Outpatient Physical Therapy Treatment of Patient with Spondylosis: A Case Study

Background and Purpose: In the United States low back pain (LBP) is one of the leading causes for individuals to reach out to health care professionals. LBP is often associated with a diagnosis of spondylosis. Spondylosis is present in about 80% of peoples in the United States. The prevalence of spondylosis continues to increase without a definite cure or form of treatment. Overall, exercise and intervention have proven to decrease symptoms of pain of those with spondylosis. However, success of treatment may see large variables in success. Further research into specific treatment interventions may be warranted to decrease chronic spondylosis in the United States. Case Description: This case involves a 72-year-old male diagnosed with spondylosis. The patient is a retired Top Gun Pilot for the United States Airforce and he participates in regular golf tournaments for recreation. He lives in a home with his wife. He began to feel pain in his low back when playing golf, walking up an incline, and bending over to carry groceries inside his home. He received imaging and was given the diagnosis of spondylosis and degenerative disc disease. Shortly after, he was referred to physical therapy for treatment of his symptoms. Intervention: Following evaluation and examination, directional preference was identified as well as mobility and strength deficits. Interventions and treatment were designed and implemented to compliment directional preferences and address strength DocuSign Envelope ID: 5958519C-66F8-46B0-8B47-200288084252 x and mobility deficits. Interventions included, but were not limited to, increasing thoracic and lumbar mobility, posterior chain strengthening, core stability interventions, manual therapy techniques and modalities. Outcomes: The patient felt significantly better following the eight weeks of treatment. Following the plan of care, the patient expressed he felt equipped to continue with his home exercise program to eliminate or decrease a return of symptoms. He expressed noticeable differences in his symptoms as well as noticeable increases in his strength and abilities within his normal daily activities following his treatment plan of care. Discussion: While interventions and manual therapy techniques were appropriate and delivered results for this patient, some research does not support or suggest just one approach being successful for all patients diagnosed with spondylosis. Further research should be done specifically on lumbar spondylosis as well as specific interventions to treat symptoms

Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure

The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeodactylum tricornutum to short-term exposure to high light was examined and the levels of D1 protein determined immunochemically. Low light (LL) acclimated cells (40 µmol photons m−2 s−1) subjected to high light (HL, 1,250 µmol photons m−2 s−1) showed rapid induction of non-photochemical quenching (NPQ) and ca. 20-fold increase in diatoxanthin (DT) concentration. This resulted from the conversion of diadinoxanthin (DD) to DT through the activation of the DD-cycle. D1 protein levels under LL decreased about 30% after 1 h of the addition of lincomycin (LINC), a chloroplast protein synthesis inhibitor, showing significant D1 degradation and repair under low irradiance. Exposure to HL lead to a 3.2-fold increase in D1 degradation rate, whereas average D1 repair rate was 1.3-x higher under HL than LL, leading to decreased levels of D1 protein under HL. There were significant effects of both HL and LINC on P. tricornutum maximum quantum yield of PSII (Fv/Fm), showing a reduction of active PSII reaction centres. Partial recovery of Fv/Fm in the dark demonstrates the photosynthetic resilience of this diatom to changes in the light regime. P. tricornutum showed high allocation of total protein to D1 and an active D1-repair cycle to limit photoinhibition

Climate Change Impacts on Southern Ross Sea Phytoplankton Composition, Productivity, and Export

The Ross Sea, a highly productive region of the Southern Ocean, is expected to experience warming during the next century along with reduced summer sea ice concentrations and shallower mixed layers. This study investigates how these climatic changes may alter phytoplankton assemblage composition, primary productivity, and export. Glider measurements are used to force a one-dimensional biogeochemical model, which includes diatoms and both solitary and colonial forms of Phaeocystis antarctica. Model performance is evaluated with glider observations, and experiments are conducted using projections of physical drivers for mid-21st and late-21st century. These scenarios reveal a 5% increase in primary productivity by midcentury and 14% by late-century and a proportional increase in carbon export, which remains approximately 18% of primary production. In addition, scenario results indicate diatom biomass increases while P. antarctica biomass decreases in the first half of the 21st century. In the second half of the century, diatom biomass remains relatively constant and P. antarctica biomass increases. Additional scenarios examining the independent contributions of expected future changes (temperature, mixed layer depth, irradiance, and surface iron inputs from melting ice) demonstrate that earlier availability of low light due to reduction of sea ice early in the growing season is the primary driver of productivity increases over the next century; shallower mixed layer depths additionally contribute to changes of assemblage composition and export. This study further demonstrates how glider data can be effectively used to facilitate model development and simulation, and inform interpretation of biogeochemical observations in the context of climate change

Possible Role of Horizontal Gene Transfer in the Colonization of Sea Ice by Algae

Diatoms and other algae not only survive, but thrive in sea ice. Among sea ice diatoms, all species examined so far produce ice-binding proteins (IBPs), whereas no such proteins are found in non-ice-associated diatoms, which strongly suggests that IBPs are essential for survival in ice. The restricted occurrence also raises the question of how the IBP genes were acquired. Proteins with similar sequences and ice-binding activities are produced by ice-associated bacteria, and so it has previously been speculated that the genes were acquired by horizontal transfer (HGT) from bacteria. Here we report several new IBP sequences from three types of ice algae, which together with previously determined sequences reveal a phylogeny that is completely incongruent with algal phylogeny, and that can be most easily explained by HGT. HGT is also supported by the finding that the closest matches to the algal IBP genes are all bacterial genes and that the algal IBP genes lack introns. We also describe a highly freeze-tolerant bacterium from the bottom layer of Antarctic sea ice that produces an IBP with 47% amino acid identity to a diatom IBP from the same layer, demonstrating at least an opportunity for gene transfer. Together, these results suggest that the success of diatoms and other algae in sea ice can be at least partly attributed to their acquisition of prokaryotic IBP genes

