Moderne Bildgebung des Lungenhilus : Anatomie, Pathologien, Pitfalls

BACKGROUND Modern medical imaging is a key component of efficient in- and out-patient precision medicine. Conventional radiography and computer tomography scans (CT) are among the most frequent radiologic exams. Medical imaging plays a key role in target-oriented medicine. OBJECTIVES The purpose of this article is to review the anatomy of the hilum of the lung and its most frequent associated pathologies since it is an important gateway of elementary structures of the thorax. Important signs and patterns for image interpretation in different modalities are also reviewed. RESULTS Thorough knowledge of anatomy, signs, and patterns of pathologies especially in conventional radiography and pitfalls of the more sensitive cross-sectional imaging is essential to support target-oriented patient care. CONCLUSION Conventional radiography is affordable and readily available. It is very suitable for pathology screening, i.e., at the hilum of the lung. Cross-sectional imaging specifies diagnostics due to superior anatomic discrimination

Mass-balance reconstruction for Glacier No. 354, Tien Shan, from 2003 to 2014

This study presents a reconstruction of the seasonal mass balance of Glacier No. 354, located in the Akshiirak range, Kyrgyzstan, from 2003 to 2014. We use a distributed accumulation and temperature-index melt model driven by daily air temperature and precipitation from a nearby meteorological station. The model is calibrated with in situ measurements of the annual mass balance collected from 2011 to 2014. The snow-cover depletion pattern observed using satellite imagery provides additional information on the dynamics of mass change throughout the melting season. Two digital elevation models derived from high-resolution satellite stereo images acquired in 2003 and 2012 are used to calculate glacier volume change for the corresponding period. The geodetic mass change thus derived is used to validate the modelled cumulative glacier-wide balance. For the period 2003–12 we find a cumulative mass balance of –0.40±10mw.e.a-1. This result agrees well with the geodetic balance of –0.48±0.07mw.e.a-1over the same period

Constitutive Cytokine mRNAs Mark Natural Killer (NK) and NK T Cells Poised for Rapid Effector Function

Natural killer (NK) and NK T cells are tissue lymphocytes that secrete cytokines rapidly upon stimulation. Here, we show that these cells maintain distinct patterns of constitutive cytokine mRNAs. Unlike conventional T cells, NK T cells activate interleukin (IL)-4 and interferon (IFN)-γ transcription during thymic development and populate the periphery with both cytokine loci previously modified by histone acetylation. Similarly, NK cells transcribe and modify the IFN-γ gene, but not IL-4, during developmental maturation in the bone marrow. Lineage-specific patterns of cytokine transcripts predate infection and suggest evolutionary selection for invariant but distinct types of effector responses among the earliest responding lymphocytes

Behavioral Voluntary and Social Bioassays Enabling Identification of Complex and Sex-Dependent Pain-(-Related) Phenotypes in Rats with Bone Cancer

