Identification of predation events in wild fish using novel acoustic transmitters

Background: Acoustic telemetry is a commonly used tool to gain knowledge about aquatic animal ecology through the study of their movements. In telemetry studies researchers must make inferences regarding the movements and the fates of tagged animals. Until recently, predation has been inferred in telemetry data using a variety of methods including abrupt changes in movement patterns or habitat use. An acoustic telemetry transmitter has been developed to detect predation events of tagged animals, and while they have performed well in controlled laboratory trials, literature regarding the application of these novel transmitters in field settings is limited. The objective of this research was to describe the detection data obtained from field studies using predation tags and propose methods to incorporate this information in decision-making about the fate of tagged animals. We implanted 60 yellow perch (Perca flavescens) with predation transmitters and evaluated their spatial use in a receiver array (34 ha) using a combination of centres of activity, roaming indices, and step length measures to examine detection data. Results: Over 5 months, 19 apparent predation events were identified by the transmitters. Roaming indices and centres of activity revealed a variety of detection patterns, including instances of altered behaviour before and after predation that matched tag-indentified predation events, dropped tags post-predation, and detections that ceased post-predation indicating the predator might have left the array. Based on the observed patterns, probable predation was inferred for 15 of 19 triggered tags, with unclear fates for four fish. Conclusions: Our study provided a framework to assess the fate of animals tagged with predation transmitters and demonstrate how these tags can contribute to telemetry studies. We showed how detections can be categorized using tag status to compare movement metrics among individuals, provided tools to explore space use surrounding predation events, and synthesized this information to inform uncertainty surrounding tag-identified predation events. Predation tags do not remove all uncertainty about the fate of tagged individuals, but combined with other metrics they increase the likelihood of identifying abnormal movements that could otherwise introduce biased detection histories into studies of small-sized fishes

The influence of dynamic environmental interactions on detection efficiency of acoustic transmitters in a large, deep, freshwater lake

Background: Acoustic telemetry is an increasingly common method used to address ecological questions about the movement, behaviour, and survival of freshwater and marine organisms. The variable performance of acoustic telemetry equipment and ability of receivers to detect signals from transmitters have been well studied in marine and coral reef environments to inform study design and improve data interpretation. Despite the growing use of acoustic telemetry in large, deep, freshwater systems, detection efficiency and range, particularly in relation to environmental variation, are poorly understood. We used an array of 90 69-kHz acoustic receivers and 8 sentinel range transmitters of varying power output deployed at different depths and locations approximately 100-9500 m apart for 215 days to evaluate how the detection efficiency of acoustic receivers varied spatially and temporally in relation to environmental conditions. Results: The maximum distance that tags were detected ranged from 5.9 to 9.3 km. Shallow tags consistently had lower detection efficiency than deep tags of the same power output and detection efficiency declined through the winter months (December-February) of the study. In addition to the distance between tag and receiver, thermocline strength, surface water velocity, ice thickness, water temperature, depth range between tag and receiver, and number of fish detections contributed to explaining variation in detection efficiency throughout the study period. Furthermore, the most significant models incorporated interactions between several environmental variables and tag-receiver distance, demonstrating the complex temporal and spatial relationships that exist in heterogeneous environments. Conclusions: Relying on individual environmental variables in isolation to interpret receiver performance, and thus animal behaviour, may be erroneous when detection efficiency varies across distances, depths, or tag types. As acoustic telemetry becomes more widely used to study ecology and inform management, it is crucial to understand its limitations in heterogeneous environments, such as freshwater lakes, to improve the quality and interpretation of data. We recommend that in situ range testing and retrospective analysis of detection efficiency be incorporated into study design for telemetry projects. Furthermore, we caution against oversimplifying the dynamic relationship between detection efficiency and environmental conditions for the sake of producing a correction that can be applied directly to detection data of tagged animals when the intended correction may not be justified

Global trends in aquatic animal tracking with acoustic telemetry

Acoustic telemetry (AT) is a rapidly evolving technique used to track the movements of aquatic animals. As the capacity of AT research expands it is important to optimize its relevance to management while still pursuing key ecological questions. A global review of AT literature revealed region-specific research priorities underscoring the breadth of how AT is applied, but collectively demonstrated a lack of management-driven objectives, particularly relating to fisheries, climate change, and protection of species. In addition to the need for more research with direct pertinence to management, AT research should prioritize ongoing efforts to create collaborative opportunities, establish long-term and ecosystem-based monitoring, and utilize technological advancements to bolster aquatic policy and ecological understanding worldwide

