Analysis of red chalk drawings from the workshop of Giovanni Battista Piranesi using fiber optics reflectance spectroscopy

The viability of fiber optics reflectance spectroscopy (FORS) for the differentiation of red chalk drawing media was investigated, focusing on the group of drawings from the workshop of Giovanni Battista Piranesi (1720–1778) at the Staatliche Kunsthalle Karlsruhe, Germany. The evaluation of spectra was supported by principal component analysis (PCA). The method was tested on mock-up drawings made with recently acquired natural and synthetic red chalks of known origin. It was possible to sort these mock-up drawings according to chalk type and application technique. The compositional differences of these reference chalks were confirmed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Subsequent FORS analysis of selected original drawings revealed the existence of several closely grouped clusters, implying similarities on the basis of the underlying spectral features among the historical red chalks used in Rome. These similarities distinguished the historical drawings from the red chalk mock-up drawings, except for the drawings made with red chalk samples from the area near the town of Theley, Germany, which were shown to bear close similarities to those in the cluster of historical samples

Observation of cirrus clouds with GLORIA during the WISE campaign: detection methods and cirrus characterization

Cirrus clouds contribute to the general radiation budget of the Earth and play an important role in climate projections. Of special interest are optically thin cirrus clouds close to the tropopause due to the fact that their impact is not yet well understood. Measuring these clouds is challenging as both high spatial resolution as well as a very high detection sensitivity are needed. These criteria are fulfilled by the infrared limb sounder GLORIA (Gimballed Limb Observer for Radiance Imaging of the Atmosphere). This study presents a characterization of observed cirrus clouds using the data obtained by GLORIA aboard the German research aircraft HALO during the WISE (Wave-driven ISentropic Exchange) campaign in September and October 2017. We developed an optimized cloud detection method based on the cloud index and the extinction coefficient retrieved at the microwindow 832.4–834.4 cm$^{-1}$. We derived macro-physical characteristics of the detected cirrus clouds such as cloud top height, cloud bottom height, vertical extent and cloud top position with respect to the tropopause. The fraction of cirrus clouds detected above the tropopause is on the order of 13 % to 27 %. In general, good agreement with the clouds predicted by the ERA5 reanalysis dataset is obtained. However, cloud occurrence is ≈ 50 % higher in the observations for the region close to and above the tropopause. Cloud bottom heights are also detected above the tropopause. However, considering the uncertainties, we cannot confirm the formation of unattached cirrus layers above the tropopause

Cirrus cloud shape detection by tomographic extinction retrievals from infrared limb emission sounder measurements

An improved cloud-index-based method for the detection of clouds in limb sounder data is presented that exploits the spatial overlap of measurements to more precisely detect the location of (optically thin) clouds. A second method based on a tomographic extinction retrieval is also presented. Using CALIPSO data and a generic advanced infrared limb imaging instrument as examples for a synthetic study, the new cloud index method has a better horizontal resolution in comparison to the traditional cloud index and has a reduction of false positive cloud detection events by about 30 %. The results for the extinction retrieval even show an improvement of 60 %. In a second step, the extinction retrieval is applied to real 3-D measurements of the airborne Gimballed Limb Observer for Radiance Imaging in the Atmosphere (GLORIA) taken during the Wave-driven ISentropic Exchange (WISE) campaign to retrieve small-scale cirrus clouds with high spatial accuracy

Soft-gluon resummation for squark and gluino hadroproduction

We consider the resummation of soft gluon emission for squark and gluino hadroproduction at next-to-leading-logarithmic (NLL) accuracy in the framework of the minimal supersymmetric standard model. We present analytical results for squark-squark and squark-gluino production and provide numerical predictions for all squark and gluino pair-production processes at the Tevatron and at the LHC. The size of the soft-gluon corrections and the reduction in the scale uncertainty are most significant for processes involving gluino production. At the LHC, where the sensitivity to squark and gluino masses ranges up to 3 TeV, the corrections due to NLL resummation over and above the NLO predictions can be as high as 35% in the case of gluino-pair production, whereas at the Tevatron, the NLL corrections are close to 40% for squark-gluino final states with sparticle masses around 500 GeV.Comment: 31 pages, 7 figure

Supersymmetric top and bottom squark production at hadron colliders

The scalar partners of top and bottom quarks are expected to be the lightest squarks in supersymmetric theories, with potentially large cross sections at hadron colliders. We present predictions for the production of top and bottom squarks at the Tevatron and the LHC, including next-to-leading order corrections in supersymmetric QCD and the resummation of soft gluon emission at next-to-leading-logarithmic accuracy. We discuss the impact of the higher-order corrections on total cross sections and transverse-momentum distributions, and provide an estimate of the theoretical uncertainty due to scale variation and the parton distribution functions.Comment: 29 pages, 6 figure

Measles transmission from an anthroposophic community to the general population, Germany 2008

