Proteomic analysis of immediate-early response plasma proteins after 70% and 90% partial hepatectomy

AIM: Partial hepatectomy (PH) induces robust hepatic regenerative and metabolic responses that are considered to be triggered by humoral factors. The aim of the study was to identify plasma protein factors that potentially trigger or reflect the body's immediate-early responses to liver mass reduction. METHODS: Male C57BL/6 mice were subjected to sham operation, 70% PH or 90% PH. Blood was collected from the inferior vena cava at 20, 60 and 180 min after surgery. RESULTS: Using a label-free quantitative mass spectrometry-based proteomics approach, we identified 399 proteins exhibiting significant changes in plasma expression between any two groups. Of the 399 proteins, 167 proteins had multiple unique sequences and high peptide ID confidence (>90%) and were defined as priority 1 proteins. A group of plasma proteins largely associated with metabolism is enriched after 70% PH. Among the plasma proteins that respond to 90% PH are a dominant group of proteins that are also associated with metabolism and one known cytokine (platelet factor 4). Ninety percent PH and 70% PH induces similar changes in plasma protein profile. CONCLUSION: Our findings enable us to gain insight into the immediate-early response of plasma proteins to liver mass loss. Our data support the notion that increased metabolic demands of the body after massive liver mass loss may function as a sensor that calibrates hepatic regenerative response

Constructing Z-scheme LaTiO\u3csub\u3e2\u3c/sub\u3eN/g-C\u3csub\u3e3\u3c/sub\u3eN\u3csub\u3e4\u3c/sub\u3e@Fe\u3csub\u3e3\u3c/sub\u3eO\u3csub\u3e4\u3c/sub\u3e magnetic nano heterojunctions with promoted charge separation for visible and solar removal of indomethacin

© 2020 Elsevier Ltd Pharmaceutical effluents in water bodies pose hazards to the ecosystem because of their potent biological toxicity. Focusing on the removal of such toxic complicated pharmaceutical residues, an innovative LaTiO2N/g-C3N4@Fe3O4 heterojunction photocatalyst was assembled by a simplistic route for visible and solar light degradation of anti-inflammatory drug indomethacin (IDM). The LCF-20 catalyst (with LaTiO2N:g-C3N4 -0.2:1) shows excellent performance for visible light photodegradation of IDM, as evidenced by 97.3 % removal in just 45 min exposure which is about 13 times faster than bare g-C3N4. 83.4 % of total organic carbon removal was achieved by LVF-20 under visible light. Also, with natural sunlight, nearly 80 % of IDM was removed in 90 min irradiation. The heterojunction\u27s extensive intimate interfacial interactions amid LaTiO2N and g-C3N4 reduce the shortcomings of the two for a better photo-activity. The high visible activity, diminished recombination, high charge transfer is attributed to effective Z-scheme transfer facilitated by Fe3O4 nanoparticles. Scavenging experiments prove the importance of superoxide radicals as the dominant species responsible for photodegradation reaction. By mass spectrometry and total organic carbon analysis, a reaction mechanism was also reasonably proposed. The photocatalytic mechanism was discussed in light of conventional and Z-scheme transfer for better insight. The catalyst is stable, recyclable and magnetically separable. This investigation offers a new perspective in the rational design and manufacture of organic-inorganic nitrides based magnetically recoverable heterojunctions as LaTiO2N/g-C3N4@Fe3O4. Such heterojunctions present a new class of robust hierarchical photocatalytic materials which are capable of remediation of pharmaceutical residues under practical conditions

Can customer sentiment impact firm value? An integrated text mining approach

Developing measures to capture customer sentiment and securing a positive customer experience is a strategic necessity to improve firm profitability and shareholder value. The paper considers the relationship between customer satisfaction, earnings, and firm value as these drives change in stock prices, customer, and investor sentiment. The present study investigates the impact of customer sentiment polarity on stock prices based on Indian automobile sector databased such as the Indian Nifty Auto SNE (Maruti Suzuki, Tata Motors, and Eicher). A top-down approach is adopted to construct a financial proxy-based sentiment index completed with sentiment extracted from automobile news and customer reviews. The paper uses a text mining approach to holistically measure customer sentiment’s impact on investor sentiment and stock prices. The study was initially performed at the overall individual stock from the Nifty Auto NSE but focused on the top three passenger vehicle manufacturing companies i.e., Maruti Suzuki, Tata Motors, and Eicher. It was found that the sentiment index was augmented with news and customer reviews allows predicting more accurately NIFTY AUTO stock price movements. This implies that customer sentiment is a major driver of investor sentiment which in turn impacts the stock market and the firm value. Thus, the present study is an integrated approach to holistically measure customer sentiment’s impact on investor sentiment and stock prices.WOS:00071138140002

