A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression.

Brn3b (Pou4f2) is a class-4 POU domain transcription factor known to play central roles in the development of different neuronal populations of the Central Nervous System, including retinal ganglion cells (RGCs), the neurons that connect the retina with the visual centers of the brain. Here, we have used CRISPR-based genetic engineering to generate a Brn3b-mCherry reporter mouse without altering the endogenous expression of Brn3b. In our mouse line, mCherry faithfully recapitulates normal Brn3b expression in the retina, the optic tracts, the midbrain tectum, and the trigeminal ganglia. The high sensitivity of mCherry also revealed novel expression of Brn3b in the neuroectodermal cells of the optic stalk during early stages of eye development. Importantly, the fluorescent intensity of Brn3b-mCherry in our reporter mice allows for noninvasive live imaging of RGCs using Scanning Laser Ophthalmoscopy (SLO), providing a novel tool for longitudinal monitoring of RGCs

THE INTERPLAY OF NOSTALGIA AND MEMORABLE EXPERIENCES: A STUDY ON APPAREL

This study analyzed the relationship between nostalgia and memorable experiences on con­sumer purchase intention. While nostalgia was well-researched for Generation X and Y, there was a lack of understanding of how nostalgia influenced Generation Z brands and consumption pre­ferences. Nostalgia marketing has been popular for several years, and there was literature on pro­duct and service categories, such as movie remakes, band reunions, candy, and brands that have completed their life cycle and could be re-released to the market by appealing to consumers nos­tal­gia. It used a sample of 135 observations and sneakers stimuli advertising with nostalgic com­ponents. Results showed that nostalgia affected purchase intention directly and the retrieval of memorable experiences of using the apparel product. However, it did not translate to purchase intention. The results helped marketers identify courses of action to create effective commu­ni­ca­tion that triggers consumers' purchase intention who did not require product development

NUEVO ENFOQUE DEL NEGOCIO DE LAS TARJETAS BANCARIAS EN ESPAÑA Y EL NUEVO PAPEL A DESARROLLAR POR EL COMERCIANTE

El objetivo de este trabajo es analizar los elementos básicos del nuevo enfoque de las entidades financieras españolas para su negocio de tarjetas bancarias, así como el nuevo papel que podría tener el comerciante para su desarrollo. Para ello se envió un cuestionario a 60 responsables de este negocio en dichas entidades. De las respuestas recogidas en los 44 cuestionarios recibidos, se concluye que este nuevo enfoque deberá hacer más hincapié en las operaciones de financiación al consumo mediante tarjeta bancaria. Así mismo, se verifica que el comerciante deberá tener un papel relevante y retribuido como prescriptor de estas operaciones

El periodismo ambiental como área de especialización en las aulas universitarias

Report environment issues has become in recent years an consolidated area of specialization in journalism when environment is a political, economic and social question. Journalism education can not ignore the need to know this cross curricular subject. The demands of the European Space of Higher Education in our universities requires consideration of new approaches for integrating environment in the classroom.Informar sobre los asuntos relacionados con el medio ambiente se ha convertido en los últimos años en un área de especialización periodística consolidada al mismo tiempo que la actualidad demuestra que los asuntos ambientales son asuntos políticos, económicos y sociales. La enseñanza universitaria del Periodismo no puede ser ajena a la necesidad de conocer esta realidad transversal. Las exigencias de la implantación del Espacio Europeo de Educación Superior en nuestras universidades obliga a considerar nuevas fórmulas para la integración del medio ambiente en las aulas

