Promoção de Inclusão Produtiva com Segurança Sanitária, um relato de experiência / Promotion of Productive Inclusion with Health Security, an experience report

Para trabalhar com alimentos, é preciso respeitar as normas sanitárias com o objetivo de evitar a contaminação dos produtos e zelar pelo bem-estar e saúde dos consumidores. Como o município de Terenos/MS, possui 21 assentamentos rurais e nossa população é 58% rural, buscamos através deste projeto, auxiliar e apoiar a produção de alimentos da agricultura familiar, baseado nos princípios de inclusão social e econômica, tendo como base a RDC  49/2013. Pois os agricultores familiares enfrentavam muitos entraves em relação às normas sanitárias, o que atrapalhavam ou dificultavam a sua produção. Auxiliamos este público nas áreas de qualificação profissional, empreendedorismo e regularização junto à Vigilância Sanitária, para que estes sejam fortalecidos e que ocorra uma ampliação dos seus produtos no mercado consumidor. Mostramos para estes que adotando as boas práticas na sua produção possibilita fazer produtos de qualidade em um ambiente às vezes não tão grande e estruturado como exige a legislação sanitária. Foram realizadas reuniões e cursos nos assentamentos da região com o intuito destes produtores conhecerem quais são as ações da Vigilância Sanitária, e principalmente quebrar o paradigma de que a Vigilância Sanitária “só dá prejuízo”, além de ajudá-los a saírem da informalidade e regularizar as atividades desenvolvidas por estes juntos aos órgãos de fiscalização. Foram desenvolvidos o Curso de Boas Práticas de Manipulação, Cadastro do Produtor e dos produtos , Carteira de Sanitária ou de Saúde , Confecção do Rótulo (etiqueta) com código de barra  sem custo para o produtor – Parceria com a AGRAER (Agência de Desenvolvimento Agrário e Extensão Rural), para a comercialização dos produtos produzidos pela agricultura familiar, visando a obtenção de renda, fortalecimento e ampliação da entrada de produtos no mercado consumidor. Em quase três anos de projeto, passamos de 24 para 80 produtores cadastrados e comercializando seus produtos, que vão de doces e hortifrutigranjeiros.  A partir do momento que nós Vigilância Sanitária, passarmos a orientar mais e não só punir, ou seja, abandonarmos o modelo de fiscalização higienista e objetivamos o controle do risco, conseguiremos uma produção mais segura e diminuiremos os riscos de uma contaminação dos alimentos. 

CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY

It has been possible to show by electron microscopy of samples selected from sucrose gradients that particles of specific size and shape are present in supernatant fluids derived from nucleated animal and plant cells, but not in extracts from Escherichia coli. Aminoacyl transferase I activity in these same gradients sediments in two peaks representing material of approximately 5–7S and 18–20S. A rectangular particle, 100 x 145 A in size, sediments at 19S and coincides with the second peak of transferase I activity. The possibility that the rectangular particle may be a "carrier" particle associated with transferase I is discussed

THE IN VIVO PROTEIN SYNTHETIC ACTIVITIES OF FREE VERSUS MEMBRANE-BOUND RIBONUCLEOPROTEIN IN A PLASMA-CELL TUMOR OF THE MOUSE

Cytoplasmic extracts of the transplantable RPC-20 plasma-cell tumor were fractionated by sucrose density gradient centrifugation. Four major fractions were distinguished: (a) microsomes and mitochondria; (b) membrane-free polyribosomes; (c) free monomeric ribosomes; and (d) soluble fraction. The fractions were analyzed for RNA and lipid phosphorus, and their particulate components were characterized by electron microscopy. Particular attention was paid to the problem of membrane contamination of the free polyribosome fraction. It was shown that this contamination was small in relation with the total content of ribosomes in the fraction, and that it consisted primarily of smooth-surfaced membranes which were not physically associated with the polyribosomes themselves. In vivo incorporation studies were carried out by injecting tumor-bearing animals intravenously with leucine-C14, removing the tumors at various times thereafter, and determining the distribution of protein radioactivity among the gradient-separated cytoplasmic fractions. The free polyribosome and the microsome-mitochondria fractions constituted active centers for protein synthesis. It was shown that nascent protein of the free polyribosome fractions was not associated significantly with the contaminating membranes. The kinetics of labeling during incorporation times up to 11 min suggested that protein synthesized on the free polyribosomes was rapidly transferred in vivo to the soluble fraction of the cell, while protein synthesized by the microsomes and mitochondria remained localized within these elements. It was estimated that the free polyribosome fraction and the microsome-mitochondria fraction accounted for approximately equal proportions of the total cytoplasmic protein synthesis in vivo

