Luxury Leaf LLC and Inner Sole

Kyle Oliveira and Tyler Johnson are the owners of two businesses, Luxury Leaf LLC and Inner Sole. Luxury Leaf manufactures hats, shirts, and other products out of hemp fiber, which has many qualities that makes it more sustainable than cotton. Inner Sole is a streetwear shop in Newport, Rhode Island that sells Luxury Leaf products alongside in-demand sneakers

Genetic recombination is targeted towards gene promoter regions in dogs

The identification of the H3K4 trimethylase, PRDM9, as the gene responsible for recombination hotspot localization has provided considerable insight into the mechanisms by which recombination is initiated in mammals. However, uniquely amongst mammals, canids appear to lack a functional version of PRDM9 and may therefore provide a model for understanding recombination that occurs in the absence of PRDM9, and thus how PRDM9 functions to shape the recombination landscape. We have constructed a fine-scale genetic map from patterns of linkage disequilibrium assessed using high-throughput sequence data from 51 free-ranging dogs, Canis lupus familiaris. While broad-scale properties of recombination appear similar to other mammalian species, our fine-scale estimates indicate that canine highly elevated recombination rates are observed in the vicinity of CpG rich regions including gene promoter regions, but show little association with H3K4 trimethylation marks identified in spermatocytes. By comparison to genomic data from the Andean fox, Lycalopex culpaeus, we show that biased gene conversion is a plausible mechanism by which the high CpG content of the dog genome could have occurred.Comment: Updated version, with significant revision

The Surprising Oceanography of the Gulf of Maine

The oceanography of the Gulf of Maine has changed in ways that have not been seen previously but that are likely to be more common in the future–changes like extreme rapid warming and declines in primary productivity. The changing oceanography has underpinned surprising losses in commercial stocks and endangered species. Because of the rapid rate of change, some have viewed the Gulf of Maine as a window into the ocean’s future, with the idea that lessons learned can be applied in places that have yet to experience similar rapid changes. We can examine the dynamics, origin, and implications of surprising oceanographic conditions–conditions that would have been considered unlikely based on recent prior experience. Based on a formal statistical definition of climate surprises, the frequency of oceanographic surprises in the Gulf of Maine is higher and has increased faster than what would be expected, even given underlying trends. Oceanographic surprises vary in character from one to the next and are often linked to larger scale shifting oceanography across the North Atlantic. The implications for ecological and human communities, industries, and conservation efforts imply a need for policies that consider adaptation to sudden events as well as long-term changes

Dissecting the difference in tree species richness between Africa and South America

peer reviewedSignificanceOur full-scale comparison of Africa and South America's lowland tropical tree floras shows that both Africa and South America's moist and dry tree floras are organized similarly: plant families that are rich in tree species on one continent are also rich in tree species on the other continent, and these patterns hold across moist and dry environments. Moreover, we confirm that there is an important difference in tree species richness between the two continents, which is linked to a few families that are exceptionally diverse in South American moist forests, although dry formations also contribute to this difference. Plant families only present on one of the two continents do not contribute substantially to differences in tree species richness

The strengths and weaknesses of species distribution models in biome delimitation

This is the final published version, also available from Frontiers via the DOI in this record.Aim: The aim was to test whether species distribution models (SDMs) can reproduce major macroecological patterns in a species-rich, tropical region and provide recommendations for using SDMs in areas with sparse biotic inventory data. Location: North-east Brazil, including Minas Gerais. Time period: Present. Major taxa studied: Flowering plants. Methods: Species composition estimates derived from stacked SDMs (s-SDMs) were compared with data from 1,506 inventories of 933 woody plant species from north-east Brazil. Both datasets were used in hierarchical clustering analyses to delimit floristic units that correspond to biomes. The ability of s-SDMs to predict the identity, functional composition and floristic composition of biomes was compared across geographical and environmental space. Results: The s-SDMs and inventory data both resolved four major biomes that largely corresponded in terms of their distribution, floristics and function. The s-SDMs proved excellent at identifying broad-scale biomes and their function, but misassigned many individual sites in complex savanna–forest mosaics. Main conclusions: Our results show that s-SDMs have a unique role to play in describing macroecological patterns in areas lacking inventory data and for poorly known taxa. s-SDMs accurately predict floristic and functional macroecological patterns but struggle in areas where non-climatic factors, such as fire or soil, play key roles in governing distributions.Natural Environment Research Council (NERC)Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorConselho Nacional de Desenvolvimento Científico e TecnológicoRoyal Societ

The architecture of amyloid-like peptide fibrils revealed by X-ray scattering, diffraction and electron microscopy

Structural analysis of protein fibrillation is inherently challenging. Given the crucial role of fibrils in amyloid diseases, method advancement is urgently needed. A hybrid modelling approach is presented enabling detailed analysis of a highly ordered and hierarchically organized fibril of the GNNQQNY peptide fragment of a yeast prion protein. Data from small-angle X-ray solution scattering, fibre diffraction and electron microscopy are combined with existing high-resolution X-ray crystallographic structures to investigate the fibrillation process and the hierarchical fibril structure of the peptide fragment. The elongation of these fibrils proceeds without the accumulation of any detectable amount of intermediate oligomeric species, as is otherwise reported for, for example, glucagon, insulin and [alpha]-synuclein. Ribbons constituted of linearly arranged protofilaments are formed. An additional hierarchical layer is generated via the pairing of ribbons during fibril maturation. Based on the complementary data, a quasi-atomic resolution model of the protofilament peptide arrangement is suggested. The peptide structure appears in a [beta]-sheet arrangement reminiscent of the [beta]-zipper structures evident from high-resolution crystal structures, with specific differences in the relative peptide orientation. The complexity of protein fibrillation and structure emphasizes the need to use multiple complementary methods