Thermophilic Sulfate Reduction in Hydrothermal Sediment of Lake Tanganyika, East Africa

In environments with temperatures above 60 degrees C, thermophilic prokaryotes are the only metabolically active life-forms. By using the (SO42-)-S-35 tracer technique, we studied the activity of sulfate-reducing microorganisms (SRM) in hot sediment from a hydrothermal vent site in the northern part of freshwater Lake Tanganyika (East Africa). Incubation of slurry samples at 8 to 90 degrees C demonstrated meso- and thermophilic sulfate reduction with optimum temperatures of 34 to 45 degrees C and 56 to 65 degrees C, respectively, and with an upper temperature limit of 80 degrees C. Sulfate reduction was stimulated at all temperatures by the addition of short-chain fatty acids and benzoate or complex substrates (yeast extract and peptone). A time course experiment showed that linear thermophilic sulfate consumption occurred after a lag phase (12 h) and indicated the presence of a large population of SRM in the hydrothermal sediment. Thermophilic sulfate reduction had a pH optimum of about 7 and was completely inhibited at pH 8.8 to 9.2. SRM could be enriched from hydrothermal chimney and sediment samples at 60 and 75 degrees C. In lactate-grown enrichments, sulfide production occurred at up to 70 and 75 degrees C, with optima at 63 and 71 degrees C, respectively. Several sporulating thermophilic enrichments were morphologically similar to Desulfotomaculum spp. Dissimilatory sulfate reduction in the studied hydrothermal area of Lake Tanganyika apparently has an upper temperature limit of 80 degrees C

Nutrient availability affects carbon turnover and microbial physiology differently in topsoil and subsoil under a temperate grassland

Increasing subsoil organic carbon inputs could potentially mitigate climate change by sequestering atmospheric CO2. Yet, microbial turnover and stabilization of labile carbon in subsoils are regulated by complex mechanisms including the availability of nitrogen (N), phosphorous (P), and sulfur (S). The present study mimicked labile organic carbon input using a versatile substrate (i.e. glucose) to address the interaction between carbon-induced mineralization, N-P-S availability, and microbial physiology in topsoil and subsoils from a temperate agricultural sandy loam soil. A factorial incubation study (42 days) showed that net losses of added carbon in topsoil were constant, whereas carbon losses in subsoils varied according to nutrient treatments. Glucose added to subsoil in combination with N was fully depleted, whereas glucose added alone or in combination with P and S was only partly depleted, and remarkably 59–92% of the added glucose was recovered after the incubation. This showed that N limitation largely controlled carbon turnover in the subsoil, which was also reflected by microbial processes where addition of glucose and N increased β-glucosidase activity, which was positively correlated to the maximum CO2 production rate during incubation. The importance of N limitation was substantiated by subsoil profiles of carbon source utilization, where microbial metabolic diversity was mainly related to the absence or presence of added N. Overall, the results documented that labile carbon turnover and microbial functions in a temperate agricultural subsoil was controlled to a large extent by N availability. Effects of glucoseinduced microbial activity on subsoil physical properties remained ambiguous due to apparent chemical effects of N (nitrate) on clay dispersibility

Horizontal activites. QLIF subproject 7: Horizontal activities

QLIF subproject 7 represents four horizontal activities common to the project, namely: • Environmental and sustainability audits • Cost-benefit analyses and socio-economic impact assessments • Dissemination and technology transfer • Training of graduate and postgraduate researchers Activities in the horizontal research have shown that organic crop production systems generally are more energy-efficient and have lower greenhouse gas emissions than the conventional production. In terms of dissemination the QLIF website has been central and the QLIF newsletter has attracted more than 1000 subscribers. Coupling of the website with the open access database Organic Eprints provides a prospective source of project information that can be accessed also by future stakeholders in organic and low-input systems. Training events arranged annually for students have contributed to proliferation of skills and knowledge gained in QLIF. Also, these events have served to mediate the attitude needed for research in organic and low-input farming

Trace Metal Availability Affects Greenhouse Gas Emissions and Microbial Functional Group Abundance in Freshwater Wetland Sediments

We investigated the effects of trace metal additions on microbial nitrogen (N) and carbon (C) cycling using freshwater wetland sediment microcosms amended with micromolar concentrations of copper (Cu), molybdenum (Mo), iron (Fe), and all combinations thereof. In addition to monitoring inorganic N transformations (NO3–, NO2–, N2O, NH4+) and carbon mineralization (CO2, CH4), we tracked changes in functional gene abundance associated with denitrification (nirS, nirK, nosZ), dissimilatory nitrate reduction to ammonium (DNRA; nrfA), and methanogenesis (mcrA). With regards to N cycling, greater availability of Cu led to more complete denitrification (i.e., less N2O accumulation) and a higher abundance of the nirK and nosZ genes, which encode for Cu-dependent reductases. In contrast, we found sparse biochemical evidence of DNRA activity and no consistent effect of the trace metal additions on nrfA gene abundance. With regards to C mineralization, CO2 production was unaffected, but the amendments stimulated net CH4 production and Mo additions led to increased mcrA gene abundance. These findings demonstrate that trace metal effects on sediment microbial physiology can impact community-level function. We observed direct and indirect effects on both N and C biogeochemistry that resulted in increased production of greenhouse gasses, which may have been mediated through the documented changes in microbial community composition and shifts in functional group abundance. Overall, this work supports a more nuanced consideration of metal effects on environmental microbial communities that recognizes the key role that metal limitation plays in microbial physiology

Fatty acid composition of sulfate-reducing bacteria isolated from deep-sea hydrothermal vent (13° N, East Pacific Rise)

