743 research outputs found
Relativistic Charged Spheres II: Regularity and Stability
We present new results concerning the existence of static, electrically
charged, perfect fluid spheres that have a regular interior and are arbitrarily
close to a maximally charged black-hole state. These configurations are
described by exact solutions of Einstein's field equations. A family of these
solutions had already be found (de Felice et al., 1995) but here we generalize
that result to cases with different charge distribution within the spheres and
show, in an appropriate parameter space, that the set of such physically
reasonable solutions has a non zero measure. We also perform a perturbation
analysis and identify the solutions which are stable against adiabatic radial
perturbations. We then suggest that the stable configurations can be considered
as classic models of charged particles. Finally our results are used to show
that a conjecture of Kristiansson et al. (1998) is incorrect.Comment: revtex, 13 pages. five EPS figures. Accepted by CQ
Gravitational lensing in spherically symmetric static spacetimes with centrifugal force reversal
In Schwarzschild spacetime the value of the radius coordinate is
characterized by three different properties: (a) there is a ``light sphere'',
(b) there is ``centrifugal force reversal'', (c) it is the upper limiting
radius for a non-transparent Schwarschild source to act as a gravitational lens
that produces infinitely many images. In this paper we prove a theorem to the
effect that these three properties are intimately related in {\em any}
spherically symmetric static spacetime. We illustrate the general results with
some examples including black-hole spacetimes and Morris-Thorne wormholes.Comment: 18 pages, 3 eps-figure
Incorporating molecular data in fungal systematics: a guide for aspiring researchers
The last twenty years have witnessed molecular data emerge as a primary
research instrument in most branches of mycology. Fungal systematics, taxonomy,
and ecology have all seen tremendous progress and have undergone rapid,
far-reaching changes as disciplines in the wake of continual improvement in DNA
sequencing technology. A taxonomic study that draws from molecular data
involves a long series of steps, ranging from taxon sampling through the
various laboratory procedures and data analysis to the publication process. All
steps are important and influence the results and the way they are perceived by
the scientific community. The present paper provides a reflective overview of
all major steps in such a project with the purpose to assist research students
about to begin their first study using DNA-based methods. We also take the
opportunity to discuss the role of taxonomy in biology and the life sciences in
general in the light of molecular data. While the best way to learn molecular
methods is to work side by side with someone experienced, we hope that the
present paper will serve to lower the learning threshold for the reader.Comment: Submitted to Current Research in Environmental and Applied Mycology -
comments most welcom
Quinolone resistance mutations in the faecal microbiota of Swedish travellers to India
Background: International travel contributes to the spread of antibiotic resistant bacteria over the world. Most studies addressing travel-related changes in the faecal flora have focused on specific mobile resistance genes, or depended on culturing of individual bacterial isolates. Antibiotic resistance can, however, also spread via travellers colonized by bacteria carrying chromosomal antibiotic resistance mutations, but this has received little attention so far. Here we aimed at exploring the abundance of chromosomal quinolone resistance mutations in Escherichia communities residing in the gut of Swedish travellers, and to determine potential changes after visiting India. Sweden is a country with a comparably low degree of quinolone use and quinolone resistance, whereas the opposite is true for India. Methods: Massively parallel amplicon sequencing targeting the quinolone-resistance determining region of gyrA and parC was applied to total DNA extracted from faecal samples. Paired samples were collected from 12 Swedish medical students before and after a 4-15 week visit to India. Twelve Indian residents were included for additional comparisons. Methods known resistance mutations were common in Swedes before travel as well as in Indians, with a trend for all mutations to be more common in the Indian sub group. There was a significant increase in the abundance of the most common amino acid substitution in GyrA (S83L, from 44 to 72 %, p = 0.036) in the samples collected after return to Sweden. No other substitution, including others commonly associated with quinolone resistance (D87N in GyrA, S80I in ParC) changed significantly. The number of distinct genotypes encoded in each traveller was significantly reduced after their visit to India for both GyrA (p = 0.0020) and ParC (p = 0.0051), indicating a reduced genetic diversity, similar to that found in the Indians. Conclusions: International travel can alter the composition of the Escherichia communities in the faecal flora, favouring bacteria carrying certain resistance mutations, and, thereby, contributes to the global spread of antibiotic resistance. A high abundance of specific mutations in Swedish travellers before visiting India is consistent with the hypothesis that these mutation have no fitness cost even in the absence of an antibiotic selection pressure
