Becoming Intimate With Developmental Knowledge: Pedagogical Explorations with Collective Biography

In this article, we draw on postfoundational frameworks to make visible the subjectification processes by which practitioners simultaneously master and become mastered by developmental theories. We emphasize the implication of the entire minded-body in the processes of the developmental worker formation. We show these processes through empirical investigation with data gathered through collective biography in a child development graduate course in a child and youth care program. Often developmental psychology masks itself as “just natural” knowledge that informs our practice. However, the article shows that our relationship with developmental psychology is much more intricate and intimate than we might believe. This intimate relationship can provide new possibilities for resisting developmental knowledge in practice and training

The Discourse and Culture of Chip Music Studying the Methods and Values of the Chipscene

Master's Thesis popular music MUR502 - University of Agder 2018This thesis tackles some of the complicated internal issues that has colored the chipscene. Through analyzing discourse in blog posts, academic material and online communication between chiptune practitioners it aims to define the current generation of chiptune musicians. Interviewing figures of interest has given insight into the aesthetic of chiptune and what is important to the current community. Analyzing the aesthetic values can give insight into the elements of the genre that is so difficult to define. The goal is to expand upon the work of Marylou Polymeropoulou (2014) and see it in context of my own involvement with the scene as well as the many reflections documented by academics. By gaining an understanding of how the different historical events and ideologies of chiptune culture has affected the discourse throughout the ages, we can perhaps define a new generation of chipmusicians. Most importantly, there are aspects of newer chiptune culture that has remained untouched in academia. This research aims to contribute knowledge on the aesthetics of the chiptune medium and culture, put in the context of Polymeropoulou’s work. It will demonstrate the musical aesthetic that is often neglected in other articles. For instance, the tech-oriented aesthetic created on obsolete hardware that has recently influenced those creating chiptune music with modern tools, and vice versa

Mistaking opposition for autonomy: psychophysical studies on detecting choice bias

Do people know when they act freely and autonomously versus when their actions are influenced? While the human aspiration to freedom is widespread, little research has investigated how people perceive whether their choices are biased. Here, we explored how actions congruent or incongruent with suggestions are perceived as influenced or free. Across three experiments, participants saw directional stimuli cueing left or right manual responses. They were instructed to follow the cue's suggestion, oppose it or ignore it entirely to make a 'free' choice. We found that we could bias participants' 'free responses' towards adherence or opposition, by making one instruction more frequent than the other. Strikingly, participants consistently reported feeling less influenced by cues to which they responded incongruently, even when response habits effectively biased them towards such opposition behaviour. This effect was so compelling that cues that were frequently presented with the Oppose instruction became systematically judged as having less influence on behaviour, artificially increasing the sense of freedom of choice. Taken together, these findings demonstrate that acting contrarian distorts the perception of autonomy. Crucially, we demonstrate the existence of a novel illusion of freedom evoked by trained opposition. Our results have important implications for understanding mechanisms of persuasion

Kitchen cloths: Consumer practices, drying properties and bacterial growth and survival

Kitchen cloths have an important role in maintaining kitchen surfaces hygienically clean but may also act as vehicles for cross contamination of pathogens from food spills to hands and other foods. The aim of the present study was to map consumer practices across Europe and identify main factors that may contribute to unsafe use of kitchen cloths, such as type of cloth and storage practices. Consumer practices related to cloths were investigated in a web-based survey (N = 2394), while drying properties and growth and survival of Salmonella, Campylobacter and non-pathogenic food associated bacteria in inoculated cloths were studied in laboratory tests mimicking consumer practices. Among consumers in six European countries, cotton and microfiber cloths were reported to be the most used cloth types for cleaning food preparation areas and wiping up spills. . Fifty-seven percent of the consumers reportedly hang the cloth to dry after use. A large majority (72%) changed their cloths at regular times, with an average reported frequency of every 6 days. Large variations in water absorption (63–201 g) and drying rate (31.8–99.8% water loss after 4.5 h) among 17 types of commercially available cloths were found. Hanged up cloths dried faster than cloths stored crumpled as balls. Salmonella multiplied in all types of new cloths that were not hung to dry (crumpled), but about 3 log reduction or more were found after hanging cloths to dry for 24 h. For cloths collected from consumers, growth of inoculated Salmonella was not observed, but hanging the cloths resulted in more than 3 log reduction in numbers. A large variation in survival of Campylobacter was found depending on the type of cloth, but more than 5 log reduction was found after 24 h in all hanging cloths. A polypropylene and a viscose cloth with low water uptake and fast drying appeared to be the safest choice with a rapid reduction of both pathogens when hung (respectively 2 log and >6 log reduction for Salmonella and Campylobacter after 4.5 h) and reduction of Campylobacter when stored crumpled. The least safe cloth regarding pathogen growth and survival was a knitted cotton cloth with high water uptake and slow drying. There was no systematic difference in growth and survival of bacteria between microfiber cloths and cloths of other materials, nor between cloths with and without antimicrobial compounds. The present study shows that 16% of consumers have practices that would allow pathogens to contaminate, grow and survive in cloths until the next use. Touching and using these cloths can lead to contamination of hands and food contact surfaces, and potentially to ingestion of pathogens. Consumers should be advised to change cloths after using them for meat spills, but also to choose cloths that dry fast and keep them hanging to dry between use.publishedVersio

