Analysis of risk factors in an indirect distribution channel

Purpose: The article aims to present an analysis of risk factors in an indirect distribution channel. Design/Methodology/Approach: The research methodology used are indicators that enable the analysis of risk factors in the distribution channel. The results obtained by the FMEA method enabled the use of the preventive mechanisms method in the enterprise. The purpose of using the FMEA method is to characterize the most dangerous risk factors in the surveyed entities, which will be most felt by the organization in enterprises. Findings: Numerous risk factors can arise in a network of indirect distribution channels. The applied FMEA method identified which risk factors are the most dangerous for the organization regarding probability, detectability, and significance of the risk factor. The results obtained from the FMEA method made it possible to develop preventive mechanisms for the examined company and thus reduce the possibility of risk factors. Practical Implications: Over time, new risk factors may appear, so you should constantly monitor and analyze disturbances using the FMEA method that allows you to manage risk factors. Enterprises that are part of an indirect distribution channel should develop preventive mechanisms to ensure an efficiently functioning organization. Originality/value: The studies showed that the magnitude of the effect and the probability of risk factors depend on the first activity performed in the distribution network up to the last link of the supply chain. It is also influenced by the continuity of the consequences of a given occurrence of a risk factor. The obtained data is the basis for risk management for distribution entities.peer-reviewe

The SERRATE protein is involved in alternative splicing in <em>Arabidopsis thaliana</em>

Howalternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcript-ase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 50 splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not cor-respond to the changes observed in the se-1mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1andDCL1, and is similar to the regu-lation of AS in which CBC is involved

Plasmid pP62BP1 isolated from an Arctic Psychrobacter sp. strain carries two highly homologous type II restriction-modification systems and a putative organic sulfate metabolism operon

The complete nucleotide sequence of plasmid pP62BP1 (34,467 bp), isolated from Arctic Psychrobacter sp. DAB_AL62B, was determined and annotated. The conserved plasmid backbone is composed of several genetic modules, including a replication system (REP) with similarities to the REP region of the iteron-containing plasmid pPS10 of Pseudomonas syringae. The additional genetic load of pP62BP1 includes two highly related type II restriction-modification systems and a set of genes (slfRCHSL) encoding enzymes engaged in the metabolism of organic sulfates, plus a putative transcriptional regulator (SlfR) of the AraC family. The pP62BP1 slflocus has a compact and unique structure. It is predicted that the enzymes SlfC, SlfH, SlfS and SlfL carry out a chain of reactions leading to the transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible starting substrate. Comparative analysis of the nucleotide sequences of pP62BP1 and other Psychrobacter spp. plasmids revealed their structural diversity. However, the presence of a few highly conserved DNA segments in pP62BP1, plasmid 1 of P. cryohalolentis K5 and pRWF-101 of Psychrobacter sp. PRwf-1 is indicative of recombinational shuffling of genetic information, and is evidence of lateral gene transfer in the Arctic environment