Simple Summary Bone metastases are one of the most common complications in patients with advanced cancer that result in pain, which is usually severe, thereby significantly reducing the patient's quality of life. Although preclinical pain research in rodents is improving, the pain phenotyping methods currently used have been criticized. This study aimed to identify in detail pain phenotypes of cancer-induced bone pain (CIBP) in both sexes of rats. CIBP in the splint bone on one side results in a distinct CIBP-related phenotype characterized by mechanical hypersensitivity, resting pain, and antalgic gait in both sexes. Progression of tumor growth leads to the establishment of the CIBP phenotype that appears earlier in male than in female rats and affects rat-specific social behaviors in both sexes. We demonstrate social transfer of pain in a bone cancer model in both sexes, resulting in mechanical and, in females, also heat hypervigilance in non-tumor bearing control rats. Cancer-induced bone pain (CIBP) is a common and devastating symptom with limited treatment options in patients, significantly affecting their quality of life. The use of rodent models is the most common approach to uncovering the mechanisms underlying CIBP; however, the translation of results to the clinic may be hindered because the assessment of pain-related behavior is often based exclusively on reflexive-based methods, which are only partially indicative of relevant pain in patients. To improve the accuracy and strength of the preclinical, experimental model of CIBP in rodents, we used a battery of multimodal behavioral tests that were also aimed at identifying rodent-specific behavioral components by using a home-cage monitoring assay (HCM). Rats of all sexes received an injection with either heat-deactivated (sham-group) or potent mammary gland carcinoma Walker 256 cells into the tibia. By integrating multimodal datasets, we assessed pain-related behavioral trajectories of the CIBP-phenotype, including evoked and non-evoked based assays and HCM. Using principal component analysis (PCA), we discovered sex-specific differences in establishing the CIBP-phenotype, which occurred earlier (and differently) in males. Additionally, HCM phenotyping revealed the occurrence of sensory-affective states manifested by mechanical hypersensitivity in sham when housed with a tumor-bearing cagemate (CIBP) of the same sex. This multimodal battery allows for an in-depth characterization of the CIBP-phenotype under social aspects in rats. The detailed, sex-specific, and rat-specific social phenotyping of CIBP enabled by PCA provides the basis for mechanism-driven studies to ensure robustness and generalizability of results and provide information for targeted drug development in the future

Evaluation of a structured treatment discontinuation in patients with inoperable alveolar echinococcosis on long-term benzimidazole therapy: A retrospective cohort study.

OBJECTIVES Alveolar echinococcosis (AE) is an orphan zoonosis of increasing concern in endemic areas, including Europe. It frequently presents in an advanced, inoperable stage, that requires life-long parasitostatic benzimidazole therapy. In some patients, long-term therapy leads to negative anti-Em18 antibody ELISA and PET. It is disputed, whether these patients are truly cured and treatment can be safely discontinued. Our aim was to retrospectively assess long-term outcome of 34 patients with inoperable AE who participated in a previous study to determine feasibility of benzimidazole treatment cessation. METHODS Retrospective analysis of medical charts was undertaken in all 34 AE patients who participated in our previous study. Of particular interest were AE recurrence or other reasons for re-treatment in patients who stopped benzimidazole therapy and whether baseline clinical and laboratory parameters help identify of patients that might qualifiy for treatment cessation. Additionally, volumetric measurement of AE lesions on contrast-enhanced cross-sectional imaging was performed at baseline and last follow-up in order to quantify treatment response. RESULTS 12 of 34 patients stopped benzimidazole therapy for a median of 131 months. 11 of these patients showed stable or regressive AE lesions as determined by volumetric measurement. One patient developed progressive lesions with persistently negative anti-Em18 antibody ELISA but slight FDG-uptake in repeated PET imaging. At baseline, patients who met criteria for treatment cessation demonstrated higher lymphocyte count and lower total IgE. CONCLUSION Treatment cessation is feasible in inoperable AE patients, who demonstrate negative anti-Em18 antibody ELISA and PET on follow-up. Close monitoring including sectional imaging is strongly advised

Bone Regeneration: A Novel Osteoinductive Function of Spongostan by the Interplay between Its Nano- and Microtopography.

Vordemvenne T, Wähnert D, Koettnitz J, et al. Bone Regeneration: A Novel Osteoinductive Function of Spongostan by the Interplay between Its Nano- and Microtopography. Cells. 2020;9(3): 654.Scaffold materials for bone regeneration are crucial for supporting endogenous healing after accidents, infections, or tumor resection. Although beneficial impacts of microtopological or nanotopological cues in scaffold topography are commonly acknowledged, less consideration is given to the interplay between the microscale and nanoscale. Here, micropores with a 60.66 ± 24.48 m diameter ordered by closely packed collagen fibers are identified in pre-wetted Spongostan, a clinically-approved collagen sponge. On a nanoscale level, a corrugated surface of the collagen sponge is observable, leading to the presence of 32.97 ± 1.41 nm pores. This distinct micro- and nanotopography is shown to be solely sufficient for guiding osteogenic differentiation of human stem cells in vitro. Transplantation of Spongostan into a critical-size calvarial rat bone defect further leads to fast regeneration of the lesion. However, masking the micro- and nanotopographical cues using SiO2 nanoparticles prevents bone regeneration in vivo. Therefore, we demonstrate that the identified micropores allow migration of stem cells, which are further driven towards osteogenic differentiation by scaffold nanotopography. The present findings emphasize the necessity of considering both micro- and nanotopographical cues to guide intramembranous ossification, and might provide an optimal cell- and growth-factor-free scaffold for bone regeneration in clinical settings

