Laparoscopic cystogastrostomy in the management of pancreatic pseudocysts

Pancreatic pseudocysts develop following acute or chronic pancreatitis. Majority of the cases resolve spontaneously but some persist beyond six weeks. Active management of pancreatic pseudocysts involves draining of the fluid collection through open surgery, endoscopically, laparoscopically or percutaneously. In our setup, drainage has traditionally been done through open surgery. In this article we present a case of a 61 year old male who developed pancreatic pseudocysts secondary to chronic pancreatitis. Laparoscopic cystogastrostomy was performed and there was no recurrence after three months of follow up. The case presentation highlights the need to offer laparoscopic cystogastrostomy as a method of managing pancreatic pseudocysts in our setup.Keywords: Pancreatic Pseudocyst, Cystogastrostomy, Laparoscopic Drainag

A prototype parabolic trough solar concentrators for steam production

In this work, the potential for a solar-thermal concentrator to produce steam has been studied. Three parabolic trough solar concentrators (PTSCs) of dimensions: -aperture width of 1.2 m, Collector length of 5.8 m and aperture area of 6.95 m2 were investigated. The absorber pipe was a copper tube which carried water as the heat transfer fluid, were designed, fabricated, characterized and their efficiencies compared when closed and when open. The PTSCs´ were made of appropriate materials and were manually tracked. They were designed with principal focus at 0.4 m so that the receiver heat loss is minimized by covering the collectors with glass which was 0.0025 m in thickness. The concentration ratio of the solar concentrators was 128. The concentrator testing was carried out for each of the concentrators. The maximum temperature of steam obtained was 248.3oC while average temperature of steam was produced was 150oC. When closed their efficiencies were: Aluminium sheet reflector PTSC; 55.52 %, Car solar reflector PTSC; 54.65 % and Aluminium foil reflector PTSC; 51.29 %. The open solar concentrator efficiencies were 32.38 %, 34.45 % and 27.74 % respectively. The efficiency of car solar reflector when open was higher than for aluminium sheet since it was less prone to thermal degradation when exposed to weather elements. The results obtained show that production of power using the sun flux is a viable undertaking. The concentrators can be used to provide power to remote areas which are far away from the power transmission gridlines. This will make power readily available to the marginalized rural people. Improvement of the tracking system and optical efficiency can improve the efficiencies of the fabricated concentrator systemsKeywords: Parabolic trough concentrator, solar-thermal, transmittance absorptance product, thermal and optical efficienc

EFFECTS OF CAPITAL STRUCTURE ON AGENCY COSTS IN COMMERCIAL AND SERVICES COMPANIES LISTED AT THE NAIROBI SECURITIES EXCHANGE IN KENYA

Academic and business research has focused on the competing interests of shareholders and managers, as well as the resulting agency fees. It is well known that managers have a strong desire to advance their own interests, such as their pay, the size of their companies, and the value of their securities. According to agency theory, shareholders will pay agency costs in order to reduce these conflicts. According to the free cash flow principle, when a company creates considerable free cash flow, there are significant agency conflicts between stockholders and executives. A number of empirical studies have proposed solutions to this problem, one of which is the use of a capital structure. Numerous studies on the effect of capital structures on agency costs have been conducted, with varying degrees of success. However, the majority of these studies took place in industrialized nations. The primary goal of this study, which lasted from 2012 to 2018, was to determine how capital configuration affected agency expenses in Kenyan commercial and service firms that were listed on the Nairobi Securities Exchange. The study's specific goals included investigating the effects of equity on agency costs, retained earnings on agency costs, long-term debt on agency costs, and firm size as a moderator of the effects of investment configuration on agency costs. A descriptive research method was used in this study to provide a thorough examination of the relationship between capital structure and agency costs. To collect the required seven-year panel data from the entire population of firms, the Nairobi Securities Exchange, the Capital Market Authority's data banks, and the firms' websites were used. These data were then examined at two levels of statistics: descriptive statistics and inferential statistics. In Kenyan commercial and service firms, variations in equity capital, retained earnings, and long-term debts jointly accounted for 65.9% of the variations in agency costs, according to the regression results.JEL: E22; G31; L10  Article visualizations

Youth in Extension and Advisory Services: Rwanda. Developing Local Extension Capacity Project

