Cold Climate Winegrape Cultivar Sensitivity to Sulfur in the Northern Great Plains Region of the United States

Elemental sulfur is commonly applied for powdery mildew (Erysiphe necator) protection on winegrape (Vitis sp.). The product may be used in a diversified, integrated disease management system to help prevent fungicide resistance to products with other modes of action. Additionally, sulfur may be used as a control option in organic systems. Applications of sulfur have been known to cause phytotoxic injury to susceptible winegrape cultivars, particularly those stemming from fox grape (Vitis labrusca) parentage. To improve recommendations to producers in the northern Great Plains region of the United States, a comparison of injury incidence and severity, as well as effects on yield characteristics was undertaken for 13 regional cultivars exposed to three sulfur rates (0, 2.4, and 4.8 lb/acre a.i.) at a North Dakota State University Research Station near Absaraka, ND. Overall, four cultivars (Bluebell, Baltica, Sabrevois, and King of the North) of the 13 cultivars tested showed phytotoxic symptoms. Injury severity and incidence of these cultivars differed between years and across rates. 'Bluebell' showed consistent and severe sulfur injury symptoms. Injury to the other three susceptible cultivars tended to vary by the given environment, with King of the North generally showing the lowest injury response. Injury symptoms were not found to be associated with the overall yield or cluster weight. Results suggest that alternative spray programs that exclude sulfur- based fungicides should be recommended for ' Bluebell', 'Baltica', 'Sabrevois', and 'King of the North', whereas sulfur- based fungicides may be applied to 'Alpenglow', 'ES 12- 6- 18', 'Frontenac', 'Frontenac Gris', 'La Crescent', 'Marquette', 'Somerset Seedless', 'St. Croix', and 'Valiant'. Observations on fruit ripening in 2014 suggest that future research is needed to determine if a reduction of fruit quality may occur in some seasons with repeated sulfur applications or with successive annual sulfur applications for susceptible cultivars if used in an organic production system

A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes

Fusarium species, particularly Fusarium graminearum and F culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000-2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F graminearurn, 479 F culmorum, and 3 F cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. grarninearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified.Ministere de l'Agriculture, de la Viticulture et de la Protection des Consommateurs-Administration des Services Techniques de l'Agriculture; M.I.U.R. Project AGROGEN (Laboratory of GENomics for traits of AGROnomic importance in durum wheat: Identification of useful genes, functional analysis and assisted selection by biological markers for the development of the national seed chain) [602/Ric]; Felix Thornley Cobbold Trust; John Oldacre Foundation; Ministry of Agriculture of the Czech RepublicMinistry of Agriculture, Czech Republic [800415]; Spanish Ministry MINECOSpanish Government [AGL201.4-53928-C2-2-R]; Ministry of Agriculture and Food, Norway; Federal Ministry of Education and Research (BMBF) (GABI-KANADA), BonnFederal Ministry of Education & Research (BMBF) [FKZ 0313711A]; German Academic Exchange Service (DAAD), BonnDeutscher Akademischer Austausch Dienst (DAAD) [A/06/92183]; Finnish Ministry of Agriculture and Forestry; Direction Generale de l'Agriculture, Direction de la Recherche [D31-3159, D31-1162, D31-7055]; P.O.R. SARDEGNA F.S.; Danish Directorate for Food, Fisheries and Agri Business [FFS05-3]; Academy of FinlandAcademy of Finland [126917, 131957, 250904, 252162, 267188, 266984]; Olvi Foundation; Turku University Foundation; CIMO travel grant; Nordic network project New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe - Nordic Research Board [090014]The Luxembourg institute of Science and Technology, LU, acknowledges the Ministere de l'Agriculture, de la Viticulture et de la Protection des Consommateurs-Administration des Services Techniques de l'Agriculture for financially supporting the Sentinelle project. The work on Italian strains has been financially supported through the M.I.U.R. Project AGROGEN (Laboratory of GENomics for traits of AGROnomic importance in durum wheat: Identification of useful genes, functional analysis and assisted selection by biological markers for the development of the national seed chain) (D. D. 14.03.2005 n. 602/Ric). Funding for the research of Ryan Basler was provided by Felix Thornley Cobbold Trust and the John Oldacre Foundation.; The work of JC was supported by the Ministry of Agriculture of the Czech Republic, Project No. 800415. The research of MG and PG was supported by the Spanish Ministry MINECO (AGL201.4-53928-C2-2-R). The Ministry of Agriculture and Food, Norway funded the work of IH. The research of TM was funded by the Federal Ministry of Education and Research (BMBF) (GABI-KANADA #FKZ 0313711A), Bonn and by the German Academic Exchange Service (DAAD), Bonn (code no.: A/06/92183). PP acknowledges the Finnish Ministry of Agriculture and Forestry for funding the project FinMyco on Fusarium and mycotoxins in Finland. The research of JS was funded by the Direction Generale de l'Agriculture, Direction de la Recherche (ref. D31-3159, D31-1162, D31-7055), in the framework of a project entitled Caracterization et dynamique des fusarioses sur mais en Region Wallonne. BS acknowledges support by P.O.R. SARDEGNA F.S.E. 2007-2013-Obiettivo competitivita regionale e occupazione, Asse IV Capitale umano, Linea di Attivita 1.3.1 (research project Identification of natural and natural-like molecules inhibiting mycotoxin biosynthesis by Fusaria pathogenic on cereals). UT thanks the Danish Directorate for Food, Fisheries and Agri Business grant FFS05-3 for financial support. The work of TY was financially supported by the Academy of Finland (no. 126917, 131957, 250904, 252162, 267188, and 266984), Olvi Foundation, Turku University Foundation, a CIMO travel grant to Taha Hussien, and the Nordic network project New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe (project 090014), which was funded by the Nordic Research Board

