Differential Expression Of Gap Junction mRNAs And Proteins In The Developing Murine Kidney And In Experimentally Induced Nephric Mesenchymes

The expression of three gap junction (GJ) proteins, alpha-1 (Cx43), beta-1 (Cx32), and beta-2 (Cx26), and their transcripts were examined during the ontogeny of the mouse and rat kidney. These proteins were expressed in two non-overlapping patterns. The alpha-1 GJ protein was first observed in mesenchymal cells in the 12-day mouse kidney. By day 14 and thereafter, the ai protein was detected in the transient S-shaped bodies, but not in the podocytes of the maturing glomeruli. After birth the antigen was retained in a small subset of secretory tubules.The beta-1 and beta-2 GJ proteins were similar in their developmental patterns. They were first detected in a small subset of secretory tubules in the subcortical zone of day 17 embryos. These tubules were identified by immunohistochemical markers to be proximal. At birth, practically all proximal tubules expressed the two antigens.This analysis of GJ proteins was consistent with the results of S1 nuclease protection assays showing that, while the alpha-1 mRNA appeared early during kidney development and declined around birth, the two beta mRNAs appeared later and became intensified during the last days of intrauterine development.In experimentally induced metanephric mesenchymes, a transient expression of the alpha-1 GJ protein was seen during the segregation of the tubular anlagen. beta-1 and beta-2 GJ proteins were not detected in such induced mesenchymes cultivated up to 7 days.These observations provide evidence for the cell-specific utilization of different GJ genes during different stages of kidney organogenesis. The alpha-1 gene is activated during the early segregation of the secretory tubule and might contribute to its compartmentalization, while the beta-1 and beta-2 gene products are not detected until advanced stages of development. The latter gene products might be correlated with the physiological activity of the proximal tubules in vivo, as they are not expressed in experimentally induced tubules detectable with markers for proximal tubules

The Contribution of the Light Quark Condensate to the Pion-Nucleon Sigma Term

There has been a discrepancy between values of the pion-nucleon sigma term extracted by two different methods for many years. Analysis of recent high precision pion-nucleon data has widened the gap between the two determinations. It is argued that the two extractions correspond to different quantities and that the difference between them can be understood and calculated.Comment: Modern Physics Letters A (in press

Scalar form-factor of the proton with light-cone QCD sum rules

In this article, we calculate the scalar form-factor of the proton in the framework of the light-cone QCD sum rules approach with the three valence quark light-cone distribution amplitudes up to twist-6, and observe the scalar form-factor $\sigma(t=-Q^2)$ at intermediate and large momentum transfers $Q^2> 2GeV^2$ has significant contributions from the end-point (or soft) terms. The numerical values for the $\sigma(t=-Q^2)$ are compatible with the calculations from the chiral quark model and lattice QCD at the region $Q^2>2GeV^2$.Comment: 18 pages, 7 figures, revised versio

Using multiple imputation and intervention-based scenarios to project the mobility of older adults

Background: Projections of the development of mobility limitations of older adults are needed for evidence-based policy making. The aim of this study was to generate projections of mobility limitations among older people in the United States, England, and Finland. Methods: We applied multiple imputation modelling with bootstrapping to generate projections of stair climbing and walking limitations until 2026. A physical activity intervention producing a beneficial effect on self-reported activities of daily living measures was identified in a comprehensive literature search and incorporated in the scenarios used in the projections. We utilised the harmonised longitudinal survey data from the Ageing Trajectories of Health – Longitudinal Opportunities and Synergies (ATHLOS) project (N = 24,982). Results: Based on the scenarios from 2012 to 2026, the prevalence of walking limitations will decrease from 9.4 to 6.4%. A physical activity intervention would decrease the prevalence of stair climbing limitations compared with no intervention from 28.9 to 18.9% between 2012 and 2026. Conclusions: A physical activity intervention implemented on older population seems to have a positive effect on maintaining mobility in the future. Our method provides an interesting option for generating projections by incorporating intervention-based scenarios

