Nitrogen fixation and isotope composition of native and tame forage plants

Non-Peer ReviewedA plant mixture of alfalfa, purple prairie clover and 7 natives grass species: western wheatgrass, northern wheatgrass, awned wheatgrass, Canada wildrye, green needle grass, little bluestem and blue grama was established at the south farm (SPARC) in Saskatchewan in 2006. Legumes were included in forage stands to sustain pasture production. The fixation of atmospheric derived nitrogen for forage plants was estimated by the method natural abundance of 15N. This method showed that The N2 fixation activity of both alfalfa and purple prairie clover was very important in 2008. They fixed about 90 % of their nitrogen needs. Alfalfa was the good candidate to sustain the production of high quality of forage because it increased the yield of native grass stand by 33 % and increased herbage stand protein by 45 % but this method is failed to detect fixed N in grasses

Stressed out symbiotes:hypotheses for the influence of abiotic stress on arbuscular mycorrhizal fungi

Abiotic stress is a widespread threat to both plant and soil communities. Arbuscular mycorrhizal (AM) fungi can alleviate effects of abiotic stress by improving host plant stress tolerance, but the direct effects of abiotic stress on AM fungi are less well understood. We propose two hypotheses predicting how AM fungi will respond to abiotic stress. The stress exclusion hypothesis predicts that AM fungal abundance and diversity will decrease with persistent abiotic stress. The mycorrhizal stress adaptation hypothesis predicts that AM fungi will evolve in response to abiotic stress to maintain their fitness. We conclude that abiotic stress can have effects on AM fungi independent of the effects on the host plant. AM fungal communities will change in composition in response to abiotic stress, which may mean the loss of important individual species. This could alter feedbacks to the plant community and beyond. AM fungi will adapt to abiotic stress independent of their host plant. The adaptation of AM fungi to abiotic stress should allow the maintenance of the plant-AM fungal mutualism in the face of changing climates. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00442-016-3673-7) contains supplementary material, which is available to authorized users

First assessment of reliability data for the LHC accelerator and detector cryogenic system components

The Large Hadron Collider (LHC) cryogenic system comprises eight independent refrigeration and distribution systems that supply the eight 3.3 km long accelerator sectors with cryogenic refrigeration power as well as four refrigeration systems for the needs of the detectors ATLAS and CMS. In order to ensure the highest possible reliability of the installations, it is important to apply a reliability centred approach for the maintenance. Even though large scale cryogenic refrigeration exists since the mid 20th century, very little third party reliability data is available today. CERN has started to collect data with its computer aided maintenance management system (CAMMS) in 2009, when the accelerator has gone into normal operation. This paper presents the reliability observations from the operation and the maintenance side, as well as statistical data collected by the means of the CAMMS system

Shifts in microbial communities do not explain the response of grassland ecosystem function to plant functional composition and rainfall change

Ecosystem functioning in grasslands is regulated by a range of biotic and abiotic factors, and the role of microbial communities in regulating ecosystem function has been the subject of much recent scrutiny. However, there are still knowledge gaps regarding the impacts of rainfall and vegetation change upon microbial communities and the implications of these changes for ecosystem functioning. We investigated this issue using data from an experimental mesotrophic grassland study in south-east England, which had been subjected to four years of rainfall and plant functional composition manipulations. Soil respiration, nitrogen and phosphorus stocks were measured, and the abundance and community structure of soil microbes were characterised using quantitative PCR and multiplex-TRFLP analysis, respectively. Bacterial community structure was strongly related to the plant functional composition treatments, but not the rainfall treatment. However, there was a strong effect of both rainfall change and plant functional group upon bacterial abundance. There was also a weak interactive effect of the two treatments upon fungal community structure, although fungal abundance was not affected by either treatment. Next, we used a statistical approach to assess whether treatment effects on ecosystem function were regulated by the microbial community. Our results revealed that ecosystem function was influenced by the experimental treatments, but was not related to associated changes to the microbial community. Overall, these results indicate that changes in fungal and bacterial community structure and abundance play a relatively minor role in determining grassland ecosystem function responses to precipitation and plant functional composition change, and that direct effects on soil physical and chemical properties and upon plant and microbial physiology may play a more important role