Live lecture screencast recording: a proposal to simplify the tasks associated with content production for video-teaching

Se trata de un resumen (Abstract) de la contribución. El acuerdo de transferencia de copyright del trabajo completo es incompatible con el depósito del mismo en RIUMA.Se describe la implementación inicial de un método simplificado de grabación en directo de sesiones de clase en asignaturas de las Escuelas de Ingeniería de la Universidad de Málaga. La simplificación se logra al incorporar en la actividad diaria del aula ciertas características típicas de la grabación en diferido de mini videos docentes. En una configuración mínima se graba únicamente el audio y el video en pantalla de la clase, evitando los desafíos técnicos y las dificultades que implica la grabación de otros elementos como la pizarra. Esto implica el uso de anotaciones en pantalla en tiempo real para reemplazar completamente las anotaciones en la pizarra.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. I Plan Propio Integral de Docencia. Universidad de Málaga. Universidad de Málaga. Vicerectorado de Personal Docente e Investigador, Proyectos de Innovación Educativa (PIE 17-018

Fracturas de las espinas tibiales en el niño y en el adulto

Presentamos una serie de 29 fracturas de las espinas tibiales en 27 pacientes, 11 niños y 16 adultos. Para su estudio hemos seguido la clasificación de Meyers y McKeever, siendo las fracturas tipo II las más frecuentes. Se registraron las lesiones asociadas de la rodilla, y la más frecuente fue la del ligamento lateral interno; encontramos 2 fracturas de Segond en niños. El tratamiento fue conservador en 7 casos y quirúrgico en los 22 restantes. Para evaluar el resultado final estudiamos el desplazamiento de la fractura, movilidad y laxitudes residuales de la rodilla y fuerza muscular. Usamos las escalas de Lysholm y de Tegner para la valoración funcional. Un estudio radiológico con 3 proyecciones se llevó a cabo en todos los pacientes. El tiempo medio de seguimiento fue de 9,7 años para el grupo de niños y de 8,2 para los adultos. Los resultados globales, de acuerdo con la escala de Lysholm, fueron excelentes, pero se registraron 11 laxitudes anteroposteriores y 4 laterales, aunque 11 de estos 15 pacientes permanecen asintomáticos. Muchas de estas laxitudes aparecen en fracturas anatómicamente reducidas. Sugerimos que debe realizarse estudio artroscópico en estos pacientes para detectar lesiones asociadas que empeoran el resultado.A series of 29 fractures of the tibial spine in 27 patients, 11 children and 16 adults. We followed Meyers and McKeever to classify the fractures, tipe II being the most frecuent. Associated injuries of the knee were registered, being medial collateal ligament the most frecuent. We found 2 Segond fractures in young patients. The treatment was conservative in 7 cases and surgical in the remaining 22. To evaluate the final result we studied the displacement of the fracture, mobility of the knee, anteroposterior and lateral inestabilities, muscle strength. We used the Lysholm and the Tegner rating systems to evaluate the functional result. A complete radiological evaluation (three views) was made in all the patients. The average follow-up times was 9.7 years for the children group, and 8.2 years for the adult group. The overall results according to the Lysholm rating scale were excellent, but 11 anteroposterior and 4 lateral laxities were registered, though 11 of the 15 cases remains asymptomatic. Many of theese laxities appeared in anatomically reduced fractures. We suggest that an arthroscopic study of the patients must be done to detect associated injuries that produces a worse final result

Reconstrucción ósea acetabular con cotilos no cementados y aporte de aloinjerto en la artroplastia total de cadera : estudio de 35 casos

Se han revisado 35 arlroplastias realizadas en 29 pacientes portadores de algún tipo de defecto óseo en el cotilo por fallo de prótesis total de cadera. En el presente estudio incluimos sólo las reconstrucciones acetabulares efectuadas con implantes no cementados. La edad media fue de 60 años (29/79), siendo 19 mujeres y 10 varones. Clasificamos los defectos óseos siguiendo a D'Antonio en segmentarios, cavitarios y mixtos, y para la reparación de dichos defectos utilizamos aloinjerto criopreservado en todos los casos excepto uno, tratado con injerto liofilizado. Valoramos los resultados desde un punto de vista clínico, subjetivo y objetivo (escala de Harris). Desde un punto de vista radiológico se analizaron las migraciones del implante acetabular, la existencia de zonas de radiolucencia, las osificaciones periarticulares y la incorporación o no del injerto óseo utilizado. Desde el punto de vista clínico obtuvimos un 60% de resultados excelentes y buenos, frente a un 40% de regulares y malos. Desde cualquier punto de vista el índice de aflojamiento del componente acetabular fue mucho mayor que el que puede obtenerse con cotilos no defectuososThirty five revision hip arthroplasties performed in 29 patients with acetabular bone defects were reviewed. In this series we only include the acetabular reconstruction performed using non cemented acetabular implants. The mean age of the patients was 60 years (29-79), 19 were female and 10 male. We classified the acetabular bone defects according to D'Antonio, in segmentary, cavitary and combined. To repair these defects, we used criopreserved allograft, except one case in which a liofilized allograft was used. The results were evaluated clinically (modified Harris hip score) and radiographically, looking for acetabular migrations, radiolucent lines, periarticular ossifications and bone graft incorporation. We obtained 60% of excellent and good results. There was 40% of poor and fair results. The acetabular loosening rate was higher than in revision hip arthroplasty without acetabular bone defects

