Water and Soil Management Practices to Enhance Plant Growth, Berry Development, and Fruit Quality of Northern Highbush Blueberry (Vaccinium corymbosum L.)

Drought and mandatory water restrictions are limiting the availability of irrigation water in many important blueberry growing regions and new strategies are needed to maintain yield and fruit quality with less water. Three potential options for reducing water use, including deficit irrigation, irrigation cut-offs, and crop thinning, were evaluated for 2 years in a mature planting of northern highbush blueberry (Vaccinium corymbosum L. ‘Elliott’). Treatments consisted of no thinning and 50% crop removal in combination with either full irrigation at 100% of estimated crop evapotranspiration (ETsubscript c]), deficit irrigation at 50% ET[subscript c] (applied for the entire growing season), or full irrigation with irrigation cut-off for 4–6 weeks during early or late stages of fruit development. Stem water potential was similar with full and deficit irrigation but, regardless of crop thinning, declined by 0.5–0.6 MPa when irrigation was cut-off early and by > 2.0 MPa when irrigation was cut-off late. In one or both years, the fruiting season was advanced with either deficit irrigation or late cut-off, whereas cutting off irrigation early delayed the season. Yield was not affected by deficit irrigation in plants with a full crop load but was reduced by an average of 35% when irrigation was cut-off late each year. Cutting off irrigation early likewise reduced yield, but only in the second year when the plants were not thinned; however, early cut-off also reduced fruit soluble solids and berry weight by 7% to 24%compared to full irrigation. Cutting off irrigation late produced the smallest and firmest fruit with the highest soluble solids and total acidity among the treatments, as well as the slowest rate of fruit loss in cold storage. Deficit irrigation had the least effect on fruit quality and, based on these results, appears to be the most viable option for maintaining yield with less water (2.5 ML·ha⁻¹ less water per season). A second study was conducted in a 7-year-old field of certified organic highbush blueberry. Two cultivars (‘Duke’ and ‘Liberty’) mulched with either porous polyethylene ground cover (“weed mat”) or yard debris compost topped with sawdust (sawdust+compost) and each fertilized with either feather meal or fish emulsion were evaluated. One-year-old fruiting laterals were randomly-selected at three heights (top, middle, and bottom) on the east and west side of the plants. Bud, flower, and fruit development were monitored through fruit harvest. There was relatively little effect of mulch type or fertilizer source on the measured variables. Fruit harvest occurred ≈8 d after the fruit were fully blue and ranged from 2-25 July 2012 and 26 June-3 July 2013 in ‘Duke’ and from 1-20 Aug. 2012 and 17 July-7 Aug. 2013 in ‘Liberty’. Proportionally more fruit buds occurred on middle laterals than upper and lower laterals. The dates of bud swell and bud break were not affected by cultivar or lateral position. ‘Duke’ and ‘Liberty’ produced 6-8 and 7-9 flowers/bud, respectively. Fruit set was high in both cultivars, averaging ≈95%. However, 13-18% and 29-38 % of the initial set fruit dropped in ’Duke’ and ‘Liberty’ in late May to early June. Fruit ripening was more uniform within clusters in ‘Duke’ than in ‘Liberty’, and average fruit size was similar among harvests in ‘Duke’ but decreased by 25-40% between the first and last harvest in ‘Liberty’. Fruit matured 3−5 d earlier on the east side of the canopy than on the west side. The results suggest that pruning proportionally more on the lower part of the canopy than on the upper part will result in larger fruit at harvest than uniform pruning throughout the bush. The final study was conducted to determine the potential of applying micronized elemental sulfur (S°) by chemigation through the drip system to reduce high soil pH in a new planting of ‘Duke’ blueberry. The S° was mixed with water and injected weekly for 2 months prior to planting, as well as 2 years after planting, atrates of 0, 50, 100 and 150 kg·ha⁻¹ per year, and was compared to the conventional practice of incorporating prilled S° into the soil prior to planting (two applications of 750 kg·ha⁻¹ each). Chemigation quickly reduced soil pH (0-10 cm) within a month from 6.6 with no S° to 6.1 with 50 kg·ha-1 S° and 5.8 with 100 or 150 kg·ha⁻¹ S°. The change was short-term, however, and by May of the following year, soil pH averaged 6.7, 6.5, 6.2, and 6.1 with each increasing rate of S° chemigation, respectively. The conventional treatment, in comparison, averaged 6.6 on the first date and 6.3 on the second date. In July of the following year, soil pH ranged from an average of 6.4 with no S° to 6.2 with 150 kg·ha⁻¹ S° and 5.5 with prilled S°. Soil pH declined thereafter to as low as 5.9 with additional S° chemigation and at lower depths (10-30 cm) was similar to the conventional treatment. None of the treatments had any effect on winter pruning weight in year 1 or on yield, berry weight, and plant dry weight in year 2. Chemigation with S° can be used to quickly reduce soil pH following planting and, therefore, may be a useful practice to correct high pH problems in established blueberry fields. However, it was less effective and more time consuming than applying prilled S° prior to planting

