrste P. oceanica, u odnosu na njihove masene udjele u sedimentu, potvrđuju dobar kapacitet bioakumulacije navedenih elemenata u tkivu cvjetnice. Korelacije između sadržaja Al, Ba, Bi, Co, Cr, Cs, Cu, Fe, Li, Mn, Tl, Zn i ERZ u korijenju i sedimentu, te korelacije između sadržaja Al, Bi, Cr, Cs, Fe, Ni i Ti u rizomima cvjetnice i njihovog sadržaja u sedimentu, upućuju na mogućnost primjene vrste P. oceanica kao biološkog pokazatelja stanja sedimenta u odnosu na sadržaj navedenih elemenata. Ustanovljene razlike u raspodjeli akumuliranih elemenata u tragovima u pojedinim organima cvjetnica upućuju na različite mehanizme unosa elemenata u organizam biljke. Ovo istraživanje pruža prve opsežne podatke o sadržaju i raspodjeli elemenata u cvjetnicama u obalnom području istočnog Jadrana. Dobivena saznanja o mogućnosti primjene morskih cvjetnica kao bioindikatora onečišćenja elementima u tragovima od posebne su važnosti za priobalna područja u kojima drugi, sesilni, bioindikatorski organizmi, npr. dagnje, nisu dostupni.Distribution of trace elements (Al, As, Ba, Bi, Cd, Co, Cr, Cs, Cu, Fe, Li, Ni, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Ti, Tl, U, V, and Zn), major elements (K and Mg), and rare earth elements (La, Ce, Pr, Nc, Sm, Eu, Gd, Tb, Dy, Ho, ER, Tm, Yb, Lu, and Y) was investigated in three different seagrass species (Posidonia oceanica (Linnaeus) Delile, Cymodocea nodosa (Ucria) Ascherson, and Zostera noltei Hornemann) in the area of the Croatian Adriatic coast. In parallel with measurements of trace element content in seagrasses, analyses of trace element levels in sediment and near–bottom layer of seawater were carried out. Sequential extraction method was used to evaluate the bioavailable fraction of elements in sediments. The levels of Cd, Co, Cu, Ni, Pb and Zn in seagrasses were found to be several orders of magnitude higher compared to the levels in the surrounding water column which points to a high metal bioaccumulative capacity of seagrasses. Significant positive correlations between the levels of Al, Ba, Bi, Co, Cr, Cs, Cu, Fe, Li, Mn, Tl, Zn and ERZ in seagrass roots and sediment, as well as positive correlations between the levels of Al, Bi, Cr, Cs, Fe, Ni and Ti in rhizomes and sediment, indicate that P. oceanica may be used as a bioindicator of trace elements in sediment. The obtained results provide novel information on the level and distribution of a broad set of elements in the marine seagrasses growing in the coastal area of the eastern Adriatic. Moreover, the study will provide valuable insight on bioindicator properties of seagrasses. The obtained information on the potential use of marine seagrasses as bioindicators of pollution is important for coastal areas where other, sessile bioindicator species, e.g. mussels, are not available

Supplementary data for article: Vasić, V.; Gašić, U.; Stanković, D.; Lušić, D.; Vukić-Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Towards Better Quality Criteria of European Honeydew Honey: Phenolic Profile and Antioxidant Capacity. Food Chemistry 2019, 274, 629–641. https://doi.org/10.1016/j.foodchem.2018.09.045

Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2917]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2235]Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S0308814618316169?via%3Dihub

Supplementary data for article: Vasić, V.; Gašić, U.; Stanković, D.; Lušić, D.; Vukić-Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Towards Better Quality Criteria of European Honeydew Honey: Phenolic Profile and Antioxidant Capacity. Food Chemistry 2019, 274, 629–641. https://doi.org/10.1016/j.foodchem.2018.09.045

Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2917]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2235]Supplementary material for: [https://www.sciencedirect.com/science/article/pii/S0308814618316169?via%3Dihub

Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457

Supplementary material for: [https://doi.org/10.1016/j.foodchem.2019.125457]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3462

Supplementary data for article: Vasić, V.; Đurđić, S.; Tosti, T.; Radoičić, A.; Lušić, D.; Milojković-Opsenica, D.; Tešić, Ž.; Trifković, J. Two Aspects of Honeydew Honey Authenticity: Application of Advance Analytical Methods and Chemometrics. Food Chem. 2020, 305. https://doi.org/10.1016/j.foodchem.2019.125457

