Three-Dimensional Digital Capture of Head Size in Neonates – A Method Evaluation

Introduction: The quality of neonatal care is mainly determined by long-term neurodevelopmental outcome. The neurodevelopment of preterm infants is related to postnatal head growth and depends on medical interventions such as nutritional support. Head circumference (HC) is currently used as a two-dimensional measure of head growth. Since head deformities are frequently found in preterm infants, HC may not always adequately reflect head growth. Laser aided head shape digitizers offer semiautomatic acquisition of HC and cranial volume (CrV) and could thus be useful in describing head size more precisely. Aims: 1) To evaluate reproducibility of a 3D digital capture system in newborns. 2) To compare manual and digital HC measurements in a neonatal cohort. 3) To determine correlation of HC and CrV and predictive value of HC. Methods: Within a twelve-month period data of head scans with a laser shape digitizer were analysed. Repeated measures were used for method evaluation. Manually and digitally acquired HC was compared. Regression analysis of HC and CrV was performed. Results: Interobserver reliability was excellent for HC (bias-0.005%, 95% Limits of Agreement (LoA) −0.39–0.39%) and CrV (bias1.5%, 95%LoA-0.8–3.6%). Method comparison data was acquired from 282 infants. It revealed interchangeability of the methods (bias-0.45%; 95%LoA-4.55–3.65%) and no significant systematic or proportional differences. HC and CrV correlated (r2 = 0.859, p<0.001), performance of HC predicting CrV was poor (RSD ±24 ml). Correlation was worse in infants with lower postmenstrual age (r2 = 0.745) compared to older infants (r2 = 0.843). Discussion: The current practice of measuring HC for describing head growth in preterm infants could be misleading since it does not represent a 3D approach. CrV can vary substantially in infants of equal HC. The 3D laser scanner represents a new and promising method to provide reproducible data of CrV and HC. Since it does not provide data on cerebral structures, additional imaging is required

Mass spectrometric and quantum mechanical analysis of gas-phase formation, structure, and decomposition of various b2 ions and their specifically deuterated analogs

AbstractB ions represent an important type of fragment ions derived from protonated peptides by cleavage of an amide bond with N-terminal charge retention. Such species have also been discussed as key intermediates during cyclic peptide fragmentation. Detailed structural information on such ion types can facilitate the interpretation of multiple step fragmentations such as the formation of inner chain fragments from linear peptides or the fragmentation of cyclic peptides. The structure of different b2 ion isomers was investigated with collision-induced dissociations (CID) in combination with hydrogen/deuterium (H/D) exchange of the acidic protons. Special care was taken to investigate fragment ions derived from pure gas-phase processes. Structures deduced from the results of the CID analysis were compared with structures predicted on the basis of quantum chemical density functional theory (DFT) calculations to be most stable. The results pointed to different types of structures for b2 ion isomers of complementary amino acid sequences. Either the protonated oxazolone structure or the N-terminally protonated immonium ion structure were proposed on the basis of the CID results and the DFT calculations. In addition, the analysis of different selectively N-alkylated peptide analogs revealed mechanistic details of the processes generating b ions

Measuring Snow Liquid Water Content with Low-Cost GPS Receivers

The amount of liquid water in snow characterizes the wetness of a snowpack. Its temporal evolution plays an important role for wet-snow avalanche prediction, as well as the onset of meltwater release and water availability estimations within a river basin. However, it is still a challenge and a not yet satisfyingly solved issue to measure the liquid water content (LWC) in snow with conventional in situ and remote sensing techniques. We propose a new approach based on the attenuation of microwave radiation in the L-band emitted by the satellites of the Global Positioning System (GPS). For this purpose, we performed a continuous low-cost GPS measurement experiment at the Weissfluhjoch test site in Switzerland, during the snow melt period in 2013. As a measure of signal strength, we analyzed the carrier-to-noise power density ratio (C/N-0) and developed a procedure to normalize these data. The bulk volumetric LWC was determined based on assumptions for attenuation, reflection and refraction of radiation in wet snow. The onset of melt, as well as daily melt-freeze cycles were clearly detected. The temporal evolution of the LWC was closely related to the meteorological and snow-hydrological data. Due to its non-destructive setup, its cost-efficiency and global availability, this approach has the potential to be implemented in distributed sensor networks for avalanche prediction or basin-wide melt onset measurements

How Will Hydroelectric Power Generation Develop under Climate Change Scenarios?

