Portación de staphylococcus aureus y resistencia a la meticilina en elaboradores de alimentos de tres instituciones educativas de la ciudad de Gualeguaychú

Los elaboradores de alimentos que no mantienen una buena higiene pueden ser vehículos de Enfermedades Trasmitidas por Alimentos (ETA). En las últimas décadas se ha observado un aumento significativo de los portadores de Staphylococcus aureus resistente a la meticilina (SARM) representando un problema para la seguridad alimentaria y salud pública. El objetivo del presente trabajo fue detectar Staphylococcus aureus (SAU) y sensibilidad a meticilina, evaluando hábitos y conocimientos higiénico sanitarios de los elaboradores de alimentos de instituciones educativas de Gualeguaychú. Se analizaron 43 muestras de fosas nasales y manos de alumnos y docentes, realizando aislamiento de SAU, identificados mediante pruebas bioquímicas convencionales y sensibilidad a meticilina (difusión con disco de cefoxitina 30μg). Se evaluaron los hábitos y conocimientos aplicando un cuestionario de autoinforme. Los aspectos éticos fueron contemplados firmando un consentimiento. Resultados: El 47% (n=20) resulto positivo para SAU, 33% (n=14) lo portaban en manos y 35% (n=15) en narinas. La prevalencia de SAMR fue del 25% para narinas y 19% en manos. En el cuestionario se halló una diferencia significativa (ρ<0,05) para el momento de lavado de manos, manifiestando falta general de conocimiento. Conclusiones: si bien los valores de portación encontrados fueron similares a los de otros trabajos científicos, se encontró una frecuencia de resistencia a meticilina mayor, por lo que sería conveniente analizar qué ocurre en esta zona ya que no hay estudios reportados. Se deberían implementar capacitaciones, dado que las falencias detectadas indican circulación de SARM entre estos elaboradores de alimentos con el riesgo de ser diseminadores de dichas cepas.Fil: López, Tamara A.. Universidad Nacional de Entre Ríos.Fil: Grenóvero, Silvia. Universidad Nacional de Entre Ríos.Fil: Sosa, Natalia. Universidad Nacional de Entre Ríos.Fil: Taus Maria, R.. Universidad Nacional de Entre Ríos

Validación de firmas ológrafas

Existen, al día de hoy, una cantidad de alternativas en lo que hace a la validación de individuos. La mayoría de éstas presentan una serie de inconvenientes que las hace difícilmente masivas y/o suficientemente seguras. El presente trabajo profundiza acerca de los conceptos involucrados en dichas alternativas, y presenta la implementación práctica de una Solución Informática para validación de firmas manuscritas, denominada proyecto PI-1. El prototipo obtenido responde a una arquitectura original e introduce variantes que han surgido como producto de la investigación realizada en la combinación y optimización de ciertas técnicas. Se han concentrado los esfuerzos en obtener un procesamiento de tipo off-line de las firmas (denominado estático) a partir de imágenes capturadas desde un dispositivo celular móvil.Eje: Agentes y Sistemas inteligentesRed de Universidades con Carreras en Informática (RedUNCI

Validación de firmas ológrafas

Existen, al día de hoy, una cantidad de alternativas en lo que hace a la validación de individuos. La mayoría de éstas presentan una serie de inconvenientes que las hace difícilmente masivas y/o suficientemente seguras. El presente trabajo profundiza acerca de los conceptos involucrados en dichas alternativas, y presenta la implementación práctica de una Solución Informática para validación de firmas manuscritas, denominada proyecto PI-1. El prototipo obtenido responde a una arquitectura original e introduce variantes que han surgido como producto de la investigación realizada en la combinación y optimización de ciertas técnicas. Se han concentrado los esfuerzos en obtener un procesamiento de tipo off-line de las firmas (denominado estático) a partir de imágenes capturadas desde un dispositivo celular móvil.Eje: Agentes y Sistemas inteligentesRed de Universidades con Carreras en Informática (RedUNCI

Differential Micro RNA Expression in PBMC from Multiple Sclerosis Patients

Differences in gene expression patterns have been documented not only in Multiple Sclerosis patients versus healthy controls but also in the relapse of the disease. Recently a new gene expression modulator has been identified: the microRNA or miRNA. The aim of this work is to analyze the possible role of miRNAs in multiple sclerosis, focusing on the relapse stage. We have analyzed the expression patterns of 364 miRNAs in PBMC obtained from multiple sclerosis patients in relapse status, in remission status and healthy controls. The expression patterns of the miRNAs with significantly different expression were validated in an independent set of samples. In order to determine the effect of the miRNAs, the expression of some predicted target genes of these were studied by qPCR. Gene interaction networks were constructed in order to obtain a co-expression and multivariate view of the experimental data. The data analysis and later validation reveal that two miRNAs (hsa-miR-18b and hsa-miR-599) may be relevant at the time of relapse and that another miRNA (hsa-miR-96) may be involved in remission. The genes targeted by hsa-miR-96 are involved in immunological pathways as Interleukin signaling and in other pathways as wnt signaling. This work highlights the importance of miRNA expression in the molecular mechanisms implicated in the disease. Moreover, the proposed involvement of these small molecules in multiple sclerosis opens up a new therapeutic approach to explore and highlight some candidate biomarker targets in MS

