Genetic diversity in threatened plant species Alnus nitida (Spach.) Endel

Alnus nitida (Spach) Endl. is an ethnobotanically important threatened plant species. The genetic diversity among the 50 different genotypes of Alnus nitida was carried out using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE) characterization. A considerable amount of genetic diversity (90%) was observed among the genotypes of A. nitida. The protein characterization was carried out on 12% gel electrophoresis. A total of 10 protein bands were detected in A. nitida genotypes. SDS-PAGE procedure is a useful method for the investigation of both genetic diversity and phylogenetic relationship. Especially, B-5 was monomorphic in A. nitida genotypes and was considered as species specific. All other bands/loci were polymorphic. These polymorphic bands displayed 12, 16, 72, 88, 2, 44, 84, 54 and 12 percent variation respectively. In the present examination, the high intra-specific diversity was observed representing SDS-PAGE is a powerful tool for determining the genetically diverse germplasms in A. nitida. The results obtained by this study could be helpful in the identification and selection of desired genotypes of Alnus nitida for conservation programmes in future. Today, there is still a need to assess genetic variation and protect genetic resources, especially of wild species for prospective benefits in plant conservation programmes

Factors Affecting Inpatient Mortality in Elderly People with Acute Kidney Injury

Background: Acute Kidney Injury (AKI) is common in elderly people (EP). There is paucity of data on predictor of mortality in EP with AKI. Objective: This study was done to know more about factors associated with inpatient mortality in EP with AKI. Methods: We retrospectively reviewed medical records of patients aged 65 years or above hospitalized with a diagnosis of AKI at Aga Khan University Hospital, Karachi, between January 2005 and December 2010. Binary logistic regression models were constructed to identify factors associated with mortality in EP with AKI. Results: 431 patients had AKI, with 341 (79.1%) having stage I AKI, 56 (13%) having stage II AKI, and 34 (7.9%) having stage III AKI. Out of 431 patients, 142 (32.9%) died. Mortality increased with increasing severity of AKI. Mortality was 50% (17/34) in AKI stage III, 44.6% (25/56) in AKI stage II, and 29.3% (100/341) in AKI stage I. Factors associated with increased inpatients mortality were presence of stage III AKI (OR: 3.20, P = 0.04, 95% CI: 1.05-9.72), presence of oliguria (OR: 3.42, P = 0.006, 95% CI: 1.42-8.22), and need for vasopressors (OR: 6.90, P \u3c 0.001, 95% CI: 2.42-19.65). Median bicarbonate 18 versus 17 between those who survived and those who died was associated with less mortality (OR: 0.94, P = 0.02, 95% CI: 0.89-0.99). History of hypertension (OR: 0.49, P = 0.03, 95% CI: 0.25-0.95) and high admission creatinine (OR: 0.68, P = 0.01, 95% CI: 0.50-0.91) were also associated with less mortality. Conclusion: Mortality in EP increases with increasing severity of AKI. Presence of stage III AKI, oliguria, and hemodynamic instability needing vasopressor are associated with increased mortality. Increased median bicarbonate, presence of hypertension, and high admission creatinine were various factors associated with decreased inpatient mortality. Increasing age and need for dialysis did not increase mortality in elderly populatio

Predictors of hospital stay and mortality in dengue virus infection-experience from Aga Khan University Hospital Pakistan

Background: Dengue virus infection (DVI) is very common infection. There is scarcity of data on factor associated with increased hospitalstay and mortality in dengue virus infection (DVI). This study was done to know about factors associated with increased hospital stay and mortality in patients admitted with DVI. Results: Out of 532 patients, two third (72.6%) had stay ≤3 days while one third (27.4%) had stay greater than 3 days. The mean length of hospital stay was 3.46 ± 3.45 days. Factors associated with increased hospital stay (\u3e3 days) included AKI (acute kidney injury) (Odd ratio 2.98; 95% CI 1.66-5.34), prolonged prothrombin time (Odd ratio 2.03; 95% CI 1.07-3.84), prolonged activated partial thromboplastin time (aPTT) (Odd ratio 1.80; CI 95% 1.15-2.83) and increased age of \u3e 41.10 years (Odd ratio 1.03; CI 95% 1.01-1.04).Mortality was 1.5%. High mortality was found in those with AKI (P \u3c0.01), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) (P \u3c0.001), respiratory failure (P0.01), prolong PT (P 0.001), prolong aPTT (P0.01) and increased hospital stay (P0.04). Conclusion: Increasing age, coagulopathy and acute kidney injury in patients with DVI is associated with increased hospital stay. Morality was more in patients with AKI, DHF and DSS, respiratory failure, coagulopathy and these patients had more prolonged hospitalization

DESIGN AND OPERATION OF MICROGRID

The need for new generation systems has motivated the development of microgrids. This new concept may provide significant benefits such as losses compensation, achieving high degree of efficiency and reliability to the transmission and distribution networks. This novel idea provides more advantages about Microgrids like general structure and different topologies. Also an original methodology for facilitating its design is proposed. Finally Simulink model of Microgrid is designed and then analyzed.Â

