Application of accelerated heteronuclear single quantum coherence experiments to the rapid quantification of monosaccharides and disaccharides in dairy products

Monosaccharides and disaccharides are important dietary components, but if insufficiently metabolized by some population subgroups, they are also linked to disease patterns. Thus, the correct analytical identification, quantification, and labeling of these food components are crucial to inform and potentially protect consumers. Enzymatic assays and high-performance anion-exchange chromatography with pulsed amperometric detection are established methods for the quantification of monosaccharides and disaccharides that, however, require long measuring times (60–180 min). Accelerated methods for the identification and quantification of the nutritionally relevant monosaccharides and disaccharides d-glucose, d-galactose, d-fructose, sucrose, lactose, and maltose were therefore developed. To realize this goal, the NMR experiments HSQC (heteronuclear single quantum coherence) and acceleration by sharing adjacent polarization (ASAP)-HSQC were applied. Measurement times were reduced to 27 and 6 min, respectively, by optimizing the interscan delay and applying non-uniform sampling. The optimized methods were used to quantify d-glucose, d-galactose, d-fructose, sucrose, and lactose in various dairy products. Results of the HSQC and ASAP-HSQC methods are equivalent to the results of the reference methods in terms of both precision and accuracy, demonstrating that these methods can be used to correctly analyze nutritionally relevant monosaccharides and disaccharides in short times

Impact of earthworm activity on the chemical fertility of irrigated soil with urban effluents

The reuse of urban effluents to irrigate the soils of peri-urban grasslands in the vicinity of the town of Setif (northeastern Algeria) is an old and widespread practice. In this context, the present study was conducted to evaluate the effect of the irrigation with urban effluents on the biological and chemical behavior of soils. Effluents analysis showed significant organic and particulate pollution, the latter contributed to earthworm abundance and increased the richness of irrigated soils with nutrients. The analysis of turricules revealed the role of earthworms through the activity of bioturbation in the increase of the rate of organic matter as well as in the bioavailability of the nutrients of the irrigated soils. In space, permanent vegetation cover has played an important role as a biofilter. This was confirmed by the inter-site differences recorded through the measured variables particularly organic ones.Keywords: Natural grasslands, urban effluents, earthworm activity, turricles, organic matte

Covariants,joint invariants and the problem of equivalence in the invariant theory of Killing tensors defined in pseudo-Riemannian spaces of constant curvature

The invariant theory of Killing tensors (ITKT) is extended by introducing the new concepts of covariants and joint invariants of (product) vector spaces of Killing tensors defined in pseudo-Riemannian spaces of constant curvature. The covariants are employed to solve the problem of classification of the orthogonal coordinate webs generated by non-trivial Killing tensors of valence two defined in the Euclidean and Minkowski planes. Illustrative examples are provided.Comment: 60 pages. to appear in J. Math. Phy

Critical review of methods for risk ranking of food related hazards, based on risks for human health.

This study aimed to critically review methods for ranking risks related to food safety and dietary hazards on the basis of their anticipated human health impacts. A literature review was performed to identify and characterize methods for risk ranking from the fields of food, environmental science and socio-economic sciences. The review used a predefined search protocol, and covered the bibliographic databases Scopus, CAB Abstracts, Web of Sciences, and PubMed over the period 1993-2013. All references deemed relevant, on the basis of of predefined evaluation criteria, were included in the review, and the risk ranking method characterized. The methods were then clustered - based on their characteristics - into eleven method categories. These categories included: risk assessment, comparative risk assessment, risk ratio method, scoring method, cost of illness, health adjusted life years, multi-criteria decision analysis, risk matrix, flow charts/decision trees, stated preference techniques and expert synthesis. Method categories were described by their characteristics, weaknesses and strengths, data resources, and fields of applications. It was concluded there is no single best method for risk ranking. The method to be used should be selected on the basis of risk manager/assessor requirements, data availability, and the characteristics of the method. Recommendations for future use and application are provided

Assessment of organo-mineral fraction during co-composting of sewage sludge-lignocellulosic waste by XRD and FTIR analysis