Supplementary data for the article: Ristić, P.; Todorović, T. R.; Blagojević, V.; Klisurić, O. R.; Marjanović, I.; Holló, B. B.; Vulić, P.; Gulea, M.; Donnard, M.; Monge, M.; Rodríguez-Castillo, M.; López-de-Luzuriaga, J. M.; Filipović, N. R. 1D and 2D Silver-Based Coordination Polymers with Thiomorpholine-4-Carbonitrile and Aromatic Polyoxoacids as Coligands: Structure, Photocatalysis, Photoluminescence, and TD-DFT Study. Crystal Growth & Design 2020, 20 (7), 4461–4478. https://doi.org/10.1021/acs.cgd.0c00287

Supplementary material for: [https://pubs.acs.org/doi/10.1021/acs.cgd.0c00287#]Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/4200]Related to accepted version: [https://cherry.chem.bg.ac.rs/handle/123456789/4203

More, smaller bacteria in response to ocean's warming?

Heterotrophic bacteria play a major role in organic matter cycling in the ocean. Although the high abundances and relatively fast growth rates of coastal surface bacterioplankton make them suitable sentinels of global change, past analyses have largely overlooked this functional group. Here, time series analysis of a decade of monthly observations in temperate Atlantic coastal waters revealed strong seasonal patterns in the abundance, size and biomass of the ubiquitous flow-cytometric groups of low (LNA) and high nucleic acid (HNA) content bacteria. Over this relatively short period, we also found that bacterioplankton cells were significantly smaller, a trend that is consistent with the hypothesized temperature-driven decrease in body size. Although decadal cell shrinking was observed for both groups, it was only LNA cells that were strongly coherent, with ecological theories linking temperature, abundance and individual size on both the seasonal and interannual scale. We explain this finding because, relative to their HNA counterparts, marine LNA bacteria are less diverse, dominated by members of the SAR11 clade. Temperature manipulation experiments in 2012 confirmed a direct effect of warming on bacterial size. Concurrent with rising temperatures in spring, significant decadal trends of increasing standing stocks (3% per year) accompanied by decreasing mean cell size (-1% per year) suggest a major shift in community structure, with a larger contribution of LNA bacteria to total biomass. The increasing prevalence of these typically oligotrophic taxa may severely impact marine food webs and carbon fluxes by an overall decrease in the efficiency of the biological pump.Versión del editor5,064

Prognostic significance of FLT3-ITD length in AML patients treated with intensive regimens

FLT3-ITD mutations are detected in approximately 25% of newly diagnosed adult acute myeloid leukemia (AML) patients and confer an adverse prognosis. The FLT3-ITD allelic ratio has clear prognostic value. Nevertheless, there are numerous manuscripts with contradictory results regarding the prognostic relevance of the length and insertion site (IS) of the FLT3-ITD fragment. We aimed to assess the prognostic impact of these variables on the complete remission (CR) rates, overall survival (OS) and relapse-free survival (RFS) of AML patients with FLT3-ITDmutations. We studied the FLT3-ITD length of 362 adult AML patients included in the PETHEMA AML registry. We tried to validate the thresholds of ITD length previously published (i.e., 39 bp and 70 bp) in intensively treated AML patients (n = 161). We also analyzed the mutational profile of 118 FLT3-ITD AML patients with an NGS panel of 39 genes and correlated mutational status with the length and IS of ITD. The AUC of the ROC curve of the ITD length for OS prediction was 0.504, and no differences were found when applying any of the thresholds for OS, RFS or CR rate. Only four out of 106 patients had ITD IS in the TKD1 domain. Our results, alongside previous publications, confirm that FLT3-ITD length lacks prognostic value and clinical applicability. © 2021, The Author(s)

Unprecedented J-Aggregated Dyes in Pure Organic Solvents

We describe the design and synthesis of the first organic dyes enabling spontaneous formation of stable J-aggregates in common organic solvents without additives. The new dyes are O-BODIPYs with a B-spiranic 4,4-diacyloxyl substitution pattern. Key to the effectiveness of the J-aggregation process is the high conformational rigidity of the Bspiranic molecular design as well as the orthogonal disposition of the B-diacyloxyl substituent and the meso-aryl group with respect to the mean plane of the borodiazaindacene. Atomistic simulations, both in vacuum and in a solvent cage, support the dynamics of the J-aggregation process as well as its dependence on the alkylation pattern of the BODIPY chromophore. A detailed analysis of the photophysical and laser properties of the new dyes provides convincing evidence for the unambiguous assignment of these J-aggregates and their dependence on the environmental conditions.We gratefully acknowledge financial support by the Spanish Ministerio de Economia y Competitividad (projects MAT2014-51937-C3-1-P, MAT2014- 51937-C3-2-P, MAT2014-51937-C3-3-P, and MAT2015-68837-REDT). I. E. thanks the Gobierno Vasco (IT339-10) for a predoctoral contract. H. M. acknowledges the Spanish Ministerio de Economia y Competitividad for a Juan de la Cierva postdoctoral contract. The authors thank SGIker of UPV/EHU for technical and human support with the X-ray diffraction measurements and computational calculations, which were carried out in the “arina” informatic cluster. We also thank Diego Plaza Lozano for preliminary synthetic studies.Peer reviewe