Avalia??o dos efeitos do tratamento com ?cidos graxos de cadeia curta sobre a infec??o pelo v?rus sincicial respirat?rio

Submitted by PPG Pediatria e Sa?de da Crian?a ([email protected]) on 2018-07-11T11:29:52Z No. of bitstreams: 1 Disserta??o Krist1107.pdf: 2340518 bytes, checksum: c4a07ad9b25622d62d44660937fb1ea0 (MD5)Approved for entry into archive by Sheila Dias ([email protected]) on 2018-07-16T11:22:39Z (GMT) No. of bitstreams: 1 Disserta??o Krist1107.pdf: 2340518 bytes, checksum: c4a07ad9b25622d62d44660937fb1ea0 (MD5)Made available in DSpace on 2018-07-16T11:30:20Z (GMT). No. of bitstreams: 1 Disserta??o Krist1107.pdf: 2340518 bytes, checksum: c4a07ad9b25622d62d44660937fb1ea0 (MD5) Previous issue date: 2017-03-03Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPE

Effect of LPS on the Viability and Proliferation of Human Oral and Esophageal Cancer Cell Lines

The esophagus and mouth tumors are very frequent malignancies worldwide. Lipopolysaccharides (LPS) are capable of regulating gene expression of pro-inflammatory cytokines by binding to toll-like receptor 4 (TLR4). Recent studies show that LPS can increase the migration ability of human esophageal cancer cell line HKESC-2 by increasing its adhesion properties. However, the effect of LPS has not been tested on viability of human esophageal and oral cancer cells. This study aimed to determine the action of LPS on the cell proliferation and viability in OE19 (adenocarcinoma) and OE21 (squamous carcinoma) cell lines, representative of human esophageal cancer, and HN30 cell line, representative of human oral carcinoma. LPS was used as treatment to OE19 and OE21 cells, and PgLPS (Porphyromonasgingivalis lipopolysaccharide) to HN30 cells. Viability was assessed by MTT assay and proliferation by cell counting. TLR4 expression was evaluated by real-time PCR. LPS at higher concentrations decreased significantly cell viability in both cell lines, adenocarcinoma (OE19) and squamous esophageal carcinoma (OE21) at different times of treatment. In addition, both cell lines, OE19 and OE21, expressed TLR4 receptor. Taken together, our data demonstrated that LPS at high concentrations might contribute to tumor death, in agreement with previously data

Microbiota-derived acetate protects against respiratory syncytial virus infection through a GPR43-type 1 interferon response

Severe respiratory syncytial virus (RSV) infection is a major cause of morbidity and mortality in infants <2 years-old. Here we describe that high-fiber diet protects mice from RSV infection. This effect was dependent on intestinal microbiota and production of acetate. Oral administration of acetate mediated interferon-beta (IFN-beta) response by increasing expression of interferon-stimulated genes in the lung. These effects were associated with reduction of viral load and pulmonary inflammation in RSV-infected mice. Type 1 IFN signaling via the IFN-1 receptor (IFNAR) was essential for acetate antiviral activity in pulmonary epithelial cell lines and for the acetate protective effect in RSV-infected mice. Activation of Gpr43 in pulmonary epithelial cells reduced virus-induced cytotoxicity and promoted antiviral effects through IFN-beta response. The effect of acetate on RSV infection was abolished in Gpr43(-/-) mice. Our findings reveal antiviral effects of acetate involving IFN-beta in lung epithelial cells and engagement of GPR43 and IFNAR10CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES036270/2016sem informação17/2551-0001 380-