Deteksi Antibakteri pada Ekstrak Daun Murbei (morus albal.) Dari Beberapa Lokasi Pengambilan Sampel Tanaman di Sulawesi Selatan

Abstract: Abstract: Antibacterial Detection on Murbei leaves (Morus alba L.) from Various Sample Location in South Sulawesi. Mulberry (Morus alba L.) is one of the morus genus species known to have secondary metabolite compound that can be used as an antioxidant, antibacterial, antiviral, antidiabetic, antihypertensive, hypolipidemic, fever, flu, cough, antiemetic and gastrointestinal disorders. This research aimed to determine antibacterial activity of mulberry leaves from Soppeng, Gowa, and Enrekang against the bacteria Escherichia coli. Mulberry leaves were extracted using ethanol 70 % and ethyl acetate. Antibacterial activity test method was done by using agar diffusion method and TLC bioautography. The results showed that mulberry leaf extract from Soppeng, Gowa and Enrekang had antibacterial activity potential to Escherichia coli bacteria and the one which had good antibacterial activity was ethanol extract from Soppeng on concentration of 10% with diameter 9,89 mm on its inhibition zone. The test result of TLC bioautography test on acetate ethyl extracts showed that there was antibacterial activity seen from the stain spots, Soppeng 5 spots, Gowa 6 spots, and Enrekang 6 spots while the ethanol extract did not show any antibacterial activity against Escherichia coli

UJI EFEKTIVITAS KOMBINASI SARI BUAH MERAH (Pandanus conoideus Lam.) DAN PERASAN DAUN KUMIS KUCING (Orthosiphon aristatus Mig.) SEBAGAI ANTIINFLAMASI PADA MENCIT (Mus musculus) SECARA TOPIKAL

Inflammation is a protective response on the body from injury of cell. Edema is one of inflammation sign. It was reported that Pandanus conoideus Lam. and Orthosiphon aristatus Mig. empirically used as an antiinflammation. The aim of this research was determining the combination effect of Pandanus conoideus Lam. and Orthosiphon aristatus Mig. based on formation edema volume on sole of foot male mice againts carrageenan, inflammation that occurs is measured using the pletysmometer. In the research, we used sodium CMC as a negative control and sodium diclofenac as a positive control administered topically. While combination of Pandanus conoideus Lam. and Orthosiphon aristatus Mig. with a ratio of 2:1. The result showed decrease edema with percent of inhibition 76,92% Respectively, as a conclussion the result indicate Natrium diklofenak didn't showed significant effect compare with the testing data

ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT DARI LIUR BASA (LIMBAH SAYUR BAYAM DAN SAWI)

Lactic acid bacteria (BAL) is a gram-positive bacterium in the form of stems or round, not forming spores, anaerobic facultative fermentation and has the ability to ferment glucose into lactic acid. The purpose of this study was to isolate and identify lactic acid bacteria from spinach and mustard vegetable waste which can be used as food preservative. The isolation result was obtained by 10 isolates suspected as lactic acid bacteria. The isolates obtained were identified macroscopically, fermentation test, gram staining, spore staining and catalase test. From the macroscopic identification of samples of spinach and mustard, the isolates were white milk, rounded circular edges, kensitri surface and convex elevation. The fermentation test indicates that are heterofermentative and. All isolates obtained in the form of gram-positive bacteria had no negative spores and catalases and its alleged are Lactobasillus

PKM Pada Kelompok Masyarakat Desa Galesong Baru Kecamatan Galesong Kabupaten Takalar Tentang Pemanfaatan Obat Tradisional

 One area in Takalar district that still uses traditional medicine as an alternative treatment is Galesong Baru village. The use of traditional medicine in this area is based on traditonally principles, or ancestral recipes, customs, beliefs, or local customs. This service activity aims to provide knowledge and skills to the Galesong Baru community about the use of traditional medicine. The Activities are carried out online and offline using lecture methods, discussions, video screenings of traditional medicinal processing and distribution of books on traditional medicinal plants. The counseling materials provided included the definition of traditional medicine, general instructions for the use of traditional medicine and the provision of examples of traditional medicinal ingredients such as Zingiber cassumunar, Kaempferia galanga L, Zingiber officinale Rosc, Zingiber officinale varietas rubrum, Andrographis paniculata and Piper betle. The results of community service activities have provided increased knowledge and skills from the community on how to utilisation of traditional plants.Keywords : Traditional medicine, Dedicated to Community, Galesong BaruSalah satu wilayah di kabupaten Takalar yang masih memanfaatkan obat tradisional sebagai alternative pengobatan adalah desa Galesong Baru. Penggunaan obat tradisional di wilayah ini berdasarkan prinsip turun temurun, atau resep nenek moyang , adat-istiadat, kepercayaan, atau kebiasaan setempat. Kegiatan pengabdian ini bertujuan untuk memberikan pengetahuan dan keterampilan kepada masyarakat Galesong Baru tentang penggunaan obat tradisional. Kegiatan dilakukan secara daring maupun luring dengan menggunakan metode ceramah, diskusi, pemutaran video pengolahan obat tradisional serta pembagian buku tanaman obat tradisional. Materi penyuluhan yang diberikan meliputi pengertian obat tradisional, petunjuk umum penggunaan obat tradisional dan pemberian contoh ramuan obat tradisional seperti bangle, kencur, jahe merah, jahe, sambiloto dan sirih. Hasil Kegiatan pengabdian telah memberikan peningkatan pengetahuan dan keterampilan dari masyarakat tentang cara pemamfaatan tanaman tradisional.Kata Kunci : Pengobatan tradisional, Pengabdian kepada masyaraka

