Bacteria Use Type IV Pili to Walk Upright and Detach from Surfaces

1. Department of Bioengineering, California Nano Systems Institute,University of California, Los Angeles, CA 90024, USA. 2. Department of Chemical and Biomolecular Engineering, University of Houston, Houston, TX 77204, USA. 3. Department of Materials Science and Engineering, University of Illinois, Urbana-Champaign, IL 61801, USA. 4. Department of Civil Engineering and Geological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.Bacterial biofilms are structured multicellular communities involved in a broad range of infections. Knowing how free-swimming bacteria adapt their motility mechanisms near surfaces is crucial for understanding the transition between planktonic and biofilm phenotypes. By translating microscopy movies into searchable databases of bacterial behavior, we identified fundamental type IV pili–driven mechanisms for Pseudomonas aeruginosa surface motility involved in distinct foraging strategies. Bacteria stood upright and “walked” with trajectories optimized for two-dimensional surface exploration. Vertical orientation facilitated surface detachment and could influence biofilm morphology.Center for Nonlinear Dynamic

Cool Companions to White Dwarf Stars from the Two Micron All Sky Survey All Sky Data Release

We present the culmination of our near-infrared survey of the optically spectroscopically identified white dwarf stars from the McCook and Sion catalog, conducted using photometric data from the Two Micron All Sky Survey final All Sky Data Release. The color selection technique, which identifies candidate binaries containing a white dwarf and a low-mass stellar (or substellar) companion via their distinctive locus in the near-infrared color-color diagram, is demonstrated to be simple to apply and to yield candidates with a high rate of subsequent confirmation. We recover 105 confirmed binaries, and identify 27 firm candidates (19 of which are new to this work) and 21 tentative candidates (17 of which are new to this work) from the 2MASS data. Only a small number of candidates from our survey have likely companion spectral types later than M5, none of which is an obvious L-type (i.e., potential brown dwarf) companion. Only one previously known white dwarf + brown dwarf binary is detected. This result is discussed in the context of the 2MASS detection limits, as well as other recent observational surveys that suggest a very low rate of formation (or survival) for binary stars with extreme mass ratios

Velocity–conductivity relationships for mantle mineral assemblages in Archean cratonic lithosphere based on a review of laboratory data and Hashin–Shtrikman extremal bounds

Author Posting. © Elsevier B.V., 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Lithos 109 (2009): 131-143, doi:10.1016/j.lithos.2008.10.014.Can mineral physics and mixing theories explain field observations of seismic velocity and electrical conductivity, and is there an advantage to combining seismological and electromagnetic techniques? These two questions are at the heart of this paper. Using phenomologically-derived state equations for individual minerals coupled with multi-phase, Hashin-Shtrikman extremal-bound theory we derive the likely shear and compressional velocities and electrical conductivity at three depths, 100 km, 150 km and 200 km, beneath the central part of the Slave craton and beneath the Kimberley region of the Kaapvaal craton based on known petrologically-observed mineral abundances and magnesium numbers, combined with estimates of temperatures and pressures. We demonstrate that there are measurable differences between the physical properties of the two lithospheres for the upper depths, primarily due to the different ambient temperature, but that differences in velocity are negligibly small at 200 km. We also show that there is an advantage to combining seismic and electromagnetic data, given that conductivity is exponentially dependent on temperature whereas the shear and bulk moduli have only a linear dependence in cratonic lithospheric rocks. Focussing on a known discontinuity between harzburgite-dominated and lherzolitic mantle in the Slave craton at a depth of about 160 km, we demonstrate that the amplitude of compressional (P) wave to shear (S) wave conversions would be very weak, and so explanations for the seismological (receiver function) observations must either appeal to effects we have not considered (perhaps anisotropy), or imply that the laboratory data require further refinement

Predicting the F(ab)-mediated effect of monoclonal antibodies in vivo by combining cell-level kinetic and pharmacokinetic modelling