Modelling of land use and land cover changes and prediction using CA-Markov and Random Forest

We used the Cellular Automata Markov (CA-Markov) integrated technique to study land use and land cover (LULC) changes in the Cholistan and Thal deserts in Punjab, Pakistan. We plotted the distribution of the LULC throughout the desert terrain for the years 1990, 2006 and 2022. The Random Forest methodology was utilized to classify the data obtained from Landsat 5 (TM), Landsat 7 (ETM+) and Landsat 8 (OLI/TIRS), as well as ancillary data. The LULC maps generated using this method have an overall accuracy of more than 87%. CA-Markov was utilized to forecast changes in land usage in 2022, and changes were projected for 2038 by extending the patterns seen in 2022. A CA-Markov-Chain was developed for simulating long-term landscape changes at 16-year time steps from 2022 to 2038. Analysis of urban sprawl was carried out by using the Random Forest (RF). Through the CA-Markov Chain analysis, we can expect that high density and low-density residential areas will grow from 8.12 to 12.26 km2 and from 18.10 to 28.45 km2 in 2022 and 2038, as inferred from the changes occurred from 1990 to 2022. The LULC projected for 2038 showed that there would be increased urbanization of the terrain, with probable development in the croplands westward and northward, as well as growth in residential centers. The findings can potentially assist management operations geared towards the conservation of wildlife and the eco-system in the region. This study can also be a reference for other studies that try to project changes in arid are as undergoing land-use changes comparable to those in this study

Levering proteomic analysis of Pseudomonas fluorescens mediated resistance responses in tomato during pathogenicity of Fusarium oxysporum f. sp. oxysporum

The tomato, one of the world’s most extensively cultivated and consumed vegetable crops is negatively impacted by various pathogens. This study aimed to observe the differentially expressed proteins in tomato samples in plant–pathogen-biocontrol interactions. The fungal pathogen associated with wilted plants were isolated and identified based on its morphological and molecular characteristics. Fourteen strains of Pseudomonas fluorescens from agricultural soils were identified and described using biochemical assays, molecular analyses, and screening for antagonistic ability against the Fusarium wilt pathogen. Results demonstrated that the potential of P. fluorescens (TPf12) positively influenced the expression of antagonism against tomato wilt disease. A total of 14 proteins expressed differently were revealed in the 2D-PAGE-MS investigation. Proteins such as nucleoside diphosphate kinase, phenylalanine ammonia-lyase, protein kinase family protein, Ser/Thr protein kinase-like are unchanged in FOL pathogen interaction, but up-regulated in FOL + TPf12 treated roots, and lipid transfer-like protein, and phenylalanine ammonia-lyase were down-regulated in FOL infested roots and upregulated in FOL + TPf12 treated tomato roots. Phenylalanine ammonia-lyase protein expression is commonly found in TPf12 bioenriched roots, and FOL + TPf12 treated roots, indicating its role in response to the application of TPf12 in tomato. A GC–MS analysis was performed to detect the bioactive metabolites synthesized by TPf12. Molecular docking investigations were conducted using the maestro’s GLIDE docking module of the Schrodinger Software program. Among the secondary metabolites, Cyclohexanepropanoic acid, 2-oxo-, methyl ester (CAS), and 3-o-(4-o-Beta-D-Galactopyranosyl-Beta-D-Galactopyraosyl)-2-Acetylamino-2-Deoxy-D-Galactose were shown to be top-ranked with a least docking score against each differently expressed proteins. The profiled molecules expressed differently due to plant-pathogen-biocontrol interactions may be directly or incidentally involved in the wilt disease resistance of tomato plants

The Saudi Critical Care Society practice guidelines on the management of COVID-19 in the ICU: Therapy section