Supplementary material for: [https://doi.org/10.1016/j.foodchem.2019.125457]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3462

Marine chemical contaminants – support to the harmonization of MSFD D8 methodological standards: Matrices and threshold values/reference levels for relevant substances

According to the Article 17(2) of the Marine Strategy Framework Directive (MSFD), Member States have to review and update their marine strategies every six years. This requires updates of the MSFD Articles 8, 9 and 10 by 2018. The current report provides an overview of the substances, matrices and threshold values that Member States intend to use for the assessment of the Descriptor 8 in this MSFD reporting cycle. This compilation aims at evaluating gaps and discrepancies between Member States and identifying aspects that need further harmonization. It also helps understand which issues should be addressed to achieve consistency with the new MSFD Commission Decision (EU 2017/848). The information has been gathered from the contributions of the MSFD Expert Network on Contaminants, an informal network established to support MSFD implementation. This work is part of a process to help regulators to assess relevant contaminants in their jurisdictional area, thus aiming at EU national authorities but also at Regional Sea Conventions in the shared marine basins.JRC.D.2-Water and Marine Resource

Water for all : Proceedings of the 7th international scientific and professional conference Water for all

The 7th International Scientific and Professional Conference Water for all is organized to honour the World Water Day by the Josip Juraj Strossmayer University of Osijek, European Hygienic Engineering & Design Group (EHEDG), Danube Parks, Croatian Food Agency, Croatian Water, Faculty of Food Technology Osijek, Faculty of Agriculture in Osijek, Faculty of Civil Engineering Osijek, Josip Juraj Strossmayer University of Osijek Department of Biology, Josip Juraj Strossmayer University of Osijek Department of Chemistry, Nature Park “Kopački rit”, Osijek- Baranja County, Public Health Institute of the Osijek- Baranja County and „Vodovod-Osijek“ -water supply company in Osijek. The topic of World Water Day 2017 was "Wastewater" emphasizing the importance and influence of wastewater treatments on global environment. The international scientific and professional conference Water for all is a gathering of scientists and experts in the field of water management, including chemists, biologists, civil and agriculture engineers, with a goal to remind people about the significance of fresh water and to promote an interdisciplinary approach and sustainability for fresh water resource management. The Conference has been held since 2011. About 300 scientists and engineers submitted 95 abstracts to the 7th International Scientific and Professional Conference Water for all, out of which 33 was presented orally and 62 as posters. 47 full papers were accepted by the Scientific Committee. 38 full papers became the part of the this Proceedings while 9 papers were accepted for publication in Croatian Journal of Food Science and Technology and Electronic Journal of the Faculty of Civil Engineering Osijek - e-GFOS

Trace element accumulation in seagrasses from the Croatian part of the Adriatic Sea