Climate change has a large impact on water resources and thus on hydropower. Hydroelectric power generation is closely linked to the regional hydrological situation of a watershed and reacts sensitively to changes in water quantity and seasonality. The development of hydroelectric power generation in the Upper Danube basin was modelled for two future decades, namely 2021-2030 and 2051-2060, using a special hydropower module coupled with the physically-based hydrological model PROMET. To cover a possible range of uncertainties, 16 climate scenarios were taken as meteorological drivers which were defined from different ensemble outputs of a stochastic climate generator, based on the IPCC-SRES-A1B emission scenario and four regional climate trends. Depending on the trends, the results show a slight to severe decline in hydroelectric power generation. Whilst the mean summer values indicate a decrease, the mean winter values display an increase. To show past and future regional differences within the Upper Danube basin, three hydropower plants at individual locations were selected. Inter-annual differences originate predominately from unequal contributions of the runoff compartments rain, snow-and ice-melt

Climate Change Impacts on the Water Resources in the Danube River Basin and Possibilities to Adapt: – The Way to an Adaptation Strategy and its Update

As the Intergovernmental Panel on Climate Change reported in 2013, climate change will have significant impacts on all water sectors. Since water is essential for live, culture, economy and ecosystems, climate change adaptation is crucial. Therefore, a legal and political framework was established by the commissions of the European Union, the United Nations and on national levels. For the Danube River Basin (DRB), the International Commission for the Protection of the Danube River got the mandate to develop an adaptation strategy in 2012 and to update this strategy in 2018. The natural science basis on which the adaptation strategy and its update are based on are two studies, conducted in 2011/2012 and updated and revised in 2017/18. Numerous documents from actual research and development projects and studies dealing with climate change and its impacts on water related issues were analysed in detail and the results summarised. It is agreed that temperature will increase basin-wide. The precipitation trend shows a strong northwest-southeast gradient and significant changes in seasonality. Runoff patterns will change and extreme weather events will intensify. However, the magnitude of the results shows a strong spatial variability due to the heterogeneity of the DRB., It is assessed that these changes will have mostly negative impacts on all water related sectors. Based on the scientific findings an approach for an improved basin-wide strategy on adaptation to climate change is developed. It includes guiding principles and five categories of adaptation measures targeting different objectives

Synthesis, Characterization, and Biological Properties of Steroidal Ruthenium(II) and Iridium(111) Complexes Based on the Androst-16-en-3-ol Framework

A range of novel cyclometalated ruthenium(II) and iridium(III) complexes with a steroidal backbone based on androsterone were synthesized and characterized by NMR spectroscopy and X-ray crystallography. Their cytotoxic properties in RT112 and RT112 cP (cisplatin-resistant) cell lines as well as in MCF7 and somatic fibroblasts were compared with those of the corresponding nonsteroidal complexes and the noncyclometalated pyridyl complexes as well as with cisplatin as reference. All steroidal complexes were more active in RT112 cP cells than cisplatin, whereby the cyclometalated pyridinylphenyl complexes based on 5c showed high cytotoxicity while maintaining low resistant factors of 0.33 and 0.50.Peer reviewe

Adoptive Cell Transfer of Allogeneic Epstein-Barr Virus-Specific T Lymphocytes for Treatment of Refractory EBV-Associated Posttransplant Smooth Muscle Tumors: A Case Report

Posttransplant smooth muscle tumors (PTSMTs) are rare Epstein-Barr virus (EBV)-associated neoplasms, mostly occurring after solid organ transplantation. Current therapeutic strategies include surgery and reduction of immunosuppressive medication. We describe for the first time a novel treatment approach for PTSMT by adoptive cell transfer (ACT) of EBV-specific T cells to a 20-year-old patient with a medical history of cardiac transplantation, posttransplant lymphoproliferative disease, and multilocular PTSMT. During ACT, mild cytokine release syndrome occurred, while no unexpected safety signals were recorded. We observed in vivo expansion of EBV-specific T cells and reduction of EBV viremia. Best response was stable disease after 4 months with reduction of EBV viremia and normalization of lactate dehydrogenase levels. ACT with EBV-specific T cells may be a safe and efficacious therapeutic option for PTSMT that warrants further exploration