IDENTIFIKASI BIOKIMIA DAN AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI ASAM LAKTAT DARI LIMBAH SAYUR SAWI (Brassica juncea L.)

Lactic Acid Bacteria (LAB) is a group of bacteria that produce lactic acid as the main product in carbohydrate  fermentation. LAB has a major role to fight against pathogenic bacteria through peptide compounds. LAB can be found in vegetables that contain carbohydrates. The research objective was to determine biochemically and determine the antibacterial activity of LAB isolates from Brassica juncea L vegetable waste. Biochemical testing methods include TSIA, Indol, MR_VP, motility, temperature resistance and salt resistance tests. While testing the activity using the disc diffution method is used by the media Mueller Hinton Agar against Basillus subtilis,  Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Salmonella thypimurium, and Propionibacterium acnes.  Preparation of the test sample was carried out by inoculating LAB  isolate stock for 1x24 hours at 37 ° C. Biochemical identification results showed that LAB isolates were able to ferment glucose, negative in indole and VP testing, positive in MR testing was characterized by obtaining acidic pH, non-motile nature, living at 37 ° C, and resistant to several salt concentrations (5-10%). The results of testing antibacterial activity based on univariate analysis showed differences in the activity of each of the test bacteria obtained p = 0,730 (p>0,05). LSD analysis showed no significant differences between all isolates. Conclusion: isolates obtained included LAB  group from the family Lactobacillaceae, genus Lactobacillus sp and the antibacterial activity was broad spectru

UJI AKTIVITAS ANTIOKSIDAN DARI BEBERAPA EKSTRAK KULIT BATANG JAMBLANG (Syzygium cumini) MENGGUNAKAN METODE PEREDAMAN RADIKAL 2,2-DIPHENYL-1-PICRYLHYDRAZYL (DPPH)

Jamblang (Syzygium cumini) merupakan salah satu buah lokal Indonesia. Semua bagian tanaman ini dapat digunakan untuk pengobatan salah satunya sebagai antioksidan. Tujuan dari penelitian ini adalah untuk menentukan potensi antioksidan dari beberapa ekstrak kulit batang jamblang dengan menggunakan kuarcetin sebagai pembanding. Kulit batang jamblang diekstraksi secara refluks dengan menggunakan pelarut etanol, etil asetat, dan n-heksan kemudian di uji potensi antioksidannya. Hasil penelitian diperoleh nilai IC50 ekstrak etanol kulit batang jamblang sebesar 164,3 ppm, ekstrak etil asetat kulit batang jamblang sebesar 237,7 ppm, ekstrak n-heksan kulit batang jamblang sebesar 5235,6 ppm. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol kulit batang jamblang memberikan aktivitas antioksidan yang lebih kuat jika dibandingkan dengan ekstrak lainnya. Namun hasil tersebut masih lebih kecil jika dibandingkan dengan kuarcetin dengan nilai IC50 4,57 ppm

EDUKASI MASYARAKAT TERHADAP TANAMAN OBAT TRADISIONAL UNTUK MENINGKATKAN IMUNITAS DI DESA PAKKATTO

The Covid-19 pandemic is still engulfing the world, therefore every individual needs to keep his body's immune system strong so that it is not easy to contract viral diseases. A strong immune system has a very important role in fighting viruses. One way that is taken in an effort to maintain health and maintain and increase immunity is to take traditional medicines to avoid disease attacks, including Covid-19. The purpose of this activity is to provide education on the importance of traditional medicinal plants that are around us to increase the body's immunity. With this educational activity, it will be achieved for people who understand the benefits of traditional medicinal plants as home medicinal plants which are part of herbs that can be used to prevent the corona virus by increasing human immunity