Behavioural risk factors in two generations of non-Western migrants: do trends converge towards the host population?

Migrant mortality does not conform to a single pattern of convergence towards prevalence rates in the host population. To understand better how migrant mortality develops, it is necessary to further investigate how the underlying behavioural determinants change following migration. We studied whether the prevalence of behavioural risk factors over two generations of Turkish and Moroccan migrants converge towards the prevalence rates in the Dutch population. From a random sample from the population register of Amsterdam, 291 Moroccan and 505 Turkish migrants, aged 15–30, participated in a structured interview that included questions on smoking, alcohol consumption, physical inactivity and weight/height. Data from the Dutch population were available from Statistics Netherlands. By calculating age-adjusted Odds Ratio’s, prevalence rates among both generations were compared with prevalence rates in the host population for men and women separately. We found indications of convergence across generations towards the prevalence rates in the host population for smoking in Turkish men, for overweight in Turkish and Moroccan women and for physical inactivity in Turkish women. Alcohol consumption, however, remained low in all subgroups and did not converge towards the higher rates in the host population. In addition, we found a reversed trend among Turkish women regarding smoking: the second generation smoked significantly more, while the first generation did not differ from ethnic Dutch. In general, behavioural risk factors in two generations of non-Western migrants in the Netherlands seem to converge towards the prevalence rates in the Dutch population. However, some subgroups and risk factors showed a different pattern

A European Database of Fusarium graminearum and F-culmorum Trichothecene Genotypes

Fusarium species, particularly Fusarium graminearum and F culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000-2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F graminearurn, 479 F culmorum, and 3 F cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. grarninearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified

A Rare Tumor in the Cervical Sympathetic Trunk: Ganglioneuroblastoma

Ganglioneuroblastoma is a rare tumor with moderate malignancy, which is composed of mature ganglion cells and seen in sympathetic ganglia and adrenal medulla. The diagnosis is possible after cytological and immunohistochemical studies following a needle biopsy or surgical excision. There is no consensus regarding the need for chemo-or radiotherapy after surgery. In this case report, clinical behavior and diagnosis and treatment of the rare tumor cervical ganglioneuroblastoma were discussed

What preconceptions and attitudes about engineering are prevalent amongst upper secondary school pupils? An international study

n the Netherlands, as well as in many other countries, there is an increasing interest in implementing education about engineering as a part of general education at the upper secondary school level. In order to know what pupils at that level think about engineering, a study has been done to investigate their attitude towards and their concept of engineering. This study was done not only in the Netherlands but also in 39 other countries. In the preparation of the study, pupils were asked to draw concept maps of what they thought engineering was about. In the quantitative main part of the study, pupils were asked to respond to 33 Likert-type attitude items and 32 concept-items of the same type. A factor analysis was done in order to reveal the dimensions in the pupils’ attitude and concept. In contrast to studies carried out amongst students in lower secondary school levels, a fairly clear concept about engineering was found. The gender differences relating to engineering, found in lower secondary education, were not found in upper secondary education. The prevalent attitude of students towards engineering indicated a fairly positive image of engineering.Applied Science