<p>Abstract</p> <p>Background</p> <p>In Germany, measles vaccination coverage with two doses is not yet sufficient to prevent regional outbreaks. Among the 16 German federal states, vaccination coverage was lowest in Bavaria with 85% in 2008. From March to mid-April 2008, four neighbouring Bavarian counties reported 55 measles-cases mostly linked to an ongoing measles outbreak in an anthroposophic school in Austria. We investigated this outbreak to guide future public health action.</p> <p>Methods</p> <p>We applied the German national case-definition for measles and collected data using the national surveillance system and a questionnaire. Measles cases with disease onset a maximum of 18 days apart and spatial contact (e.g. same household, same school) were summed up in clusters. Two different interventions, which were implemented in schools and kindergartens in Bavaria, were compared by their impact on the size and duration of measles clusters. Susceptible persons were excluded from schools or kindergartens either with the first (intervention A) or second (intervention B) measles case occurring in the respective institution.</p> <p>Results</p> <p>Among the 217 Bavarian measles cases identified from March-July 2008, 28 (13%) cases were attendees of the anthroposophic school in Austria. In total, vaccination status was known in 161 (74%) cases and 156 (97%) of them were not vaccinated. The main factor for non-vaccination was "fear of vaccine-related adverse events" (33%). Twenty-nine (18%) of 161 cases suffered complications. Exclusively genotype D5 was detected. Overall, 184 cases could be epidemiologically grouped into 59 clusters. Of those, 41 clusters could be linked to households and 13 to schools or kindergartens. The effect of intervention A and B was analysed in 10 school or kindergarten clusters. Depending on the respective intervention A or B, the median number of cases per cluster was 3 versus 13 (p = 0.05), and the median duration of a cluster was 3 versus 26 days (p = 0.13).</p> <p>Conclusions</p> <p>Introduction of measles virus into a pocket of susceptible persons (e.g. vaccination opponents or sceptics) may lead to large outbreaks in the general population, if the general population's vaccination coverage is below the WHO recommended level. Education on the safety of measles vaccine needs to be strengthened to increase measles vaccination coverage. Early intervention may limit spread in schools or kindergartens. Suspected measles has to be reported immediately to the local health authorities in order to allow intervention as early as possible.</p

Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells

High attrition rates of novel anti-cancer drugs highlight the need for improved models to predict toxicity. Although polo-like kinase 1 (Plk1) inhibitors are attractive candidates for drug development, the role of Plk1 in primary cells remains widely unexplored. Therefore, we evaluated the utility of an RNA interference-based model to assess responses to an inducible knockdown (iKD) of Plk1 in adult mice. Here we show that Plk1 silencing can be achieved in several organs, although adverse events are rare. We compared responses in Plk1-iKD mice with those in primary cells kept under controlled culture conditions. In contrast to the addiction of many cancer cell lines to the non-oncogene Plk1, the primary cells' proliferation, spindle assembly and apoptosis exhibit only a low dependency on Plk1. Responses to Plk1-depletion, both in cultured primary cells and in our iKD-mouse model, correspond well and thus provide the basis for using validated iKD mice in predicting responses to therapeutic interventions

Contralateral breast cancer risk in patients with ductal carcinoma in situ and invasive breast cancer

We aimed to assess contralateral breast cancer (CBC) risk in patients with ductal carcinoma in situ (DCIS) compared with invasive breast cancer (BC). Women diagnosed with DCIS (N = 28,003) or stage I–III BC (N = 275,836) between 1989 and 2017 were identified from the nationwide Netherlands Cancer Registry. Cumulative incidences were estimated, accounting for competing risks, and hazard ratios (HRs) for metachronous invasive CBC. To evaluate effects of adjuvant systemic therapy and screening, separate analyses were performed for stage I BC without adjuvant systemic therapy and by mode of first BC detection. Multivariable models including clinico-pathological and treatment data were created to assess CBC risk prediction performance in DCIS patients. The 10- year cumulative incidence of invasive CBC was 4.8% for DCIS patients (CBC = 1334). Invasive CBC risk was higher in DCIS patients compared with invasive BC overall (HR = 1.10, 95% confidence interval (CI) = 1.04–1.17), and lower compared with stage I BC without adjuvant systemic therapy (HR = 0.87; 95% CI = 0.82–0.92). In patients diagnosed ≥2011, the HR for invasive CBC was 1.38 (95% CI = 1.35–1.68) after screen-detected DCIS compared with screen-detected invasive BC, and was 2.14 (95% CI = 1.46–3.13) when not screen-detected. The C-index was 0.52 (95% CI = 0.50–0.54) for invasive CBC prediction in DCIS patients. In conclusion, CBC risks are low overall. DCIS patients had a slightly higher risk of invasive CBC compared with invasive BC, likely explained by the risk-reducing effect of (neo)adjuvant systemic therapy among BC patients. For support of clinical decision making more information is needed to differentiate CBC risks among DCIS patient

Biological properties of extracellular vesicles and their physiological functions

In the past decade, extracellular vesicles (EVs) have been recognized as potent vehicles of intercellular communication, both in prokaryotes and eukaryotes. This is due to their capacity to transfer proteins, lipids and nucleic acids, thereby influencing various physiological and pathological functions of both recipient and parent cells. While intensive investigation has targeted the role of EVs in different pathological processes, for example, in cancer and autoimmune diseases, the EV-mediated maintenance of homeostasis and the regulation of physiological functions have remained less explored. Here, we provide a comprehensive overview of the current understanding of the physiological roles of EVs, which has been written by crowd-sourcing, drawing on the unique EV expertise of academia-based scientists, clinicians and industry based in 27 European countries, the United States and Australia. This review is intended to be of relevance to both researchers already working on EV biology and to newcomers who will encounter this universal cell biological system. Therefore, here we address the molecular contents and functions of EVs in various tissues and body fluids from cell systems to organs. We also review the physiological mechanisms of EVs in bacteria, lower eukaryotes and plants to highlight the functional uniformity of this emerging communication system