Antagonistic effect of probiotic strains against two pathogens: Salmonella Typhimurium and E. coli O157:H7

El objetivo de este estudio fue probar la eficiencia de las cepas probióticas Lactobacillus acidophilus NCFM (L. acidophilus), Lactobacillus rhamnosus HN001 (L. rhmanosus) y Bifidobacterium animalis BI07 (B. animalis) para antagonizar los patógenos resistentes a antibióticos Salmonella enteritidis var Thyphimurium (Salmonella Thyphimurium) y Escherichia coli O157:H7 (E. coli O157:H7). Las tres cepas probióticas mostraron poseer un efecto antagónico contra las cepas patógenas Salmonella Thyphimurium y E. coli O157:H7 resistentes a antibióticos. En la prueba de “Well Diffusion”, L. acidophilus y L. rhamnosus presentaron reducciones estadísticamente semejantes entre ellas (P >0.05) de 37-41 mm para E. coli O157:H7 y de 32 – 41 mm para Salmonella Thyphimurium, mientras que B. animalis mostró reducciones menores (P0.05).Consejo Nacional de Ciencia y TecnologíaGastronómica La Buena MesaJabil Circuit GuadalajaraKurago BiotekUniversidad de Guadalajar

Experimental Zika Virus Infection in the Pregnant Common Marmoset Induces Spontaneous Fetal Loss and Neurodevelopmental Abnormalities.

During its most recent outbreak across the Americas, Zika virus (ZIKV) was surprisingly shown to cause fetal loss and congenital malformations in acutely and chronically infected pregnant women. However, understanding the underlying pathogenesis of ZIKV congenital disease has been hampered by a lack of relevant in vivo experimental models. Here we present a candidate New World monkey model of ZIKV infection in pregnant marmosets that faithfully recapitulates human disease. ZIKV inoculation at the human-equivalent of early gestation caused an asymptomatic seroconversion, induction of type I/II interferon-associated genes and proinflammatory cytokines, and persistent viremia and viruria. Spontaneous pregnancy loss was observed 16-18 days post-infection, with extensive active placental viral replication and fetal neurocellular disorganization similar to that seen in humans. These findings underscore the key role of the placenta as a conduit for fetal infection, and demonstrate the utility of marmosets as a highly relevant model for studying congenital ZIKV disease and pregnancy loss

Spectroscopy and Orbital Analysis of Bright Bolides Observed over the Iberian Peninsula from 2010 to 2012

We present the analysis of the atmospheric trajectory and orbital data of four bright bolides observed over Spain, one of which is a potential meteorite dropping event. Their absolute magnitude ranges from -10 to -11. Two of these are of sporadic origin, although a Geminid and a kappa-Cygnid fireball are also considered. These events were recorded in the framework of the continuous fireball monitoring and spectroscopy campaigns developed by the SPanish Meteor Network (SPMN) between 2010 and 2012. The tensile strength of the parent meteoroids is estimated and the abundances of the main rock-forming elements in these particles are calculated from the emission spectrum obtained for three of these events. This analysis revealed a chondritic nature for these meteoroids.Comment: Manuscript in press in Monthly Notices of the Royal Astronomical Societ

Kinematics of the H2O masers at the centre of the PN K3-35

We have studied the kinematics traced by the water masers located at the centre of the planetary nebula (PN) K3-35, using data from previous Very Large Array (VLA) observations. An analysis of the spatial distribution and line-of-sight velocities of the maser spots allows us to identify typical patterns of a rotating and expanding ring in the position-velocity diagrams, according to our kinematical model. We find that the distribution of the masers is compatible with tracing a circular ring with a ~0.021 arcsec (~100 AU) radius, observed with an inclination angle with respect to the line of sight of 55 degrees. We derive expansion and rotation velocities of 1.4 and 3.1 km/s, respectively. The orientation of the ring projected on the plane of the sky, at PA 158 degrees, is almost orthogonal to the direction of the innermost region of the jet observed in K3-35, suggesting the presence of a disc or torus that may be related to the collimation of the outflow.Comment: 7 pages, 4 figures, 2 tables. Accepted by MNRA

Skin diseases in cetaceans. Scientific Committee document SC/60/DW8, International Whaling Commission, June 2008, Santiago, Chile