BACKGROUND: The rapid increase in coronavirus disease 2019 (COVID-19) cases during the subsequent waves in Saudi Arabia and other countries prompted the Saudi Critical Care Society (SCCS) to put together a panel of experts to issue evidence-based recommendations for the management of COVID-19 in the intensive care unit (ICU). METHODS: The SCCS COVID-19 panel included 51 experts with expertise in critical care, respirology, infectious disease, epidemiology, emergency medicine, clinical pharmacy, nursing, respiratory therapy, methodology, and health policy. All members completed an electronic conflict of interest disclosure form. The panel addressed 9 questions that are related to the therapy of COVID-19 in the ICU. We identified relevant systematic reviews and clinical trials, then used the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach as well as the evidence-to-decision framework (EtD) to assess the quality of evidence and generate recommendations. RESULTS: The SCCS COVID-19 panel issued 12 recommendations on pharmacotherapeutic interventions (immunomodulators, antiviral agents, and anticoagulants) for severe and critical COVID-19, of which 3 were strong recommendations and 9 were weak recommendations. CONCLUSION: The SCCS COVID-19 panel used the GRADE approach to formulate recommendations on therapy for COVID-19 in the ICU. The EtD framework allows adaptation of these recommendations in different contexts. The SCCS guideline committee will update recommendations as new evidence becomes available

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Dorsolateral musculocutaneous perforators of posterior intercostal artery: An anatomical study

Background: The posterior intercostal artery (PICA) is divided into four segments, vertebral, costal, intermuscular, and rectus, based on the neurovascular branching pattern. Dorsal branches arise from the vertebral segment. Several musculocutaneous perforators and a lateral branch originate from the costal segment. Musculocutaneous branches arise from the intermuscular and rectus segments. The purpose of this study is to describe in detail the musculocutaneous perforators of the costal segment of the posterior intercostal artery. Methods: Fresh cadavers were injected with a modified lead oxide-gelatin mixture. Intercostal spaces (8-11) were dissected in twelve cadavers (six preserved cadavers and six fresh cadavers). Angiograms were assembled with Adobe Photoshop. Two fresh cadavers underwent CT angiography and three dimensional reconstructions of the intercostal perforators were performed using Materialise's Interactive Medical Image Control System (MIMICS). Results: In twelve cadavers, a total of 356 perforators (size > 0.5 mm) were found to arise from the posterior intercostal arteries in 96 intercostal (IC) spaces. 154 perforators (>0.5 mm) were found in the costal segment of the PICA. An average of 6.4 perforators was found in each hemithorax. All perforators were found within 2 cm of the midscapular line. At least one perforator was found in all intercostal spaces. Two or more perforators were found in 40% of the 8th and 9th IC spaces and 60% of the 10th and 11th IC spaces. Perforators were oriented perpendicular to the direction of the muscle fibres of the latissimus dorsi and were usually present one or two intercostal spaces below their origin from the PICA. Conclusions: Perforators of the costal segment of the PICA are described in detail. We propose to call these currently unnamed musculocutaneous perforators "dorsolateral" branches of the PICA, as they are located between dorsal and lateral branches of PICA

Assessment of Heavy Metals Accumulation in Soil and Native Plants in an Industrial Environment, Saudi Arabia

Industrial activities are associated with various heavy metals (HMs) being emitted into the environment, which may pose a threat to humans and animals. The rapid increase in an industrial activity in major cities in Saudi Arabia (SA) has raised concerns regarding the accumulation of HMs in the environment. The aim of this study is to assess the accumulation of HMs in soil and native plants in an industrial environment. We collected 36 surface soil samples and 12 plant species from 12 sites in an industrial city in central SA. The results showed that the HMs content in the soil followed a descending order of (Fe > Ni > Zn > Pb > Cu> Cr > Cd). The enrichment factor (EF) of HMs in the soil ranged from 0.20 to 7336. Up to 100%, 16.6%, and 6.2% of soil samples were extremely highly enriched with Cd, Ni, and Pb, respectively. Plant species Cyperus laevigatus accumulate Cd, Pb, and Ni. Citrullus colocynthis accumulate Cd and Pb in significantly (p < 0.001) higher amounts than other studied species. The Pollution Load Index (PLI) values for the 12 sites ranged from 0.52–1.33 with S5 and S2 PLI >1.0 indicating progressive deterioration of these sites. The Bioaccumulation Factor (BF) ranged from 0.04–2.76 and revealed that some plant species may be candidates for phytoextraction potential. The most promising plant species for phytoextraction and remediation were annuals or perennials such as Malva parviflora, Sisymbrium irio and Citrullus colocynthis, especially for Cr and Cu. This study suggests that these native plant species may be useful for phytoremediation in the area