Waste free construction site - A buzzword, nice to have or more

The building and construction industry is responsible for up to 25% of the total waste generated globally. Most construction sites in Norway on average generate 40–60 kg waste per gross floor area built and the average material recovery rate is ca. 46%. Existing requirements focus on waste sorting as a measure to increase material recovery rates. There are on-going national activities with an ambition to achieve waste free construction sites. However, there is lack of a common definition, standard and transparent data collection, and reporting system. This study presents a method for the evaluation and follow-up of construction waste and the associated greenhouse gas (GHG) emissions. The methodology was tested using the actual construction waste data collected from 36 Norwegian building cases to evaluate the quantity of construction waste, waste-related GHG emissions per building typology, sorting grade and waste recycling rate. The buildings in total generated ca. 7800 tonnes of waste and ca.12900 tonnes CO2eq and on average ca. 51 kg/m2 waste and 88kgCO2eq/m2. The building projects had a high average sorting grade (89%) and a low average recycling rate (32%). Gypsum, mixed wood, clean wood, and mixed waste are the top waste fractions representing ca. 56% of the total waste volume. This highlights there is still a long way to go to achieve waste free construction sites ambitions. The results also suggest the need for using transparent data collection and communication methods, collaboration in the value chain, stricter regulations, and incentives for encouraging the development of new and existing waste prevention solutions and technologies.publishedVersio

Systemic sclerosis is associated with specific alterations in gastrointestinal microbiota in two independent cohorts.

ObjectiveTo compare faecal microbial composition in patients with systemic sclerosis (SSc) from 2 independent cohorts with controls and to determine whether certain genera are associated with SSc-gastrointestinal tract (GIT) symptoms.DesignAdult patients with SSc from the University of California, Los Angeles (UCLA) and Oslo University Hospital (OUH) and healthy controls participated in this study (1:1:1). All participants provided stool specimens for 16S rRNA sequencing. Linear discriminant analysis effect size demonstrated genera with differential expression in SSc. Differential expression analysis for sequence count data identified specific genera associated with GIT symptoms as assessed by the GIT 2.0 questionnaire.ResultsThe UCLA-SSc and OUH-SSc cohorts were similar in age (52.1 and 60.5 years, respectively), disease duration (median (IQR): 6.6 (2.5-16.4) and 7.0 (1.0-19.2) years, respectively), gender distribution (88% and 71%, respectively), and GIT symptoms (mean (SD) total GIT 2.0 scores of 0.7 (0.6) and 0.6 (0.5), respectively). Principal coordinate analysis illustrated significant microbial community differences between SSc and controls (UCLA: p=0.001; OUH: p=0.002). Patients with SSc had significantly lower levels of commensal genera deemed to protect against inflammation, such as Bacteroides (UCLA and OUH), Faecalibacterium (UCLA), Clostridium (OUH); and significantly higher levels of pathobiont genera, such as Fusobacterium (UCLA), compared with controls. Increased abundance of Clostridium was associated with less severe GIT symptoms in both cohorts.ConclusionsThe present analysis detected specific aberrations in the lower GIT microbiota of patients with SSc from 2 geographically and ethnically distinct cohorts. These findings suggest that GIT dysbiosis may be a pathological feature of the SSc disease state

Gut microbiota-dependent trimethylamine N-oxide associates with inflammation in common variable immunodeficiency

A substantial proportion of patients with common variable immunodeficiency (CVID) have inflammatory and autoimmune complications of unknown etiology. We have previously shown that systemic inflammation in CVID correlates with their gut microbial dysbiosis. The gut microbiota dependent metabolite trimethylamine N-oxide (TMAO) has been linked to several metabolic and inflammatory disorders, but has hitherto not been investigated in relation to CVID. We hypothesized that TMAO is involved in systemic inflammation in CVID. To explore this, we measured plasma concentrations of TMAO, inflammatory markers, and lipopolysaccharide (LPS) in 104 CVID patients and 30 controls. Gut microbiota profiles and the bacterial genes CutC and CntA, which encode enzymes that can convert dietary metabolites to trimethylamine in the colon, were examined in fecal samples from 40 CVID patients and 86 controls. Furthermore, a food frequency questionnaire and the effect of oral antibiotic rifaximin on plasma TMAO concentrations were explored in these 40 patients. We found CVID patients to have higher plasma concentrations of TMAO than controls (TMAO 5.0 [2.9–8.6] vs. 3.2 [2.2–6.3], p = 0.022, median with IQR). The TMAO concentration correlated positively with tumor necrosis factor (p = 0.008, rho = 0.26), interleukin-12 (p = 0.012, rho = 0.25) and LPS (p = 0.034, rho = 0.21). Dietary intake of meat (p = 0.678), fish (p = 0.715), egg (p = 0.138), dairy products (p = 0.284), and fiber (p = 0.767) did not significantly impact on the TMAO concentrations in plasma, nor did a 2-week course of the oral antibiotic rifaximin (p = 0.975). However, plasma TMAO concentrations correlated positively with gut microbial abundance of Gammaproteobacteria (p = 0.021, rho = 0.36). Bacterial gene CntA was present in significantly more CVID samples (75%) than controls (53%), p = 0.020, potentially related to the increased abundance of Gammaproteobacteria in these samples. The current study demonstrates that elevated TMAO concentrations are associated with systemic inflammation and increased gut microbial abundance of Gammaproteobacteria in CVID patients, suggesting that TMAO could be a link between gut microbial dysbiosis and systemic inflammation. Gut microbiota composition could thus be a potential therapeutic target to reduce systemic inflammation in CVID