The Tumor Necrosis Factor Superfamily Members TNFSF14 (LIGHT), Lymphotoxin β and Lymphotoxin β Receptor Interact to Regulate Intestinal Inflammation

Over 1.5 million individuals in the United States are afflicted with inflammatory bowel disease (IBD). While the progression of IBD is multifactorial, chronic, unresolved inflammation certainly plays a key role. Additionally, while multiple immune mediators have been shown to affect pathogenesis, a comprehensive understanding of disease progression is lacking. Previous work has demonstrated that a member of the TNF superfamily, TNFSF14 (LIGHT), which is pro-inflammatory in several contexts, surprisingly plays an important role in protection from inflammation in mouse models of colitis, with LIGHT deficient mice having more severe disease pathogenesis. However, LIGHT is a single member of a complex signaling network. It signals through multiple receptors, including herpes virus entry mediator (HVEM) and lymphotoxin beta receptor (LTβR); these two receptors in turn can bind to other ligands. It remains unknown which receptors and competing ligands can mediate or counteract the outcome of LIGHT-signaling during colitis. Here we demonstrate that LIGHT signaling through LTβR, rather than HVEM, plays a critical role in the progression of DSS-induced colitis, as LTβR deficient mice exhibit a more severe disease phenotype. Further, mice deficient in LTαβ do not exhibit differential colitis progression compared to WT mice. However, deletion of both LIGHT and LTαβ, but not deletion of both LTαβ and LTβR, resulted in a reversal of the adverse effects associated with the loss of LIGHT. In sum, the LIGHT/LTαβ/LTβR signaling network contributes to DSS colitis, but there may be additional receptors or indirect effects, and therefore, the relationships between these receptors and ligands remains enigmatic

The hierarchical build-up of the Tully-Fisher relation

We use the semi-analytic model GalICS to predict the Tully-Fisher relation in the B, I and for the first time, in the K band, and its evolution with redshift, up to z~1. We refined the determination of the disk galaxies rotation velocity, with a dynamical recipe for the rotation curve, rather than a simple conversion from the total mass to maximum velocity. The new recipe takes into account the disk shape factor, and the angular momentum transfer occurring during secular evolution leading to the formation of bulges. This produces model rotation velocities that are lower by ~20-25% for the majority of the spirals. We implemented stellar population models with a complete treatment of the TP-AGB, which leads to a revision of the mass-to-light ratio in the near-IR. I/K band luminosities increase by ~0.3/0.5 mags at redshift z=0 and by ~0.5/1 mags at z=3. With these two new recipes in place, the comparison between the predicted Tully-Fisher relation with a series of datasets in the optical and near-IR, at redshifts between 0 and 1, is used as a diagnostics of the assembly and evolution of spiral galaxies in the model. At 0.4<z<1.2 the match between the new model and data is remarkably good, especially for later-type spirals (Sb/Sc). At z=0 the new model shows a net improvement in comparison with its original version of 2003, and in accord with recent observations in the K band, the model Tully-Fisher also shows a morphological differentiation. However, in all bands the z=0 model Tully-Fisher is too bright. We argue that this behaviour is caused by inadequate star formation histories in the model galaxies at low redshifts. The star-formation rate declines too slowly, due to continuous gas infall that is not efficiently suppressed. An analysis of the model disk scale lengths, at odds with observations, hints to some missing physics in the modeling of disk formation inside dark matter halos.Comment: Accepted for publication on MNRAS. 2 new plots, 1 new section, and extended discussion. 21 pages, 11 figures in tota