Designing of bentonite based nanocomposite hydrogel for the adsorptive removal and controlled release of ampicillin

© 2020 In pharmacy, semisynthetic antibiotics with beta-lactam ring are the most prominently used drugs. The use of these drugs for humans and animals is continuously expanding. Their presence in the water system even at low concentrations can prove to be fatal to living beings. Also, they can even grow antibiotic-resistant bacteria and thus elimination of such drugs becomes very essential. Our study is focused on batch experiments for adsorptive removal of ampicillin (AMP) and its cumulative release in different solutions using xanthan gum-cl-poly(itaconic acid)/bentonite (XG-cl-poly(IA)/BN) nanocomposite hydrogel. It was synthesized by facile microwave method. The adsorption data of AMP was analyzed using various isotherm models such as Langmuir, Freundlich, Temkin and kinetic models such as Pseudo-first order, Pseudo-second order and Intraparticle diffusion. The maximum adsorption capacity as determined from Langmuir model was 245.09 mg/g at 318 K and solution pH 7. Also, XG-cl-poly(IA)/BN nanocomposite hydrogel was evaluated for AMP release in distilled water and at different pH solutions (2.2, 5.4, 7.4 and 9.4). The maximum AMP release was observed at pH 2.2 (37%)

Electrocatalytic Oxidation of Ascorbic Acid Using a Poly(aniline-co-m-ferrocenylaniline) Modified Glassy Carbon Electrode

A poly(aniline-co-m-ferrocenylaniline) was successfully synthesized on a glassy carbon electrode (GCE) by electrochemical copolymerization using a scan potential range from −0.3 to +0.9 V (vs. Ag/AgCl) in 0.5 M H2SO4 containing 30% acetonitrile (ACN), 0.1 M aniline (Ani) and 0.005 M m-ferrocenyaniline (m-FcAni). The field emission scanning electron microscope (FESEM) and electrochemical methods were used to characterize the poly(Ani-co-m-FcAni) modified electrode. The poly(Ani-co-m-FcAni)/GCE exhibited excellent electrocatalytic oxidation of ascorbic acid (AA) in citrate buffer solution (CBS, pH 5.0). The anodic peak potential of AA was shifted from +0.55 V at the bare GCE to +0.25 V at the poly(Ani-co-m-FcAni)/GCE with higher current responses than those seen on the bare GCE. The scan number at the 10th cycle was selected as the maximum scan cycle in electrochemical polymerization. The limit of detection (LOD) was estimated to be 2.0 μM based on the signal-to-noise ratio (S/N = 3). The amperometric responses demonstrated an excellent selectivity for AA determination over glucose (Glu) and dopamine (DA)

Construction of dual Z-scheme g-C3N4/Bi4Ti3O12/Bi4O5I2 heterojunction for visible and solar powered coupled photocatalytic antibiotic degradation and hydrogen production: Boosting via I−/I3− and Bi3+/Bi5+ redox mediators

© 2020 Elsevier B.V. Inspired by waste to energy production, we report construction of dual Z-scheme advanced photocatalyst g-C3N4/Bi4Ti3O12/Bi4O5I2 heterojunction for coupled photocatalytic H2 evolution and degradation of antibiotics with high efficiency. The optimal CTBT-30 i.e (40 %g-C3N4/Bi4Ti3O12)/30 % Bi4O5I2 photocatalyst exhibited an excellent rate of H2 production under visible light (56.2 mmol g−1 h−1) along with simultaneous 87.1 % ofloxacin (OFL) removal. The H2 production rate is manifolds higher than in ultrapure water, sulfadiazine, rhodamine B and higher in hole scavenging triethanolamine. The interfacial intimate coupling with well-matched energy bands, foster the charge separation with effective Z-scheme transfer facilitated by I3−/I− and Bi3+/Bi5+ and redox mediators. The scavenging of majority of holes for direct oxidation or via [rad]OH radical formation leaves photogenerated electrons (at CB of g-C3N4 and Bi4O5I2) free for H2 evolution from H2O. Such work is promising for designing high photo-absorbing heterojunction photocatalysts for dual functionalities of clean energy production and environmental detoxification