Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model

Bovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It is the most costly disease in the dairy industry because of the high use of antibiotics. Staphylococcus aureus is one of the major causative agents of bovine mastitis and antimicrobial resistance. Therefore, new strategies to control bacterial infection are required in the dairy industry. One potential strategy is bacteriophage (phage) therapy. In the present study, we examined the host range of previously isolated S. aureus phages ΦSA012 and ΦSA039 against S. aureus strains isolated from mastitic cows. These phages could kill all S. aureus (93 strains from 40 genotypes) and methicillin-resistant S. aureus (six strains from six genotypes) strains tested. Using a mouse mastitis model, we demonstrated that ΦSA012 reduced proliferation of S. aureus and inflammation in the mammary gland. Furthermore, intravenous or intraperitoneal phage administration reduced proliferation of S. aureus in the mammary glands. These results suggest that broad host range phages ΦSA012 is potential antibacterial agents for dairy production medicine

Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model

Bovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It is the most costly disease in the dairy industry because of the high use of antibiotics. Staphylococcus aureus is one of the major causative agents of bovine mastitis and antimicrobial resistance. Therefore, new strategies to control bacterial infection are required in the dairy industry. One potential strategy is bacteriophage (phage) therapy. In the present study, we examined the host range of previously isolated S. aureus phages ΦSA012 and ΦSA039 against S. aureus strains isolated from mastitic cows. These phages could kill all S. aureus (93 strains from 40 genotypes) and methicillin-resistant S. aureus (six strains from six genotypes) strains tested. Using a mouse mastitis model, we demonstrated that ΦSA012 reduced proliferation of S. aureus and inflammation in the mammary gland. Furthermore, intravenous or intraperitoneal phage administration reduced proliferation of S. aureus in the mammary glands. These results suggest that broad host range phages ΦSA012 is potential antibacterial agents for dairy production medicine

Spectroscopy of ¹⁷C via one-neutron knockout reaction

21st International Conference on Few-Body Problems in Physics, Chicago, IL, USA, May 18-22, 2015.A spectroscopic study of ¹⁷C was performed via the one-neutron knockout reaction of ¹⁸C on a carbon target at RIKEN-RIBF. Three unbound states at excitation energies of 2.66(2), 3.16(5), and 3.97(3) MeV (preliminary) were observed. The energies are compared with shell-model calculations and existing measurements to deduce their spin-parities. From the comparison, the states at 2.66(2) and 3.97(3) MeV are suggested to be 1/2⁻ and 3/2⁻, respectively. From its decay property, the state at 3.16(5) MeV is indicated to be 9/2⁺

Study of ¹⁹C by One-Neutron Knockout

21st International Conference on Few-Body Problems in Physics, Chicago, IL, USA, May 18-22, 2015.The spectroscopic structure of ¹⁹C, a prominent one-neutron halo nucleus, has been studied with a ²⁰C secondary beam at 290 MeV/nucleon and a carbon target. Neutron-unbound states populated by the one-neutron knockout reaction were investigated by means of the invariant mass method. The preliminary relative energy spectrum and parallel momentum distribution of the knockout residue, ¹⁹C∗, were reconstructed from the measured four momenta of the¹⁸C fragment, neutron, and beam. Three resonances were observed in the spectrum, which correspond to the states at Ex = 0.62(9), 1.42(10), and 2.89(10) MeV. The parallel momentum distributions for the 0.62-MeV and 2.89-MeV states suggest spin-parity assignments of 5/2⁺ and 1/2⁻, respectively. The 1.42-MeV state is in line with the reported 5/22⁺ state

Abscinazole-F1, a conformationally restricted analogue of the plant growth retardant uniconazole and an inhibitor of ABA 8’-hydroxylase CYP707A with no growth-retardant effect

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Spectroscopy of C-17 Above the Neutron Separation Energy

Spectroscopy of an unbound nucleus C-17 was performed using the SAMURAI spectrometer at RIBF of RIKEN. Six resonances were observed for the C-16+n system with relative energies of 0.52, 0.77, 1.36, 1.91, 2.22 and 3.20 MeV. The excitation energies (E-x) of the observed resonances were deduced, by taking into account the states of the C-16 fragments identified by coincident gamma rays, as E-x =(3.02), 1.51, (3.86), 2.65, (4.72) and 3.94MeV. The orbital angular momenta of the two observed states in C-17 at E-x =2.65 and 3.94 MeV were determined as 1 by comparing parallel momentum distributions with theoretical predictions.11Nsciescopu

Study of 19C by One-Neutron Knockout

The spectroscopic structure of 19C, a prominent one-neutron halo nucleus, has been studied with a 20C secondary beam at 290 MeV/nucleon and a carbon target. Neutron-unbound states populated by the one-neutron knockout reaction were investigated by means of the invariant mass method. The preliminary relative energy spectrum and parallel momentum distribution of the knockout residue, 19C*, were reconstructed from the measured four momenta of the 18C fragment, neutron, and beam. Three resonances were observed in the spectrum, which correspond to the states at Ex = 0.62(9), 1.42(10), and 2.89(10) MeV. The parallel momentum distributions for the 0.62-MeV and 2.89-MeV states suggest spin-parity assignments of 5/2+ and 1/2−, respectively. The 1.42-MeV state is in line with the reported 5/22+ state