A disease-specific decline of the relative abundance of Bifidobacterium in patients with autoimmune hepatitis

BACKGROUND:The pathogenesis of autoimmune hepatitis (AIH) is poorly understood and little is known about enteric microbiota in AIH. AIM:To investigate disease-specific microbiome alterations in AIH. METHODS:The V1-V2 variable regions of the 16S rRNA gene were sequenced in faecal samples from 347 patients with AIH and controls (AIH n = 72, healthy controls (HC) n = 95, primary biliary cholangitis (PBC) n = 99 and ulcerative colitis (UC) n = 81). RESULTS:Biodiversity (Shannon entropy) was decreased in AIH patients compared to HC (P = 0.016), which was partially reversed by azathioprine (P = 0.011). Regarding between-sample diversity, AIH patients separated from HC, PBC and UC individuals (all P = 0.001). Compared to HC, decreased relative abundance of anaerobic genera such as Faecalibacterium and an increase of Veillonella and the facultative anaerobic genera Streptococcus and Lactobacillus were detected. Importantly, a disease-specific decline of relative abundance of Bifidobacterium was observed in AIH patients. Lack of Bifidobacterium was associated with failure to achieve remission of AIH (P < 0.001). Of potential therapeutic implication, Bifidobacterium abundance correlated with average protein intake (P < 0.001). Random forests classification between AIH and PBC on the microbiome signature yielded an area under receiver operating characteristic curve (AUC) of 0.787 in the training cohort, and an AUC of 0.849 in an external validation cohort. CONCLUSION:Disease-specific faecal microbial alterations were identified in patients with AIH. Intestinal dysbiosis in AIH was characterised by a decline of Bifidobacterium, which was associated with increased disease activity. These results point to the contribution of intestinal microbiota to AIH pathogenesis and to novel therapeutic targets

Clinical and biochemical impact of vitamin B6 deficiency in primary sclerosing cholangitis before and after liver transplantation

Background and aims We previously demonstrated that people with primary sclerosing cholangitis (PSC) had reduced gut microbial capacity to produce active vitamin B6 (pyridoxal 5’-phosphate [PLP]), which corresponded to lower circulating PLP levels and poor outcomes. Here, we define the extent and biochemical and clinical impact of vitamin B6 deficiency in people with PSC from several centers before and after liver transplantation (LT). Methods We used targeted liquid chromatography-tandem mass spectrometry to measure B6 vitamers and B6-related metabolic changes in blood from geographically distinct cross-sectional cohorts totaling 373 people with PSC and 100 healthy controls to expand on our earlier findings. Furthermore, we included a longitudinal PSC cohort (n = 158) sampled prior to and serially after LT, and cohorts of people with inflammatory bowel disease (IBD) without PSC (n = 51) or with primary biliary cholangitis (PBC) (n = 100), as disease controls. We used Cox regression to measure the added value of PLP to predict outcomes before and after LT. Results In different cohorts, 17-38% of people with PSC had PLP levels below the biochemical definition of a vitamin B6 deficiency. The deficiency was more pronounced in PSC than in IBD without PSC and PBC. Reduced PLP was associated with dysregulation of PLP-dependent pathways. The low B6 status largely persisted after LT. Low PLP independently predicted reduced LT-free survival in both non-transplanted people with PSC and in transplant recipients with recurrent disease. Conclusions Low vitamin B6 status with associated metabolic dysregulation is a persistent feature of PSC. PLP was a strong prognostic biomarker for LT-free survival both in PSC and recurrent disease. Our findings suggest that vitamin B6 deficiency modifies the disease and provides a rationale for assessing B6 status and testing supplementation. Impact and implications We previously found that people with PSC had reduced gut microbial potential to produce essential nutrients. Across several cohorts, we find that the majority of people with PSC are either vitamin B6 deficient or have a marginal deficiency, which remains prevalent even after liver transplantation. Low vitamin B6 levels strongly associate with reduced liver transplantation-free survival as well as deficits in biochemical pathways dependent on vitamin B6, suggesting that the deficiency has a clinical impact on the disease. The results provide a rationale for measuring vitamin B6 and to investigate whether vitamin B6 supplementation or modification of the gut microbial community can help improve outcomes for people with PSC.publishedVersio