CULTURA ORGANIZACIONAL Y LA SATISFACCIÓN LABORAL EN LOS TRABAJADORES DE LA REGIÓN DE SANIDAD POLICIAL HUÁNUCO, 2021

La investigación formulada incluye el análisis de la relación entre la cultura empresarial y la satisfacción laboral de los trabajadores de la Región de Sanidad Policial de Huánuco, 2021, con el fin de determinar el grado de importancia de la satisfacción laboral. Por lo tanto, se procesaron de manera eficiente con los recursos más valiosos, como el tiempo y los costos asociados. En primer lugar, el objetivo general de la investigación fue si existe una conexión significativa entre la cultura organizacional y la satisfacción laboral de los trabajadores de la institución. La investigación fue de tipo aplicada, con un diseño no experimental, transversal con una muestra de 60 trabajadores que actualmente laboran en dicha institución, a los cuales se les aplicó un cuestionario en escala Likert como herramienta para las dos variables y se determinó su confiabilidad, utilizando el estadístico Alpha de Cronbach para las dos variables, que se analizó con la prueba Rho de Sperman. Los resultados obtenidos tras la realización de la encuesta mostraron que la relación entre las variables de la institución es satisfactoria, lo que subraya el alto nivel de satisfacción y buena cultura laboral entre los trabajadores. En los resultados se concluye que la cultura organizacional está relacionada significativamente con la satisfacción laboral de los trabajadores, teniendo un coeficiente de correlación de 0.597, interpretándose como una correlación positiva moderada. Además, el nivel de significancia es menor que 0.05 (p=0.001<0.05), esto indica que si existe relación entre las variables.Tesi

The quality of higher education from the point of view of the contributions made by graduates in their work environment

Objective: The objective of this paper was to analyze higher education quality, considering the contributions made by graduates in their work environment.&nbsp; The ontological context was determined by the Master's program in Education of the Caribbean University System (SUE Caribe) at the University of Sucre from Sincelejo, Colombia. Method: The study was developed within the framework of the qualitative approach, with a case study design, cross-sectional in two stages: documentary review and field research. A semi-structured interview was applied to an intentional sample of 13 graduates selected through cluster, proportional, and random sampling. Data analysis was executed using ATLAS Ti software version 7.5.4, It takes into account three levels of categories: core, relational, and descriptive. Results: the graduates interviewed are located in the educational sector, It shows good performance in the following competencies: research, educational management, and social projection, and are recognized by the community. Discussion and Conclusions: considering the graduate as the unit of analysis, the program exceeds the qualified registry requirements and meets the high-quality requisites.&nbsp

A SOCIAL MARKETING INTERVENTION MODEL TO INFLUENCE THE SOCIAL ACCEPTANCE OF NATURAL DYES, FROM DOMESTIC FOOD WASTE RESOURCES IN MEXICO WITHOUT METALLIC CHEMICAL ADDITIVES FOR TEXTILE DYEING

This research proposes a social marketing intervention model to educate and thus influence a behavioural change in Mexican females from 18 to 33 years old towards the acceptance of natural dyes from domestic food waste resources without metallic chemical additives. The foundations of this research are the water and food waste situation in Mexico. This study includes experimental work with Allium Cepa (onion) skin as an example of a Mexican food waste resource to analyse its performance as a natural dyestuff without metallic chemical additives on cotton and re-frames the design problem, challenging what has previously been considered to be suitable by the market with regards to natural dyed textiles such as colour strength and colour fastness. The study investigates the social acceptance towards natural dyed textiles with limited colour strength and colour fastness, embracing the limitations of the process as an opportunity to personalise the end garment and take ownership of the creative process, rather than using metallic chemical additives that could affect Mexico´s waterways adversely. The survey utilised to collect data, shows that for Mexican females from 18 to 33 years old the environmental impacts of metallic chemicals are more important, than the colour strength and colour fastness of a garment. This validates the hypothesis of the study, which is the following: If consumers become aware of the environmental impact that is made through the use of synthetic dyes, they eventually become more receptive to embrace the limitations of the use of natural dyes without metallic chemical additives such as lack of colour strength, standardisation and durability when compared to synthetic dyes. The novelty of this study is a social marketing intervention model in the transition design domain in which co-design was identified as the channel by which the target audience could translate their acceptance of natural dyes from domestic food waste resources without metallic chemical additives into action by becoming co-designers of their own natural dyed garments. While, DIY re-dye activity, and customisation were identified as the promotion (strategic tools) in the model that could influence the attraction of the target audience