Resultados a largo plazo de la patelectomía total

Desde el año 1981 hemos realizado 19 patelectomías totales, 9 por artrosis femoropatelar, 8 por dolor anterior de rodilla y 2 por fracturas conminutas. La edad media en el momento de la intervención era de 48 años (25-80); todos los pacientes con dolor anterior habían sido operados previamente. Con una evolución media de 7,8 años (3-14), hemos realizado una revisión clínica y radiológica. Según la escala de la Sociedad Internacional de Rodilla obtuvimos un 58% de resultados excelentes y buenos, resaltando que continúan con dolor todos los pacientes operados por artrosis y el 50% de los intervenidos por dolor anterior. La patelectomía total es una técnica cada vez menos utilizada, limitándose sus indicaciones a fracturas muy conminutas, artrosis femoropatelar en pacientes en los que estaría contraindicada la artroplastia total y en cuadros de dolor anterior de rodilla rebeldes a otros tratamientosNineteen total patelectomies were performed since 1981. The indication was femoropatellar osteoarthritis in nine cases, anterior knee pain syndrome in eight, and conminute fractures in two. The mean age of the group at the time of operation was 48 years (25-80). A1l the patients with anterior knee pain syndrome had been operated previously. With an average followup time of 7.8 years (3-14), vve reviewed our patients clinically and radiographically. We used the International Knee Society rating scale and found 58% of excellent and good results; 100% of the patients operated because of osteoarthritis and 50% of those operated because an anterior knee pain syndrome have pain at review. Total patellectomy is not a frequent technique. Today, the sole indications are very conminute fractures of the patella, femoropatellar osteoarthritis in those patients in which total knee arthroplasty is not indicated and in the anterior knee pain syndrome not improved with other treatments

Open sea OWC motions and mooring loads monitoring at BiMEP

This is the author accepted manuscript.Despite the large number of wave energy converter concepts proposed over the past three decades, only a few field measurement datasets are available in the public domain. The sparse nature of device performance and reliability data coupled with a general lack of design convergence means that technological and economic progress within the sector is fragmented. Fundamental to ensuring device efficiency and survivability is the acquisition of long-term, open sea, device and mooring system response data, combined with comprehensive numerical modelling. With mooring systems representing approximately 10% of marine renewable energy device CAPEX, the evolution of shared mooring systems and the use of novel materials with load reduction capabilities represent clear strategies to achieve more favourable project finances. This paper will report on design of the mooring load monitoring system as well as preliminary analysis of several load cases identified from field data recorded during the winter of the first deployment. Comparisons are made to numerical simulations of the device and mooring system subjected to representative environmental conditions. The measured mooring line tensions also provide operational design criteria (i.e. load capacity and durability requirements) for two elastomeric tethers which will replace the polyester ropes currently used in the seaward catenary lines.The research leading to this paper is part of the OPERA (Open Sea Operating Experience to Reduce Wave Energy Cost) project which is funded from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 654.444

Influência da carga pendente, do espaçamento e de fatores climáticos no desenvolvimento da ferrugem do cafeeiro.

A ferrugem é a principal doença da cultura do cafeeiro podendo causar significativas reduções na produtividade da lavoura. A ocorrência desta doença é influenciada por fatores climáticos, tais como chuvas contínuas e temperaturas médias. Fatores ligados à lavoura, como carga pendente e espaçamento também interferem na infecção do patógeno. Neste trabalho foi realizado o acompanhamento do índice de infecção de ferrugem em lavouras localizadas na Fazenda Experimental de Varginha, entre nos agrícolas de 1998/99 a 2006/2007. Para tanto, foram coletadas folhas de talhões sem controle de ferrugem, em lavouras com espaçamento adensado e largo, sendo que para os dois espaçamentos foram utilizadas lavouras com carga pendente alta e baixa. Observou-se que, independentemente do espaçamento, a infecção de ferrugem foi maior nas lavouras com carga pendente alta, na média de todos os anos avaliados. No atual ano agrícola 2006/07 o índice de infecção mostrou-se muito precoce e elevado em relação à média histórica apresentando valores médios de 60 a 70,0% já no mês de fevereiro

