Variabilidade genética de Xanthomonas axonopodis pv. passiflorae

A mancha bacteriana, causada por Xanthomonas axonopodis pv. passiflorae, é uma das mais importantes doenças do maracujazeiro, podendo limitar a produção dessa frutífera em algumas regiões do País. O uso de resistência genética e controle químico, juntamente com o emprego de medidas de exclusão, são as práticas de controle da doença mais recomendadas. Para o desenvolvimento de variedades resistentes é necessário conhecer tanto a variabilidade genética do hospedeiro quanto do patógeno. Nesse trabalho foi estudada a variabilidade de cinqüenta isolados patogênicos de Xanthomonas axonopodis pv. passiflorae, coletados em quatro diferentes locais no estado de São Paulo. No estudo da variabilidade genética foram usados dados de marcadores moleculares RAPD (Random Amplified Polymorphic DNA), os quais foram usados para o cálculo do coeficiente de similaridade de Dice entre os isolados, análise de variância molecular (AMOVA) entre e dentro das populações e agrupamento dos isolados pelo método UPGMA. Para análise da agressividade foram usados cinco isolados, mais divergentes, baseado no dendrograma. O coeficiente de similaridade variou entre 0,6887 e 0,9688. Na análise de agrupamento, os isolados foram separados em sete grupos e não houve relação evidente entre local de coleta com a composição dos grupos. Na análise da variância molecular (AMOVA) verificou-se que a maior parte da variabilidade genética está dentro das populações (89,4%) e apenas 10,6%, entre populações. Os resultados da análise de agrupamento e da AMOVA indicam que existe grande fluxo gênico entre isolados bacterianos nas regiões analisadas. No teste de patogenicidade verificou-se diferença significativa de agressividade entre os isolados. Os resultados demonstram a importância do conhecimento da variabilidade genética e da agressividade na seleção dos isolados para serem utilizados em testes de resistência genética no desenvolvimento de variedades resistentes.The bacterial spot, caused by Xanthomonas axonopodis pv. passiflorae , is one of the most important diseases of passion fruit, which can drastically limit the production in some regions of Brazil. The use of genetic resistance, chemical control and the adoption of exclusion measures are the more recommended practices for the control of this disease. To produce the resistant varieties knowledge about the variability of both host and pathogen is needed. In this study, we investigated the variability of 50 pathogenic isolates of X. axonopodis pv. passiflorae, collected from four production areas in São Paulo State. We used RAPD markers (Random Amplified Polymorphic DNA) for identification of the degree of genetic similarities through dice coefficient, analysis of molecular variance (AMOVA), among as well as within populations and clustering by the UPGMA method. Based on the clustering, the five more divergent isolates were selected for agressiveness trials. The similarity coefficient ranged from 0.6887 to 0.9688. Clustering analysis grouped the isolates in seven categories and neither apparent correlation was identified among their geographical origin no clustering pattern. The AMOVA data showed that the variability within and among populations were 89.4% and 10.6% respectively. The clustering analysis indicated genic flow among the different production areas. The results highlights the importance of taking into consideration the bacterial genetic variability of this bacterium as well as its agressiveness during the selection of resistant varieties

Fontes não convencionais de fósforo para gado zebu no Brasil

To evaluate non-conventional phosphorus sources, a group of 400 Nellore cows mantained under pasture received a mineral mixture with different P sources (group I: superphosphate - 500 ppm P; group II: superphosphate - 340 ppm P plus rock phosphate Patos 160 ppm P; group III: superphosphate 340 ppm P plus dicalcium phosphate 160 ppm P; group IV - dicalcium phosphate 500 ppm P). There were no differences in pregnancy rate, calving rate and calving interval. A fluorine deposition in bone was observed for the treatments with superphosphate and rock phosphate (66.92 ± 15.53; 69.97 ± 6.5 and 64.05 ± 3.35% respectively for group I, II and III). Superphosphate was almost as good dicalcium phosphate to provide phosphorus for grazing cows and there was a potentially significant economic advantage over dicalcium phosphate.Quatrocentas vacas Nelore, mantidas em pastagem, receberam mistura mineral contendo fontes não convencionais de fósforo (grupo I - 500 ppm P-superfosfato triplo; grupo II - 340 ppm P como superfosfato e 160 ppm P como rocha Patos; grupo III - 340 ppm P como superfosfato e 160 ppm P como fosfato bicálcico; grupo IV -500 ppm P como fosfato bicálcico). Não se verificou diferenças na taxa de prenhez, porcentagem de nascimento e intervalo entre partos. Observou-se que houve maior concentração de flúor nos ossos para o tratamento com fosfato de rocha. O superfosfato triplo apresentou-se como fonte adequada de P e mostrou vantagem econômica em relação ao bicálcico

Presubiculum stimulation in vivo evokes distinct oscillations in superficial and deep entorhinal cortex layers in chronic epileptic rats