Cell-level kinetic models for therapeutically relevant processes increasingly benefit the early stages of drug development. Later stages of the drug development processes, however, rely on pharmacokinetic compartment models while cell-level dynamics are typically neglected. We here present a systematic approach to integrate cell-level kinetic models and pharmacokinetic compartment models. Incorporating target dynamics into pharmacokinetic models is especially useful for the development of therapeutic antibodies because their effect and pharmacokinetics are inherently interdependent. The approach is illustrated by analysing the F(ab)-mediated inhibitory effect of therapeutic antibodies targeting the epidermal growth factor receptor. We build a multi-level model for anti-EGFR antibodies by combining a systems biology model with in vitro determined parameters and a pharmacokinetic model based on in vivo pharmacokinetic data. Using this model, we investigated in silico the impact of biochemical properties of anti-EGFR antibodies on their F(ab)-mediated inhibitory effect. The multi-level model suggests that the F(ab)-mediated inhibitory effect saturates with increasing drug-receptor affinity, thereby limiting the impact of increasing antibody affinity on improving the effect. This indicates that observed differences in the therapeutic effects of high affinity antibodies in the market and in clinical development may result mainly from Fc-mediated indirect mechanisms such as antibody-dependent cell cytotoxicity

A Novel Three-Phase Model of Brain Tissue Microstructure

We propose a novel biologically constrained three-phase model of the brain microstructure. Designing a realistic model is tantamount to a packing problem, and for this reason, a number of techniques from the theory of random heterogeneous materials can be brought to bear on this problem. Our analysis strongly suggests that previously developed two-phase models in which cells are packed in the extracellular space are insufficient representations of the brain microstructure. These models either do not preserve realistic geometric and topological features of brain tissue or preserve these properties while overestimating the brain's effective diffusivity, an average measure of the underlying microstructure. In light of the highly connected nature of three-dimensional space, which limits the minimum diffusivity of biologically constrained two-phase models, we explore the previously proposed hypothesis that the extracellular matrix is an important factor that contributes to the diffusivity of brain tissue. Using accurate first-passage-time techniques, we support this hypothesis by showing that the incorporation of the extracellular matrix as the third phase of a biologically constrained model gives the reduction in the diffusion coefficient necessary for the three-phase model to be a valid representation of the brain microstructure

Экспериментальное изучение влияния глюкозамина гидрохлорида на развитие патоспермии стареющих крыс, вызванной доксорубицином

ГЛЮКОЗАМИНА ГИДРОХЛОРИДДОКСОРУБИЦИНПАТОСПЕРМИЯСТАРЕНИЕФАРМАКОЛОГИЯ КЛИНИЧЕСКАЯЛЕКАРСТВ ФИЗИОЛОГИЧЕСКОЕ ДЕЙСТВИЕРЕПРОДУКЦИЮ КОНТРОЛИРУЮЩИЕ СРЕДСТВАЭКСПЕРИМЕНТЫ НА ЖИВОТНЫХКРЫСЫЦель работы - исследование влияния глюкозамина гидрохлорида на развитие гипофункции семенников крыс, вызванной длительным введением доксорубицина на фоне старения животных

N. elongata Produces Type IV Pili That Mediate Interspecies Gene Transfer with N. gonorrhoeae

The genus Neisseria contains at least eight commensal and two pathogenic species. According to the Neisseria phylogenetic tree, commensals are basal to the pathogens. N. elongata, which is at the opposite end of the tree from N. gonorrhoeae, has been observed to be fimbriated, and these fimbriae are correlated with genetic competence in this organism. We tested the hypothesis that the fimbriae of N. elongata are Type IV pili (Tfp), and that Tfp functions in genetic competence. We provide evidence that the N. elongata fimbriae are indeed Tfp. Tfp, as well as the DNA Uptake Sequence (DUS), greatly enhance N. elongata DNA transformation. Tfp allows N. elongata to make intimate contact with N. gonorrhoeae and to mediate the transfer of antibiotic resistance markers between these two species. We conclude that Tfp functional for genetic competence is a trait of a commensal member of the Neisseria genus. Our findings provide a mechanism for the horizontal gene transfer that has been observed among Neisseria species