Bronchodilator, spasmolytic and calcium antagonist activities of Nigella sativa seeds (Kalonji): a traditional herbal product with multiple medicinal uses

Objective: The seeds of Nigella sativa locally known as Kalonji has been used in traditional medicine for the treatment of a variety of diseases including diarrhoea and asthma. The crude extract of N. sativa seeds (Ns.Cr) was studied in vitro for its possible spasmolytic and bronchodilator activities to rationalize the folkloric uses.Methods: Isolated rabbit jejunum and guinea-pig tracheal preparations were set up in Tyrode\u27s and Kreb\u27s solutions respectively and aerated with 5% CO2 in oxygen. Isotonic and isometric responses were measured on Bioscience oscillograph and Grass polygraph respectively.Results: The Ns.Cr caused a dose-dependent (0.1-3.0 mg/ml) relaxation of spontaneous contractions in rabbit jejunum. Ns.Cr also inhibited K(+)-induced contractions in a similar dose range, suggestive of calcium channel blockade (CCB). This effect was confirmed when pretreatment of the tissue with Ns.Cr, produced a dose-dependent shift in the Ca++ dose-response curves to the right similar to that of verapamil, a standard calcium channel blocker. In guinea-pig trachea, it caused relaxation of carbachol-, histamine- or K(+)-induced contractions indicating CCB. Activity-directed fractionation revealed that the CCB activity is concentrated in the petroleum ether fraction, which was found to be approximately 10 times more potent than the crude extract both in jejunum and tracheal preparations.Conclusion: These data indicate that the crude extract of Nigella sativa seeds exhibits spasmolytic and bronchodilator activities mediated possibly through calcium channel blockade and this activity is concentrated in the organic fraction. Its usefulness for diarrhoea and asthma in traditional medicine, appears thus to be based on a sound mechanistic background

Heavy metals and inorganic constituents in medicinal plants of selected Districts of Khyber Pakhtoonkhwa, Pakistan

Heavy metals such as Cr, Fe, Zn, Mn, Ni, Pb, Cu and Cd, and inorganic ions like HCO3-, CO32-, Ca2+, Mg2+, Cl-, Na+, SO42-, NO3-, Fe2+ and F- were investigated in medicinally important plants: Taraxacam officinale, Cichorium intybus and Figonia critica, applying atomic absorption spectrophotometer techniques. In the studied herbs, there were variable amounts of heavy metals and inorganic ions. The purpose of this study was to standardize heavy metals contamination in various indigenous medicinal plants and to create awareness among the public regarding its safe use at collection areas (containing high level of heavy metals) and their adverse health effects.Key words: Medicinal plants, inorganic constituents, heavy metals, atomic absorption spectrophotometer

Analysis of chemical constituents in medicinal plants of selected districts of Pakhtoonkhwa, Pakistan

Alkaloids, flavonoids and saponins were determined in the Teraxacam officinale, Cichorium intybus and Figonia tritica. Quantative determinations of crude alkaloids, flavonoids and saponins (g kg-1) and their percentages (%) were determined in C. intybus, T. officinale and F. critica collected from Kohat, Mardan, Nowshera and Peshawar regions. Thin layer chromatography (TLC) study of the alkaloids, flavonoids and saponins were carried out with different solvent systems and color of the spot and Rf value of each constituent was determined. Among the samples of C. intybus, relatively higher contents of alkaloids (14 g kg-1) were found in the sample collected from Mardan region, followed by the sample gathered from Nowshera which is equal to 13.8g kg-1. Higher contents of flavonoids were found in C. intybus collected from Kohat region which was 23.48 g kg-1. The other remaining samples of C. intybus ranged in the decreasing order of 19.7, 17.2 and 10.0 g kg-1 for the ones collected from Nowshera, Mardan and Peshawar, respectively. The overall saponin contents remained almost low as compared to the alkaloid and flavonoid contents.Key words: Alkaloids, flavonoids, saponins, TLC study, medicinal plants