On the estimate of the sigma^(I = 1)_(KN)(0)-term value from the energy level shift of kaonic hydrogen in the ground state

Using the experimental data on the energy level shift of kaonic hydrogen in the ground state (the DEAR Collaboration, Phys. Rev. Lett. 94, 212302 (2005)) and the theoretical value of the energy level shift, calculated within the phenomenological quantum field theoretic approach to the description of strong low-energy anti-K N and anti-K NN interactions developed at Stefan Meyer Institut fuer subatomare Physik in Vienna, we estimate the value of the sigma^(I = 1)_(KN)(0)-term of low-energy anti-K N scattering. We get sigma^(I = 1)_(KN)(0) = (433 +/- 85) MeV. This testifies the absence of strange quarks in the proton structure.Comment: 7 pages, no figure

Sensitivity of barley varieties to weather in Finland

Global climate change is predicted to shift seasonal temperature and precipitation patterns. An increasing frequency of extreme weather events such as heat waves and prolonged droughts is predicted, but there are high levels of uncertainty about the nature of local changes. Crop adaptation will be important in reducing potential damage to agriculture. Crop diversity may enhance resilience to climate variability and changes that are difficult to predict. Therefore, there has to be sufficient diversity within the set of available cultivars in response to weather parameters critical for yield formation. To determine the scale of such ‘weather response diversity’ within barley (Hordeum vulgare L.), an important crop in northern conditions, the yield responses of a wide range of modern and historical varieties were analysed according to a well-defined set of critical agro-meteorological variables. The Finnish long-term dataset of MTT Official Variety Trials was used together with historical weather records of the Finnish Meteorological Institute. The foci of the analysis were firstly to describe the general response of barley to different weather conditions and secondly to reveal the diversity among varieties in the sensitivity to each weather variable. It was established that barley yields were frequently reduced by drought or excessive rain early in the season, by high temperatures at around heading, and by accelerated temperature sum accumulation rates during periods 2 weeks before heading and between heading and yellow ripeness. Low temperatures early in the season increased yields, but frost during the first 4 weeks after sowing had no effect. After canopy establishment, higher precipitation on average resulted in higher yields. In a cultivar-specific analysis, it was found that there were differences in responses to all but three of the studied climatic variables: waterlogging and drought early in the season and temperature sum accumulation rate before heading. The results suggest that low temperatures early in the season, delayed sowing, rain 3–7 weeks after sowing, a temperature change 3–4 weeks after sowing, a high temperature sum accumulation rate from heading to yellow ripeness and high temperatures (⩾25°C) at around heading could mostly be addressed by exploiting the traits found in the range of varieties included in the present study. However, new technology and novel genetic material are needed to enable crops to withstand periods of excessive rain or drought early in the season and to enhance performance under increased temperature sum accumulation rates prior to heading

Renormalization of relativistic baryon chiral perturbation theory and power counting

We discuss a renormalization scheme for relativistic baryon chiral perturbation theory which provides a simple and consistent power counting for renormalized diagrams. The method involves finite subtractions of dimensionally regularized diagrams beyond the standard $\bar{\rm MS}$ scheme of chiral perturbation theory to remove contributions violating the power counting. This is achieved by a suitable renormalization of the parameters of the most general effective Lagrangian. In addition to simplicity our method has the benefit that it can be easily applied to multiloop diagrams. As an application we discuss the mass and the scalar form factor of the nucleon and compare the results with the expressions of the infrared regularization of Becher and Leutwyler.Comment: 20 pages, RevTex, 1 figure, published version is shortene

Positive pion absorption on 3He using modern trinucleon wave functions

We study pion absorption on 3He employing trinucleon wave functions calculated from modern realistic NN interactions (Paris, CD Bonn). Even though the use of the new wave functions leads to a significant improvement over older calculations with regard to both cross section and polarization data, there are hints that polarization data with quasifree kinematics cannot be described by just two-nucleon absorption mechanisms.Comment: 14 pages, 6 figure