Long-term retention of acoustic telemetry transmitters in temperate predators revealed by predation tags implanted in wild prey fish

Bloater Coregonus hoyi (n = 48) were implanted with V9DT-2x predation transmitters and monitored on 105 acoustic receivers in eastern Lake Ontario for \u3e6 months. Twenty-three predation events were observed, with predator retention of tags ranging from ≤1 to ≥194 days and 30% of retentions lasting \u3e150 days. Long tag retention times raise concerns for acoustic telemetry analysis and the health of piscivorous predators retaining tags

Effects of Surgically Implanted Acoustic Tags on Body Condition, Growth, and Survival in a Small, Laterally Compressed Forage Fish

Telemetry studies often assume a lack of adverse effects caused by tag attachment and presence in various species and size-classes, which may lead to inaccurate conclusions about fish behavior in field studies. Studies that examine the effects of tagging are typically performed on salmonids and adult fishes rather than on the small fishes that are increasingly becoming the focus of telemetry studies. The objectives of this study were to assess the effects of intracoelomic acoustic tagging on growth, condition, survival, and tag retention in subadult hatchery Bloaters Coregonus hoyi (a focal species for restoration efforts in the Laurentian Great Lakes) and to determine the maximum tag burden below which tag effects are reduced. Fish were either tagged with one of three dummy acoustic transmitters (Vemco V6: n = 50; V7: n = 50; V9: n = 50) or were followed as controls (n = 50; anesthesia, PIT-tagging, and handling only) or sham individuals (n = 49; anesthesia, surgery, suturing, and PIT-tagging but no acoustic tag implanted). Tags represented 1.3–9.0% of body mass. All fish received a PIT tag for individual identification throughout the 6-month monitoring period (November 2014–May 2015). Survival exceeded 90% in all treatment groups, and the tag retention rate was 100%. All surviving fish appeared healthy and in excellent condition at the conclusion of the experiment. The results of this study suggest that acoustic transmitters with a tag mass : body mass ratio of 9% or less can be successfully implanted intracoelomically into subadult Bloaters—small, laterally compressed pelagic fish—with no adverse effects

Performance of acoustic telemetry in relation to submerged aquatic vegetation in a nearshore freshwater habitat

Acoustic telemetry is a powerful tool for learning about the movements and ecology of aquatic animals, but proper use requires evaluation of its performance in different environments. Nearshore freshwater habitats are important to many fishes; however, submerged aquatic vegetation (SAV) in these areas influences the performance of acoustic telemetry through attenuation of the transmissions. Despite this, few studies have quantified the influence of SAV on the detection efficiency and range. We conducted range testing and hydroacoustic surveys to assess the seasonal influence of SAV biovolume on the detection efficiency of 180 kHz transmitters in the nearshore (\u3c1.5 m) habitats of a temperate freshwater riverine ecosystem. The interaction of transmitter-receiver distance and SAV biovolume significantly reduced the detection efficiency of transmitters, which varied with seasonal growth and senescence of SAV. Daily effective detection range (mean ± s.e.) varied from 6.85 m ± 1.98 when SAV coverage was high (mean biovolume 0.98) to 196.08 m ± 51.89 when SAV was largely absent (mean biovolume 0.01). This study demonstrated the impact of SAV on the detection range of acoustic transmitters, illustrating the need for range testing and consideration in study design and analysis to improve the quality of interpretation of data in vegetated habitats

Space use of juvenile and subadult yellow perch (Perca flavescens) in the Detroit River using acoustic telemetry: incorporating variable detection ranges in vegetated areas

