The dynamics of hydro-ecological indicators of rivers in the area of placement mining enterprises of region KMA

The results of the comparative analysis of hydro-chemical status of surface water bodies exposing of mining, on the example of the small rivers of the Belgorod region according to the results of the research carried out in 2007-2014 are presented. It is shown that the production of iron mine (Yakovlevsky mine) is influenced on the hydroecological state of the Vorskla river for over 60 k

Геморрагический инсульт: нейрофизиологические предикторы острого периода

Background. Stroke is the most important medical and social problem due to its high proportion of morbidity, disability and mortality among patients of working age.Aims. The aim of the study is to predict the course of the acute period of hemorrhagic parenchymal stroke (supratentorial hemispheric hematomas) based on neuroimaging data (localization, lateralization, hematoma volume) and neurophysiological data.Materials and methods. 86 patients suffering from hemorrhagic stroke of supratentorial localization were examined. The level of consciousness, cognitive functions, and neuroimaging data were evaluated (EEG, heart rate variability,  event-related auditory potential). The condition of patients on admission and over time was assessed on the basis of the Glasgow Coma Scale (GCS), the expanded Glasgow Coma Scale and the NIHSS.Results. Based on the results of cluster analysis and expert assessments, two groups of patients were identified: with a relatively favorable and unfavorable course of the acute period of hemorrhagic stroke. Differences in neurophysiological parameters in the groups were established: an increase in the power of theta  oscillations and a decrease in the frequency of theta oscillations of the electroencephalogram, a decrease in the amplitude of the N2P2-component of the cognitive evoked P300 potential, an increase in heart rate in an unfavorable course. An artificial neural network has been created to predict the course of the acute period of hemorrhagic stroke upon admission.Conclusion. Machine learning methods allow creating algorithms for predicting the level of consciousness of patients, the acute period of development of intracerebral hematomas of supratentorial localization, the possible development of disease outcomes in patients with non-traumatic intracerebral hematomas based on neurophysiological parameters, as well as the volume of hematoma. A correlate of the unfavorable dynamics turned out to be a reduced bioelectrogenesis in the associative zones of the cortex during stimulus recognition  and decision-making, as well as the unfavorable dynamics of the level of consciousness corresponded to a decrease in the amplitude and greater latency of P2N2-peaks, reflecting insufficient activation of the cortical structures during stimulus recognition

Pharmacogenetic variability and the probability of site of action target attainment during tuberculosis meningitis treatment: a physiologically based pharmacokinetic modeling and simulations study

Objective and methodsOur objective was to investigate the role of patient pharmacogenetic variability in determining site of action target attainment during tuberculous meningitis (TBM) treatment. Rifampin and isoniazid PBPK model that included SLCO1B1 and NAT2 effects on exposures respectively were obtained from literature, modified, and validated using available cerebrospinal-fluid (CSF) concentrations. Population simulations of isoniazid and rifampin concentrations in brain interstitial fluid and probability of target attainment according to genotypes and M. tuberculosis MIC levels, under standard and intensified dosing, were conducted.ResultsThe rifampin and isoniazid model predicted steady-state drug concentration within brain interstitial fluid matched with the observed CSF concentrations. At MIC level of 0.25 mg/L, 57% and 23% of the patients with wild type and heterozygous SLCO1B1 genotype respectively attained the target in CNS with rifampin standard dosing, improving to 98% and 91% respectively with 35 mg/kg dosing. At MIC level of 0.25 mg/L, 33% of fast acetylators attained the target in CNS with isoniazid standard dosing, improving to 90% with 7.5 mg/kg dosing.ConclusionIn this study, the combined effects of pharmacogenetic and M. tuberculosis MIC variability were potent determinants of target attainment in CNS. The potential for genotype-guided dosing during TBM treatment should be further explored in prospective clinical studies.Pharmacolog

Mycobacterium tuberculosis Beijing Genotype and Risk for Treatment Failure and Relapse, Vietnam

Among 2,901 new smear-positive tuberculosis cases in Ho Chi Minh City, Vietnam, 40 cases of treatment failure and 39 relapsing cases were diagnosed. All initial and follow-up Mycobacterium tuberculosis isolates of these case-patients had (nearly) identical restriction fragment length polymorphism patterns, and the Beijing genotype was a significant risk factor for treatment failure and relapse (odds ratio 2.8, 95% confidence interval 1.5 to 5.2)

Mycobacterium tuberculosis Beijing genotype strains associated with febrile response to treatment.

