Millennial-scale perspective on biodiversity conservation of the forest-steppe ecotone in Europe

Paleoecological studies document that grasslands with a high conservation value in the European forest-steppe ecotone are often anthropogenically derived, requiring management to avoid woodland recovery. Nevertheless, naturally open areas often persisted within this ecotone, explaining the high richness of these grassland

Changes in species composition and diversity of a montane beetle community over the last millennium in the High Tatras, Slovakia : Implications for forest conservation and management

Montane biomes are niche environments high in biodiversity with a variety of habitats. Often isolated, these non-continuous remnant ecosystems inhabit narrow ecological zones putting them under threat from changing climatic conditions and anthropogenic pressure. Twelve sediment cores were retrieved from a peat bog in Tatra National Park, Slovakia, and correlated to each other by wiggle-matching geochemical signals derived from micro-XRF scanning, to make a reconstruction of past conditions. A fossil beetle (Coleoptera) record, covering the last 1000 years at 50- to 100-year resolution, gives a new insight into changing flora and fauna in this region. Our findings reveal a diverse beetle community with varied ecological groups inhabiting a range of forest, meadow and synanthropic habitats. Changes in the beetle community were related to changes in the landscape, driven by anthropogenic activities. The first clear evidence for human activity in the area occurs c. 1250 CE and coincides with the arrival of beetle species living on the dung of domesticated animals (e.g. Aphodius spp.). From 1500 CE, human (re)settlement, and activities such as pasturing and charcoal burning, appear to have had a pronounced effect on the beetle community. Local beetle diversity declined steadily towards the present day, likely due to an infilling of the forest hollow leading to a decrease in moisture level. We conclude that beetle communities are directly affected by anthropogenic intensity and land-use change. When aiming to preserve or restore natural forest conditions, recording their past changes in diversity can help guide conservation and restoration. In doing so, it is important to look back beyond the time of significant human impact, and for this, information contained in paleoecological records is irreplaceable.Peer reviewe

Technology of Microclimate Regulation in Organic and Energy-Sustainable Livestock Production

The control of climatic conditions where cattle are kept is one of the challenges in the livestock sector regarding the digital automation of the process. (1) Background: The main purpose of this study is to define the optimal foundations for automatic climatic systems in organic and energy-sustainable livestock production. In particular, the following components are suggested: (a) the determination of current deviations and interdependency between factors; (b) an algorithm for defining the possible sources of regulation; (c) the ranking approach of the optimal sequence of possible sources; and (d) ensuring transparency and coordination of the model with organic and energy certificates. (2) Methods: This investigation accumulates information on the characteristics of the main microclimatic parameters and simulates their possible combinations in a livestock building in Poland within 24 h of a spring day. A few indices are considered that signal the impact on the thermal comfort of cattle based on the example of recommended measures for the Angus steer genotype. (3) Results: The proposed transparent algorithm is designed for selecting and ranking potential sources of microclimate control according to three criteria. (4) Conclusions: This paper potentially contributes to determining the most optimal digital algorithm for managing microclimate conditions to ensure acceptable comfort for animals, meeting the requirements of organic certification with minimum costs of production, and switching to sustainable types of energy with consideration of technologies’ efficiency. The algorithm is scalable and adjustable to the individual conditions of any livestock premise with a digitally controlled environment

Millennial-scale perspective on biodiversity conservation of the forest-steppe ecotone in Europe

Paleoecological studies document that grasslands with a high conservation value in the European forest-steppe ecotone are often anthropogenically derived, requiring management to avoid woodland recovery. Nevertheless, naturally open areas often persisted within this ecotone, explaining the high richness of these grassland

Pollen-inferred quantitative reconstructions of Holocene land-cover in NW Europe for the evaluation of past climate-vegetation feedbacks III : Evaluation of the REVEALS-based reconstructions using the Czech Republic pollen database

VR LANDCLIMNordForsk LANDCLIMMERG

Synthesis and antimicrobial evaluation of 6-alkylamino-N-phenylpyrazine-2-carboxamides

This work presents synthesis and antimicrobial evaluation of nineteen 6-alkylamino-N-phenylpyrazine-2-carboxamides. Antimycobacterial activity was determined against Mycobacterium tuberculosis H37Rv, M. kansasii and two strains of M. avium. Generally, the antimycobacterial activity increased with prolongation of simple alkyl chain and culminated in compounds with heptylamino substitution (3e, 4e) with MIC = 5-10 μm against M. tuberculosis H37Rv. On the contrary, derivatives with modified alkyl chain (containing e.g. terminal methoxy or hydroxy group) as well as phenylalkylamino derivatives were mainly inactive. The most active compounds (with hexyl to octylamino substitution) were evaluated for their in vitro activity against drug-resistant strains of M. tuberculosis and possessed activity comparable to that of the reference drug isoniazid. None of the tested compounds were active against M. avium. Some derivatives exhibited activity against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (best MIC = 7.8 μm), while Gram-negative strains as well as tested fungal strains were completely unsusceptible. Active compounds were tested for in vitro toxicity on various cell lines and in most cases were non-toxic up to 100 μm.status: publishe

Preparation of Silica Nanoparticles Loaded with Nootropics and Their In Vivo Permeation through Blood-Brain Barrier

The blood-brain barrier prevents the passage of many drugs that target the central nervous system. This paper presents the preparation and characterization of silica-based nanocarriers loaded with piracetam, pentoxifylline, and pyridoxine (drugs from the class of nootropics), which are designed to enhance the permeation of the drugs from the circulatory system through the blood-brain barrier. Their permeation was compared with non-nanoparticle drug substances (bulk materials) by means of an in vivo model of rat brain perfusion. The size and morphology of the nanoparticles were characterized by transmission electron microscopy. The content of the drug substances in silica-based nanocarriers was analysed by elemental analysis and UV spectrometry. Microscopic analysis of visualized silica nanocarriers in the perfused brain tissue was performed. The concentration of the drug substances in the tissue was determined by means of UHPLC-DAD/HRMS LTQ Orbitrap XL. It was found that the drug substances in silica-based nanocarriers permeated through the blood brain barrier to the brain tissue, whereas bulk materials were not detected in the brain