High-resolution melting assay for genotyping variants of the CYP2C19 enzyme and predicting voriconazole effectiveness

Voriconazole is a triazole antifungal agent recommended as primary treatment for invasive aspergillosis, as well as some other mold infections. However, it presents some pharmacokinetic singularities that lead to a great variability intra- and interindividually, nonlinear pharmacokinetics, and a narrow therapeutic range. Most experts have recommended tracing the levels of voriconazole in patients when receiving treatment. This azole is metabolized through the hepatic enzyme complex cytochrome P450 (CYPP450), with the isoenzyme CYP2C19 being principally involved. Allelic variations (polymorphisms) of the gene that encodes this enzyme are known to contribute to variability in voriconazole exposure. Three different allelic variants, CYP2C19*17, CYP2C19*2, and CYP2C19*3, could explain most of the phenotypes related to the voriconazole metabolism and some of its pharmacokinetic singularities. We designed a rapid molecular method based on high-resolution melting to characterize these polymorphisms in a total of 142 samples, avoiding sequencing. Three PCRs were designed with similar cycling conditions to run simultaneously. The results showed that our method represents a fast, accurate, and inexpensive means to study these variants related to voriconazole metabolism. In clinical practice, this could offer a useful tool to individually optimize therapy and reduce expenses in patients with fungal infections.National Institute of Health Carlos III (AES13PI13/01817Research Project MPY 1367/13). L.B.-M. has a contract supported by theMinisterio de Ciencia e Innovación, Instituto de Salud Carlos III, cofinanced by the EuropeanDevelopment Regional Fund (EDRF) “A Way to Achieve Europe” and the SpanishNetwork for the Research in Infectious Diseases (REIPI; RD12/0015/0015). B.M.-R. is astudent in the Master’s Program entitled “Microbiología Aplicada a la Salud Pública eInvestigación en Enfermedades Infecciosas,” Alcalá de Henares University, Madrid,Spain. A.C. and C.C. were supported by the Northern Portugal Regional OperationalProgram (NORTE 2020) under the Portugal 2020 Partnership Agreement through theEuropean Regional Development Fund (FEDER; NORTE-01-0145-FEDER-000013) and theFundação Para a Ciência e Tecnologia (FCT; IF/00735/2014 [A.C.] and SFRH/BPD/96176/2013 [C.C.])

The Plasmodium falciparum, Nima-related kinase Pfnek-4: a marker for asexual parasites committed to sexual differentiation

<b>Background</b> Malaria parasites undergo, in the vertebrate host, a developmental switch from asexual replication to sexual differentiation leading to the formation of gametocytes, the only form able to survive in the mosquito vector. Regulation of the onset of the sexual phase remains largely unknown and represents an important gap in the understanding of the parasite's complex biology. <b>Methods:</b> The expression and function of the Nima-related kinase Pfnek-4 during the early sexual development of the human malaria parasite Plasmodium falciparum were investigated, using three types of transgenic Plasmodium falciparum 3D7 lines: (i) episomally expressing a Pfnek-4-GFP fusion protein under the control of its cognate pfnek-4 promoter; (ii) episomally expressing negative or positive selectable markers, yeast cytosine deaminase-uridyl phosphoribosyl transferase, or human dihydrofolate reductase, under the control of the pfnek-4 promoter; and (iii) lacking a functional pfnek-4 gene. Parasite transfectants were analysed by fluorescence microscopy and flow cytometry. In vitro growth rate and gametocyte formation were determined by Giemsa-stained blood smears. <b>Results:</b> The Pfnek-4-GFP protein was found to be expressed in stage II to V gametocytes and, unexpectedly, in a subset of asexual-stage parasites undergoing schizogony. Culture conditions stimulating gametocyte formation resulted in significant increase of this schizont subpopulation. Moreover, sorted asexual parasites expressing the Pfnek-4-GFP protein displayed elevated gametocyte formation when returned to in vitro culture in presence of fresh red blood cells, when compared to GFP- parasites from the same initial population. Negative selection of asexual parasites expressing pfnek-4 showed a marginal reduction in growth rate, whereas positive selection caused a marked reduction in parasitaemia, but was not sufficient to completely abolish proliferation. Pfnek-4- clones are not affected in their asexual growth and produced normal numbers of stage V gametocytes. <b>Conclusions:</b> The results indicate that Pfnek-4 is not strictly gametocyte-specific, and is expressed in a small subset of asexual parasites displaying high rate conversion to sexual development. Pfnek-4 is not required for erythrocytic schizogony and gametocytogenesis. This is the first study to report the use of a molecular marker for the sorting of sexually-committed schizont stage P. falciparum parasites, which opens the way to molecular characterization of this pre-differentiated subpopulation