Choriocarcinoma coexisting with epithelioid trophoblastic tumor of the uterine horn

• We report a choriocarcinoma coexisting with an epithelioid trophoblastic tumor. • Chemotherapy with methotrexate, etoposide, and actinomycin-D was efficacious. • Choriocarcinoma with epithelioid trophoblastic tumor may benefit from chemotherapy

Expression in E. coli of finger-domain lacking tissue-type plasminogen activator with high fibrin affinity

AbstractTissue-type plasminogen activator (t-PA) has a high affinity for fibrin and induces lysis of fibrin (fibrinolysis) on the surface of fibrin without degrading circulating fibrinogen. cDNA for t-PA which lacks the ‘fingerdomain’ (the site for fibrin affinity) was isolated from Detroit 562 cells. Analysis of the nucleotide sequence revealed a lack of the sequences which code for the finger-domain. A plasmid (pDPAT 1) containing the Escherichia coli tac promoter/operator and the cDNA sequence coding for ‘finger-domain lacking t-PA’ was constructed for expression in E. Coli. The polypeptide so produced was a new type of t-PA lacking finger-domain, but revealed plasminogen activator activity with the function of fibrin affinity.Tissue-type plasminogen activatorFinger-domainFibrin affinityPlasmid E. coli tac promoter/operatorDetroit 562 cel

Three-body Coulomb breakup of 11Li in the complex scaling method

Coulomb breakup strengths of 11Li into a three-body 9Li+n+n system are studied in the complex scaling method. We decompose the transition strengths into the contributions from three-body resonances, two-body ``10Li+n'' and three-body ``9Li+n+n'' continuum states. In the calculated results, we cannot find the dipole resonances with a sharp decay width in 11Li. There is a low energy enhancement in the breakup strength, which is produced by both the two- and three-body continuum states. The enhancement given by the three-body continuum states is found to have a strong connection to the halo structure of 11Li. The calculated breakup strength distribution is compared with the experimental data from MSU, RIKEN and GSI.Comment: RevTeX4, 6 pages, 4 figures, Accepted to Phys. Lett. B DOI

Effect of Thiotriazinone Impurity on Insoluble Microparticle Generation Associated with Ceftriaxone-calcium Salt Precipitation in Original (Rocephin®) and Japanese Generic Ceftriaxone Products

This study examined the effect of thiotriazinone impurity on the generation of insoluble microparticles (IMPs) associated with ceftriaxone-calcium salt precipitation in original (Rocephin®) and Japanese generic ceftriaxone (A; Sawai, B; Nichi-Iko) products when mixed with Ca2＋ 4.3mEq/l. We found that the generation rate of IMPs associated with ceftriaxone-calcium salt precipitation among the three ceftriaxone products tested was in the order of generic (A)＜original＜generic (B), as assessed by light obscuration particle counting. Typically, after 60 min, one of the generic ceftriaxone (B)-calcium mixtures was highly opaque with numerous aggregates of milky-white precipitates, the original ceftriaxone-calcium mixture exhibited noticeable IMPs, and the second generic ceftriaxone (A)-calcium mixture was transparent. The levels of thiotriazinone contaminants, known to be a major impurity in ceftriaxone products, were determined by HPLC and found to be in the order of generic A＞original＞generic B. Moreover, the addition of a small amount of thiotriazinone into the generic ceftriaxone (B)-calcium mixture significantly decreased the amount of IMPs, suggesting that the impurity retards ceftriaxone-calcium crystal growth. We thus concluded that the thiotriazinone impurity acts as a suppressive factor of ceftriaxone-calcium salt precipitation, and that the high level of thiotriazinone impurity in the ceftriaxone (B) product could underlie its lowest rate of IMP generation when mixed with calcium. We thus recommend caution regarding the clinical risk of ceftriaxone-calcium compatibility due to impurity contamination in ceftriaxone products