IAP Display: A Simple Method to Identify Mouse Strain Specific IAP Insertions

Intracisternal A-type particle (IAP) elements are high copy number long terminal repeat (LTR) rodent retrotransposons. Some IAP elements can transpose, and are responsible for ~12% of spontaneous mouse mutations. Inbred mouse strains show variation in genomic IAP distribution, contributing to inter-strain genetic variability. Additionally IAP elements can influence the transcriptional regulation of neighbouring genes through their strong LTR promoter, effecting phenotypic variation. This genetic and phenotypic variability can translate into experimental variability between mouse strains. For example, it has been demonstrated that strain-specific genetic/epigenetic factors can interact to yield variable responses to drugs. Therefore, in experimental contexts it is essential to unequivocally identify mouse strains. Recently it was estimated that any two inbred strains share only ~40% of their IAP insertions. Of the remaining 60%, some insertions will be strain specific, fixed during inbreeding. These fixed insertions can be exploited as genetic markers to identify inbred strains, if they can be identified simply and efficiently. Here, we report the development of a PCR-based system allowing direct acquisition of strain-specific IAP insertions. In a pilot study, we identified 21 IAP loci, genotyped IAP insertions at 9 loci, and discovered two strain-specific insertions that could reliably identify these strains

Defending the genome from the enemy within:mechanisms of retrotransposon suppression in the mouse germline

The viability of any species requires that the genome is kept stable as it is transmitted from generation to generation by the germ cells. One of the challenges to transgenerational genome stability is the potential mutagenic activity of transposable genetic elements, particularly retrotransposons. There are many different types of retrotransposon in mammalian genomes, and these target different points in germline development to amplify and integrate into new genomic locations. Germ cells, and their pluripotent developmental precursors, have evolved a variety of genome defence mechanisms that suppress retrotransposon activity and maintain genome stability across the generations. Here, we review recent advances in understanding how retrotransposon activity is suppressed in the mammalian germline, how genes involved in germline genome defence mechanisms are regulated, and the consequences of mutating these genome defence genes for the developing germline

Negotiating Free Will: Hypnosis and Crime in Early Twentieth-Century Germany

The history of free will has yet to be written. With few exceptions, the literature on the subject is dominated by legal and philosophical works, most of which recount the ideas of prominent thinkers or discuss hypothetical questions far removed from specific historical contexts. The following article seeks to redress the balance by tracing the debate on hypnosis in Germany from 1894 to 1936. Examining responses to hypnosis is tantamount to recording common understandings of autonomy and heteronomy, self-control and mind control, free will and automaticity. More specifically, it is possible to identify distinct philosophical positions related to the question as to whether hypnosis could surmount free will or not. The article demonstrates that the discourse often centred on the perceived struggle, located within a particular personality', between an individual's character' or soul' and the infiltration by a foreign or hostile force. While one group (compatibilists) emphasized the resilience of the moral inhibitions', another group (determinists) doubted that these were sufficient to withstand hypnosis

Genome-Wide Assessments Reveal Extremely High Levels of Polymorphism of Two Active Families of Mouse Endogenous Retroviral Elements

Endogenous retroviral elements (ERVs) in mice are significant genomic mutagens, causing ∼10% of all reported spontaneous germ line mutations in laboratory strains. The majority of these mutations are due to insertions of two high copy ERV families, the IAP and ETn/MusD elements. This significant level of ongoing retrotranspositional activity suggests that inbred mice are highly variable in content of these two ERV groups. However, no comprehensive genome-wide studies have been performed to assess their level of polymorphism. Here we compared three test strains, for which sufficient genomic sequence is available, to each other and to the reference C57BL/6J genome and detected very high levels of insertional polymorphism for both ERV families, with an estimated false discovery rate of only 0.4%. Specifically, we found that at least 60% of IAP and 25% of ETn/MusD elements detected in any strain are absent in one or more of the other three strains. The polymorphic nature of a set of 40 ETn/MusD elements found within gene introns was confirmed using genomic PCR on DNA from a panel of mouse strains. For some cases, we detected gene-splicing abnormalities involving the ERV and obtained additional evidence for decreased gene expression in strains carrying the insertion. In total, we identified nearly 700 polymorphic IAP or ETn/MusD ERVs or solitary LTRs that reside in gene introns, providing potential candidates that may contribute to gene expression differences among strains. These extreme levels of polymorphism suggest that ERV insertions play a significant role in genetic drift of mouse lines