NOUVELLE APPROCHE DE LA METHODE DES VOLUMES FINIS ‘MVF’ DEDIEE A LA RESOLUTION DES PROBLEMES ELECTROMAGNETIQUES 3D

Nous proposons dans le cadre de ce travail une nouvelle approche de la méthode des volumes finis dédié principalement à lamodélisation tridimensionnelle des phénomènes électromagnétiques au sein des dispositifs électrotechniques. Le maillageadopté est un maillage non-structuré qui requiert un mailleur indépendant, spécifique, parfaitement adapté à l’algorithmenumérique. Son rôle est de partitionner l’application étudiée en cellules élémentaires de forme aussi variée que des trianglesdans le cas bidimensionnel et tétraèdres voir prismatique dans le cas tridimensionnel. Cette approche est avantageuse comparéeaux approches présentées dans des travaux précédents par le faite quelle s’adapte parfaitement à la modélisationtridimensionnelle des problèmes électromagnétiques dans des dispositifs ayants des géométries complexe d’une part, d’autrepart, elle peut résoudre des problèmes électromagnétiques où les matériaux magnétiques sont utilisés dans la conception desdispositifs étudiés, contrairement à celles déjà présenté précédemment, où seulement on peut traiter les problèmes ayant desdispositifs conçut à partir des matériaux amagnétiques [1] voir diamagnétiques [2] comme par exemple les matériauxsupraconducteurs à haute température critique. L’ensemble des modèles mathématiques et numériques est implémenté dans uncode de calcul tridimensionnel sous environnement Matlab. La validité du travail présenté est atteinte par la comparaison desrésultats obtenus avec des résultats expérimentaux trouvés dans la littérature [3]

Recommendations for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samples.

Genotyping of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in monitoring viral evolution and transmission during the pandemic. The quality of the sequence data obtained from these genotyping efforts depends on several factors, including the quantity/integrity of the input material, the technology, and laboratory-specific implementation. The current lack of guidelines for SARS-CoV-2 genotyping leads to inclusion of error-containing genome sequences in genomic epidemiology studies. We aimed to establish clear and broadly applicable recommendations for reliable virus genotyping. We established and used a sequencing data analysis workflow that reliably identifies and removes technical artefacts; such artefacts can result in miscalls when using alternative pipelines to process clinical samples and synthetic viral genomes with an amplicon-based genotyping approach. We evaluated the impact of experimental factors, including viral load and sequencing depth, on correct sequence determination. We found that at least 1000 viral genomes are necessary to confidently detect variants in the SARS-CoV-2 genome at frequencies of ≥10%. The broad applicability of our recommendations was validated in over 200 clinical samples from six independent laboratories. The genotypes we determined for clinical isolates with sufficient quality cluster by sampling location and period. Our analysis also supports the rise in frequencies of 20A.EU1 and 20A.EU2, two recently reported European strains whose dissemination was facilitated by travel during the summer of 2020. We present much-needed recommendations for the reliable determination of SARS-CoV-2 genome sequences and demonstrate their broad applicability in a large cohort of clinical samples

TRPV1 in Brain Is Involved in Acetaminophen-Induced Antinociception

Background: Acetaminophen, the major active metabolite of acetanilide in man, has become one of the most popular overthe- counter analgesic and antipyretic agents, consumed by millions of people daily. However, its mechanism of action is still a matter of debate. We have previously shown that acetaminophen is further metabolized to N-(4-hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide (AM404) by fatty acid amide hydrolase (FAAH) in the rat and mouse brain and that this metabolite is a potent activator of transient receptor potential vanilloid 1 (TRPV1) in vitro. Pharmacological activation of TRPV1 in the midbrain periaqueductal gray elicits antinociception in rats. It is therefore possible that activation of TRPV1 in the brain contributes to the analgesic effect of acetaminophen. Methodology/Principal Findings: Here we show that the antinociceptive effect of acetaminophen at an oral dose lacking hypolocomotor activity is absent in FAAH and TRPV1 knockout mice in the formalin, tail immersion and von Frey tests. This dose of acetaminophen did not affect the global brain contents of prostaglandin E-2 (PGE(2)) and endocannabinoids. Intracerebroventricular injection of AM404 produced a TRPV1-mediated antinociceptive effect in the mouse formalin test. Pharmacological inhibition of TRPV1 in the brain by intracerebroventricular capsazepine injection abolished the antinociceptive effect of oral acetaminophen in the same test. Conclusions: This study shows that TRPV1 in brain is involved in the antinociceptive action of acetaminophen and provides a strategy for developing central nervous system active oral analgesics based on the coexpression of FAAH and TRPV1 in the brain

Pharmacologically Reversible, Loss of Function Mutations in the tm2 and tm4 Inner Pore Helices of Trek-1 k2p Channels

A better understanding of the gating of TREK two pore domain potassium (K2P) channels and their activation by compounds such as the negatively charged activator, flufenamic acid (FFA) is critical in the search for more potent and selective activators of these channels. Currents through wild-type and mutated human K2P channels expressed in tsA201 cells were measured using whole-cell patch-clamp recordings in the presence and absence of FFA. Mutation of the TM2.6 residue of TREK-1 to a phenylalanine (G171F) and a similar mutation of TM4.6 (A286F) substantially reduced current through TREK-1 channels. In complementary experiments, replacing the natural F residues at the equivalent position in TRESK channels, significantly enhanced current. Known, gain of function mutations of TREK-1 (G137I, Y284A) recovered current through these mutated channels. This reduction in current could be also be reversed pharmacologically, by FFA. However, an appropriate length MTS (MethaneThioSulfonate) cross-linking reagent (MTS14) restricted the activation of TREK-1_A286C channels by repeated application of FFA. This suggests that the cross-linker stabilises the channel in a conformation which blunts FFA activation. Pharmacologically reversible mutations of TREK channels will help to clarify the importance of these channels in pathophysiological conditions such as pain and depression

Numerical study of the influence of flux creep and of thermal effect on dynamic behaviour of magnetic levitation systems with a high- superconductor using control volume method

ID = 326International audienceThis paper displays some simulation results of dynamic responses of the high- superconductors (HTSC)-Permanent magnet (PM) levitation systems taking into account the influence of the flux creep phenomena and of the thermal effect. We focus on the establishment of a three-dimensional numerical code to solve the nonlinear and coupled equations. A new control volume method is proposed for the resolution of the partial derivative equations of the treaded physical phenomena. The influence is comprehensively displayed by comparing the predictions of dynamic responses of such systems in which the thermal effect in the superconductor is and is not taken into account. The electromagnetic and thermal coupling is ensured by an alternate algorithm. The thermal effect highlights the influence of the temperature on the value of the magnetic levitation force, levitation stabilization time and shows that the vibration center of levitated body had drifted downward