Silencing of the Violaxanthin De-Epoxidase Gene in the Diatom Phaeodactylum tricornutum Reduces Diatoxanthin Synthesis and Non-Photochemical Quenching

Diatoms are a major group of primary producers ubiquitous in all aquatic ecosystems. To protect themselves from photooxidative damage in a fluctuating light climate potentially punctuated with regular excess light exposures, diatoms have developed several photoprotective mechanisms. The xanthophyll cycle (XC) dependent non-photochemical chlorophyll fluorescence quenching (NPQ) is one of the most important photoprotective processes that rapidly regulate photosynthesis in diatoms. NPQ depends on the conversion of diadinoxanthin (DD) into diatoxanthin (DT) by the violaxanthin de-epoxidase (VDE), also called DD de-epoxidase (DDE). To study the role of DDE in controlling NPQ, we generated transformants of P. tricornutum in which the gene (Vde/Dde) encoding for DDE was silenced. RNA interference was induced by genetic transformation of the cells with plasmids containing either short (198 bp) or long (523 bp) antisense (AS) fragments or, alternatively, with a plasmid mediating the expression of a self-complementary hairpin-like construct (inverted repeat, IR). The silencing approaches generated diatom transformants with a phenotype clearly distinguishable from wildtype (WT) cells, i.e. a lower degree as well as slower kinetics of both DD de-epoxidation and NPQ induction. Real-time PCR based quantification of Dde transcripts revealed differences in transcript levels between AS transformants and WT cells but also between AS and IR transformants, suggesting the possible presence of two different gene silencing mediating mechanisms. This was confirmed by the differential effect of the light intensity on the respective silencing efficiency of both types of transformants. The characterization of the transformants strengthened some of the specific features of the XC and NPQ and confirmed the most recent mechanistic model of the DT/NPQ relationship in diatoms

Shoot the Messenger: Research into mRNA Vaccinations and their Mechanisms

An mRNA vaccine is a new and upcoming technology used to combat several viruses. Because viruses rely on their host’s machinery to reproduce, it is difficult to treat viral infections with antiviral drugs. Instead, viruses are largely destroyed by the host’s own immune system. Vaccines are designed to expose a person’s immune system to live-attenuated or inactivated virus. These are unable to harm a person but allow adaptive immune defenses to develop. A search of the current literature, however, shows a shift in new mRNA technology being widely explored as an option for vaccine development. mRNA vaccines are unique because they themselves do not contain the antigens. Instead, the mRNA is used by a person’s cells to make viral proteins, which are then recognized by the immune system (Pardi et al., 2018). There are several advantages to using mRNA vaccines, such as easier production, stronger immune responses, and effectiveness against a wide variety of diseases. For example, there is not currently a vaccine for the virus that causes genital herpes. However, there is promising research suggesting that an mRNA vaccine could be effective against herpes simplex virus (Egan et al., 2020). The most recent global pandemic, caused by the SARS-CoV-2 virus, made research and production of a vaccine a top priority. The first approved and widely distributed SARS-CoV-2 vaccine was an mRNA vaccine. Recent publications have shown the ease of production of this vaccine and its high effectiveness in producing immunity against the virus that causes COVID-19 (Anderson et al., 2020). A literature review is presented that explores advancements in mRNA vaccines, future applications of this technology, and difficulties that require future research, such as optimizing storage conditions

Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: short-term versus acclimation effects

AbstractThe present study examines how different pCO2 acclimations affect the CO2- and light-dependence of photophysiological processes and O2 fluxes in four Southern Ocean (SO) key phytoplankton species. We grew Chaetoceros debilis (Cleve), Pseudo-nitzschia subcurvata (Hasle), Fragilariopsis kerguelensis (O'Meara) and Phaeocystis antarctica (Karsten) under low (160μatm) and high (1000μatm) pCO2. The CO2- and light-dependence of fluorescence parameters of photosystem II (PSII) were determined by means of a fluorescence induction relaxation system (FIRe). In all tested species, nonphotochemical quenching (NPQ) is the primary photoprotection strategy in response to short-term exposure to high light or low CO2 concentrations. In C. debilis and P. subcurvata, PSII connectivity (p) and functional absorption cross-sections of PSII in ambient light (σPSII′) also contributed to photoprotection while changes in re-oxidation times of Qa acceptor (τQa) were more significant in F. kerguelensis. The latter was also the only species being responsive to high acclimation pCO2, as these cells had enhanced relative electron transport rates (rETRs) and σPSII′ while τQa and p were reduced under short-term exposure to high irradiance. Low CO2-acclimated cells of F. kerguelensis and all pCO2 acclimations of C. debilis and P. subcurvata showed dynamic photoinhibition with increasing irradiance. To test for the role and presence of the Mehler reaction in C. debilis and P. subcurvata, the light-dependence of O2 fluxes was estimated using membrane inlet mass spectrometry (MIMS). Our results show that the Mehler reaction is absent in both species under the tested conditions. We also observed that dark respiration was strongly reduced under high pCO2 in C. debilis while it remained unaltered in P. subcurvata. Our study revealed species-specific differences in the photophysiological responses to pCO2, both on the acclimation as well as the short-term level