The overall objective of this youth in extension diagnostic study is to design a pilot engagement in Rwanda to support and strengthen the inclusion of youth in extension – both as providers and recipients of extension services –as a mechanism to both improve the economic opportunities and livelihoods of youth and increase the effectiveness of extension and advisory service systems.In Phase 1, DLEC engaged with USAID country missions that identified engaging youth in agricultural extension as a strategic priority for economic growth and investment. DLEC then identified several countries in which there was buy-in and support from USAID missions to conduct a diagnostic to develop concrete recommendations for a youth-focused engagement. These countries included Guatemala, Niger and Rwanda.For Phase 2, the output is this report. DLEC conducted a landscape analysis, employing a local systems approach and utilize USAID's "5Rs Framework" (Gray et al., 2018) to analyze the roles of certain actors that form a network of relationships whose interactions depend on resources and produce results for youth in EAS. The process of transforming resources into results via interactions of system actors is governed by rules.Methodologies for obtaining the information for this report included: A literature review, key informant interviews, and field and site visits to view programs and talk to stakeholders. Key informants included USAID country partners, government agencies, private sector and civil society that focus on youth in extension. USAID Mission representatives were interviewed to understand Mission priorities for current projects and the Mission country development cooperation strategy (CDCS) as they relate to youth engagement in extension and ongoing or planned programs addressing youth in extension. The report is not meant to give an account of all initiatives in youth and agricultural extension but rather to present a sample of such initiatives, including ones from all the main different types of actors: donor-funded projects, government agencies, educational institutions, international organizations, national and local NGOs, producer organizations and the private sector.

Identification, Characterization and Purification of MSC_0265, a Potential Immunogenic Antigen Homologue of Mycoplasma mycoides subsp. mycoides in Mycoplasma capricolum subsp. capripneumoniae

In silico identification and characterization of vaccine antigens has opened up new frontiers in the field of reverse vaccinology to mitigate the effects of livestock diseases by development of new subunit vaccines. This study aims to characterize, express and purify MSC_0265 for eventual use in immunoassays and inoculation in goats. Mycoplasma mycoides subs. mycoides (Mmm) and Mycoplasma capricolum capripneumoniae (Mccp) are similar pathogens on the genomic level and are the causative agents of Contagious Bovine Pleuropneumonia (CBPP) in cattle and Contagious Caprine Pleuropneumonia (CCPP) in goats respectively. In this study, BLAST was used to identify the homology of MSC_0265 in Mycoplasma capricolum capripneumoniae genome and the protein it is similar to. Characterization of MSC_0265 was also done using I-TASSER to predict secondary structure, solvent accessibility, normalised B-factor, 3D models and function. With cut off points of 0.0 for E-value, 100% for Query coverage and 90% for Identity, MSC_0265 a pyruvate dehydrogenase enzyme gave a high homology score on tBLASTn and BLASTp. It had earlier been cloned in pGS21a vector before proceeding with expression and purification of the His-tagged protein by Ni-NTA affinity chromatography. This study identified the homologue of MSC_0265 as protein WP_029333261.1 in the Mycoplasma capricolum capripneumoniae genome (Accession NZ_LN515398.1) using tBLASTn and BLASTp. Additionally, MSC_0265 was characterized and its optimal expression profile and estimated molecular weight verified

QTL mapping: a conceptual approach to improving cold tolerance at seedling stage in rice (Oryza sativa. L)

Much of what is known about the process of technological innovation in agriculture has yet to be captured in the discussions of abiotic stress plant tolerance as well as rice cold tolerance. The development of research and technological solutions to minimize risks of current abiotic stresses to the plant can lead to two possible outcomes: increase in agricultural productivity and assist the future of plant breeding work. Research efforts about the role of technological development, driven by abiotic stress constraints, are pivotal in making any assertion about the likely tolerance of plant to abiotic stress. Drawing upon the hypothesis of QTL mapping, this research investigates on detection of QTLs for cold tolerance at the seedling stage in rice (Oryza sativa. L), QTLs identified from a BC1F2 breeding population derived from the cross between Chomrongdhan, a donor parent tolerant with Vary botry a susceptible parent, that lead to increase rice productivity in Madagascar. Using a controlled environment and molecular work, out of total 500 BC1F2 segregating plants, 144 plants were used for genotyping based on of visual seedling stage cold tolerance symptom. A total of 4606 SNP markers evenly spread throughout the whole 12 rice genome was used for parental polymorphism survey. The 34% polymorphic markers were used for QTL mapping for cold tolerance at seedling stage. QTL analysis using inclusive composite interval mapping detected four QTLs on chromosome 2 and 10 with phenotypic variances (R2) of 11.11, 7.55, 12.8 and 8.8%, respectively. The position of QTL on chromosome 2 was flanked by 2262412 and 2237404, three other QTLs were detected on chromosome 10 conferred cold tolerances for seedling growth and leaf growth at 14day after recovery and appear to be a novel QTLs. Selected tolerant plant in this research should be useful for the farmers and the markers flanking those identified QTLs should be useful for molecular marker assisted breeding for cold tolerance for the breeder. Keywords: QTL mapping, cold tolerance, seedling, rice

Influence of procurement process on performance of Constituency Development Fund projects in Marakwet East Constituency in Kenya