U radu je istražena raspodjela elemenata u tragovima (Al, As, Ba, Bi, Cd, Co, Cr, Cs, Cu, Fe, Li, Ni, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Ti, Tl, U, V i Zn), glavnih elemenata (K i Mg), i elemenata rijetkih zemalja (La, Ce, Pr, Nc, Sm, Eu, Gd, Tb, Dy, Ho, ER, Tm, Yb, Lu i Y) u morskim cvjetnicama (Posidonia oceanica (Linnaeus) Delile, Cymodocea nodosa (Ucria) Ascherson i Zostera noltei Hornemann) u hrvatskom dijelu Jadrana. Istovremeno, određen je sadržaj navedenih elemenata u sedimentu, kao i koncentracije Cu, Zn, Cd, Pb, Co i Ni u pridnenom vodenom sloju istraženih područja. Metodom sekvencijalne ekstrakcije procijenjen je udio elemenata u sedimentu raspoloživ za unos u cvjetnice. Utvrđeno je da je sadržaj elemenata Cd, Co, Cu, Ni, Pb i Zn u cvjetnicama nekoliko puta viši od sadržaja navedenih elemenata u vodenom stupcu, što upućuje na dobra bioakumulacijska svojstva cvjetnica. Povišene vrijednosti masenih udjela As, Cd, Co, Cu, K, Mo, Ni, Sb, Rb, U i Zn u korijenju vrste P. oceanica, u odnosu na njihove masene udjele u sedimentu, potvrđuju dobar kapacitet bioakumulacije navedenih elemenata u tkivu cvjetnice. Korelacije između sadržaja Al, Ba, Bi, Co, Cr, Cs, Cu, Fe, Li, Mn, Tl, Zn i ERZ u korijenju i sedimentu, te korelacije između sadržaja Al, Bi, Cr, Cs, Fe, Ni i Ti u rizomima cvjetnice i njihovog sadržaja u sedimentu, upućuju na mogućnost primjene vrste P. oceanica kao biološkog pokazatelja stanja sedimenta u odnosu na sadržaj navedenih elemenata. Ustanovljene razlike u raspodjeli akumuliranih elemenata u tragovima u pojedinim organima cvjetnica upućuju na različite mehanizme unosa elemenata u organizam biljke. Ovo istraživanje pruža prve opsežne podatke o sadržaju i raspodjeli elemenata u cvjetnicama u obalnom području istočnog Jadrana. Dobivena saznanja o mogućnosti primjene morskih cvjetnica kao bioindikatora onečišćenja elementima u tragovima od posebne su važnosti za priobalna područja u kojima drugi, sesilni, bioindikatorski organizmi, npr. dagnje, nisu dostupni.Distribution of trace elements (Al, As, Ba, Bi, Cd, Co, Cr, Cs, Cu, Fe, Li, Ni, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Ti, Tl, U, V, and Zn), major elements (K and Mg), and rare earth elements (La, Ce, Pr, Nc, Sm, Eu, Gd, Tb, Dy, Ho, ER, Tm, Yb, Lu, and Y) was investigated in three different seagrass species (Posidonia oceanica (Linnaeus) Delile, Cymodocea nodosa (Ucria) Ascherson, and Zostera noltei Hornemann) in the area of the Croatian Adriatic coast. In parallel with measurements of trace element content in seagrasses, analyses of trace element levels in sediment and near–bottom layer of seawater were carried out. Sequential extraction method was used to evaluate the bioavailable fraction of elements in sediments. The levels of Cd, Co, Cu, Ni, Pb and Zn in seagrasses were found to be several orders of magnitude higher compared to the levels in the surrounding water column which points to a high metal bioaccumulative capacity of seagrasses. Significant positive correlations between the levels of Al, Ba, Bi, Co, Cr, Cs, Cu, Fe, Li, Mn, Tl, Zn and ERZ in seagrass roots and sediment, as well as positive correlations between the levels of Al, Bi, Cr, Cs, Fe, Ni and Ti in rhizomes and sediment, indicate that P. oceanica may be used as a bioindicator of trace elements in sediment. The obtained results provide novel information on the level and distribution of a broad set of elements in the marine seagrasses growing in the coastal area of the eastern Adriatic. Moreover, the study will provide valuable insight on bioindicator properties of seagrasses. The obtained information on the potential use of marine seagrasses as bioindicators of pollution is important for coastal areas where other, sessile bioindicator species, e.g. mussels, are not available

Analysis of Manganese Bioaccumulated in Mediterranean Blue Mussel (Mytilus galloprovincialis) from the Bay of Mali Ston (Adriatic Sea, Croatia) during Diarrhetic Shellfish Poisoning Toxicity

Diarrhetic Shellfish Poisoning (DSP) toxicity was revealed in the Mediterranean blue mussel (Mytilus galloprovincialis) from the Bay of Mali Ston, in the south part of the Eastern Adriatic Sea, through the Croatian National Monitoring Programme in the period from January until June of 2011. A survey of DSP toxicity within the frame of regular controls carried out through the mouse bioassay (MBA, at the time the official method for DSP toxins) demonstrated that in some incidents, positive MBA, which manifested by the atypical symptomatology of the animals, dominated. Additional studies were done to explain the atypical results of the conducted biological tests at the time. In the current study, the bioaccumulated manganese concentration in the soft tissues of mussels was measured to investigate its influence on the MBA results. In both DSP negative and DSP positive samples, which were prepared for the analysis according to the modified US EPA 3052 method, the concentration of the bioaccumulated manganese was performed on the atomic absorption spectrometer using flame atomic absorption spectroscopy technique. The analysis revealed higher concentration of manganese in 87% of DSP positive samples and the expressed per wet weight ranged from 0.15 to 5.38 mg kg−1. The mean concentration of manganese for all DSP positive samples was 1.78 mg kg−1, while for DSP negative samples, it was 48% lower (0.93 mg kg−1). The highest concentration of manganese in DSP positive samples was measured in February 2011. Since the low concentrations of lipophilic biotoxins gymnodimines (GYMs) and spirolides (SPXs) were also detected in the analysed DSP positive samples in the parallel studies, the results obtained in this study suggest future investigations of the connection between the concentration of manganese and lipophilic biotoxins