A study of sharp coefficient bounds for a new subfamily of starlike functions

AbstractIn this article, by employing the hyperbolic tangent function tanhz, a subfamily$\mathcal{S}_{\tanh }^{\ast }$Stanh∗of starlike functions in the open unit disk$\mathbb{D}\subset \mathbb{C}$D⊂C:$$\begin{aligned} \mathbb{D}= \bigl\{ z:z\in \mathbb{C} \text{ and } \vert z \vert < 1 \bigr\} \end{aligned}$$D={z:z∈C and |z|<1}is introduced and investigated. The main contribution of this article includes derivations of sharp inequalities involving the Taylor–Maclaurin coefficients for functions belonging to the class$\mathcal{S}_{\tanh }^{\ast }$Stanh∗of starlike functions in$\mathbb{D}$D. In particular, the bounds of the first three Taylor–Maclaurin coefficients, the estimates of the Fekete–Szegö type functionals, and the estimates of the second- and third-order Hankel determinants are the main problems that are proposed to be studied here

Acute kidney injury in lymphoma: a single centre experience

Background. Acute kidney injury (AKI) is a common but least studied complication of lymphoma. Objective. To determine the frequency and predictors of AKI in lymphoma and to study the impact of AKI on hospital stay and mortality. Methods. Retrospective review of medical records of hospitalized lymphoma patients aged ≥14 years between January 2008 and December 2011 was done. Results. Out of 365 patients, AKI was present in 31.8% (116/365). Multivariate logistic regression analysis showed that independent predictors for AKI included sepsis (odds ratio (OR) 3.76; 95% CI 1.83-7.72), aminoglycosides (OR 4.75; 95% CI 1.15-19.52), diuretics (OR 2.96; 95% CI 1.31-6.69), tumor lysis syndrome (OR 3.85; 95% CI 1.54-9.59), and R-CVP regimen (OR 4.70; 95% CI 1.20-18.36). AKI stages 2 and 3 was associated with increased hospital stay (OR 2.01; 95% CI 1.19-3.40). Conclusion. AKI was significantly associated with sepsis, aminoglycoside, diuretics, presence of tumor lysis syndrome, and use of R-CVP regimen. Presence of AKIN (Acute Kidney Injury Network) stages 2 and 3 AKI had increased hospital stay. AKI was also associated with increased mortality

Assessing Nitrate Contamination Risks in Groundwater: A Machine Learning Approach

Groundwater is one of the primary sources for the daily water requirements of the masses, but it is subjected to contamination due to the pollutants, such as nitrate, percolating through the soil with water. Especially in built-up areas, groundwater vulnerability and contamination are of major concern, and require appropriate consideration. The present study develops a novel framework for assessing groundwater nitrate contamination risk for the area along the Karakoram Highway, which is a part of the China Pakistan Economic Corridor (CPEC) route in northern Pakistan. A groundwater vulnerability map was prepared using the DRASTIC model. The nitrate concentration data from a previous study were used to formulate the nitrate contamination map. Three machine learning (ML) models, i.e., Support Vector Machine (SVM), Multivariate Discriminant Analysis (MDA), and Boosted Regression Trees (BRT), were used to analyze the probability of groundwater contamination incidence. Furthermore, groundwater contamination probability maps were obtained utilizing the ensemble modeling approach. The models were calibrated and validated through calibration trials, using the area under the receiver operating characteristic curve method (AUC), where a minimum AUC threshold value of 80% was achieved. Results indicated the accuracy of the models to be in the range of 0.82–0.87. The final groundwater contamination risk map highlights that 34% of the area is moderately vulnerable to groundwater contamination, and 13% of the area is exposed to high groundwater contamination risk. The findings of this study can facilitate decision-making regarding the location of future built-up areas properly in order to mitigate the nitrate contamination that can further reduce the associated health risks

DESIGN AND OPERATION OF MICROGRID

The need for new generation systems has motivated the development of microgrids. This new concept may provide significant benefits such as losses compensation, achieving high degree of efficiency and reliability to the transmission and distribution networks. This novel idea provides more advantages about Microgrids like general structure and different topologies. Also an original methodology for facilitating its design is proposed. Finally Simulink model of Microgrid is designed and then analyzed.

FREQUENCY OF NASOPHARYNGEAL ANGIOFIBROMA IN PATIENTS PRESENTING WITH RECURRENT EPISTAXIS

Objective: To determine the frequency of nasopharyngeal angiofibroma in patients presenting with recurrent epistaxis. Study design: Cross sectional study. Duration and setting: This study was carried out from July 2017 to July 2018 at ENT, Head & Neck Surgery department, Khyber teaching hospital, Peshawar. Material and method: A total number of 93 patients were included in this study. Patients fulfilling the inclusion criteria were collected from the outdoor patient department (OPD) of ENT, head and neck surgery, Khyber teaching hospital, Peshawar. Informed consent for participating in the study was taken from all patients. The patients’ biodata along with hospital registration number were entered on proforma. The patients were assessed initially by history and nasal endoscopic. CT scan nose and PNS with contrast was done and patient admitted and operated for the suspected nasopharyngeal angiofibroma. Biopsy specimen was sent for histopathology to consultant histopathologist in the hospital’s pathology laboratory. Lab reports of the biopsy specimen showing histopathology of nasopharyngeal angiofibroma were reviewed and data entered in the proforma. Results: Out of 93 patients, 32% patients were in age range 10-15 years, 68% patients were in age range 16-20 years. Mean age was 16 years with SD ± 1.26. Fifty seven percent patients had duration of symptoms <1 year while 43% patients had duration of symptoms > 1 year. The incidence of nasopharyngeal angiofibroma was 18%. Conclusion: Our study concludes that the incidence of nasopharyngeal angiofibroma in our setup is 18% which can be due to the increasing number of Afghan refugees. Key words: nasopharyngeal angiofibroma, recurrent epistaxis