Understanding the space use of fishes in early life stages provides information that can contribute to effective fisheries management; however, it can be difficult to track fish in shallow, densely vegetated areas. Using acoustic telemetry, 60 subadult yellow perch (Perca flavescens) were tagged and monitored in a vegetated area of the Detroit River (May-Nov 2018). Variable detection range from submerged aquatic vegetation (SAV) was incorporated in estimates by applying a spatio-temporal correction to aid with interpretation of seasonal changes in activity space. Although subadult yellow perch were commonly detected in the array, demonstrating the importance of SAV habitat (mean detection residency index: 0.85), 60% of individuals were not detected following August, the period with highest activity space estimates, likely due to seasonal movements and predation. Individuals were more commonly detected during the daylight hours compared to night, but activity peaked at crepuscular periods. This study provided spatial information about the often-overlooked early life history of yellow perch, increasing the ecological information available for a species of management and conservation interest in the Great Lakes.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Habitat use and small-scale residence patterns of sympatric sunfish species in a large temperate river

Bluegill (Lepomis macrochirus) and pumpkinseed (Lepomis gibbosus) sunfish function as a trophic link between invertebrates and piscivores in temperate freshwater food webs, but little is known about their movement in large-scale riverine ecosystems. To address this, pumpkinseed and bluegill were implanted with acoustic transmitters and monitored for 5 months (June to November 2015) within a 0.39 km2 acoustic array in the Detroit River. Residence index analysis revealed site fidelity of sunfish to the side of the river they were tagged and a lack of movement across a shipping channel. Bluegill were more active at night and pumpkinseed more active during daylight hours, possibly partitioning resources on a temporal basis, unlike in smaller lakes where the species partition the littoral and pelagic habitats. Pumpkinseed presence was positively correlated with water temperature and level, whereas bluegill presence was not related to any environmental parameters examined. This study demonstrates that anthropogenic alterations (e.g., channelization) influence the movement and distribution of fishes and that fish behaviour in a large river ecosystem can differ from smaller temperate lakes

Application of machine learning to identify predators of stocked fish in Lake Ontario: using acoustic telemetry predation tags to inform management

Understanding predator–prey interactions and food web dynamics is important for ecosystem-based management in aquatic environments, as they experience increasing rates of human-induced changes, such as the addition and removal of fishes. To quantify the post-stocking survival and predation of a prey fish in Lake Ontario, 48 bloater Coregonus hoyi were tagged with acoustic telemetry predation tags and were tracked on an array of 105 acoustic receivers from November 2018 to June 2019. Putative predators of tagged bloater were identified by comparing movement patterns of six species of salmonids (i.e., predators) in Lake Ontario with the post-predated movements of bloater (i.e., prey) using a random forests algorithm, a type of supervised machine learning. A total of 25 bloater (53% of all detected) were consumed by predators on average (± S.D.) 3.1 ± 2.1 days after release. Post-predation detections of predators occurred for an average (± S.D.) of 78.9 ± 76.9 days, providing sufficient detection data to classify movement patterns. Tagged lake trout Salvelinus namaycush provided the most reliable classification from behavioural predictor variables (89% success rate) and was identified as the main consumer of bloater (consumed 50%). Movement networks between predicted and tagged lake trout were significantly correlated over a 6 month period, supporting the classification of lake trout as a common bloater predator. This study demonstrated the ability of supervised learning techniques to provide greater insight into the fate of stocked fishes and predator–prey dynamics, and this technique is widely applicable to inform future stocking and other management efforts

TrackdAT, an acoustic telemetry metadata dataset to support aquatic animal tracking research

Abstract Data on the movement and space use of aquatic animals are crucial to understand complex interactions among biotic and abiotic components of ecosystems and facilitate effective conservation and management. Acoustic telemetry (AT) is a leading method for studying the movement ecology of aquatic animals worldwide, yet the ability to efficiently access study information from AT research is currently lacking, limiting advancements in its application. Here, we describe TrackdAT, an open-source metadata dataset where AT research parameters are catalogued to provide scientists, managers, and other stakeholders with the ability to efficiently identify and evaluate existing peer-reviewed research. Extracted metadata encompasses key information about biological and technical aspects of research, providing a comprehensive summary of existing AT research. TrackdAT currently hosts information from 2,412 journal articles published from 1969 to 2022 spanning 614 species and 380,289 tagged animals. TrackdAT has the potential to enable regional and global mobilization of knowledge, increased opportunities for collaboration, greater stakeholder engagement, and optimization of future ecological research