DNA fingerprinting has demonstrated predominance of the Beijing genotype among Mycobacterium tuberculosis strains isolated in Southeast Asia. We prospectively examined the occurrence of Beijing genotype strains in tuberculosis patients in Indonesia. Early in treatment, patients infected with Beijing genotype strains more often had fever unrelated to disease severity, toxicity, or drug resistance, indicating that Beijing genotype strains may have specific pathogenic properties

Изотопный состав зимних атмосферных осадков и снежного покрова в предгорьях Алтая

Over the past three decades, several general circulation models of the atmosphere and ocean (atmospheric and oceanic general circulation models  – GCMs) have been improved by modeling the hydrological cycle with the use of isotopologues (isotopes of water) HDO and H2 18O. Input parameters for the GCM models taking into account changes in the isotope composition of atmospheric precipitation were, above all, the results obtained by the network GNIP – Global Network of Isotopes in Precipitation. At different times, on the vast territory of Russia there were only about 40 simultaneously functioning stations where the sampling of atmospheric precipitation was performed. In this study we present the results of the isotope composition of samples taken on the foothills of the Altai during two winter seasons of 2014/15 and 2015/16. Values of the isotope composition of precipitation changed in a wide range and their maximum fluctuations were 25, 202 and 18‰ for δ18О, dexc and δD, respectively. The weighted-mean values of δ18О and δD of the precipitation analyzed for the above two seasons were close to each other (−21.1 and −158.1‰ for the first season and −21.1 and −161.9‰ for the second one), while dexc values differed significantly. The comparison of the results of isotope analysis of the snow cover integral samples with the corresponding in the time interval the weighted-mean values of precipitation showed high consistency. However, despite the similarity of values of δ18О and δD, calculated for precipitation and snow cover, and the results, interpolated in IsoMAP (from data of the GNIP stations for 1960–2010), the dexc values were close to mean annual values of IsoMAP for only the second winter season. According to the trajectory analysis (the HYSPLIT model), the revealed differences between both, the seasons, and the long-term average values of IsoMAP, were associated with a change of main regions where the air masses carrying precipitation were formed, namely, the North Atlantic (the winter season of 2014/15) and the inland areas with open ice-free water bodies (the season of 2015/16). Thus, with the correct interpretation of the results, the data on the snow cover isotope composition on the Altai foothills can be used as an alternative data sources instead of the GNIP data.Приводятся результаты изотопного анализа состава атмосферных осадков и снежного покрова предгорий Алтая. Показано, что средневзвешенные значения осадков двух зимних сезонов (2014/15 и 2015/16 гг.) для δ18О составили −21,1‰, а для δD −158,1 и −161,9‰ соответственно и хорошо согласуются с изотопным составом интегральных проб снежного покрова. Проявившиеся отличия в dexc, очевидно, связаны со сменой основных регионов формирования воздушных масс, приносящих атмосферные осадки: сменой открытых ото льда акваторий Северной Атлантики на внутриконтинентальные водоёмы. При корректной интерпретации результатов данные изотопного состава атмосферных осадков и снежного покрова в предгорьях Алтая могут быть использованы в ряде моделей МОЦ

Mycobacterium tuberculosis Cluster with Developing Drug Resistance, New York, New York, USA, 2003–2009

In 2004, identification of patients infected with the same Mycobacterium tuberculosis strain in New York, New York, USA, resulted in an outbreak investigation. The investigation involved data collection and analysis, establishing links between patients, and forming transmission hypotheses. Fifty-four geographically clustered cases were identified during 2003–2009. Initially, the M. tuberculosis strain was drug susceptible. However, in 2006, isoniazid resistance emerged, resulting in isoniazid-resistant M. tuberculosis among 17 (31%) patients. Compared with patients with drug-susceptible M. tuberculosis, a greater proportion of patients with isoniazid-resistant M. tuberculosis were US born and had a history of illegal drug use. No patients named one another as contacts. We used patient photographs to identify links between patients. Three links were associated with drug use among patients infected with isoniazid-resistant M. tuberculosis. The photographic method would have been more successful if used earlier in the investigation. Name-based contact investigation might not identify all contacts, particularly when illegal drug use is involved

Vaccine-induced Immunity Circumvented by Typical Mycobacterium tuberculosis Beijing Strains

The frequency of typical and atypical Beijing strains of Mycobacterium tuberculosis was determined in the Netherlands; Vietnam; and Hong Kong Special Administrative Region, People’s Republic of China. The strains’ associations with drug resistance, M. bovis BCG vaccination, and patient characteristics were assessed. BCG vaccination may have positively selected the prevalent typical Beijing strains

Investigation on Mycobacterium tuberculosis Diversity in China and the Origin of the Beijing Clade

Contains fulltext : 124314.pdf (publisher's version ) (Open Access

IS6110-Restriction Fragment Length Polymorphism and Spoligotyping Analysis of Mycobacterium tuberculosis Clinical Isolates for Investigating Epidemiologic Distribution in Korea

The Beijing family of Mycobacterium tuberculosis has been emerging in the world. However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates of M. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M. tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview of the distribution of genotypes of M. tuberculosis in Korea. These findings indicate that we have to pay more attention to control of M. tuberculosis strains associated with the Beijing family