This investigation focuses mainly on highlighting the contribution of the IR spectrum of the mineral matrix in co-composts after removing the organic phase by slow pyrolysis. The mineral matrix is still confusion subject in the interpretation of FTIR spectra of co-composts. The XRF and XRD analysis of the low pyrolysis substrates of sewage sludge and date palm waste during six months of co-composting, show that the mineral fraction is homogeneous in the samples and that the inorganic matrix consists mainly of silica and carbonates including a residual portion remaining after pyrolysis.The comparison of FTIR spectra of heated samples at 105°C and 650°C shows that the 3500-3280 cm-1 region characterises essentially the hydroxyl groups OH (ν(OH)) of water molecules. The observed bands in this region provide information on the co-compost hydrophilic nature but can only provide limited information about the humic substances composition. The band at 1035 cm-1, assigned commonly to organic material, which persists after the thermal treatment, should be assigned for the Si-O vibration of silica

Killing Vector Fields in Three Dimensions: A Method to Solve Massive Gravity Field Equations

Killing vector fields in three dimensions play important role in the construction of the related spacetime geometry. In this work we show that when a three dimensional geometry admits a Killing vector field then the Ricci tensor of the geometry is determined in terms of the Killing vector field and its scalars. In this way we can generate all products and covariant derivatives at any order of the ricci tensor. Using this property we give ways of solving the field equations of Topologically Massive Gravity (TMG) and New Massive Gravity (NMG) introduced recently. In particular when the scalars of the Killing vector field (timelike, spacelike and null cases) are constants then all three dimensional symmetric tensors of the geometry, the ricci and einstein tensors, their covariant derivatives at all orders, their products of all orders are completely determined by the Killing vector field and the metric. Hence the corresponding three dimensional metrics are strong candidates of solving all higher derivative gravitational field equations in three dimensions.Comment: 25 pages, some changes made and some references added, to be published in Classical and Quantum Gravit

Semiclassical States in Quantum Cosmology: Bianchi I Coherent States

We study coherent states for Bianchi type I cosmological models, as examples of semiclassical states for time-reparametrization invariant systems. This simple model allows us to study explicitly the relationship between exact semiclassical states in the kinematical Hilbert space and corresponding ones in the physical Hilbert space, which we construct here using the group averaging technique. We find that it is possible to construct good semiclassical physical states by such a procedure in this model; we also discuss the sense in which the original kinematical states may be a good approximation to the physical ones, and the situations in which this is the case. In addition, these models can be deparametrized in a natural way, and we study the effect of time evolution on an "intrinsic" coherent state in the reduced phase space, in order to estimate the time for this state to spread significantly.Comment: 21 pages, 1 figure; Version to be published in CQG; The discussion has been slightly reorganized, two references added, and some typos correcte

Cellular Communication through Light

Information transfer is a fundamental of life. A few studies have reported that cells use photons (from an endogenous source) as information carriers. This study finds that cells can have an influence on other cells even when separated with a glass barrier, thereby disabling molecule diffusion through the cell-containing medium. As there is still very little known about the potential of photons for intercellular communication this study is designed to test for non-molecule-based triggering of two fundamental properties of life: cell division and energy uptake. The study was performed with a cellular organism, the ciliate Paramecium caudatum. Mutual exposure of cell populations occurred under conditions of darkness and separation with cuvettes (vials) allowing photon but not molecule transfer. The cell populations were separated either with glass allowing photon transmission from 340 nm to longer waves, or quartz being transmittable from 150 nm, i.e. from UV-light to longer waves. Even through glass, the cells affected cell division and energy uptake in neighboring cell populations. Depending on the cuvette material and the number of cells involved, these effects were positive or negative. Also, while paired populations with lower growth rates grew uncorrelated, growth of the better growing populations was correlated. As there were significant differences when separating the populations with glass or quartz, it is suggested that the cell populations use two (or more) frequencies for cellular information transfer, which influences at least energy uptake, cell division rate and growth correlation. Altogether the study strongly supports a cellular communication system, which is different from a molecule-receptor-based system and hints that photon-triggering is a fine tuning principle in cell chemistry