The Japan Public Health Center-based Prospective Study for the Next Generation (JPHC-NEXT): Study Design and Participants

Background: Lifestyle and life-environment factors have undergone drastic changes in Japan over the last few decades. Further, many molecular epidemiologic studies have reported that genetic, epigenetic, and other biomarker information may be useful in predicting individual disease risk.Methods: The Japan Public Health Center-based Prospective Study for the Next Generation (JPHC-NEXT) was launched in 2011 to identify risk factors for lifestyle-related disease, elucidate factors that extend healthy life expectancy, and contribute toward personalized healthcare based on our more than 20 years’ experience with the JPHC Study. From 2011 through 2016, a baseline survey was conducted at 16 municipalities in seven prefectures across the country. A self-administered questionnaire was distributed to all registered residents aged 40–74, which mainly asked about lifestyle factors, such as socio-demographic situation, personal medical history, smoking, alcohol and dietary habits. We obtained informed consent from each participant to participate in this long follow-up study of at least 20 years, including consent to the potential use of their residence registry, medical records, medical fee receipts, care insurance etc., and to the provision of biospecimens (blood and urine), including genomic analysis.Results: As of December 31, 2016, we have established a population-based cohort of 115,385 persons (Response rate 44.1%), among whom 55,278 (47.9% of participants) have provided blood and urine samples. The participation rate was slightly higher among females and in the older age group.Conclusion: We have established a large-scale population-based cohort for next-generation epidemiological study in Japan

Effects of paradoxical sleep deprivation and psychoactive drugs over neural degeneration

A privacao de sono desencadeia uma sindrome com alteracoes fisiologicas bem estabelecidas. Em animais de laboratorio a perda de sono pode, eventualmente, levar a morte. Demonstrou-se que a privacao de sono leva a alteracao na expressao de diversos genes, mecanismos de estresse oxidativo e degeneracao de celulas em algumas areas encefalicas. Para melhor investigar os efeitos da privacao de sono paradoxal (PSP) sobre os processos de degeneracao neuronal foram utilizados o metodo de dark cell de Gallyas e a coloracao de Fluoro-Jade. Foram analisadas as areas e nucleos localizados entre o cortex pre-frontal e a porcao rostral do bulbo em animais submetidos a 96 e 120h de PSP, 96h de PSP somados a 10 ou 24h de recuperacao de sono, 96h de PSP somados a 1g/kg/dia por 6 dias de etanol (ETA) ou 2mg/kgldia por 6 dias de meta-anfetamina (MANF). Observaram-se alteracoes neuropatologicas discretas como resultado da PSP em diversas regioes encefalicas. A analise dos dados nao permitiu evidenciar de forma mais conclusiva a ocorrencia de morte neuronal nestes animais. As alteracoes identificadas podem ser consequencia de dano reversivel. Os processos de lesao neuronal associados a PSP e detectaveis pela tecnica de Gallyas parecem ter curso-temporal proprio nao mais existindo apos 120 h. Os grupos com 10 ou 24 horas de recuperacao nao parecem indicar a necessidade de eventos criticos para a expressao da lesao. 0 ETA reverteu os efeitos vistos apos exposicao as 96h de PSP, visto que nestes animais nao foi observada a marcacao de uma unica celula. Por outro lado, a MANF levou a um padrao especifico de marcacao ao inves de promover um aumento na intensidade de marcacao nas areas afetadas pelo PSP. Nesse sentido, a MANF, assim como o etanol, nao apenas modificou o padrao de marcacao, mas de alguma forma preveniu o dano celular em diversas outras areas. Nossos resultados permitem levantar a hipotese de que a pressao pelo sono, ao inves da privacao de sono, e o fator mais importante no desencadeamento de respostas relacionadas a lesao celular. Outra possibilidade nao excludente e que a privacao de sono NREM seria mais critica que a privacao de sono paradoxal na genese dos fenomenos degenerativos neuronaisBV UNIFESP: Teses e dissertaçõe