ABSTRACT Marakwet East Constituency, Rongo, Ikolomani and Hamisi were ranked as worst constituencies among the surveyed constituencies in the management for CDF funds, with Sh58.9 million of the Sh72.5 (81.2% of funds) allocated for Marakwet East Constituency having been mismanaged. This research will be carried out to assess the influence of procurement process on performance of constituency development fund projects with reference to Marakwet East Constituency. The specific objectives of the study were; to find out the effect of accountability on performance of constituency development fund projects, to analyze the effect of integrity on performance of constituency development fund projects, to assess the effect of responsiveness on performance of constituency development fund projects, and to establish the effect of transparency on performance of constituency development fund projects. The study is of benefit to the Marakwet East Constituency, Government of Kenya and other researchers. Secondary data was collected from Marakwet East Constituency reports, Magazines, and other relevant materials. Responses in the questionnaires was then tabulated, coded and processed by use of a computer programmeStatistical package for Social Science (SPSS) version 21 to analyze the data. Descriptive statistics such as mean and standard deviation were used. Tables, pie charts, and graphs were used to present responses and facilitate comparison. The study found out that majority of the respondents indicated that accountability of management influence procurement process to a very high extent .The study concludes that accountability of management influence procurement process and that management accountability influence the procurement process to a very high extent. Finally the study recommends that procurement practice should be responsive to aspirations, expectations and needs of the target society

Assessment of phenotypic and genetic variation against pod borer among a subset of elite pigeonpea (Cajanus cajan) genotypes in Kenya

Molecular marker information supported by quality morphological data facilitates the choice of suitable parents for applied breeding. The main aim of this study was to assess genetic diversity among 55 cultivated yield elite pigeonpea lines using 21 simple sequence repeat (SSR) markers that are well distributed across the genome. Among the 55 pigeonpea genotypes, 16 medium duration were selected and evaluated in the field for response to pod borer resistance in varied agro-ecological zones of Kenya during long rains of April-October cropping season Twenty one primer pairs detected 80 alleles with a mean of 3.9 alleles per locus and polymorphism information content (PIC) ranging from 0.09 to 0.75 averaging to 0.39 suggesting a low genetic diversity. However, marker CcM1820 revealed the highest number of alleles (9) with a PIC value of 0.75. The genotype response to pod borer attack was significant (P≤0.05) with three genotypes (ICEAPs 01541, 01154-2 and 00902) revealing tolerance to pod borer. The markers based on Neighbor Joining, grouped the 55 genotypes into three main clusters based on parentage selection. Most genotypes developed from ICEAP 00068 as the maternal parent were grouped in Cluster I while cluster II comprised of improved genotypes and cluster III comprised genotypes developed from ICPL 87091 as maternal parent. The resistant genotypes identified in the field experiment were grouped in cluster I except ICEAP 00902 which grouped in cluster II. Future studies should focus on broadening genetic base by including more landraces and wild relatives to maximize selection and improve breeding work

Potato cyst nematodes: a new threat to potato production in east Africa

Open Access Journal; Published online: 25 May 2020Potato cyst nematodes (PCN), such as Globodera rostochiensis and Globodera pallida, are quarantine restricted pests of potato causing major yield and financial losses to farmers. G. rostochiensis was first reported from Kenya’s key potato growing area in 2015. We sought to determine the diversity, prevalence and distribution of PCN species across the country by conducting a country-wide survey between 2016 and 2018, which included a more focused, follow-up assessment in three key potato growing counties. A total of 1,348 soil samples were collected from 20 potato growing counties. Information regarding local potato farming practices, potato cultivar use, their diversity and availability was also recorded. PCN cysts were obtained from 968 samples (71.8%) in all the counties surveyed, with Nyandarua County recording the highest PCN field-incidence at 47.6%. The majority of PCN populations, 99.9%, were identified as G. rostochiensis, while G. pallida was recovered from just one field, in a mixed population with G. rostochiensis. Inconsistencies in PCR amplification efficiency was observed for G. rostochiensis using the recommended EPPO primers, compared with ITS primers AB28/TW81, indicating that this protocol cannot be entirely relied upon to effectively detect PCN. Egg density in Nyandarua County varied between 30.6 and 158.5 viable eggs/g soil, with an average egg viability of 78.9 ± 2.8% (min = 11.6%, max = 99.9%). The PCN-susceptible potato cultivar named Shangi was the most preferred and used by 65% of farmers due to its shorter dormancy and cooking time, while imported cultivars (Destiny, Jelly, Manitou, and Markies) with resistance to G. rostochiensis were used by 7.5% of farmers due to unavailability and/or limited access to seeds. Thus, most farmers preferred using their own farm-saved seeds as opposed to purchasing certified seeds. Establishing the distribution and prevalence of PCN and elucidating the local farming practices that could promote the spread of PCN is a necessary precursor to the implementation of any containment or management strategy in the country and ultimately across the region