Five strains of vibrio-shaped, mesophilic sulfate-reducing bacteria were isolated from the deep-sea hydrothermal vent site at 13° N on the East Pacific Rise. Phospholipid analyses demonstrated a high percentage of branched-chain fatty acids, including the known biomarker for Desulfovibrio, in all five strains. The cell-wall lipids showed a fatty acid composition markedly different from the phospholipids. While straight-chain fatty acids were predominant the biomarker fatty acid was absent. Based on the morphological characteristics and the fatty acid composition, we tentatively have assigned the isolates to the genus Desulfovibrio

Annual CO2 fluxes from a cultivated fen with perennial grasses during two initial years of rewetting

Rewetting combined with biomass crop cultivation (paludiculture) has been proposed as a method for reducing carbon dioxide (CO2) emissions from drained peatlands. This field experiment compared CO2 fluxes from drained (control) and rewetted experimental plots in a temperate fen under reed canary grass cultivation over two successive years. The annual weighted mean water table depth from soil surface (WTD) during the study period was 9, 3 and 1 cm in control, semi-flooded and flooded plots, respectively. There were no significant effects of WTD treatment on biomass yields. The choice of response model for CO2 fluxes influenced annual estimates of ecosystem respiration (ER) and gross primary production (GPP), but all models showed that ER and GPP decreased in response to rewetting. The resulting net ecosystem exchange (NEE) of CO2, derived by combining eight ER and eight GPP models, varied widely. For example, NEE (expressed as CO2-C) ranged from 935 to -208 g m 2 yr-1 for the flooded plots. One set of ER and GPP models was selected on the basis of statistical criteria and showed insignificant differences in NEE between the three water table treatments ( 537 to -341 g CO2-C m-2 yr 1). Treatment effects on CO2 emission factors, calculated as the sum of NEE and C export in harvested biomass (58–242 g CO2-C m-2 yr-1), were similarly insignificant. Thus, the results indicated that varying WTD within this narrow range could influence both ER and GPP without altering the net emissions of CO2

Water-table-driven greenhouse gas emission estimates guide peatland restoration at national scale

The substantial climate change mitigation potential of restoring peatlands through rewetting and intensifying agriculture to reduce greenhouse gas (GHG) emissions is largely recognized. The green deal in Denmark aims at restoring 100 000 ha of peatlands by 2030. This area corresponds to more than half of the Danish peatland, with an expected reduction in GHG emissions of almost half of the entire land use, land use change and forestry (LULUFC) emissions. Recent advances established the functional relationship between hydrological regimes, i.e., water table depth (WTD), and CO2 and CH4 emissions. This builds the basis for science-based tools to evaluate and prioritize peatland restoration projects. With this article, we lay the foundation of such a development by developing a high-resolution WTD map for Danish peatlands. Further, we define WTD response functions (CO2 and CH4) fitted to Danish flux data to derive a national GHG emission estimate for peat soils. We estimate the annual GHG emissions to be 2.6 Mt CO2-eq, which is around 15 % lower than previous estimates. Lastly, we investigate alternative restoration scenarios and identify substantial differences in the GHG reduction potential depending on the prioritization of fields in the rewetting strategy. If wet fields are prioritized, which is not unlikely in a context of a voluntary bottom-up approach, the GHG reduction potential is just 30 % for the first 10 000 ha with respect to a scenario that prioritizes drained fields. This underpins the importance of the proposed framework linking WTD and GHG emissions to guide a spatially differentiated peatland restoration. The choice of model type used to fit the CO2 WTD response function, the applied global warming potentials and uncertainties related to the WTD map are investigated by means of a scenario analysis, which suggests that the estimated GHG emissions and the reduction potential are associated with coefficients of variation of 13 % and 22 %, respectively.</p

Long‐term nitrogen loading alleviates phosphorus limitation in terrestrial ecosystems

Increased human‐derived nitrogen (N) deposition to terrestrial ecosystems has resulted in widespread phosphorus (P) limitation of net primary productivity. However, it remains unclear if and how N‐induced P limitation varies over time. Soil extracellular phosphatases catalyze the hydrolysis of P from soil organic matter, an important adaptive mechanism for ecosystems to cope with N‐induced P limitation. Here we show, using a meta‐analysis of 140 studies and 668 observations worldwide, that N stimulation of soil phosphatase activity diminishes over time. Whereas short‐term N loading (≤5 years) significantly increased soil phosphatase activity by 28%, long‐term N loading had no significant effect. Nitrogen loading did not affect soil available P and total P content in either short‐ or long‐term studies. Together, these results suggest that N‐induced P limitation in ecosystems is alleviated in the long‐term through the initial stimulation of soil phosphatase activity, thereby securing P supply to support plant growth. Our results suggest that increases in terrestrial carbon uptake due to ongoing anthropogenic N loading may be greater than previously thought.This study was funded by Aarhus University Centre for Circular Bioeconomy, Aarhus University Research Foundation AUFF Starting Grants (AUFF-E-2019-7-1), and Marie Skłodowska-Curie Individual Fellowship H2020-MSCA-IF-2018 (no. 839806). Ji Chen acknowledges funding support from the National Natural Science Foundation of China (41701292) and China Postdoctoral Science Foundation (2017M610647, 2018T111091) when constructing the databases. César Terrer was supported by a Lawrence Fellow award through Lawrence Livermore National Laboratory (LLNL). This work was performed under the auspices of the U.S. Department of Energy by LLNL under contract DEAC52-07NA27344 and was supported by the LLNL-LDRD Program under Project No. 20-ERD-055. Fernando T. Maestre was supported by the European Research Council (ERC Grant agreement 647038 [BIODESERT]) and Generalitat Valenciana (CIDEGENT/2018/041)