Comparative analysis of knowledge, attitudes and perceptions about induced abortions among medical and non-medical students of Karachi

Objectve: To determine the knowledge, attitudes and perceptions regarding induced abortions among university students of Karachi and compare these aspects among medical and non-medical students. STUDY Design: Cross sectional opinion survey. Methods: The study is a cross sectional opinion survey of medical and non-medical students from four educational institutions of Karachi using a pretested questionnaire. Results: Of the 381 students interviewed, 201 were medical and 180 were non-medical. More medical students were aware of the correct definition of induced abortion. An encouraging finding was the awareness of our study population about the only legally acceptable circumstance for induced abortion in Pakistan, that is, if conceptus is a risk to maternal health (p \u3c 0.001). More medical students cited complications that were technical like uterine perforation (p \u3c 0.001), cervical trauma (p \u3c 0.001), DIC (p \u3c 0.001), and sepsis (p \u3c 0.001). More medical students thought induced abortion to be a health issue (p=0.001) as compared to non-medical students. Most of the medical students felt the need to modify Pakistan\u27s law regarding abortion (p=0.008) especially for fetal anomalies (13.9%) and rape (7%).CONCLUSION: Though medical students were more knowledgeable about induced abortions as compared to the non-medical population, there is a need to improve awareness regarding induced and unsafe abortions and their consequences

Phytochemical analysis of selected medicinal plants

Four medicinal plants including Ranunculus arvensis, Equisetum ravens, Carathamus lanatus and Fagonia critica were used for the study. All the plants were biologically active and were used for different types of ailments. Keeping in view their importance, this work was carried out to investigate the quantitative determination of their crude phytochemicals, vitamins and protein contents. The quantitative determination of crude phytochemicals (alkaloids, total phenols, flavonoids and saponins) vitamins (riboflavin, vitamin C, niacin and pectin) and protein were determined in the aforementioned herbs. The phytochemicals including alkaloids, total phenols, flavonoids and saponins were determined quantitatively using literature methods. Vitamins were measured using a UV/ visible spectrophotometer (UV- 1601 Shamidzu) and the protein was determined by the Micro KJeldahl’s method (Horwitz et al., 2000). The studied plants showed variable amounts of phytochemicals, vitamins and protein contents. The study is very important, in that it intended to show the contents of the studied medicinal herbs and also provide a scientific data base line which is of particular importance for the local practioners as well as for the local people using these herbs for a variety of body disorders.Key word: Phytochemical analysis, medicinal plants, Pakistan

Dense-CaptionNet : a sentence generation architecture for fine-grained description of image semantics

Automatic image captioning, a highly challenging research problem, aims to understand and describe the contents of the complex scene in human understandable natural language. The majority of the recent solutions are based on holistic approaches where the scene is described as a whole, potentially losing the important semantic relationship of objects in the scene. We propose Dense-CaptionNet, a region-based deep architecture for fine-grained description of image semantics, which localizes and describes each object/region in the image separately and generates a more detailed description of the scene. The proposed network contains three components which work together to generate a fine-grained description of image semantics. Region descriptions and object relationships are generated by the first module, whereas the second one generates the attributes of objects present in the scene. The textual descriptions obtained as an output of the two modules are concatenated to feed as an input to the sentence generation module, which works on encoder-decoder formulation to generate a grammatically correct but single line, fine-grained description of the whole scene. The proposed Dense-CaptionNet is trained and tested using Visual Genome, MSCOCO, and IAPR TC-12 datasets. The results establish a new state-of-the-art when compared with the existing top performing methodologies, e.g., Up-Down-Captioner, Show, Attend and Tell, Semstyle, and Neural Talk, especially on complex scenes

Erectile Dysfunction as an Independent Predictor of Future Cardiovascular Events: The Multi-Ethnic Study of Atherosclerosis

