Hydrolysis of tannic acid catalyzed by immobilized-stabilized derivatives of tannase from Lactobacillus plantarum

A recombinant tannase from Lactobacillus plantarum, overexpressed in Escherichia coli, was purified in a single step by metal chelate affinity chromatography on poorly activated nickel supports. It was possible to obtain 0.9 g of a pure enzyme by using only 20 mL of chromatographic support. The pure enzyme was immobilized and stabilized by multipoint covalent immobilization on highly activated glyoxyl agarose. Derivatives obtained by multipoint and multisubunit immobilization were 500- and 1000-fold more stable than both the soluble enzyme and the one-point-immobilized enzyme in experiments of thermal and cosolvent inactivation, respectively. In addition, up to 70 mg of pure enzyme was immobilized on 1 g of wet support. The hydrolysis of tannic acid was optimized by using the new immobilized tannase derivative. The optimal reaction conditions were 30% diglyme at pH 5.0 and 4 C. Under these conditions, it was possible to obtain 47.5 mM gallic acid from 5 mM tannic acid as substrate. The product was pure as proved by HPLC. On the other hand, the immobilized biocatalyst preserved >95% of its initial activity after 1 month of incubation under the optimal reaction conditionsThis work was supported by Grants AGL2008-01052, AGL-2009-07625, Consolider INGENIO 2010 CSD2007-00063 FUN-C-FOOD(CICYT),RM2008-00002 (INIA), and S-0505/AGR/000153 (CAM). J.A.C. is the recipient of a predoctoral fellowship from the I3P-CSIC Program and FPI-MEC, and G.F.-L. and L.B. are recipients of Ramon y Cajal postdoctoral contracts.Peer reviewe

Биогеохимические исследования урбанизированных ландшафтов восточной Финляндии

Объектом исследования являются урбанизированные ландшафты трех городов Восточной Финляндии г. Юука, г. Оутокумпу, г. Миккели. Цель работы - биогеохимические исследования урбанизированных ландшафтов восточной Финляндии по результатам исследования проб почв, листьев и стеблей черники, лишайника, листьев древесных растений. В процессе исследования проводились обзор и анализ ранее проведенных работ, анализы литературных, нормативных и фондовых источников, анализировались статистических данные и элементный состав комплекса природных сред. В результате исследования была изучена геоэкологическая обстановка урбанизированных территорий Восточной Финляндии Степень внедрения результатов: полученный анализ эколого-геохимической ситуации урбанизированных территорий Финляндии является составной частью комплексного эколого-геохимического мониторинга территорий, проводимого кафедрой ГЭГХ ТПУ. Аналитический материал будет использован в учебном процессе. Область применения: результаты эколого-геохимического мониторинга урбанизированных территорий Финляндии могут быть использованы природоохранными организациями для ориентировочных данных по содержанию химических элементов в почвах и живых объектах городских агломераций.Object of research are urban landscapes of the three cities in the Eastern Finland city of Juuka, in Outokumpu, the city of Mikkeli. Purpose - biogeochemical studies of the urban landscape of Eastern Finland the results of the research of samples of soil, leaves and stems of bilberry, lichen, leaves of woody plants. In the process of investigation the review and analysis of earlier conducted works, literary analyses, regulatory and archival sources, analyzed statistical data and elemental composition of complex natural environments. The study was studied geo-ecological situation in the urbanized territories of Eastern Finland level of implementation of results: the analysis of environmental and geochemical situation in the urbanized territories of Finland is part of an integrated ecological-geochemical monitoring of the territories held by the Department GEGH TPU. Analytical material will be used in the educational process. Application field: the results of ecological-geochemical monitoring in the urbanized territories of Finland can be used by conservation organizations for estimates on the content of chemical elements in the soils and the living facilities of urban agglomerations

Challenging the field: Bourdieu and men's health

©2014 Macmillan Publishers Ltd. This article considers how understandings of health promotion with men may be assisted by engagement with Bourdieu's theoretical work. The article outlines leading concepts within Bourdieu's work on 'field', 'habitus' and 'capital'; considers subsequent critical debates among gender, feminist and sociological theorists around structure and agency; and links these to discussions within men's health. A particular focus concerns structural disruption of, and movement of social actors between, 'fields' such as family, work and leisure settings. The article examines, through Bourdieu's critical legacy, whether such disruption establishes conditions for transformative reflexivity among men in relation to previously held dispositions (habitus), including those inflected by masculinities, that affect men's health practices. Recent work within Bourdieu's heritage potentially facilitates a re-framing of understandings of men's health practices. The article specifically explores masculine ambivalence within accounts of reflexivity, identities and practice, and considers how social and symbolic (masculine) capital are in play. Implications of Bourdieu's leading concepts for theorising settings-focused approaches to men's health promotion are exemplified with reference to a men's health project in a football stadium leisure setting. The article considers the benefits and challenges of applying gendered critical insights drawing on Bourdieu's work to men's health promotion, and discusses emerging theoretical dilemmas

The BRCA1-Δ11q Alternative Splice Isoform Bypasses Germline Mutations and Promotes Therapeutic Resistance to PARP Inhibition and Cisplatin.

Breast and ovarian cancer patients harboring BRCA1/2 germline mutations have clinically benefitted from therapy with PARP inhibitor (PARPi) or platinum compounds, but acquired resistance limits clinical impact. In this study, we investigated the impact of mutations on BRCA1 isoform expression and therapeutic response. Cancer cell lines and tumors harboring mutations in exon 11 of BRCA1 express a BRCA1-Δ11q splice variant lacking the majority of exon 11. The introduction of frameshift mutations to exon 11 resulted in nonsense-mediated mRNA decay of full-length, but not the BRCA1-Δ11q isoform. CRISPR/Cas9 gene editing as well as overexpression experiments revealed that the BRCA1-Δ11q protein was capable of promoting partial PARPi and cisplatin resistance relative to full-length BRCA1, both in vitro and in vivo Furthermore, spliceosome inhibitors reduced BRCA1-Δ11q levels and sensitized cells carrying exon 11 mutations to PARPi treatment. Taken together, our results provided evidence that cancer cells employ a strategy to remove deleterious germline BRCA1 mutations through alternative mRNA splicing, giving rise to isoforms that retain residual activity and contribute to therapeutic resistance. Cancer Res; 76(9); 2778-90. ©2016 AACR