49 research outputs found
2′-Fluoro-4′-thioarabino-modified oligonucleotides: conformational switches linked to siRNA activity
The synthesis of oligonucleotides containing 2′-deoxy-2′-fluoro-4′-thioarabinonucleotides is described. 2′-Deoxy-2′-fluoro-5-methyl-4′-thioarabinouridine (4′S-FMAU) was incorporated into 18-mer antisense oligonucleotides (AONs). 4′S-FMAU adopts a predominantly northern sugar conformation. Oligonucleotides containing 4′S-FMAU, unlike those containing FMAU, were unable to elicit E. coli or human RNase H activity, thus corroborating the hypothesis that RNase H prefers duplexes containing oligonucleotides that can adopt eastern conformations in the antisense strand. The duplex structure and stability of these oligonucleotides was also investigated via circular dichroism (CD)- and UV- binding studies. Replacement of the 4′-oxygen by a sulfur atom resulted in a marked decrease in melting temperature of AON:RNA as well as AON:DNA duplexes. 2′-Deoxy-2′-fluoro-4′-thioarabinouridine (4′S-FAU) was incorporated into 21-mer small interfering RNA (siRNA) and the resulting siRNA molecules were able to trigger RNA interference with good efficiency. Positional effects were explored, and synergy with 2′F-ANA, which has been previously established as a functional siRNA modification, was demonstrated
Prédétermination d'une préférence alimentaire par stimulation olfactive post-partum. Premiers résultats chez la ratte
International audienc
In vitro mouse model in Duchenne muscular dystrophy diagnosis using 50-MHz ultrasound waves
International audienc
Comparison of the capsaicin- and amino acid-sensitivity of dorsal root C fibres in the rat and the toad
Application of time resolved x-ray diffraction to study the solidification of glass-former melts.
Application of time resolved x-ray diffraction to study the solidification of glass-former melts.
Synergism by Coassembly at the Origin of Ion Selectivity in Liquid–Liquid Extraction
International audienc