Analysis of Polygalacturonase Enzyme Production in Isolates of Botrytis cinerea Sensitive to Different Fungicides Used in Vineyards

WOS: 000283729600006Reduction of mycelial growth and spore germination of Botrytis cinerea, the causal agent of grey mould, are common criteria to determine the sensitivity of this fungus to fungicides. In this article, the influence of nine different fungicides on polygalacturonase (PG) activity and mycelial dry weight (MDW) was analysed on culture filtrates from B. cinerea, obtained from grapes. All fungicides except triadimenol and tebuconazole inhibited MDW of isolates <50%. Cyprodinil + fludioxonil, myclobutanil and imazalil inhibited PG activity more than 50%. Fenhexamid had a lower inhibitory effect (<50%) on PG activity. Procymidone and pyrimethanil induced both PG activity and isoenzyme banding profile of isolates sensitive to these fungicides. This study provides a new additional tool for determining sensitivity to fungicides and monitoring the effect of fungicide resistance management policies.Trakya UniversityTrakya University [TUBAP-664]This research was supported by the Scientific Research Fund of Trakya University (TUBAP-664). We are grateful to Dr Martha Rowe (University of Nebraska-Lincoln) for improving the language and for useful remarks

Three-dimensional finite element analysis of stress distribution in inlay-restored mandibular first molar under simultaneous thermomechanical loads

Functional occlusal loads and intraoral temperature changes create stress in teeth. The purpose of this study was to evaluate the impact of simultaneous thermomechanical loads on stress distribution related to inlay restored teeth by three-dimensional finite element analysis. A mandibular first molar was constructed with tooth structures, surrounding bone and inlays of Type II gold alloy, ceramic, and composite resin. Stress patterns on the restorative materials, adhesive resin, enamel and dentin were analyzed after simulated temperature changes from 36 degrees C to 4 or 60 degrees C for 2 s with 200-N oblique loading. The results showed that the three types of inlays had similar stress distribution in the tooth structures and restorative materials. Concerning the adhesive resin, the composite resin inlay model exhibited lower stresses than ceramic and gold alloy inlays. Simultaneous thermomechanical loads caused high stress patterns in inlay-restored teeth. Composite resin inlays may be the better choice to avoid adhesive failure

Cold Climate Winegrape Cultivar Sensitivity to Sulfur in the Northern Great Plains Region of the United States

Elemental sulfur is commonly applied for powdery mildew (Erysiphe necator) protection on winegrape (Vitis sp.). The product may be used in a diversified, integrated disease management system to help prevent fungicide resistance to products with other modes of action. Additionally, sulfur may be used as a control option in organic systems. Applications of sulfur have been known to cause phytotoxic injury to susceptible winegrape cultivars, particularly those stemming from fox grape (Vitis labrusca) parentage. To improve recommendations to producers in the northern Great Plains region of the United States, a comparison of injury incidence and severity, as well as effects on yield characteristics was undertaken for 13 regional cultivars exposed to three sulfur rates (0, 2.4, and 4.8 lb/acre a.i.) at a North Dakota State University Research Station near Absaraka, ND. Overall, four cultivars (Bluebell, Baltica, Sabrevois, and King of the North) of the 13 cultivars tested showed phytotoxic symptoms. Injury severity and incidence of these cultivars differed between years and across rates. 'Bluebell' showed consistent and severe sulfur injury symptoms. Injury to the other three susceptible cultivars tended to vary by the given environment, with King of the North generally showing the lowest injury response. Injury symptoms were not found to be associated with the overall yield or cluster weight. Results suggest that alternative spray programs that exclude sulfur- based fungicides should be recommended for ' Bluebell', 'Baltica', 'Sabrevois', and 'King of the North', whereas sulfur- based fungicides may be applied to 'Alpenglow', 'ES 12- 6- 18', 'Frontenac', 'Frontenac Gris', 'La Crescent', 'Marquette', 'Somerset Seedless', 'St. Croix', and 'Valiant'. Observations on fruit ripening in 2014 suggest that future research is needed to determine if a reduction of fruit quality may occur in some seasons with repeated sulfur applications or with successive annual sulfur applications for susceptible cultivars if used in an organic production system