The characteristic cell loss in layer III of the medial entorhinal area (MEA-III) in human mesial temporal lobe epilepsy is reproduced in the rat kainate model of the disease. To understand how this cell loss affects the functional properties of the MEA, we investigated whether projections from the presubiculum (prS), providing a main input to the MEA-III, are altered in this epileptic rat model. Injections of an anterograde tracer in the prS revealed bilateral projection fibers mainly to the MEA-III in both control and chronic epileptic rats. We further examined the prS-MEA circuitry using a 16-channel electrode probe covering the MEA in anesthetized control and chronic epileptic rats. With a second 16-channel probe, we recorded signals in the hippocampus. Current source density analysis indicated that, after prS double-pulse stimulation, afterdischarges in the form of oscillations (20-45 Hz) occurred that were confined to the superficial layers of the MEA in all epileptic rats displaying MEA-III neuronal loss. Slower oscillations (theta range) were occasionally observed in the deep MEA layers and the dentate gyrus. This kind of oscillation was never observed in control rats. We conclude that dynamical changes occur in an extensive network within the temporal lobe in epileptic rats, manifested as different kinds of oscillations, the characteristics of which depend on local properties of particular subareas. These findings emphasize the significance of the entorhinal cortex in temporal lobe epilepsy and suggest that the superficial cell layers could play an important role in distributing oscillatory activity.status: publishe

Stringy cosmic strings in matter coupled N=2, d=4 supergravity

We extend the system of ungauged N=2, d=4 supergravity coupled to vector multiplets and hypermultiplets with 2-form potentials. The maximal number of 2-form potentials that one may introduce is equal to the number of isometries of either the special Kaehler or quaternionic Kaehler sigma model. We show that the local supersymmetry algebra can be realized on the 2-form potentials. These 2-forms couple electrically to strings which we refer to as stringy cosmic strings. The 1/2 BPS bosonic world-sheet actions for these strings are constructed and we discuss the properties of the 1/2 BPS stringy cosmic string solutions.Comment: 36 pages, added references, corrected typos, modificated Appendix

Benefícios da aplicação de azoto, potássio e boro na fisiologia da oliveira em condições de sequeiro

O azoto, o potássio e o boro são nutrientes que frequentemente condicionam a fisiologia da oliveira (Olea europaea L.). O azoto apresenta um papel primordial por ser constituinte de vários compostos orgânicos, em especial proteínas, ácidos nucleicos, hormonas e clorofila. O boro influencia directamente a produção por proporcionar maior desenvolvimento radicular, maximizando o aproveitamento da água e dos fertilizantes, bem como por estar directamente envolvido na formação da parede celular, contribuindo para aumento da resistência geral da planta. Relativamente ao potássio, para além do efeito directo na produção, existem evidências que as árvores bem nutridas com este elemento toleram melhor a seca. Neste trabalho apresentam-se resultados de um ensaio de campo com duas modalidades de azoto, duas modalidades de potássio e duas modalidades de boto, conduzidos em olival de sequeiro de Verdeal Transmontana em Mirandela, desde o início de 2004

Special Geometry of Euclidean Supersymmetry III: the local r-map, instantons and black holes

We define and study projective special para-Kahler manifolds and show that they appear as target manifolds when reducing five-dimensional vector multiplets coupled to supergravity with respect to time. The dimensional reductions with respect to time and space are carried out in a uniform way using an epsilon-complex notation. We explain the relation of our formalism to other formalisms of special geometry used in the literature. In the second part of the paper we investigate instanton solutions and their dimensional lifting to black holes. We show that the instanton action, which can be defined after dualising axions into tensor fields, agrees with the ADM mass of the corresponding black hole. The relation between actions via Wick rotation, Hodge dualisation and analytic continuation of axions is discussed.Comment: 72 pages, 2 figure

Tracking Quintessence and Cold Dark Matter Candidates

We study the generation of a kination-dominated phase in the context of a quintessential model with an inverse-power-law potential and a Hubble-induced mass term for the quintessence field. The presence of kination is associated with an oscillating evolution of the quintessence field and the barotropic index. We find that, in sizeable regions of the parameter space, a tracker scaling solution can be reached sufficiently early to alleviate the coincidence problem. Other observational constraints originating from nucleosynthesis, the inflationary scale, the present acceleration of the universe and the dark-energy-density parameter can be also met. The impact of this modified kination-dominated phase on the thermal abundance of cold dark matter candidates is investigated too. We find that: (i) the enhancement of the relic abundance of the WIMPs with respect to the standard paradigm, crucially depends on the hierarchy between the freeze-out temperature and the temperature at which the extrema in the evolution of the quintessence field are encountered, and (ii) the relic abundance of e-WIMPs takes its present value close to the temperature at which the earliest extremum of the evolution of the quintessence field occurs and, as a consequence, both gravitinos and axinos arise as natural cold dark matter candidates. In the case of unstable gravitinos, the gravitino constraint can be satisfied for values of the initial temperature well above those required in the standard cosmology.Comment: Final versio