Photon capture cones and embedding diagrams of the Ernst spacetime
The differences between the character of the Schwarzschild and Ernst
spacetimes are illustrated by comparing the photon capture cones, and the
embedding diagrams of the sections of the equatorial planes
of both the ordinary and optical reference geometry of these spacetimes. The
non-flat asymptotic character of the Ernst spacetime reflects itself in two
manifest facts: the escape photon cones correspond to purely outward radial
direction, and the embedding diagrams of both the ordinary and optical geometry
shrink to zero radius asymptotically. Using the properties of the embedding
diagrams, regions of these spacetimes which could have similar character are
estimated, and it is argued that they can exist for the Ernst spacetimes with a
sufficiently low strength of the magnetic field.Comment: 12 pages, 7 figure
Structural and biochemical characterization of the environmental MBLs MYO-1, ECV-1 and SHD-1
BACKGROUND: MBLs form a large and heterogeneous group of bacterial enzymes conferring resistance to β-lactam antibiotics, including carbapenems. A large environmental reservoir of MBLs has been identified, which can act as a source for transfer into human pathogens. Therefore, structural investigation of environmental and clinically rare MBLs can give new insights into structure-activity relationships to explore the role of catalytic and second shell residues, which are under selective pressure. OBJECTIVES: To investigate the structure and activity of the environmental subclass B1 MBLs MYO-1, SHD-1 and ECV-1. METHODS: The respective genes of these MBLs were cloned into vectors and expressed in Escherichia coli. Purified enzymes were characterized with respect to their catalytic efficiency (kcat/Km). The enzymatic activities and MICs were determined for a panel of different β-lactams, including penicillins, cephalosporins and carbapenems. Thermostability was measured and structures were solved using X-ray crystallography (MYO-1 and ECV-1) or generated by homology modelling (SHD-1). RESULTS: Expression of the environmental MBLs in E. coli resulted in the characteristic MBL profile, not affecting aztreonam susceptibility and decreasing susceptibility to carbapenems, cephalosporins and penicillins. The purified enzymes showed variable catalytic activity in the order of <5% to ∼70% compared with the clinically widespread NDM-1. The thermostability of ECV-1 and SHD-1 was up to 8\ub0C higher than that of MYO-1 and NDM-1. Using solved structures and molecular modelling, we identified differences in their second shell composition, possibly responsible for their relatively low hydrolytic activity. CONCLUSIONS: These results show the importance of environmental species acting as reservoirs for MBL-encoding genes
Causal structure of acoustic spacetimes
The so-called ``analogue models of general relativity'' provide a number of
specific physical systems, well outside the traditional realm of general
relativity, that nevertheless are well-described by the differential geometry
of curved spacetime. Specifically, the propagation of acoustic disturbances in
moving fluids are described by ``effective metrics'' that carry with them
notions of ``causal structure'' as determined by an exchange of sound signals.
These acoustic causal structures serve as specific examples of what can be done
in the presence of a Lorentzian metric without having recourse to the Einstein
equations of general relativity. (After all, the underlying fluid mechanics is
governed by the equations of traditional hydrodynamics, not by the Einstein
equations.) In this article we take a careful look at what can be said about
the causal structure of acoustic spacetimes, focusing on those containing sonic
points or horizons, both with a view to seeing what is different from standard
general relativity, and to seeing what the similarities might be.Comment: 51 pages, 39 figures (23 colour figures, colour used to convey
physics information.) V2: Two references added, some additional discussion of
maximal analytic extension, plus minor cosmetic change
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