Micro-organisms that are known or suspected to cause skin diseases in cetaceans are briefly reviewed. Viruses belonging to four families i.e. Caliciviridae, Herpesviridae, Papillomaviridae and Poxviridae were detected by electron microscopy, histology and molecular techniques in vesicular skin lesions, black dots perceptible by the touch, warts and tattoos in several species of odontocetes and mysticetes. Herpesviruses, poxviruses and likely a cutaneous papillomavirus are cetacean specific. Among bacteria, Dermatophilus spp., Erysipelothrix rhusiopathiae, Mycobacterium marinum, Pseudomonas spp., Streptococcus iniae and Vibrio spp. were isolated from ulcerative dermatitis, pyogranulomatous dermatitis and panniculitis, diamond skin disease and slow-healing ulcers and abscesses. Aeremonas spp., Mycobacterium marinum, Pseudomonas spp. and Vibrio spp. are normally present in the marine environment while Erysipelothrix rhusiopathiae and Streptococcus iniae are fish pathogens that may also infect captive dolphins. Most seem to be opportunistic pathogens, exploiting some break-down in the host’s defenses to initiate an infection. Selection of antibiotic-resistant bacteria through the prophylactic use of antibiotics in aquaculture is suggested to be a growing problem in South America and may account for the emergence of unusual cutaneous conditions. At least four groups of fungi i.e. Candida albicans, Fusarium spp., Trichophyton spp. and Lacazia loboi cause skin diseases. Candidiasis occurs predominantly in captive odontocetes. The lesions are often localized around the body orifices and may become extensive, granulating and ulcerated. Fusariosis is characterized by firm, erythematous, cutaneous nodules. Trichophyton spp. was isolated from widespread superficial nodules in an Atlantic T. truncates kept in captivity in Japan. Lobomycosis or lacaziosis is distinguished by grayish, whitish to slightly pink, verrucuous lesions, often in pronounced relief that may ulcerate. While initially described only in Tursiops truncates and Sotalia guianensis from the Americas, lobomycosis seems to be expanding to other continents. The role of ballast water in transporting fungi worldwide should be investigated. Finally, ciliated protozoans, likely Kyaroikeus cetarius, caused invasive dermatitis in small cetaceans from the USA and Korea. The aquatic environment of cetaceans is naturally home to bacteria and fungi but cetacean skin has several mechanisms to impede invasion. Chemical contaminants may affect natural skin barriers and depress the immune system. Wounds and specific viral infection (poxvirus, herpesvirus) may provide routes of entry

Near-earth object 2012XJ112 as a source of bright bolides of achondritic nature

We analyse the likely link between the recently discovered near-Earth object 2012XJ112 and a bright fireball observed over the south of Spain on 2012 December 27. The bolide, with an absolute magnitude of -9 ± 1, was simultaneously imaged during the morning twilight from twometeor stations operated by the SPanish Meteor Network (SPMN). It was also observed by several casual witnesses. The emission spectrum produced during the ablation of the meteoroid in the atmosphere was also recorded. From its analysis, the chemical nature of this particle was inferred. Although our orbital association software identified several potential parent bodies for this meteoroid, the analysis of the evolution of the orbital elements performed with the MERCURY 6 symplectic integrator supports the idea that NEO 2012XJ112 is the source of this meteoroid. The implications of this potential association are discussed here. In particular, the meteoroid bulk chemistry is consistent with a basaltic achondrite, and this emphasizes the importance to deduce from future Earth approaches the reflectance spectrum and taxonomic nature of 2012XJ112. © 2014 The Author. Published by Oxford University Press on behalf of the Royal Astronomical Society.Ministerio de Ciencia y Educación AYA2009-13227, AYA2011-26522, AYA2009-06330-EJunta de Andalucía P09-FQM- 455

Methylation of subtelomeric chromatin modifies the expression of the lncRNA TERRA, disturbing telomere homeostasis

The long noncoding RNA (lncRNA) telomeric repeat-containing RNA (TERRA) has been associated with telomeric homeostasis, telomerase recruitment, and the process of chromosome healing; nevertheless, the impact of this association has not been investigated during the carcinogenic process. Determining whether changes in TERRA expression are a cause or a consequence of cell transformation is a complex task because studies are usually carried out using either cancerous cells or tumor samples. To determine the role of this lncRNA in cellular aging and chromosome healing, we evaluated telomeric integrity and TERRA expression during the establishment of a clone of untransformed myeloid cells. We found that reduced expression of TERRA disturbed the telomeric homeostasis of certain loci, but the expression of the lncRNA was affected only when the methylation of subtelomeric bivalent chromatin domains was compromised. We conclude that the disruption in TERRA homeostasis is a consequence of cellular transformation and that changes in its expression profile can lead to telomeric and genomic instability

Reshaping Curriculum to Enhance the Relevance of Literary Competence in Children's Education06

Twenty-first century learners face a multi-literacy landscape as they strive to acquire the cognitive skills needed for independent learning, apply linguistic skills to other knowledge base, and become computer literate. This article envisions a combination of skill sets and knowledge bases as the foundation of a literary competence-based curriculum. Such a curriculum would have the goal of enhancing young learners"critical thinking abilities; this would also help them take charge of the cognitive,linguistic, and sociocultural dimensions of written and spoken language in order to make learning transferable and applicable to the real world