Vascular erectile dysfunction (ED) and cardiovascular disease (CVD) share common risk factors including obesity, hypertension, metabolic syndrome, diabetes mellitus, and smoking. ED and CVD also have common underlying pathological mechanisms, including endothelial dysfunction, inflammation, and atherosclerosis.1 Despite these close relationships, the evidence documenting ED as an independent predictor of future CVD events is limited

A novel digital score for abundance of tumour infiltrating lymphocytes predicts disease free survival in oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) is the most common type of head and neck (H&N) cancers with an increasing worldwide incidence and a worsening prognosis. The abundance of tumour infiltrating lymphocytes (TILs) has been shown to be a key prognostic indicator in a range of cancers with emerging evidence of its role in OSCC progression and treatment response. However, the current methods of TIL analysis are subjective and open to variability in interpretation. An automated method for quantification of TIL abundance has the potential to facilitate better stratification and prognostication of oral cancer patients. We propose a novel method for objective quantification of TIL abundance in OSCC histology images. The proposed TIL abundance (TILAb) score is calculated by first segmenting the whole slide images (WSIs) into underlying tissue types (tumour, lymphocytes, etc.) and then quantifying the co-localization of lymphocytes and tumour areas in a novel fashion. We investigate the prognostic significance of TILAb score on digitized WSIs of Hematoxylin and Eosin (H&E) stained slides of OSCC patients. Our deep learning based tissue segmentation achieves high accuracy of 96.31%, which paves the way for reliable downstream analysis. We show that the TILAb score is a strong prognostic indicator (p = 0.0006) of disease free survival (DFS) on our OSCC test cohort. The automated TILAb score has a significantly higher prognostic value than the manual TIL score (p = 0.0024). In summary, the proposed TILAb score is a digital biomarker which is based on more accurate classification of tumour and lymphocytic regions, is motivated by the biological definition of TILs as tumour infiltrating lymphocytes, with the added advantages of objective and reproducible quantification

A case study for developing eco-efficient street lighting system in Saudi Arabia

It is now well-known phenomenon that energy efficiency has highest short-term pay out period to decrease overall energy consumption. The replacement of conventional lighting technology with innovative lighting solutions can save up to 40 % of lighting energy. The ecological evaluation of street light provision system in King Abdulaziz University (KAU), Jeddah is carried out using Sustainable Process Index (SPI) methodology. This study is carried out selecting three commonly used street illuminating devices i.e. High Pressure Sodium (HPS) lamps, Compact Fluorescent (CF) lamp and Light Emitting Diode (LED). The results show that energy consumption can be decreased by a factor of 1 to 4 by replacing HPS lamp with high efficiency LED lamp. Similarly, environmental assessment results reveal that ecological footprint as well as carbon footprint caused by lighting service can also be lowered by replacing HPS and CF lamps with LED lamps

State-of-the-art and future perspectives of environmentally friendly machining using biodegradable cutting fluids

The use of cutting fluids has played a vital role in machining operations in lubrication and cooling. Most cutting fluids are mineral oil-based products that are hazardous to the environment and the worker, cause severe diseases and pollute the environment. In addition, petroleum resources are becoming increasingly unsustainable. Due to environmental and health issues, legislations have been established to ensure that the consumption of mineral oil is reduced. Consequently, researchers are making efforts to replace these mineral oil-based products. Vegetable oils are grasping attention due to their better lubricating properties, ease of availability, biodegradability, low prices, and non-toxicity. In this study, a detailed review and critical analysis are conducted of the research works involving vegetable oils as cutting fluids keeping in view the shortcomings and possible solutions to overcome these drawbacks. The purpose of the review is to emphasise the benefits of vegetable oil-based cutting fluids exhibiting comparable performance to that of mineral oil-based products. In addition, an appropriate selection of non-edible vegetable oil-based cutting fluids along with optimum cutting parameters to avoid a scanty supply of edible oils is also discussed. According to this research, vegetable oils are capable of substituting synthetic cutting fluids, and this option might aid in the successful and cost-efficient implementation of green machining